本文由 上海信裕生物科技有限公司 整理匯編

2014-04-22 00:00 431閱讀次數(shù)

收藏 評價 舉報

• 分享

立即下載

參與評論

評論

登錄后參與評論

登錄或新用戶注冊

• 微信登錄
• 密碼登錄
• 短信登錄

請用手機(jī)微信掃描下方二維碼
快速登錄或注冊新賬號

微信掃碼，手機(jī)電腦聯(lián)動

注冊登錄即表示同意《儀器網(wǎng)服務(wù)條款》和《隱私協(xié)議》

賬  號：

密  碼：

圖片碼： 看不清？
換一張？

手機(jī)和郵箱號注冊新賬號>> 忘記密碼？

手機(jī)號：

圖片碼： 看不清？
換一張？

短信碼： 獲取短信碼

手機(jī)和郵箱號注冊新賬號>> 忘記密碼？

更多資料

• Goat Anti-Dog Tumor necrosis factor

  Goat Anti-Dog Tumor necrosis factor [詳細(xì)]

  2014-04-22 00:00

  安裝說明

  |

  • 
  • 
  • 
  • 

• New Advances in Tumor Therapy!

  New Advances in Tumor Therapy![詳細(xì)]

  2024-09-28 01:21

  實驗操作

  |

  • 
  • 
  • 
  • 

• Goat Anti-Mouse α-glucosidase

  GoatAnti-Mouseα-glucosidaseStorage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheα-glucosidasekitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownα-glucosidaseconcentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theα-glucosidaseantigenandabiotinylatedmonoclonalantibodyspecificforα-glucosidasearesimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.Theintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofα-glucosidasepresentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:400nmol/L1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-α-glucosidase1Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useGoatAnti-Mouseα-glucosidaseMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領(lǐng).?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof400nmol/Lα-glucosidase.Allowstandardtostandfor5?minuteswithgentleswir領(lǐng)priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.400nmol/L（6Standard）Originaldensity50ul。200nmol/L（5Standard）100ul6Standard+100uldiludent100nmol/L（4Standard）100ul5Standard+100uldiludent50nmol/L（3Standard）100ul4Standard+100uldiludent25nmol/L（2Standard）100ul3Standard+100uldiludent12.5nmol/L（1Standard）100ul2Standard+100uldiludent0nmol/LBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofα-glucosidasestandarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-α-glucosidasetoallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.GoatAnti-Mouseα-glucosidaseSUGGESTEDPLATESCHEMEStandardconcentrations（nmol/L）A400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF12.512.5samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0nmol/L[詳細(xì)]

  2018-09-27 10:00

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Goat Anti-Pig Interleukin 6

  GoatAnti-PigInterleukin6Storage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheIL-6kitisasolidphasephasesandwichenzymelinkedimmunosorbentassay(ELISA).Samples,includingstandardsofknownIL-6concentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theIL-6antigenandabiotinylatedmonoclonalantibodyspecificforIL-6aresimultaneouslyincubated.Afterwashing,theenzyme(streptavidin-peroxydase)isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-6presentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:800pg/ml1Vials(0.6ml)0.5Vials(0.3ml)Seereagentspreparationonpage3Blankcontrol1Vials(1.0ml)1Vials(0.5ml)Ready-to-useStandardDiluent1Vials(4.0ml)1Vials(2.0ml)Ready-to-useBiotinylatedanti-IL-61Vials(6.0ml)1Vials(3.0ml)Ready-to-useStreptavidin-HRP1Vials(8.0ml)1Vials(4.0ml)Ready-to-useWashingBuffer1Vials(20ml)1Vials(10ml)50×concentrateSubstrateA1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSubstrateB1Vials（6.0ml)1Vials(3.0ml)Ready-to-useStoppingSolution1Vials(6.0ml)1Vials(3.0ml)Ready-to-useSampleDiluent1Vials(12ml)1Vials(6.0ml)Ready-to-useMATERIALREQUIREDBUTNOTPROVIDED?Distilledwater?Pipettes:10ul、50ul、100ul、200ul、1000ul。?Vortexmixerandmagneticstirrer.SAFETY?Forresearchuseonly?AvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.?Donoteat,drink,smokeorapplycosmeticswherekitreagentsareused.?Donotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROL?Whennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.?Oncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.?Coverorcapallreagentswhennotinuse.?Donotmisorinterchangereagentsbetweendifferentlots.?Donotusereagentsbeyondtheexpirationdateofthekit.?Useacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.?Useacleanplasticcontainertopreparethewashingsolution.?Thoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領(lǐng).?Allresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.?TheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.?Whenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.?Respectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGE?Serum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.?Plasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.?Cellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.?Storage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted(250-500ul)toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.?Recommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTS?Standards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof800pg/mlIL-6.Allowstandardtostandfor5?minuteswithgentleswir領(lǐng)priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.800pg/ml（6Standard）Originaldensity50ul。400pg/ml（5Standard）100ul6Standard+100uldiludent200pg/ml（4Standard）100ul5Standard+100uldiludent100pg/ml（3Standard）100ul4Standard+100uldiludent50pg/ml（2Standard）100ul3Standard+100uldiludent25pg/ml（1Standard）100ul2Standard+100uldiludent0pg/mlBlankControl50ul。?Washingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHOD?Beforeuse,mixallreagentsthoroughlywithoutmakingfoam.?Determinethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.?Add50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2(seeplateschemebelow).Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofIL-6standarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.?Dilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..?Add50ulofdilutedbiotinylatedanti-IL-6toallwells.?Coverwithaplatevoverandincubatefor1hourat37℃.?Removethecoverandwashtheplateasfollows:⑴aspiratetheliquidfromeachwell,⑵dispensse0.3mlofwashingsolutionintoeachwell.⑶Aspirateagainthecontetofeachwellafter0.5minute.⑷Repeatsteps⑵and⑶threetimes.?Distribute60ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.?Coverandincubate30minat37℃.?Removethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.?Add50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。?Theenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.?Readabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm(610nmto650nmisacceptable)asthereferencewavelength.SUGGESTEDPLATESCHEMEStandardconcentrations（pg/ml）A800800samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0pg/ml[詳細(xì)]

