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• Goat Anti-Rabbit Motilin Receptor

  Goat Anti-Rabbit Motilin Receptor[詳細(xì)]

  2024-09-28 10:33

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• Goat Anti-Mouse α-glucosidase

  GoatAnti-Mouseα-glucosidaseStorage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheα-glucosidasekitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownα-glucosidaseconcentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theα-glucosidaseantigenandabiotinylatedmonoclonalantibodyspecificforα-glucosidasearesimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.Theintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofα-glucosidasepresentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:400nmol/L1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-α-glucosidase1Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useGoatAnti-Mouseα-glucosidaseMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領(lǐng).?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof400nmol/Lα-glucosidase.Allowstandardtostandfor5?minuteswithgentleswir領(lǐng)priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.400nmol/L（6Standard）Originaldensity50ul。200nmol/L（5Standard）100ul6Standard+100uldiludent100nmol/L（4Standard）100ul5Standard+100uldiludent50nmol/L（3Standard）100ul4Standard+100uldiludent25nmol/L（2Standard）100ul3Standard+100uldiludent12.5nmol/L（1Standard）100ul2Standard+100uldiludent0nmol/LBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofα-glucosidasestandarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-α-glucosidasetoallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.GoatAnti-Mouseα-glucosidaseSUGGESTEDPLATESCHEMEStandardconcentrations（nmol/L）A400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF12.512.5samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0nmol/L[詳細(xì)]

  2018-09-27 10:00

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• 大鼠胃動(dòng)素（Motilin）ELISA試劑盒

  大鼠胃動(dòng)素（Motilin）ELISA試劑盒（用于血清、血漿、細(xì)胞培養(yǎng)上清液和其它生物體液內(nèi)）原理本實(shí)驗(yàn)采用雙抗體夾心ABC-ELISA法。用抗大鼠Motilin單抗包被于酶標(biāo)板上，標(biāo)準(zhǔn)品和樣品中的Motilin與單抗結(jié)合，加入生物素化的抗大鼠Motilin，形成免疫復(fù)合物連接在板上，辣根過(guò)氧化物酶標(biāo)記的Streptavidin與生物素結(jié)合，加入底物工作液顯藍(lán)色，Z后加終止液硫酸，在450nm處測(cè)OD值，Motilin濃度與OD值成正比，可通過(guò)繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中Motilin濃度。試劑盒組成（2-8℃保存）酶標(biāo)板（CoatedWells）96孔酶標(biāo)抗體工作液（EnzymeConjugate）12ml10×標(biāo)本稀釋液（SampleBuffer）12ml20×濃縮洗滌液（WashBuffer）50ml標(biāo)準(zhǔn)品（Standards）：40ng/瓶2瓶底物工作液（TMBSolution）12ml**抗體工作液（BiotinylatedAntibody）12ml終止液（StopSolution）12ml準(zhǔn)備試劑與收集血樣1.收集標(biāo)本：血清、血漿（EDTA、檸檬酸鹽、肝素抗凝）、細(xì)胞培養(yǎng)上清液、組織勻漿等盡早檢測(cè)，2-8℃保存48小時(shí)；更長(zhǎng)時(shí)間須冷凍（-20℃或-70℃）保存，避免反復(fù)凍融。2.標(biāo)準(zhǔn)品液配制：使用前加入1ml蒸餾水混勻，配成40ng/ml的溶液。設(shè)標(biāo)準(zhǔn)管8管，**管加標(biāo)本稀釋液900ul，第二至第八管加入標(biāo)本稀釋液500ul。在**管中加入40ng/ml的標(biāo)準(zhǔn)品溶液100ul混勻后用加樣器吸出500ul，移至第二管。如此反復(fù)作對(duì)倍稀釋，從第七管中吸出500ul棄去。第八管為空白對(duì)照。3.10×標(biāo)本稀釋液用蒸餾水作1:10倍稀釋（示例：1ml濃稀釋液+9ml蒸餾水）。4.洗滌液：用重蒸水1:20稀釋（示例：1ml濃縮洗滌液加入19ml的重蒸水）檢測(cè)程序1.加樣：每孔各加入標(biāo)準(zhǔn)品或待測(cè)樣品100ul，將反應(yīng)板充分混勻后置37℃120分鐘。2.洗板：用洗滌液將反應(yīng)板充分洗滌4-6次，向?yàn)V紙上印干。3.每孔中加入**抗體工作液100ul。將反應(yīng)板充分混勻后置37℃60分鐘。4.洗板：同前。5.每孔加酶標(biāo)抗體工作液100ul。將反應(yīng)板置37℃30分鐘。6.洗板：同前。7.每孔加入底物工作液100ul，置37℃暗處反應(yīng)15分鐘。8.每孔加入100ul終止液混勻。9.30分鐘內(nèi)用酶標(biāo)儀在450nm處測(cè)吸光值。結(jié)果計(jì)算與判斷1.所有OD值都應(yīng)減除空白值后再行計(jì)算。2.以標(biāo)準(zhǔn)品4000、2000、1000、500、250、125、62.5、0pg/ml為橫坐標(biāo)，OD值為縱坐標(biāo)，在坐標(biāo)紙上作圖，畫出標(biāo)準(zhǔn)曲線。3.根據(jù)樣品OD值在該曲線圖上查出相應(yīng)Motilin含量。試劑盒性能1.靈敏度：Z小的Motilin檢測(cè)濃度小于30pg/ml。2.特異性：可同時(shí)檢測(cè)重組或天然的大鼠Motilin。不與大鼠其它細(xì)胞因子有交叉反應(yīng)。3.重復(fù)性：板內(nèi)、板見(jiàn)變異系數(shù)均小于10％。注意事項(xiàng)1.以上標(biāo)準(zhǔn)孔及待測(cè)樣品均建議做復(fù)孔，每次測(cè)定應(yīng)同時(shí)做標(biāo)準(zhǔn)曲線。2.洗滌過(guò)程很關(guān)鍵。洗滌不充分將導(dǎo)致極ng確度誤差及OD值錯(cuò)誤地升高。3.板條開(kāi)封后剩余板條要再封好，保持板條干燥。4.本試劑盒宜置4oC冰箱保存。5.本試劑盒僅用于科研，不能用于臨床診斷！[詳細(xì)]

