In this application update, SRM 870 was used to evaluate the Acclaim 120 C18, 120 C8, PA, PA II, and 300 C18 columns. These columns are prepared from high purity type B silica and use advanced, and in some cases patented, bonding chemistries. The NIST formulation of SRM 870 was also reformulated so that the five compounds had similar peak heights at 254 nm. Standards with one half and one fifth concentration of each compound in SRM 870 were prepared. Different separation conditions are also shown (i.e., a different mobile phase concentration for the Acclaim 300 C18 column and a different column temperature for the Acclaim 120 C18 column). These are believed to be more valuable for making lot-to-lot and column-to-column comparisons.