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• Goat anti- Guinea Pig Interleukin-2

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• Goat anti- Rabbit Inhibin B

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• Goat anti- Rabbit Nitric oxide

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• Anti-β-Actin

  Anti-β-Actinβ-肌動蛋白抗體（內(nèi)參抗體）規(guī)格：0.1ml/0.2mlAnti-β-ActinCAS號:932-66-1,1-乙酰環(huán)己烯?CAS號:126-81-8,雙甲酮,CP,96%MouseSolubleEndoglin,ENG/sCD105ELISAKit人發(fā)動蛋白2廠家電話(DNM2)Elisa試劑盒哪里**人細(xì)胞色素P450c21B/21-羥化酶Elisa試劑盒Elisa試劑盒供貨商,(CYP21B)ELISA試劑盒,96孔|48孔小鼠S100蛋白實(shí)驗(yàn)(S-100)Elisa試劑盒*** 大鼠前列腺酸性磷酸酶Elisa試劑盒進(jìn)口Elisa和國產(chǎn)試劑盒的區(qū)別,(PAP)ELISA試劑盒檢測范圍是多少,96孔|48孔大鼠血小板衍生生長因子AB實(shí)驗(yàn)(PDGF-AB)Elisa試劑盒*** CAS號:15510-55-1,十二烷基三苯基溴化膦,98%人細(xì)胞絨毛蛋白/埃茲蛋白廠家電話(cytovillin/ezrin)Elisa試劑盒哪里**人成纖維細(xì)胞生長因子23Elisa試劑盒進(jìn)口Elisa和國產(chǎn)試劑盒的區(qū)別,(FGF-23)ELISA試劑盒檢測范圍是多少,96孔|48孔CAS號:95-01-2,2,4-二羥基苯甲醛,98%Mousemyelinbasicprotein,MBPELISAKit綿羊主要組織相容性復(fù)合體實(shí)驗(yàn)(MHC)Elisa試劑盒*** 小鼠黑色素細(xì)胞抗體廠家電話(MCAb)Elisa試劑盒哪里**小鼠神經(jīng)生長導(dǎo)向因子Slit2Elisa試劑盒*** 人巨噬細(xì)胞移動YZ因子Elisa試劑盒Elisa試劑盒供貨商,(MIF)ELISA試劑盒檢測范圍是多少,96孔|48孔CAS號:52417-22-8,9-氨基吖啶鹽,99%Anti-β-Actin[詳細(xì)]

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• Antibody （OX42）

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• Anti-Streptavidin antibody （FITC）

  ProductoverviewDescriptionRabbitpolyclonaltoStreptavidin（FITC）ConjugationFITCConjugationnotesFluoresceinisothiocyanate（FITC）（MolecularWeight390daltons）AbsorptionWavelength:495nmEmissionWavelength:528nmFluorochrome/ProteinRatio:4.0molesFITCpermoleofRabbitIgGHostspeciesRabbitSpecificityNocrossreactivityoccursagainstAvidin.TestedapplicationsImmunomicroscopy,FlowCytImmunogenStreptavidin[Streptomycesavidinii]TargetRelevanceStreptavidinisatetramericproteinpurifiedfromStreptomycessp.thatbindsverytightlytothevitaminbiotinwithaKdof~10-14mol/l.Thehighaffinityrecognitionofbiotinandbiotinylatedmoleculeshasmadestreptavidinoneofthemostimportantcomponentsindiagnosticsandlaboratorykits.CellularlocalizationCytoplasmicAlternativenamesSAV1antibodySAV2antibodyStreptavidinV1antibodyStreptavidinV2antibodyPropertiesFormLiquidStorageinstructionsStoreat+4°Cshortterm（1-2weeks）.Aliquotandstoreat-20°Cor-80°C.Avoidrepeatedfreeze/thawcycles.Storagebuffer0.02MKPhosphate,0.15MNaCl,0.01%SodiumAzide,pH7.2SeethewebsiteformoreSDSinformationforthisproduct.PurityIgGfractionPurificationnotesThisproductisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysis.Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-rabbitserumandstreptavidin.ClonalityPolyclonalIsotypeIgGApplicationsImmunomicroscopy,FlowCytTherearenonoteslistedasspecifictotheseapplications.Formoreinformationpleaseseethe'generalapplicationnotes'sectionbeloworcheckthewebsite.GeneralapplicationnotesSuitableforimmunomicroscopyandflowcytometryorFACSanalysisaswellasotherantibodybasedfluorescentassaysOurAbpromisetoyou:QualityguaranteedandexperttechnicalsupportIftheproductdoesnotperformasdescribedonthisdatasheet,wewillofferarefundorreplacement.ForfulldetailsoftheAbpromise,pleasevisit[詳細(xì)]

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• Rabbit Anti-PTEN antibody

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  2018-12-11 10:00

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• Goat Anti-Mouse α-glucosidase

