資料庫
儀器網>
資料庫>Goat anti- Rabbit Inhibin B
Goat anti- Rabbit Inhibin B
-
本文由 上海信裕生物科技有限公司 整理匯編
2024-09-22 19:49 303閱讀次數(shù)
文檔僅可預覽首頁內容，請下載后查看全文信息！
-
立即下載
Goat anti- Rabbit Inhibin B
登錄或新用戶注冊
請用手機微信掃描下方二維碼
快速登錄或注冊新賬號
微信掃碼，手機電腦聯(lián)動
更多資料
-
Goat anti- Rabbit Inhibin B- Goat anti- Rabbit Inhibin B[詳細]
-
2024-09-22 19:49
安裝說明
-
- Goat anti- Rabbit Nitric oxide
- Goat anti- Rabbit Nitric oxide[詳細]
-
2024-09-28 00:20
期刊論文
-
- Goat anti- Guinea Pig Interleukin-2
- Goat anti- Guinea Pig Interleukin-2[詳細]
-
2014-04-21 00:00
產品樣冊
-
- Rat Anti- Goat Brucella Antibody
- Rat Anti- Goat Brucella Antibody[詳細]
-
2024-09-20 03:03
報價單
-
- 大鼠YZ素B（Inhibin B）ELISA試劑盒
- 電話：021-6533363955229872網址：http://www.westang.com大鼠YZ素B（InhibinB）ELISA試劑盒（用于血清、血漿、細胞培養(yǎng)上清液和其它生物體液內）原理本實驗采用雙抗體夾心ABC-ELISA法。用抗大鼠InhibinB單抗包被于酶標板上，標準品和樣品中的InhibinB與單抗結合，加入生物素化的抗大鼠InhibinB，形成免疫復合物連接在板上，辣根過氧化物酶標記的Streptavidin與生物素結合，加入底物工作液顯藍色，Z后加終止液硫酸，在450nm處測OD值，InhibinB濃度與OD值成正比，可通過繪制標準曲線求出標本中InhibinB濃度。試劑盒組成（2-8℃保存）酶標板（CoatedWells）96孔酶標抗體工作液（EnzymeConjugate）12ml10×標本稀釋液（SampleBuffer）12ml20×濃縮洗滌液（WashBuffer）50ml標準品（Standards）：5ng/瓶2瓶底物工作液（TMBSolution）12ml**抗體工作液（BiotinylatedAntibody）12ml終止液（StopSolution）12ml準備試劑與收集血樣1.收集標本：血清、血漿（EDTA）、細胞培養(yǎng)上清液、組織勻漿等盡早檢測，2-8℃保存48小時；更長時間須冷凍（-20℃或-70℃）保存，避免反復凍融。2.標準品液配制：使用前加入0.5ml蒸餾水混勻，配成10ng/ml的溶液。設標準管8管，**管加標本稀釋液900ul，第二至第八管加入標本稀釋液500ul。在**管中加入10ng/ml的標準品溶液100ul混勻后用加樣器吸出500ul，移至第二管。如此反復作對倍稀釋，從第七管中吸出500ul棄去。第八管為空白對照。3.10×標本稀釋液用蒸餾水作1:10倍稀釋（示例：1ml濃稀釋液+9ml蒸餾水）。4.洗滌液：用重蒸水1:20稀釋（示例：1ml濃縮洗滌液加入19ml的重蒸水）檢測程序1.加樣：每孔各加入標準品或待測樣品100ul，將反應板充分混勻后置37℃120分鐘。2.洗板：用洗滌液將反應板充分洗滌4-6次，向濾紙上印干。3.每孔中加入**抗體工作液100ul。將反應板充分混勻后置37℃60分鐘。4.洗板：同前。5.每孔加酶標抗體工作液100ul。將反應板置37℃30分鐘。6.洗板：同前。7.每孔加入底物工作液100ul，置37℃暗處反應15分鐘。8.每孔加入100ul終止液混勻。9.30分鐘內用酶標儀在450nm處測吸光值。結果計算與判斷1.所有OD值都應減除空白值后再行計算。2.以標準品1000、500、250、125、62.5、31.2、15.6、0pg/ml為橫坐標，OD值為縱坐標，在坐標紙上作圖，畫出標準曲線。3.根據樣品OD值在該曲線圖上查出相應InhibinB含量即可。試劑盒性能1.靈敏度：Z小的InhibinB檢測濃度小于8pg/ml。2.特異性：可同時檢測重組或天然的大鼠InhibinB。不與大鼠其它細胞因子有交叉反應。3.重復性：板內、板見變異系數(shù)均小于10％。注意事項1.以上標準孔及待測樣品均建議做復孔，每次測定應同時做標準曲線。2.洗滌過程很關鍵。洗滌不充分將導致極ng確度誤差及OD值錯誤地升高。3.板條開封后剩余板條要再封好，保持板條干燥。4.本試劑盒宜置4oC冰箱保存。5.本試劑盒僅用于科研，不能用于臨床診斷！[詳細]
-
2018-09-13 10:00
產品樣冊
-
- 人YZ素B（Inhibin B）ELISA試劑盒說明書