  2018-09-27 10:00

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Goat interleukin 17(IL-17)

  Goat interleukin 17(IL-17)[詳細(xì)]

  2024-09-28 01:06

  課件

  |

  • 
  • 
  • 
  • 

• Goat Anti-Rabbit Motilin Receptor

  Goat Anti-Rabbit Motilin Receptor[詳細(xì)]

  2024-09-28 10:33

  其它

  |

  • 
  • 
  • 
  • 

• Goat anti- Guinea Pig Interleukin-2

  Goat anti- Guinea Pig Interleukin-2[詳細(xì)]

  2014-04-21 00:00

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Goat Anti-Mouse IgG-HRP 二抗

  GoatAnti-MouseIgG-HRPabmart艾比瑪特二抗上海橋星生物銷售直線：021-65672052（蔣先生）咨詢電話：18221794820客服QQ：1468746680上海橋星產(chǎn)品僅供科研，請勿挪作他用。產(chǎn)品名稱：GoatAnti-MouseIgG-HRP品牌：abmart艾比瑪特貨號：M21001規(guī)格：S-100μl應(yīng)用范圍：WB,ELISA,DotBlot相關(guān)產(chǎn)品：ABMART二抗/Beads抗體CatalogResearchareaAntibody抗體Isotype同種型Specificity專一性Application用途Catalog目錄Package規(guī)格Price定價二抗GoatAnti-MouseIgG-HRPGoat-WB,ELISA,DotBlotM21001S-100μl120L-1ml900GoatAnti-Rabbit&MouseIgG-HRPGoat-WB,ELISA,DotBlotM21003S-100μl120L-1ml900GoatAnti-RabbitIgG-HRPGoat-WB,ELISA,DotBlotM21002S-100μl120L-1ml900Beads抗體Anti-Myc-TagmAb(Agaroseconjugated)MouseAllIPM20012S-500μl2,200L-5ml9000Anti-HA-TagmAb(Agaroseconjugated)MouseAllIPM20013S-500μl2,200L-5ml9000Anti-DYKDDDDK-TagmAb(Agaroseconjugated)(SameasSigma'sAnti-FLAG)MouseAllIPM20018S-500μl2200L-5ml9000ReactivityKey:Hhuman,Mmouse,Rrat,Mkmonkey,DmD.melanogaster,DgDog,ChHmChinesehamster,AllallspeciesexpectedGoatAnti-MouseIgG-HRPabmart艾比瑪特二抗上海橋星生物客服電話：021-65672052手機(jī)：18221794820公司簡介：上海橋星生物提供優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、美國聯(lián)合碳化和美國光譜醫(yī)學(xué)透析袋、培養(yǎng)基和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和Elisa試劑盒及常用生物試劑等，Sigma、Amresco等各大量現(xiàn)貨火爆促銷中，歡迎登錄www.bridge-star.com或撥打021-65672052、18221794820[詳細(xì)]

  2019-01-01 10:01

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Goat anti- Rabbit Inhibin B

  Goat anti- Rabbit Inhibin B[詳細(xì)]

  2024-09-22 19:49

  安裝說明

  |

  • 
  • 
  • 
  • 

• Goat anti- Rabbit Nitric oxide

  Goat anti- Rabbit Nitric oxide[詳細(xì)]

