In order for biodiesel to be used as a motor fuel or blended with petroleum diesel, it must conform to standard specifications (ASTM D 6751 or EN-14214). There are standard GC methods in use today to determine if biodiesel conforms to the standard specifications, one of which is EN-14103, used to determine the ester and linoleic acid methyl ester content. Other methods include EN-14105 / ASTM D 6584 (free and total glycerine and mono, di and triglyceride content) and EN-14110 (residual methanol). Bruker has designed GC solutions for each of these standard methods. This paper describes work conducted on the Bruker 450 gas chromatograph to analyze biodiesel (B-100) in accordance with EN-14103. EN-14103 is used to verify that the ester content of Fatty Acid Methyl Esters (FAME) is greater than 90 % (m/m) and that the linolenic acid content is between 1 % (m/m) and 15 % (m/m) consistent with the EN-14214 specifications. This method is suitable for FAME which contains methyl esters between C14 and C24. 450-GC 多功能氣相色譜儀