- 人YZ素B（InhibinB）ELISA試劑盒（用于血清、血漿、細胞培養(yǎng)上清液和其它生物體液內）原理本實驗采用雙抗體夾心ABC-ELISA法。用抗人InhibinB單抗包被于酶標板上，標準品和樣品中的InhibinB與單抗結合，加入生物素化的抗人InhibinB，形成免疫復合物連接在板上，辣根過氧化物酶標記的Streptavidin與生物素結合，加入底物工作液顯藍色，Z后加終止液，在450nm處測OD值，InhibinB濃度與OD值成正比，可通過繪制標準曲線求出標本中InhibinB濃度。試劑盒組成（2-8℃保存）酶標板（CoatedWells）96孔酶標抗體工作液（EnzymeConjugate）12ml10×標本稀釋液（SampleBuffer）12ml20×濃縮洗滌液（WashBuffer）50ml標準品（Standards）：5ng/瓶2瓶底物工作液（TMBSolution）12ml**抗體工作液（BiotinylatedAntibody）12ml終止液（StopSolution）12ml準備試劑與收集血樣1.收集標本：血清、血漿（EDTA）、細胞培養(yǎng)上清液、組織勻漿等盡早檢測，2-8℃保存48小時；更長時間須冷凍（-20℃或-70℃）保存，避免反復凍融。2.標準品液配制：使用前加入0.5ml蒸餾水混勻，配成10ng/ml的溶液。設標準管8管，**管加標本稀釋液900ul，第二至第八管加入標本稀釋液500ul。在**管中加入10ng/ml的標準品溶液100ul混勻后用加樣器吸出500ul，移至第二管。如此反復作對倍稀釋，從第七管中吸出500ul棄去。第八管為空白對照。3.10×標本稀釋液用蒸餾水作1:10倍稀釋（示例：1ml濃稀釋液+9ml蒸餾水）。4.洗滌液：用重蒸水1:20稀釋（示例：1ml濃縮洗滌液加入19ml的重蒸水）檢測程序1.加樣：每孔各加入標準品或待測樣品100ul，將反應板充分混勻后置37℃120分鐘。2.洗板：用洗滌液將反應板充分洗滌4-6次，向濾紙上印干。3.每孔中加入**抗體工作液100ul。將反應板充分混勻后置37℃60分鐘。4.洗板：同前。5.每孔加酶標抗體工作液100ul。將反應板置37℃30分鐘。6.洗板：同前。7.每孔加入底物工作液100ul，置37℃暗處反應15分鐘。8.每孔加入100ul終止液混勻。9.30分鐘內用酶標儀在450nm處測吸光值。結果計算與判斷1.所有OD值都應減除空白值后再行計算。2.以標準品1000、500、250、125、62.5、31.2、15.6、0pg/ml為橫坐標，OD值為縱坐標，在坐標紙上作圖，畫出標準曲線。3.根據樣品OD值在該曲線圖上查出相應InhibinB含量。試劑盒性能1.靈敏度：Z小的InhibinB檢測濃度小于10pg/ml。2.特異性：可同時檢測重組或天然的人InhibinB。不與人其它細胞因子有交叉反應。3.重復性：板內、板見變異系數(shù)均小于10％。注意事項1.以上標準孔及待測樣品均建議做復孔，每次測定應同時做標準曲線。2.洗滌過程很關鍵。洗滌不充分將導致極ng確度誤差及OD值錯誤地升高。3.板條開封后剩余板條要再封好，保持板條干燥。4.本試劑盒宜置4oC冰箱保存。5.本試劑盒僅用于科研，不能用于臨床診斷！[詳細]
-
2018-09-13 10:01
產品樣冊
-
- Anti-β-Actin
- Anti-β-Actinβ-肌動蛋白抗體（內參抗體）規(guī)格：0.1ml/0.2mlAnti-β-ActinCAS號:932-66-1,1-乙酰環(huán)己烯?CAS號:126-81-8,雙甲酮,CP,96%MouseSolubleEndoglin,ENG/sCD105ELISAKit人發(fā)動蛋白2廠家電話(DNM2)Elisa試劑盒哪里**人細胞色素P450c21B/21-羥化酶Elisa試劑盒Elisa試劑盒供貨商,(CYP21B)ELISA試劑盒,96孔|48孔小鼠S100蛋白實驗(S-100)Elisa試劑盒*** 大鼠前列腺酸性磷酸酶Elisa試劑盒進口Elisa和國產試劑盒的區(qū)別,(PAP)ELISA試劑盒檢測范圍是多少,96孔|48孔大鼠血小板衍生生長因子AB實驗(PDGF-AB)Elisa試劑盒*** CAS號:15510-55-1,十二烷基三苯基溴化膦,98%人細胞絨毛蛋白/埃茲蛋白廠家電話(cytovillin/ezrin)Elisa試劑盒哪里**人成纖維細胞生長因子23Elisa試劑盒進口Elisa和國產試劑盒的區(qū)別,(FGF-23)ELISA試劑盒檢測范圍是多少,96孔|48孔CAS號:95-01-2,2,4-二羥基苯甲醛,98%Mousemyelinbasicprotein,MBPELISAKit綿羊主要組織相容性復合體實驗(MHC)Elisa試劑盒*** 小鼠黑色素細胞抗體廠家電話(MCAb)Elisa試劑盒哪里**小鼠神經生長導向因子Slit2Elisa試劑盒*** 人巨噬細胞移動YZ因子Elisa試劑盒Elisa試劑盒供貨商,(MIF)ELISA試劑盒檢測范圍是多少,96孔|48孔CAS號:52417-22-8,9-氨基吖啶鹽,99%Anti-β-Actin[詳細]
-
2018-10-23 10:31
產品樣冊
-
Goat Anti-Mouse α-glucosidase- GoatAnti-Mouseα-glucosidaseStorage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheα-glucosidasekitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownα-glucosidaseconcentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theα-glucosidaseantigenandabiotinylatedmonoclonalantibodyspecificforα-glucosidasearesimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.Theintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofα-glucosidasepresentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:400nmol/L1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-α-glucosidase1Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useGoatAnti-Mouseα-glucosidaseMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領.?