  2024-09-28 00:20

  期刊論文

  |

  • 
  • 
  • 
  • 

• Rat Anti- Goat Brucella Antibody

  Rat Anti- Goat Brucella Antibody[詳細(xì)]

  2024-09-20 03:03

  報價單

  |

  • 
  • 
  • 
  • 

• Human Factor IX 說明書

  EnzymeResearch，EnzymeResearch介紹,EnzymeResearch代理,EnzymeResearch總代,EnzymeResearch**代理,EnzymeResearchZG代理EnzymeResearch（http://www.enzymeresearch.co.uk/）是一家擁有超過25年的酶研究的實驗室，生產(chǎn)和開發(fā)多種用于基本凝血研究的多種酶和輔因子。在過去的十年EnzymeResearchLaboratories為科學(xué)家參與止血和血栓形成的研究提供了寶貴支持。EnzymeResearch通過不斷追求產(chǎn)品的Z高品質(zhì)，贏得了世界各地凝血研究實驗室的信任。EnzymeResearchLaboratories為生物技術(shù)公司,制藥行業(yè)、醫(yī)院和大學(xué)的研究實驗室提供用于全方位的純化抗凝因子。除了其制造能力，EnzymeResearchLaboratories也創(chuàng)新研發(fā)領(lǐng)域中的血液凝固技術(shù)。EnzymeResearch的產(chǎn)品線包括從人類和牛等離子體純化酶原激活酶活躍現(xiàn)場被封鎖的酶凝血因子的單克隆和多克隆抗體檢測凝血因子ELISA試劑自定義的肽和蛋白質(zhì)純化服務(wù)HumanFactorIXHumanFactorIX(ChristmasFactor)人因素IX（圣誕節(jié)因子）貨號:HFIX1009規(guī)格:0.5mg由新鮮的冷凍人血漿制備。ThisproteinpurityisdeterminedbySDS-PAGEandshowsnoreductionuponincubationwith2-mercaptoethanol.Activityisdeterminedviaclottingassay.通過接觸或組織因子途徑激活的人因子IX負(fù)責(zé)將X因子激活至Xa。緩沖液組成：20mMTris-HCl/0.1MNaCl/1mMBenzamidine/pH7.4消光系數(shù)（1％）=13.2分子量=56,000道爾頓我們公司Zda優(yōu)勢是強(qiáng)大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時限加急費(fèi)全免4：價格公道，絕大部分價格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都是價格Zdi,因為價格Zdi意味著沒有服務(wù).5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們du家代理的品牌有：AntibodyResearchCorporation，arcticzymes，Biorelevant，AmberGen,Inc.，clemente-associates，clodronateliposomes，ColumbiaBiosciences，enzymeresearch，GeneBridgesGmbH，Genovis，AmberGen,Inc.BiotechnologyGmbH，HaematologicTechnologiesHTIHaemtech，hookelabs，Immudex，InnovativeResearchofAmerica，inspiralis，ListBiologicalLaboratories,Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-FreezBiologicals，pentapharm，progen，ProteinArk，QA-Bio,Inc，QA-Bio,IncQuickZymeBiosciences，Teknova，TriLinkBioTechnologies,Inc.，ZyagenLaboratories等7：我們還是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā)，歡迎合作。[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Recombinant Human F3 / Tissue factor / CD142

