The measurement of lipids in a biological matrix to study their responses to diseases, drugs, genetic modifications, and other stimuli requires a Q - TOF system with outstanding resolution, mass accuracy, and sensitivity. In this application, the 6545 Q - TOF and 1290 Infinity LC system are used to analyze an induced sputum lipid extract from a donor with chronic obstructive pulmonary disease (COPD). T he 6545 Q - TOF is tuned by novel Swarm Autotune for optimal performance in the m/z range of the lipids, and data acquired in both positive and negative ionization modes with reference mass correction Agilent 6545 四極桿飛行時間液質聯(lián)用系統(tǒng)