  GoatAnti-Mouseα-glucosidaseStorage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheα-glucosidasekitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownα-glucosidaseconcentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theα-glucosidaseantigenandabiotinylatedmonoclonalantibodyspecificforα-glucosidasearesimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.Theintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofα-glucosidasepresentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:400nmol/L1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-α-glucosidase1Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useGoatAnti-Mouseα-glucosidaseMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領(lǐng).?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof400nmol/Lα-glucosidase.Allowstandardtostandfor5?minuteswithgentleswir領(lǐng)priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.400nmol/L（6Standard）Originaldensity50ul。200nmol/L（5Standard）100ul6Standard+100uldiludent100nmol/L（4Standard）100ul5Standard+100uldiludent50nmol/L（3Standard）100ul4Standard+100uldiludent25nmol/L（2Standard）100ul3Standard+100uldiludent12.5nmol/L（1Standard）100ul2Standard+100uldiludent0nmol/LBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofα-glucosidasestandarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-α-glucosidasetoallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.GoatAnti-Mouseα-glucosidaseSUGGESTEDPLATESCHEMEStandardconcentrations（nmol/L）A400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF12.512.5samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0nmol/L[詳細(xì)]

  2018-09-27 10:00

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• Antibody Research 113004 說明書

  HyMAXHybridomaFusion&CloningSupplement20x貨號:113004規(guī)格:100mL產(chǎn)品描述：HyMAX雜交瘤融合與克隆補(bǔ)充劑20x貨號:113004替代名稱：雜交瘤補(bǔ)充劑，雜交瘤克隆因子，HCF，雜交瘤克隆補(bǔ)充物，HCS，雜交瘤克隆融合補(bǔ)體，HCFS，雜交瘤生長補(bǔ)充劑，HGS，雜交瘤融合和克隆加強(qiáng)，雜交瘤融合和克隆補(bǔ)充，HFCS，細(xì)胞培養(yǎng)雜交瘤媒介補(bǔ)充說明：HyMax雜交瘤融合和生長補(bǔ)充劑是含有各種細(xì)胞因子，生長因子和激素的條件培養(yǎng)基的專有混合物。在新的雜交瘤發(fā)育過程中配制成替代飼養(yǎng)細(xì)胞。它也可用于從液氮快速回收冷凍細(xì)胞。融合后細(xì)胞電鍍期間常規(guī)使用飼養(yǎng)細(xì)胞。通常，飼養(yǎng)細(xì)胞為新開發(fā)的克隆提供許多未知的生長因子。這些包括許多涉及同種型轉(zhuǎn)換和B細(xì)胞IgG分泌維持的細(xì)胞因子。從我們多年的雜交瘤發(fā)展經(jīng)驗(yàn)，我們已經(jīng)配制了這種有條件的媒體，支持更好的生長和更高頻率的IgG生產(chǎn)克隆開發(fā)。測試雜交瘤集落形成效率和IgG分泌的維持。用法：加入5mLHyMax20x至95mL您目前用于培養(yǎng)細(xì)胞的工作介質(zhì)，使其占5％。不要使用超過10％的HyMax。繼續(xù)用這種培養(yǎng)基培養(yǎng)細(xì)胞，直到細(xì)胞生長好幾代。一旦細(xì)胞生長良好，通過逐步將百分比逐步降低到1％，0.5％和0％，可以將培養(yǎng)物從HyMax隔離。格式：作為無菌過濾的組織培養(yǎng)物測試，20x濃縮液準(zhǔn)備使用溶液。運(yùn)送到客戶之前切勿凍結(jié)。每個(gè)100mL瓶子足以制作2L工作介質(zhì)，足以完成一個(gè)完整的雜交瘤開發(fā)項(xiàng)目。儲存：建議在4°C至8°C的冰箱中儲存，直至6個(gè)月內(nèi)使用。它可以制成等分試樣，并在-20°C-80°C下冷凍保存，長期在12個(gè)月內(nèi)使用。避免反復(fù)凍融，這可能會導(dǎo)致活動減少或降水。穩(wěn)定性：儲存于冰箱中的4°C至8°C或冷凍儲存12個(gè)月時(shí)6個(gè)月。一旦在30天內(nèi)解凍使用，或根據(jù)需要進(jìn)行等分和儲存。不要重復(fù)凍結(jié)和解凍。運(yùn)送：準(zhǔn)備新鮮（30天內(nèi)），并以藍(lán)色冰袋液體形式運(yùn)送。HyMax與其他產(chǎn)品的第三方獨(dú)立比較：圖-1用于產(chǎn)生IgG產(chǎn)生B細(xì)胞雜交瘤的克隆因子補(bǔ)充物的分析。脾細(xì)胞與SP2/0細(xì)胞融合，培養(yǎng)14天，然后分析來自培養(yǎng)物的上清液。通過抗Fc（γ）ELISA進(jìn)行分析。IgG陽性克隆被鑒定為產(chǎn)生超過25ng/mLIgG的那些。