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof400nmol/Lα-glucosidase.Allowstandardtostandfor5?minuteswithgentleswir領priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.400nmol/L（6Standard）Originaldensity50ul。200nmol/L（5Standard）100ul6Standard+100uldiludent100nmol/L（4Standard）100ul5Standard+100uldiludent50nmol/L（3Standard）100ul4Standard+100uldiludent25nmol/L（2Standard）100ul3Standard+100uldiludent12.5nmol/L（1Standard）100ul2Standard+100uldiludent0nmol/LBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofα-glucosidasestandarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-α-glucosidasetoallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.GoatAnti-Mouseα-glucosidaseSUGGESTEDPLATESCHEMEStandardconcentrations（nmol/L）A400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF12.512.5samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0nmol/L[詳細]
-
2018-09-27 10:00
產品樣冊
-
Rabbit Anti-Hepcidin-25- RabbitAnti-Hepcidin-25Cat.Number:By-6372RQuantitysize:0.2ml（1mg/1ml,LyophilizedorLiquid.Buffer=Preservative:15mMSodiumAzide,Constituents:1%BSA,0.01MPBS,pH7.4.Reconstitutewith0.2mlsteriledistilledwater.）Background:Seemstoactasasigna領moleculeinvolvedinthemaintenanceofironhomeostasis.SeemstoberequiredinconjunctionwithHFEtoregulatebothintestinalironabsorptionandironstorageinmacrophages.HasstrongantimicrobialactivityagainstE.coliML35PN.cinereaandweakeragainstS.epidermidis,S.aureusandgroupbstreptococcusbacteria.ActiveagainstthefungusC.albicans.NoactivityagainstP.aeruginosa.Tissuespecificity:Highestexpressioninliverandtoalesserextentinheartandbrain.Lowlevelsinlung,tonsils,salivarygland,trachea,prostategland,adrenalglandandthyroidgland.Secretedintotheurine.Alsoknownas:Hamp;HEPC;HEPC_HUMAN;Hepc20;Hepc25;HEPCIDIN;Hepcidin20;Hepcidin25;Hepcidinantimicrobialpeptide;Hepcidin-20;Hepcidin25;HFE2;HFE2B;LEAP1;LEAP-1;LEAP1;Liverexpressedantimicrobialpeptide;Liver-expressedantimicrobialpeptide1;PLTR;Putativelivertumorregressor.Specificity:RabbitPolyclonalIgG,affinitypurifiedbyProteinA.Reactswith:hu,mo,ratImmunogen:KLHconjugatedsyntheticpeptidederivedfromhuHepcidin-25.Predictedmolecularweight:20-32kDaApplication:WB=1:100-500ELISA=1:500-1000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500Notyettestedinotherapplications.Optimalworkingdilutionsmustbedeterminedbytheenduser.Storage:Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.TheLyophilizedorLiquidantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications[詳細]
