前  言

大腦復雜的細胞組成和特定的結構，使得體外建模難度極大。近年來大腦類器官概括了大腦發(fā)育的許多關鍵特征，激發(fā)全-球神經學領域相關科研人員的興趣，研究成果不斷的發(fā)表于高分期刊，將腦類器官用于各種生理和病理研究。細胞因子作為類器官培養(yǎng)基中的添加試劑，可以引導細胞按特定器官譜系進行分化。為助力腦類器官的培養(yǎng)與分化，義翹神州可提供人EGF、FGF2、NOG、BMP4等一系列腦類器官培養(yǎng)相關產品。

01 腦類器官研究新進展

目前大多數(shù)腦部模型主要是人類死后腦組織、非人靈長類動物組織或者體外2D細胞。由于資源限制和動物模型的物種差異性等原因，使腦類器官成為潛在研究腦部生理和病理的模型。腦類器官主要源自胚胎干細胞（ESC）或者誘導性多能干細胞（iPSC）。對于體外神經生物學和神經發(fā)育障礙的疾病的研究，也會用到不同部位的腦類器官，比如前腦、中腦、海馬腦類器官等。目前使用腦類器官模型涉及到阿爾茨海默癥、帕金森病、自閉癥、精神分裂等，甚至包括漸凍癥、結節(jié)性硬化癥等罕見病。

ASD, autism spectrum disorders; POMC, pro-opiomelanocortin.

（源自：https://doi.org/10.1002/bies.201900011）

02細胞因子在腦類器官中的應用

細胞因子在類器官培養(yǎng)過程中發(fā)揮重要作用。在多能干細胞（PSC）培養(yǎng)中加入了FGF2構建3D腦類器官模型。對于不同部位的腦類器官的培養(yǎng)，會加入不同的細胞因子，比如對于用于研究脊髓小腦共濟失調疾病的小腦類器官培養(yǎng)中，會加入FGF2、FGF19、SDF1等細胞因子。

BDNF, brain‐derived neurotrophic factor; BMP, bone morphogenetic protein; cAMP, cyclic adenosine monophosphate; EGF, epidermal growth factor; FBS, fetal bovine serum; FGF, fibroblast growth factor; GDNF, glial cell‐derived neurotrophic factor. （源自：https://doi.org/10.1002/bies.201900011）

?義翹神州細胞因子產品數(shù)據(jù)

Human FGF2 Protein, Cat: GMP-10014-HNAE 

高純度：

≥ 95 % as determined by SDS-PAGE.

結合活性

Cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The specific activity is >1,000 IU/μg.

Human Noggin Protein, Cat: 10267-HNAH

高純度：

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

高批間一致性

Inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.

☆：SDS-PAGE & SEC-HPLC

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