特點(diǎn)無血清生長添加劑低蛋白含量成分完全已知Z高克隆效率優(yōu)良的單克隆抗體生產(chǎn)無需喂養(yǎng)細(xì)胞品名貨號規(guī)格品牌HybridomaFusionandCloningSupplement;(HFCS)1136373500110ml(50x)RocheAppliedScienceRocheAppliedScience品牌的11363735001已停產(chǎn)品名貨號規(guī)格品牌HyMAXHybridomaFusion&CloningSupplement20x113004100mLantibodyresearchHyMAXHybridomaFusionandCloningSupplement,20XCatalogNo.:113004AlternateNames:Hybridomasupplement,Hybridomacloningfactor,HCF,hybridomacloningsupplement,HCS,hybridomacloningfusionsupplement,HCFS,hybridomagrowthsupplement,HGS,hybridomafusionandcloningbooster,hybridomafusionandcloningsupplement,HFCS,CellCultureHybridomaMediaSupplementsDescription:HyMaxHybridomaFusionandGrowthSupplementisaproprietaryblendofconditionedmediacontainingvariouscytokines,growthfactors&hormones.Itisformulatedtoreplacefeedercellsduringnewhybridomadevelopment.Itcanalsobeusedforfastrecoveryoffrozencellsfromliquidnitrogen.Feedercellsareroutinelyusedduringplatingofcellsafterfusion.Typicallyfeedercellsprovidemanyunknowngrowthfactorstothenewlydevelopingclones.TheseincludesmanycytokineswhichareinvolvedinisotypeswitchingandmaintenanceofIgGsecretionbyB-cells.Fromourmanyyearsofexperiencewithhybridomadevelopmentwehaveformulatedthisconditionedmediathatsupportbettergrowth&higherfrequencyofIgGproducingclonedevelopment.ItistestedforhybridomacolonyformingefficiencyandmaintenanceofIgGsecretion.Usage:Add5mLofHyMax20xto95mLofyourworkingmediacurrentlyusedforculturingcellstomake5%.DonotuseHyMaxatmorethan10%.Continuewithculturingcellswiththismediauntilcellshavegrownwellforfewgenerations.Oncecellshavegrownwell,culturemaybeweanedoffofHyMaxbygraduallyreducingthepercentageinstepsto1%,0.5%and0%.Format:Providedasasterilefiltered,tissueculturetested,20xconcentratereadytousesolution.Neverfrozenbeforeshippingtocustomer.Each100mLbottleisenoughtomake2Lofworkingmedia,enoughfordoingacompletehybridomadevelopmentproject.Storage:Itisrecommendedtostoreat4°C-8°Cinrefrigeratoruntilusewithin6months.Itcanbemadeintoaliquotsandfrozenstoredat-20°C-80°Cforlongtermtousewithina12monthperiod.Avoidrepeatedfreezingandthawing,whichmaycauseactivitylossandorshowprecipitation.Stability:6monthswhenstoredat4°C-8°Cinrefrigeratoror12monthswhenstoredfrozen.Oncethawedusewithin30daysoraliquotandstoreasneeded.Donotfreeze&thawrepeatedly.Shipping:Itispreparedfresh(within30days)andshippedinliquidformonblueicepacks.ThirdpartyindependentcomparisonsofHyMaxtootherproducts:Figure-1AnalysisofcloningfactorsupplementsforproductionofIgG-producingBcellhybridomas.SplenocyteswerefusedwithSP2/0cells,culturedfor14daysbeforeanalysisofsupernatantfromthecultures.Analysiswasperformedviaanti-Fc(gamma)ELISA.IgGpositivecloneswereidentifiedasthoseproducingmorethan25ng/mLIgG.我們公司Zda優(yōu)勢是強(qiáng)大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都是價(jià)格Zdi,因?yàn)閮r(jià)格Zdi意味著沒有服務(wù).5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們du家代理的品牌有：AntibodyResearchCorporation，arcticzymes，Biorelevant，AmberGen,Inc.，clemente-associates，clodronateliposomes，ColumbiaBiosciences，enzymeresearch，GeneBridgesGmbH，Genovis，AmberGen,Inc.BiotechnologyGmbH，HaematologicTechnologiesHTIHaemtech，hookelabs，Immudex，InnovativeResearchofAmerica，inspiralis，ListBiologicalLaboratories,Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-FreezBiologicals，pentapharm，progen，ProteinArk，QA-Bio,Inc，QA-Bio,IncQuickZymeBiosciences，Teknova，TriLinkBioTechnologies,Inc.，ZyagenLaboratories等7：我們還是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā)，歡迎合作。[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊

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• Rat myeloperoxidase

  RatmyeloperoxidaseFORRESEARCHUSEONLYDrugNamesGenericName：Ratmyeloperoxidase（MPO）ELISAKit.PurposeThiskitallowsforthedeterminationofMPOconcentrationsinRatserum,bloodplasma,andotherbiologicalfluids.PrincipleoftheassayThekitassayRatMPOlevelinthesample,usePurifiedRatMPOantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddMPOtowells,CombinedMPOantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofMPOinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.MaterialsprovidedwiththekitMaterialsprovidedwiththekit48determinations96determinationsStorageUsermanual11Closureplatemembrane22Sealedbags11Microelisastripplate112-8℃Standard：180U/L0.5ml×1bottle0.5ml×1bottle2-8℃Standarddiluent1.5ml×1bottle1.5ml×1bottle2-8℃HRP-Conjugatereagent3ml×1bottle6ml×1bottle2-8℃Samplediluent3ml×1bottle6ml×1bottle2-8℃ChromogenSolutionA3ml×1bottle6ml×1bottle2-8℃ChromogenSolutionB3ml×1bottle6ml×1bottle2-8℃StopSolution3ml×1bottle6ml×1bottle2-8℃washsolution（20ml×20fold）×1bottle（20ml×30fold）×1bottle2-8℃Specimenrequirements1.serum-coagulationatroomtemperature10-20mins，centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,Ifprecipitationappeared,Centrifugalagain.2.plasma-usesuitedEDTAorcitrateorasananticoagulant,mix10-20mins,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,Ifprecipitationappeared,Centrifugalagain.3.Urine-collectsueasterilecontainer,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,Ifprecipitationappeared,Centrifugalagain.TheOperationofHydrothoraxandcerebrospinalfluidReferencetoit.4.cellculturesupernatant-detectsecretorycomponents,collectsueasterilecontainer,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,detectthecompositionofcells,DilutcellsuspensionwithPBS（PH7.2-7.4）,Cellconcentrationreached1million/ml,repeatedfreeze-thawcycles,damagecellsandreleaseofintracellularcomponents,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant,Ifprecipitationappeared,Centrifugalagain.5.Tissuesamples-Aftercuttingsamples,checktheweight,addPBS（PH7.2-7.4）,Rapidlyfrozenwithliquidnitrogen,maintainsamplesat2-8℃aftermelting,addPBS（PH7.4）,HomogenizedbyhandorGrinders,centrifugation20-minatthespeedof2000-3000r.p.m.removesupernatant.6.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.7.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.DiluteandaddsampletoStandard:set10StandardwellsontheELISAplatescoated,addStandard100μltothefirstandthesecondwell,thenaddStandarddilution50μltothefirstandthesecondwell,mix;takeout100μlformthefirstandthesecondwellthenaddittothethirdandtheforthwellseparately.thenaddStandarddilution50μltothethirdandtheforthwell,mix;thentakeout50μlfromthethirdandtheforthwelldiscard,add50μltothefifthandthesixthwell,thenaddStandarddilution50μltothefifthandthesixthwell,mix;takeout50μlfromthefifthandthesixthwellandaddtotheseventhandtheeighthwell,thenaddStandarddilution50μltotheseventhandtheeighthwell,mix;takeout50μlfromtheseventhandtheeighthwellandaddtotheninthandthetenthwell,addStandarddilution50μltotheninthandthetenthwell,mix,takeout50μlfromtheninthandthetenthwelldiscard（addSample50μltoeachwellafterDiluting,（density:120U/L,80U/L,40U/L,20U/L,10U/L）2.addsample：Setblankwellsseparately（blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame）.testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl（samplefinaldilutionis5-fold）,addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold（or20-fold）washsolutiondiluted30-fold（or20-fold）withdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction（thebluecolorchangetoyellowcolor）.11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher（ThesampleODisbiggerthanthefirststandardwell）,pleasediluteSample（n-fold）,Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.Takethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.CalculateThischartisforreferenceonlyAssayrange8U/L-150U/LStorageandvalidity1．Storage：2-8℃.2．validity：sixmonths.[詳細(xì)]

  2018-10-23 10:31

  產(chǎn)品樣冊

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• Goat Anti-Pig Interleukin 6

  GoatAnti-PigInterleukin6Storage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheIL-6kitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownIL-6concentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theIL-6antigenandabiotinylatedmonoclonalantibodyspecificforIL-6aresimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-6presentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:800pg/ml1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-IL-61Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領(lǐng).?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof800pg/mlIL-6.Allowstandardtostandfor5?minuteswithgentleswir領(lǐng)priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.800pg/ml（6Standard）Originaldensity50ul。400pg/ml（5Standard）100ul6Standard+100uldiludent200pg/ml（4Standard）100ul5Standard+100uldiludent100pg/ml（3Standard）100ul4Standard+100uldiludent50pg/ml（2Standard）100ul3Standard+100uldiludent25pg/ml（1Standard）100ul2Standard+100uldiludent0pg/mlBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofIL-6standarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-IL-6toallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.SUGGESTEDPLATESCHEMEStandardconcentrations（pg/ml）A800800samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0pg/ml[詳細(xì)]

  2018-09-27 10:00

  產(chǎn)品樣冊

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• Goat interleukin 17(IL-17)

  Goat interleukin 17(IL-17)[詳細(xì)]

  2024-09-28 01:06

  課件

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• Goat Anti-Rabbit Motilin Receptor

  Goat Anti-Rabbit Motilin Receptor[詳細(xì)]

  2024-09-28 10:33

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• Anti-CD4 antibody [mAb51312]抗體說明書

