色譜柱 : TSK-GEL SWXL series 流動(dòng)相 :20mmol/L phosphate buffer + 0.3mol/L NaCl (pH7.0) 流速 : 1.0mL/min 檢測(cè) : UV (280nm) 溫度 : 25C 進(jìn)樣體積 : 10L 樣品 : Standard sample 液相色譜儀 : Agilent 1120 Compact LC (gradient auto system) 樣品組成 : 1. Thyroglobulin (M.W.:640KDa, bovine thyroid, Sigma T1001, 3g/L) 2. Mouse monoclonal antibody (M.W.:150KDa, in house, IgG1,2g/L) 3. Conalbumin (M.W.:47KDa, Wako pure chemicals, Japan, 016-17073, 4g/L) 4. Ribonuclease A (M.W.:13.7KDa, Sigma R4875, 4g/L) 5. Vitamin B12 (M.W.:1355, Wako pure chemicals, Japan, 224-00344, 0.2g