-
2018-09-04 10:00
產品樣冊
-
- Goat Anti-Pig Interleukin 6
- GoatAnti-PigInterleukin6Storage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheIL-6kitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownIL-6concentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theIL-6antigenandabiotinylatedmonoclonalantibodyspecificforIL-6aresimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-6presentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:800pg/ml1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-IL-61Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領.?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof800pg/mlIL-6.Allowstandardtostandfor5?minuteswithgentleswir領priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.800pg/ml（6Standard）Originaldensity50ul。400pg/ml（5Standard）100ul6Standard+100uldiludent200pg/ml（4Standard）100ul5Standard+100uldiludent100pg/ml（3Standard）100ul4Standard+100uldiludent50pg/ml（2Standard）100ul3Standard+100uldiludent25pg/ml（1Standard）100ul2Standard+100uldiludent0pg/mlBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofIL-6standarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-IL-6toallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.SUGGESTEDPLATESCHEMEStandardconcentrations（pg/ml）A800800samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0pg/ml[詳細]
-
2018-09-27 10:00
產品樣冊
-
- Goat interleukin 17(IL-17)
- Goat interleukin 17(IL-17)[詳細]
-
2024-09-28 01:06
課件
-
- Goat Anti-Rabbit Motilin Receptor
- Goat Anti-Rabbit Motilin Receptor[詳細]
-
2024-09-28 10:33
其它
-
- Rabbit Anti-Acrp30 FITC
- RabbitAnti-Acrp30FITC[詳細]
-
2018-10-01 10:01
產品樣冊
-
- Rabbit Anti-SHH FITC
- RabbitAnti-SHHFITC[詳細]
-
2018-10-01 10:01
產品樣冊
-
- Rabbit Anti-SHH HRP
- RabbitAnti-SHHHRP[詳細]
-
2018-10-01 10:01
產品樣冊
-
- Rabbit Anti-Acrp30 HRP
- RabbitAnti-Acrp30HRP[詳細]
-
2018-10-01 10:01
產品樣冊
-
- Rabbit Anti-PTEN antibody
- RabbitAnti-PTENantibodyCatalogNumber:BYK-0748RQuantitysize:100ug(0.01MPBS,pH7.4with10mg/mlBSAand0.1%Sodiumazide)Background:Potentialtumorsuppressor.Actsasaphosphoinositide3-phosphatasebyregulatingPtdIns(3,4,5)P3levels.InvolvedinregulationoftheAKT1signa領pathway.TheunphosphorylatedformcooperateswithAIP1tosuppressAKT1activation.ThePTENdiscoversthefirsttohavethesuppressofthephosphoricacidenzymeactivitycancergenecurrently.ThegeneofPTENlocatesthechromosome10q23area,sendingforthsextumorandafewhouseholdscancerswiththevarietytosufferfromthecomprehensivediseaseeasilyrelevant.TheactivitythatpassestorepresstheAktregulatesthecellperiod,thecellgroundruledeceaseandgluestoconnect.ThistextdiscussedPTENstructure,functionanditscorrelationses,thePTENisintumorrepressfunctionmechanism.RabbitAnti-PTENantibodySpecificity:Anti-PTENisarabbitpolyclonalantibodyunconjugatedspecificforPTENofHuman,Mouse,Ratuseforwesternblotting,elisa,immunoprecipitationandimmunohistochemistryProteinGaffinitychromatographypurification,purity:>95%Isotype:IgGmolwt:44kDaApplication:Westernblotting1:100-500Immunohistochemistry1:100-500ELISA1:500-1000IP=1:20-100IF=1:100-500Optimalworkingdilutionsmustbedeterminedbytheenduser.RabbitAnti-PTENantibodyStorage:Storeat-20℃foroneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20℃.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibody,theantibodyisstableforatleastsixweeksat2-4℃ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.RabbitAnti-PTENantibody更多相關抗體：Camk1g(Calcium/calmodulindependentproteinkinaseIG)鈣/鈣調蛋白依賴蛋白激酶IG抗體CaMK2b（calcium/calmodulin-dependentproteinkinaseIIbeita）鈣/鈣調素依賴蛋白激酶2b抗體CAP1/Park7/DJ-1Park7/DJ-1/CAP1抗體CAP2（Adenylylcyclase-associatedprotein2）環(huán)化酶相關蛋白CAP-2抗體CTn1(Cardiactroponin1)心肌肌鈣蛋白抗體CT-1/CTF1(Cardiotrophln1)心肌營養(yǎng)素1抗體CAI(CarbonicanYMdraseI)碳酸酐酶1抗體CTNNAL1(catenin（cadherin-associatedprotein）alpha-like1)粘附分子相關蛋白a1抗體CAⅡ(CarbonicanYMdraseⅡ)碳酸酐酶2抗體CAS（CellularApoptosisSusceptibility）細胞凋亡敏感性基因Caspase-1(ICE/CASP-1/P45)天冬氨酸-胱氨酸特異性蛋白酶家族抗體Caspase-10半胱胺酸蛋白酶-10抗體Caspase-12（ratmouse）半胱胺酸蛋白酶蛋白-12抗體（大、小鼠）Caspase-12（hunman）半胱胺酸蛋白酶蛋白-12抗體()Caspase-13半胱胺酸蛋白酶蛋白-13抗體Caspase-3(Active)/caspase-3p17subunit活化半胱胺酸蛋白酶蛋白-3抗體caspase-3p12subunit活化半胱胺酸蛋白酶蛋白-3抗體procaspase3半胱天冬酶-3酶原抗體CASP4(Caspase-4)半胱胺酸蛋白酶蛋白-4抗體Caspase-6（CT）半胱胺酸蛋白酶蛋白-6抗體（C端）Caspase-6（NT）半胱胺酸蛋白酶蛋白-6抗體(N端)Caspase-6（NT）mouse半胱胺酸蛋白酶蛋白-6抗體(N端)Caspase-8(proMch5)半胱氨酸蛋白酶8抗體Caspase-9白介素1-β轉化酶樣凋亡蛋白酶6抗體Caspase-9白介素1-β轉化酶樣凋亡蛋白酶6抗體[詳細]
-
2018-12-11 10:00
產品樣冊
-
- Rabbit Anti-Tau protein
- RabbitAnti-TauproteinCat.Number:by-0419RQuantitysize:0.1ml（1mg/1ml,LyophilizedorLiquid.Buffer=0.01MPBS,pH7.4with10mg/mlBSAand0.1%Sodiumazide.Reconstitutewith0.1mlsteriledistilledwater.）Background:TauproteinsareimportantPromotesmicrotubuleassemblyandstability,andmightbeinvolvedintheestablishmentandmaintenanceofneuronalpolarity.TheC-terminusbindsaxonalmicrotubuleswhiletheN-terminusbindsneuralplasmamembranecomponents,suggestingthattaufunctionsasalinkerproteinbetweenboth.Axonalpolarityispredeterminedbytaulocalization（intheneuronalcell）inthedomainofthecellbodydefinedbythecentrosome.Theshortisoformsallowplasticityofthecytoskeletonwhereasthelongerisoformsmaypreferentiallyplayaroleinitsstabilization.Tauproteinssubcellularlocatedintheaxonsofneurons,inthecytosolandinassociationwithplasmamembranecomponents.Itexpressedinneurons.PNS-tauisexpressedintheperipheralnervoussystemwhiletheothersareexpressedinthecentralnervoussystem.Alsoknownas:AI413597;ALZ50;AW045860;DDPAC;FLJ31424;FTDP-17;MAPT;MAPTL;MGC134287;MGC138549;MGC156663;MSTD;Mtapt;MTBT1;MTBT2;PHFTAU;PPND;pTau;RNPTAU;TAU;Tau3r;Tau-1;TAU-FACTOR;Tau40;TAU4R.Specificity:RabbitPolyclonalIgG,affinitypurifiedbyProteinA.Reactswith:hu,mo,ratImmunogen:KLHconjugatedsyntheticpeptidederivedfromhumanTauprotein.Predictedmolecularweight:82kDaApplication:WB=1:100-500ELISA=1:500-1000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500Notyettestedinotherapplications.Optimalworkingdilutionsmustbedeterminedbytheenduser.Storage:Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.TheLyophilizedorLiquidantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.[詳細]
-
2024-09-17 01:54
產品樣冊
-
- Goat Anti-Dog Tumor necrosis factor
- Goat Anti-Dog Tumor necrosis factor [詳細]
-
2014-04-22 00:00
安裝說明
-
- Goat Anti-Mouse IgG-HRP 二抗
- GoatAnti-MouseIgG-HRPabmart艾比瑪特二抗上海橋星生物銷售直線：021-65672052（蔣先生）咨詢電話：18221794820客服QQ：1468746680上海橋星產品僅供科研，請勿挪作他用。產品名稱：GoatAnti-MouseIgG-HRP品牌：abmart艾比瑪特貨號：M21001規(guī)格：S-100μl應用范圍：WB,ELISA,DotBlot相關產品：ABMART二抗/Beads抗體CatalogResearchareaAntibody抗體Isotype同種型Specificity專一性Application用途Catalog目錄Package規(guī)格Price定價二抗GoatAnti-MouseIgG-HRPGoat-WB,ELISA,DotBlotM21001S-100μl120L-1ml900GoatAnti-Rabbit&MouseIgG-HRPGoat-WB,ELISA,DotBlotM21003S-100μl120L-1ml900GoatAnti-RabbitIgG-HRPGoat-WB,ELISA,DotBlotM21002S-100μl120L-1ml900Beads抗體Anti-Myc-TagmAb(Agaroseconjugated)MouseAllIPM20012S-500μl2,200L-5ml9000Anti-HA-TagmAb(Agaroseconjugated)MouseAllIPM20013S-500μl2,200L-5ml9000Anti-DYKDDDDK-TagmAb(Agaroseconjugated)(SameasSigma'sAnti-FLAG)MouseAllIPM20018S-500μl2200L-5ml9000ReactivityKey:Hhuman,Mmouse,Rrat,Mkmonkey,DmD.melanogaster,DgDog,ChHmChinesehamster,AllallspeciesexpectedGoatAnti-MouseIgG-HRPabmart艾比瑪特二抗上海橋星生物客服電話：021-65672052手機：18221794820公司簡介：上海橋星生物提供優(yōu)質的分子生物學試劑和試劑盒、美國聯(lián)合碳化和美國光譜醫(yī)學透析袋、培養(yǎng)基和培養(yǎng)耗材、細胞因子和細胞分離液、抗體和Elisa試劑盒及常用生物試劑等，Sigma、Amresco等各大量現(xiàn)貨火爆促銷中，歡迎登錄www.bridge-star.com或撥打021-65672052、18221794820[詳細]
-
2019-01-01 10:01
產品樣冊
Copyright 2004-2026 yiqi.com All Rights Reserved , 未經書面授權 , 頁面內容不得以任何形式進行復制
在線留言
滬公網安備 31011502008050號
參與評論
登錄后參與評論