  ProductoverviewDescriptionMousemonoclonal[mAb51312]toCD4HostspeciesMouseTestedapplicationsIHC-P,WB,IHC-FrCrossreactivityReactswithMouse,HumanImmunogenSyntheticpeptideconjugatedtoKLHderivedfromwithinresidues50-150ofHumanCD4.（Note:theaminoacidsequenceisproprietary）（Peptideavailableasab54699.）PositivecontrolThisantibodygaveapositivesignalinHumanThymusandHumanLymphNodeTissueLysateTargetFunctionAccessoryproteinforMHCclass-IIantigen/T-cellreceptorinteraction.MayregulateT-cellactivation.Inducestheaggregationoflipidrafts.SequencesimilaritiesContains3Ig-likeC2-type（immunoglobulin-like）domains.Contains1Ig-likeV-type（immunoglobulin-like）domain.Post-translationalmodificationsPalmitoylationandassociationwithLCKcontributetotheenrichmentofCD4inlipidrafts.CellularlocalizationCellmembrane.Localizestolipidrafts.RemovedfromplasmamembranebyHIV-1Nefproteinthatincreasesclathrin-dependentendocytosisofthisantigentotargetittolysosomaldegradation.Cellsurfaceexpressionisalsodown-modulatedbyHIV-1Envelopepolyproteingp160thatinteractswith,andsequestersCD4intheendoplasmicreticulum.Targetinformationabovefrom:UniProtaccessionP01730TheUniProtConsortiumTheUniversalProteinResource（UniProt）in2010NucleicAcidsRes.38:D142-D148（2010）.AlternativenamesCD4antibodyCD4（L3T4）antibodyCD4antibodyCD4antibodyCD4antigen（p55）antibodyCD4AntigenantibodyCD4moleculeantibodyCD4ReceptorantibodyCD4+Lymphocytedeficiency,includedantibodyCD4_HUMANantibodyCD4mutantibodyL3T4antibodyLeu3antibodyLy-4antibodyLymphocyteantigenCD4antibodyMGC165891antibodyOTTHUMP00000238897antibodyp55antibodyTCellAntigenT4antibodyTcellantigenT4/LEU3antibodyTcelldifferentiationantigenL3T4antibodyTcellOKT4deficiency,includedantibodyhttp://www.abcam.com/CD4-antibody-mAb51312-ab51312.htmlUKoffice:Abcamplc330CambridgeScienceParkCambridgeCB40FLUKTel:+44（0）1223696000Fax:+44（0）1223215215Email:orders@abcam.comUSoffice:AbcamInc.1KendallSquare,SuiteB2304Cambridge,MA02139-1517USATel:（888）77-ABCAM（22226）Fax:（877）774-8286ThesenumbersaretollfreeintheUS/CanadaEmail:us.orders@abcam.comLastupdatedonAugust24,2012TcellsurfaceantigenT4/Leu3antibodyTcellsurfaceantigenT4/Leu3antibodyTCellSurfaceAntigenT4/Leu3antibodyTCellSurfaceGlycoproteinCD4antibodyT-cellsurfaceantigenT4/Leu-3antibodyT-cellsurfaceglycoproteinCD4antibodyW3/25antibodyW3/25antigenantibodyPropertiesFormLiquidStorageinstructionsStoreat+4°Cshortterm（1-2weeks）.Aliquotandstoreat-20°Cor-80°C.Avoidrepeatedfreeze/thawcycles.StoragebufferPreservative:0.02%SodiumAzideConstituents:PBS,pH7.4SeethewebsiteformoreSDSinformationforthisproduct.PurityProteinGpurifiedClonalityMonoclonalClonenumbermAb51312IsotypeIgG1LightchaintypekappaApplicationsIHC-PIHC-P:1/50.WBWB:Useaconcentrationof1-5μg/ml.Detectsabandofapproximately55kDa（predictedmolecularweight:51kDa）.IHC-FrIHC-Fr:Useatanassaydependentconcentration.PubMed:21690251Images（Seethewebsiteforhigherresolutionimagesofthisproduct）OurAbpromisetoyou:QualityguaranteedandexperttechnicalsupportIftheproductdoesnotperformasdescribedonthisdatasheet,wewillofferarefundorreplacement.ForfulldetailsoftheAbpromise,pleasevisithttp://www.abcam.com/abpromiseorcontactourtechnicalteam.[詳細(xì)]

  2018-10-01 10:01

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• Covalently coup領(lǐng) the antibody on an amine-self-as

  Covalently coup領(lǐng) the antibody on an amine-self-as[詳細(xì)]

  2024-09-17 19:14

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• Rat LPS試劑盒

  RatLPS試劑盒RatLPSFORRESEARCHUSEONLYAssayrange：0.03Eu/L0.8Eu/L96determinationsPurposeThiskitallowsforthedeterminationofLPSconcentrationsinRatserum,cellculturesupernatesandotherbiologicalfluidsRatLPS試劑盒PrincipleoftheassayThekitassayRatLPSlevelinthesample,usePurifiedRatLPSantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddLPStowells,CombinedLPSantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofRatLPSinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.RatLPS試劑盒Materialsprovidedwiththekit1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（1.6Eu/L）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11ClosureplateRatLPS試劑盒membrane26ChromogenSolutionB6ml×1bottle12Sealedbags1Specimenrequirements1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevant2literature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.2.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.Assayprocedure1.Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:0.8Eu/L5Standard150μlOriginaldensityStandard+150μlStandarddiluent0.4Eu/L4Standard150μl5Standard+150μlStandarddiluent0.2Eu/L3Standard150μl4Standard+150μlStandarddiluent0.1Eu/L2Standard150μl3Standard+150μlStandarddiluent0.05Eu/L1Standard150μl2Standard+150μlStandarddiluent2.addsample：Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-fold(or20-fold)washsolutiondiluted30-fold(or20-fold)withdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction(thebluecolorchangetoyellowcolor).11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.RatLPS試劑盒3StepsdescriptionStandard,SamplediluentAddStandard,Samplediluent,incubatefor30minat37℃.Wash5time,AddHRP-Conjugatereagent,incubatefor30minat37℃.Wash5times,AddChromogenSolutionAandB,incubatefor30minat37℃.AddStoppSolutionReadabsorbanceat450nmwithin15mincalculateCalculateTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthe4sampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.Importantnotes1.Thekittakesoutfromtherefrigerationenvironment首ldbebalanced15-30minutesintheroomtemperature,ELISAplatescoatedifhasnotuseupafteropened,theplate首ldbestoredinSealedbag.2.washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.3.addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.4.ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.5.Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.6.Thesubstrateevadethelightpreservation.7.Pleaseaccordingtouseinstructionstrictly,Thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.8.Allsamples,washingbufferandeachkindofreject首ldaccordingtoinfectivematerialprocess.9.Donotmixreagentswiththosefromotherlots.RatLPS試劑盒Storageandvalidity1．Storage：2-8℃.2．validity：sixmonths[詳細(xì)]