  2018-09-13 10:00

  產(chǎn)品樣冊(cè)

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• Goat Anti-Pig Interleukin 6

  GoatAnti-PigInterleukin6Storage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheIL-6kitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownIL-6concentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theIL-6antigenandabiotinylatedmonoclonalantibodyspecificforIL-6aresimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-6presentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:800pg/ml1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-IL-61Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領(lǐng).?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof800pg/mlIL-6.Allowstandardtostandfor5?minuteswithgentleswir領(lǐng)priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.800pg/ml（6Standard）Originaldensity50ul。400pg/ml（5Standard）100ul6Standard+100uldiludent200pg/ml（4Standard）100ul5Standard+100uldiludent100pg/ml（3Standard）100ul4Standard+100uldiludent50pg/ml（2Standard）100ul3Standard+100uldiludent25pg/ml（1Standard）100ul2Standard+100uldiludent0pg/mlBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofIL-6standarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-IL-6toallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.SUGGESTEDPLATESCHEMEStandardconcentrations（pg/ml）A800800samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0pg/ml[詳細(xì)]

  2018-09-27 10:00

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• Goat interleukin 17(IL-17)

  Goat interleukin 17(IL-17)[詳細(xì)]

  2024-09-28 01:06

  課件

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• 人胃動(dòng)素（Motilin）ELISA試劑盒說(shuō)明書(shū)

