色譜柱 : TSK-GEL SWXL series 流動(dòng)相 : 20mmol/L Phosphate buffer + 0.3mol/L NaCl (pH7.0) 流速 : 0.5mL/min 檢測(cè) : UV (280nm) 溫度 : 25C 進(jìn)樣體積 : 10L 樣品 : standard protein (high molecular weight) 液相色譜儀 : Agilent 1120 Compact LC (gradient auto system) 樣品組成 : 1. Mouse monoclonal antibody, IgM (M.W.:900KDa, in house, 3g/L) 2. Thyroglobulin (M.W.:667KDa, bovine thyroid, Sigma T1001, 3g/L) 3. Glutamate dehydrogenase (M.W.:280KDa, Wako pure chemicals, Japan, 306-50844, 4g/L) 4. Mouse monoclonal antibody, IgG1 (M.W.:150KDa, in house, 2g/L) 5. Conalbumin (M.W.:47KDa, Wako pure chemicals, Japan, 016-17073, 4g/L)