  2018-10-31 10:00

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• Goat Anti-Dog Tumor necrosis factor

  Goat Anti-Dog Tumor necrosis factor [詳細(xì)]

  2014-04-22 00:00

  安裝說明

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• Goat Anti-Mouse IgG-HRP 二抗

  GoatAnti-MouseIgG-HRPabmart艾比瑪特二抗上海橋星生物銷售直線：021-65672052（蔣先生）咨詢電話：18221794820客服QQ：1468746680上海橋星產(chǎn)品僅供科研，請勿挪作他用。產(chǎn)品名稱：GoatAnti-MouseIgG-HRP品牌：abmart艾比瑪特貨號：M21001規(guī)格：S-100μl應(yīng)用范圍：WB,ELISA,DotBlot相關(guān)產(chǎn)品：ABMART二抗/Beads抗體CatalogResearchareaAntibody抗體Isotype同種型Specificity專一性Application用途Catalog目錄Package規(guī)格Price定價(jià)二抗GoatAnti-MouseIgG-HRPGoat-WB,ELISA,DotBlotM21001S-100μl120L-1ml900GoatAnti-Rabbit&MouseIgG-HRPGoat-WB,ELISA,DotBlotM21003S-100μl120L-1ml900GoatAnti-RabbitIgG-HRPGoat-WB,ELISA,DotBlotM21002S-100μl120L-1ml900Beads抗體Anti-Myc-TagmAb(Agaroseconjugated)MouseAllIPM20012S-500μl2,200L-5ml9000Anti-HA-TagmAb(Agaroseconjugated)MouseAllIPM20013S-500μl2,200L-5ml9000Anti-DYKDDDDK-TagmAb(Agaroseconjugated)(SameasSigma'sAnti-FLAG)MouseAllIPM20018S-500μl2200L-5ml9000ReactivityKey:Hhuman,Mmouse,Rrat,Mkmonkey,DmD.melanogaster,DgDog,ChHmChinesehamster,AllallspeciesexpectedGoatAnti-MouseIgG-HRPabmart艾比瑪特二抗上海橋星生物客服電話：021-65672052手機(jī)：18221794820公司簡介：上海橋星生物提供優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、美國聯(lián)合碳化和美國光譜醫(yī)學(xué)透析袋、培養(yǎng)基和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和Elisa試劑盒及常用生物試劑等，Sigma、Amresco等各大量現(xiàn)貨火爆促銷中，歡迎登錄www.bridge-star.com或撥打021-65672052、18221794820[詳細(xì)]