  電話：021-6533363955229872網(wǎng)址：http://www.westang.com人胃動(dòng)素（Motilin）ELISA試劑盒（用于血清、血漿、細(xì)胞培養(yǎng)上清液和其它生物體液內(nèi)）原理本實(shí)驗(yàn)采用雙抗體夾心ABC-ELISA法。用抗人Motilin單抗包被于酶標(biāo)板上，標(biāo)準(zhǔn)品和樣品中的Motilin與單抗結(jié)合，加入生物素化的抗人Motilin，形成免疫復(fù)合物連接在板上，辣根過(guò)氧化物酶標(biāo)記的Streptavidin與生物素結(jié)合，加入底物工作液顯藍(lán)色，Z后加終止液硫酸，在450nm處測(cè)OD值，Motilin濃度與OD值成正比，可通過(guò)繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中Motilin濃度。試劑盒組成（2-8℃保存）酶標(biāo)板（CoatedWells）96孔酶標(biāo)抗體工作液（EnzymeConjugate）12ml10×標(biāo)本稀釋液（SampleBuffer）12ml20×濃縮洗滌液（WashBuffer）50ml標(biāo)準(zhǔn)品（Standards）：20ng/瓶2瓶底物工作液（TMBSolution）12ml**抗體工作液（BiotinylatedAntibody）12ml終止液（StopSolution）12ml準(zhǔn)備試劑與收集血樣1.收集標(biāo)本：血清、血漿（EDTA、檸檬酸鹽、肝素抗凝）、細(xì)胞培養(yǎng)上清液、組織勻漿等盡早檢測(cè)，2-8℃保存48小時(shí)；更長(zhǎng)時(shí)間須冷凍（-20℃或-70℃）保存，避免反復(fù)凍融。血清、血漿請(qǐng)做預(yù)實(shí)驗(yàn)以確定稀釋倍數(shù)。2.標(biāo)準(zhǔn)品液配制：使用前加入1ml蒸餾水混勻，配成20ng/ml的溶液。設(shè)標(biāo)準(zhǔn)管8管，**管加標(biāo)本稀釋液900ul，第二至第八管加入標(biāo)本稀釋液500ul。在**管中加入20ng/ml的標(biāo)準(zhǔn)品溶液100ul混勻后用加樣器吸出500ul，移至第二管。如此反復(fù)作對(duì)倍稀釋，從第七管中吸出500ul棄去。第八管為空白對(duì)照。3.10×標(biāo)本稀釋液用蒸餾水作1:10倍稀釋（示例：1ml濃稀釋液+9ml蒸餾水）。4.洗滌液：用重蒸水1:20稀釋（示例：1ml濃縮洗滌液加入19ml的重蒸水）檢測(cè)程序1.加樣：每孔各加入標(biāo)準(zhǔn)品或待測(cè)樣品100ul，將反應(yīng)板充分混勻后置37℃120分鐘。2.洗板：用洗滌液將反應(yīng)板充分洗滌4-6次，向?yàn)V紙上印干。3.每孔中加入**抗體工作液100ul。將反應(yīng)板充分混勻后置37℃60分鐘。4.洗板：同前。5.每孔加酶標(biāo)抗體工作液100ul。將反應(yīng)板置37℃30分鐘。6.洗板：同前。7.每孔加入底物工作液100ul，置37℃暗處反應(yīng)15分鐘。8.每孔加入100ul終止液混勻。9.30分鐘內(nèi)用酶標(biāo)儀在450nm處測(cè)吸光值。結(jié)果計(jì)算與判斷1.所有OD值都應(yīng)減除空白值后再行計(jì)算。2.以標(biāo)準(zhǔn)品2000、1000、500、250、125、62.5、31.2、0pg/ml為橫坐標(biāo)，OD值為縱坐標(biāo)，在坐標(biāo)紙上作圖，畫出標(biāo)準(zhǔn)曲線。3.根據(jù)樣品OD值在該曲線圖上查出相應(yīng)Motilin含量，再乘上稀釋倍數(shù)即可。試劑盒性能1.靈敏度：Z小的Motilin檢測(cè)濃度小于16pg/ml。2.特異性：可同時(shí)檢測(cè)重組或天然的人Motilin。不與人其它細(xì)胞因子有交叉反應(yīng)。3.重復(fù)性：板內(nèi)、板見(jiàn)變異系數(shù)均小于10％。注意事項(xiàng)1.以上標(biāo)準(zhǔn)孔及待測(cè)樣品均建議做復(fù)孔，每次測(cè)定應(yīng)同時(shí)做標(biāo)準(zhǔn)曲線。2.洗滌過(guò)程很關(guān)鍵。洗滌不充分將導(dǎo)致極ng確度誤差及OD值錯(cuò)誤地升高。3.板條開(kāi)封后剩余板條要再封好，保持板條干燥。4.本試劑盒宜置4oC冰箱保存。5.本試劑盒僅用于科研，不能用于臨床診斷！[詳細(xì)]

