Abstract A simple and rapid method using micellar electrokinetic capillary chromatography (MEKC) was developed for the separation and determination of acrylamide in potato chips at low levels for the first time. The experimental conditions for the separation and quantification of acrylamide were optimized at first. The optimized conditions were: 50 mmol/L Na2B4O7 and 40 mmol/L SDS at pH 10.0, 12 kV applied voltage, 76 cm total length (67 cm effective length) and 75 m i.d. capillary, 198 nm wavelength, 15 cm high 25 s hydrodynamics sample injection, 20 ◦C air-coo領. The linear response of acrylamide concentration ranges from 0.5 to 100 g/mL with high correlation coefficient (r = 0.9986, n = 9). The LOD andLOQ were estimated to be 0.1 and 0.33 g/mL based on S/N = 3 and 10. The precision values (expressed as R.S.D.) of intra- and inter-day were0.864.35% and 2.619.65%, respectively. Recoveries spiked at levels 2, 20, 60 g/mL ranged between 90.86% and 99.6% with R.S.D. less than6.5%. Finally, the developed method has been applied to the analysis of real samples and has achieved satisfactory results. All of these indicatedthat it was a reliable method for thequantification of acrylamide in potato chips.© 2006 Elsevier B.V. All rights reserved. GX毛細管電泳儀(紫外檢測)CL1030