  2019-01-01 10:01

  產(chǎn)品樣冊

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• Rat Aβ1-42 ELISA kit

  www.biokanu.comRatamyloidbetapeptide1-42（Aβ1-42）ELISAKitProd.No.3R285FROM:RBForthequantitativeinvitrodeterminationofAβ1-42concentrationsinRatsupernates,serum,plasmaandtissue.FORRESEARCHUSEONLY.NOTFORUSEINDIAGNOSTICPROCEDURES.TABLEOFCONTENTSContentsPageTABLEOFCONTENTS..2INTENDEDUSE..3PRINCIPLE..3WARNINGSANDPRECAUTIONS..4MATERIALSPROVIDEDWITHTHEKIT.7MATERIALSREQUIREDBUTNOTPROVIDED..7STORAGECONDITIONS..8REAGENTPREPARATION..9SPECIMENCOLLECTIONANDPREPARATION..9ASSAYPROCEDURE..10CALCULATIONOFRESULTS..13REFERENCES..14INTENDEDUSEAnenzymeimmunoassayforthequantitativeinvitrodiagnosticmeasurementofRatAβ1-42incellculturesupernates,serum,plasmaandtissue.PRINCIPLEThekitassayRatAβ1-42levelinthesample，usePurifiedRatAβ1-42antibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddAβ1-42towells,CombinedAβ1-42antibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofAβ1-42inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.WARNINGSANDPRECAUTIONSlThiskitisforinvitrodiagnosticuseonly.Forprofessionaluseonly.lAllreagentsofthistestkitwhichcontainhumanserumorplasmahavebeentestedandconfirmednegativeforHIVI/II,HBsAgandHCVbyFDAapprovedprocedures.Allreagents,however,首ldbetreatedaspotentialbiohazardsinuseandfordisposal.lBeforestartingtheassay,readtheinstructionscompletelyandcarefully.Usethevalidversionofthepackageinsertprovidedwiththekit.Besurethateverythingisunderstood.lThemicroplatecontainssnap-offstrips.Unusedwellsmustbestoredat2°Cto8°Cinthesealedfoilpouchandusedintheframeprovided.lPipettingofsamplesandreagentsmustbedoneasquicklyaspossibleandinthesamesequenceforeachstep.lUsereservoirsonlyforsinglereagents.Thisespeciallyappliestothesubstratereservoirs.Usingareservoirfordispensingasubstratesolutionthathadpreviouslybeenusedfortheconjugatesolutionmayturnsolutioncolored.Donotpourreagentsbackintovialsasreagentcontaminationmayoccur.lMixthecontentsofthemicroplatewellsthoroughlytoensuregoodtestresults.Donotreusemicrowells.lDonotletwellsdryduringassay;addreagentsimmediatelyaftercompletingtherinsingsteps.lAllowthereagentstoreachroomtemperature（21-26°C）beforestartingthetest.Temperaturewillaffecttheabsorbancereadingsoftheassay.However,valuesforthepatientsampleswillnotbeaffected.lNeverpipetbymouthandavoidcontactofreagentsandspecimenswithskinandmucousmembranes.lDonotsmoke,eat,drinkorapplycosmeticsinareaswherespecimensorkitreagentsarehandled.lWeardisposablelatexgloveswhenhand領(lǐng)specimensandreagents.Microbialcontaminationofreagentsorspecimensmaygivefalseresults.lHand領(lǐng)首ldbedoneinaccordancewiththeproceduresdefinedbyanappropriatenationalbiohazardsafetyguidelineorregulation.lDonotusereagentsbeyondexpirydateasshownonthekitlabels.lAllindicatedvolumeshavetobeperformedaccordingtotheprotocol.Optimaltestresultsareonlyobtainedwhenusingcalibratedpipettesandmicrotiterplatereaders.lDonotmixorusecomponentsfromkitswithdifferentlotnumbers.Itisadvisednottoexchangewellsofdifferentplatesevenofthesamelot.Thekitsmayhavebeenshippedorstoredunderdifferentconditionsandthebindingcharacteristicsoftheplatesmayresultslightlydifferent.lAvoidcontactwithStopSolutioncontaining0.5MH2SO4.Itmaycauseskinirritationandburns.lSomereagentscontainProclin,BNDand/orMITaspreservatives.Incaseofcontactwitheyesorskin,flushimmediatelywithwater.lTMBsubstratehasanirritanteffectonskinandmucosa.Incaseofpossiblecontact,washeyeswithanabundantvolumeofwaterandskinwithsoapandabundantwater.Washcontaminatedobjectsbeforereusingthem.Ifinhaled,takethepersontoopenair.lChemicalsandpreparedorusedreagentshavetobetreatedashazardouswasteaccordingtothenationalbiohazardsafetyguidelineorregulation.lForinformationonhazardoussubstancesincludedinthekitpleaserefertoMaterialSafetyDataSheetsMATERIALSPROVIDEDWITHTHEKITMaterialsprovidedwiththekit96determinationsStorageUsermanual1Closureplatemembrane2Sealedbags1Microelisastripplate12-8℃Standard：900pg/ml0.5ml×1bottle2-8℃Standarddiluent1.5ml×1bottle2-8℃HRP-Conjugatereagent6ml×1bottle2-8℃Samplediluent6ml×1bottle2-8℃ChromogenSolutionA6ml×1bottle2-8℃ChromogenSolutionB6ml×1bottle2-8℃StopSolution6ml×1bottle2-8℃washsolution30×20ml×1bottle2-8℃MATERIALSREQUIREDBUTNOTPROVIDEDlMicroplatereadercapableofmeasuringabsorbanceat450nm.lPrecisionpipettestodeliver2mlto1mlvolumes.l100mland1litergraduatedcylinders.lCalibratedadjustableprecisionpipettes,preferablywithdisposableplastictips.