  2018-09-13 10:01

  產(chǎn)品樣冊(cè)

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• Goat Anti-Dog Tumor necrosis factor

  Goat Anti-Dog Tumor necrosis factor [詳細(xì)]

  2014-04-22 00:00

  安裝說(shuō)明

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• Goat anti- Guinea Pig Interleukin-2

  Goat anti- Guinea Pig Interleukin-2[詳細(xì)]

  2014-04-21 00:00

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• Goat Anti-Mouse IgG-HRP 二抗

  GoatAnti-MouseIgG-HRPabmart艾比瑪特二抗上海橋星生物銷售直線：021-65672052（蔣先生）咨詢電話：18221794820客服QQ：1468746680上海橋星產(chǎn)品僅供科研，請(qǐng)勿挪作他用。產(chǎn)品名稱：GoatAnti-MouseIgG-HRP品牌：abmart艾比瑪特貨號(hào)：M21001規(guī)格：S-100μl應(yīng)用范圍：WB,ELISA,DotBlot相關(guān)產(chǎn)品：ABMART二抗/Beads抗體CatalogResearchareaAntibody抗體Isotype同種型Specificity專一性Application用途Catalog目錄Package規(guī)格Price定價(jià)二抗GoatAnti-MouseIgG-HRPGoat-WB,ELISA,DotBlotM21001S-100μl120L-1ml900GoatAnti-Rabbit&MouseIgG-HRPGoat-WB,ELISA,DotBlotM21003S-100μl120L-1ml900GoatAnti-RabbitIgG-HRPGoat-WB,ELISA,DotBlotM21002S-100μl120L-1ml900Beads抗體Anti-Myc-TagmAb(Agaroseconjugated)MouseAllIPM20012S-500μl2,200L-5ml9000Anti-HA-TagmAb(Agaroseconjugated)MouseAllIPM20013S-500μl2,200L-5ml9000Anti-DYKDDDDK-TagmAb(Agaroseconjugated)(SameasSigma'sAnti-FLAG)MouseAllIPM20018S-500μl2200L-5ml9000ReactivityKey:Hhuman,Mmouse,Rrat,Mkmonkey,DmD.melanogaster,DgDog,ChHmChinesehamster,AllallspeciesexpectedGoatAnti-MouseIgG-HRPabmart艾比瑪特二抗上海橋星生物客服電話：021-65672052手機(jī)：18221794820公司簡(jiǎn)介：上海橋星生物提供優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、美國(guó)聯(lián)合碳化和美國(guó)光譜醫(yī)學(xué)透析袋、培養(yǎng)基和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和Elisa試劑盒及常用生物試劑等，Sigma、Amresco等各大量現(xiàn)貨火爆促銷中，歡迎登錄www.bridge-star.com或撥打021-65672052、18221794820[詳細(xì)]

  2019-01-01 10:01

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• Goat anti- Rabbit Inhibin B

  Goat anti- Rabbit Inhibin B[詳細(xì)]

  2024-09-22 19:49

  安裝說(shuō)明

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• Goat anti- Rabbit Nitric oxide

  Goat anti- Rabbit Nitric oxide[詳細(xì)]

  2024-09-28 00:20

  期刊論文

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• Rat Anti- Goat Brucella Antibody

  Rat Anti- Goat Brucella Antibody[詳細(xì)]

  2024-09-20 03:03

  報(bào)價(jià)單

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