（Amanifoldmulti-channelpipetteisdesirableforlargeassays.）lAbsorbentpaper.l37°Cincubator.lDistilledordeionizedwater.lDataanalysisandgraphingsoftware.Graphpaper:linear（Cartesian）,log-logorsemi-log,orlog-logitasdesired.lTubestopreparestandardorsampledilutions.STORAGECONDITIONSuWhenstoredat2°Cto8°Cunopenedreagentswillretainreactivityuntilexpirationdate.uDonotusereagentsbeyondthisdate.Openedreagentsmustbestoredat2°Cto8°C.uMicrotiterwellsmustbestoredat2°Cto8°C.Oncethefoilbaghasbeenopened,care首ldbetakentocloseittightlyagain.uOpenedkitsretainactivityfor8weeksifstoredasdescribedabove.REAGENTPREPARATIONBringallreagentstoroomtemperaturebeforeuseSPECIMENCOLLECTIONANDPREPARATIONSerum-Useaserumseparatortube（SST）andallowsamplestoclotfor30minutesbeforecentrifugationfor15minutesatapproximately1000xg.Removeserumandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Plasma-CollectplasmausingEDTAorheparinasananticoagulant.Centrifugesamplesfor15minutesat1000xgat2-8°Cwithin30minutesofcollection.Storesamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcycles.Cellculturefluidandotherbiologicalfluids-Removeparticulatesbycentrifugationandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcyclesASSAYPROCEDUREuGeneralRemarkslAllreagentsandspecimensmustbeallowedtocometoroomtemperaturebeforeuse.Allreagentsmustbemixedwithoutfoaming.lOncethetesthasbeenstarted,allsteps首ldbecompletedwithoutinterruption.lUsenewdisposalplasticpipettetipsforeachstandard,controlorsampleinordertoavoidcrosscontamination.lAbsorbanceisafunctionoftheincubationtimeandtemperature.Beforestartingtheassay,itisrecommendedthatallreagentsareready,capsremoved,allneededwellssecuredinholder,etc.Thiswillensureequalelapsedtimeforeachpipettingstepwithoutinterruption.lAsageneralruletheenzymaticreactionislinearlyproportionaltotimeandtemperature.lDetermineabsorptionwithanELISAreaderat450nmagainst620nmasreference.Ifnoreferencewavelengthisavailable,readonlyat450nm.Iftheextinctionofthehigheststandardexceedsthemeasurementrangeofthephotometer,absorptionmustbemeasuredimmediatelyat405nmagainst620nmasreference.uAssayProcedure1.DiluteandaddsampletoStandard:set10StandardwellsontheELISAplatescoated,addStandard100μltothefirstandthesecondwell,thenaddStandarddilution50μltothefirstandthesecondwell,mix;takeout100μlformthefirstandthesecondwellthenaddittothethirdandtheforthwellseparately.thenaddStandarddilution50μltothethirdandtheforthwell,mix;thentakeout50μlfromthethirdandtheforthwelldiscard,add50μltothefifthandthesixthwell,thenaddStandarddilution50μltothefifthandthesixthwell,mix;takeout50μlfromthefifthandthesixthwellandaddtotheseventhandtheeighthwell,thenaddStandarddilution50μltotheseventhandtheeighthwell,mix;takeout50μlfromtheseventhandtheeighthwellandaddtotheninthandthetenthwell,addStandarddilution50μltotheninthandthetenthwell,mix,takeout50μlfromtheninthandthetenthwelldiscard（addSample50μltoeachwellafterDiluting,（density:600pg/ml，400pg/ml，200pg/ml,100pg/ml，50pg/ml）.50pg/ml100pg/ml600pg/ml200pg/ml900pg/ml400pg/ml2.addsample：Setblankwellsseparately（blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame）.testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl（samplefinaldilutionis5-fold）,addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-foldwashsolutiondiluted30-foldwithdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction（thebluecolorchangetoyellowcolor）.11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.CALCULATIONOFRESULTSlCalculatetheaverageabsorbancevaluesforeachsetofstandards,controlsandpatientsamples.lConstructastandardcurvebyplottingthemeanabsorbanceobtainedfromeachstandardagainstits.lconcentrationwithabsorbancevalueonthevertical（Y）axisandconcentrationonthehorizontal（X）axis.lUsingthemeanabsorbancevalueforeachsampledeterminethecorrespondingconcentrationfromthestandardcurve.lAutomatedmethod:TheresultsintheIFUhavebeencalculatedautomaticallyusinga4PL.l（4ParameterLogistics）curvefit.4ParameterLogisticsisthepreferredcalculationmethod.Otherdata.lreductionfunctionsmaygiveslightlydifferentresults.lTheconcentrationofthesamplescanbereaddirectlyfromthisstandardcurve.Sampleswith.lconcentrationshigherthanthatofthehigheststandardhavetobefurtherdiluted.Forthecalculationof.ltheconcentrationsthisdilutionfactorhastobetakenintoaccount.REFERENCESREF:Cat.-No.:/Kat.-Nr.:/No.-Cat.:/Cat.-No.:/N.Cat.:/N.CatLOT:Lot-No.:/Chargen-Bez.:/No.Lot:/Lot-No.:/LoteN.:/Lotton.::No.ofTests:/Kitgre:/Nb.deTests:/No.deDeterm.:/N.deTestes:/Quantitàdeitests::Keepawayfromheatordirectsunlight./VorHitzeunddirekterSonneneinstrahlungschützen./Garderàl’abridelachaleuretdetouteexpositionlumineuse./Manténgasealejadodelcalorolaluzsolardirecta./Manterlongedocalorouluzsolardirecta./Nonesporreairaggisolari.:Readinstructionsbeforeuse./Arbeitsanleitunglesen./Lirelafichetechniqueavantemploi./Lealasinstruccionesantesdeusar./Lerasinstruesantesdeusar./Leggereleistruzioniprimadell’uso.:Storeat:/Lagernbei:/Stockerà:/Almacenea:/Armazenara:/Conservarea:[詳細(xì)]

  2018-09-22 10:00

  產(chǎn)品樣冊

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