  RecombinantHumanF3/Tissuefactor/CD142CatalogNumber:13133-H02HGeneralInformationGeneNameSynonym:CD142,FLJ17960,TF,TFAProteinConstruction:ADNAsequenceencodingthehumanF3（P13726）extracellulardomain（Met1-Glu251）wasfusedwiththeFcregionofhumanIgG1attheC-terminus.Source:HumanExpressionHost:HumanCellQCTestingPurity:>92%asdeterminedbySDS-PAGE.Endotoxin:1.0EUperμgproteinasdeterminedbytheLALmethodStability:Samplesarestableforuptotwelvemonthsfromdateofreceiptat-70℃PredictedNterminal:Ser33MolecularMass:TherecombinanthumanF3/Fcisadisulfide-linkedhomodimericprotein.Thereducedmonomerconsistsof460aminoacidsandhasapredictedmolecularmassof51.8kDa.Itmigratesasanapproximately66kDabandinSDS-PAGEunderreducingconditionsduetoglycosylation.Formulation:LyophilizedfromsterilePBS,pH7.4Normally5%-8%trehaloseandmannitolareaddedasprotectantsbeforelyophilization.SpecificconcentrationsareincludedinthehardcopyofCOA.Pleasecontactusforanyconcernsorspecialrequirements.SDS-PAGE:UsageGuideStorage:Storeitundersterileconditionsat-70℃uponreceiving.Recommendtoaliquottheproteinintosmallerquantitiesforoptimalstorage.Avoidrepeatedfreeze-thawcycles.Reconstitution:Detailedreconstitutioninstructionsaresentalongwiththeproducts.ProteinDescriptionTissuefactor,alsoknownascoagulationfactorIII,F3,andCD142,isasingle-passtypeImembraneproteinwhichbelongstothetissuefactorfamily.Tissuefactorisoneoftheproteinsthatparticipateinhemostaticandinflammatoryprocesses.Activatedmonocytespresentintheliverincreaseexpressionoftissuefactor,andwhileaccumulatingintheorgantheycanintensifyinflammation.Tissuefactoristheproteinthatactivatesthebloodclottingsystembybindingto,andactivating,theplasmaserineprotease,factorVIIa,followingvascularinjury.Becauseofitsessentialroleinhemostasis,Tissuefactorplaysaroleinpathologyassociatedwithhemostasis,triggeringthecoagulationsysteminmanythromboticdiseasesandthecoagulopathiesassociatedwithsepsisandotherformsofdisseminatedintravascularcoagulation.Tissuefactorisnotonlythemainphysiologicalinitiatorofnormalbloodcoagulation,butisalsoimportantinthenaturalhistoryofsolidmalignanciesinthatitpotentiatesmetastasisandangiogenesisandmediatesoutside-insignal領(lǐng).Tissuefactorisexpressedconstitutivelybymanytissueswhicharenotincontactwithbloodandbyothercellsuponinjuryoractivation;thelatterincludeendothelialcells,tissuemacrophages,andperipheralbloodmonocytes.References1.Lwaleed,B.A.etal.,2001,JPathol.193（1）:3-12.2.Morrissey,JH.2004,IntJHematol.79（2）:103-8.3.Panasiuk,A.etal.,2007,BloodCoagulFibrinolysis.18（8）:739-44.4.Kinev,AV.etal.,2008,Lupus.17（10）:192211[詳細(xì)]

  2024-09-11 17:59

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Exosomes Released by Melanoma Cells Prepare Sentinel Lymph Nodes for Tumor Metastasis

  Exosomesarenaturallyoccurringbiologicalnanovesiclesutilizedbytumorstocommunicatesignalstolocalandremotecellsandtissues.Melanomaexosomescaninciteaproangiogenicsigna領(lǐng)programcapableofremode領(lǐng)tissuematrices.Inthisstudy,weshowexosome-mediatedconditioningoflymphnodesanddefinemicroanatomicresponsesthatlicensemetastasisofmelanomacells.Homingofmelanomaexosomestosentinellymphnodesimposessynchronizedmolecularsignalsthateffectmelanomacellrecruitment,extracellularmatrixdeposition,andvascularproliferationinthelymphnodes.Ourfindingshighlightthepathophysiologicroleandmechanismsofanexosome-mediatedprocessofmicroanatomicnichepreparationthatfacilitateslymphaticmetastasisbycancercells.[詳細(xì)]

  2018-08-31 10:11

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• 眼鏡蛇毒因子 （CVF）Cobra venom factor

  產(chǎn)品搜索請輸入產(chǎn)品關(guān)鍵字：產(chǎn)品分類品牌分類抗體一抗標(biāo)記一抗進(jìn)口一抗RD一抗CST一抗abcam一抗Elisa試劑盒人Elisa試劑盒大鼠Elisa試劑盒小鼠Elisa試劑盒牛Elisa試劑盒猴Elisa試劑盒鴨Elisa試劑盒魚Elisa試劑盒山羊Elisa試劑盒馬Elisa試劑盒其他Elisa試劑盒貓Elisa試劑盒裸鼠Elisa試劑盒倉鼠Elisa試劑盒綿羊Elisa試劑盒植物Elisa試劑盒兔Elisa試劑盒豚鼠Elisa試劑盒豬Elisa試劑盒雞Elisa試劑盒犬Elisa試劑盒abcam抗體細(xì)胞因子Elisa試劑盒小鼠細(xì)胞因子Elisa試劑盒invitrogenR&DElisa試劑盒小鼠Elisa試劑盒人Elisa試劑盒環(huán)保設(shè)備化學(xué)試劑指示劑PH緩沖液標(biāo)準(zhǔn)滴定溶液蛇毒試劑重組蛋白生化試劑其他標(biāo)準(zhǔn)品/對照品暫無信息聯(lián)系方式地址：上海閔行紫竹科技園郵編：201109聯(lián)系人：茅經(jīng)理電話：021-60513351傳真：021-34668965手機(jī)：18049719720留言：發(fā)送留言個性化：www.shkbsw.com網(wǎng)址：展臺：192212/友情連接ZG化工儀器網(wǎng)資料下載磷酸二酯酶Phosphodiesterase蛇毒試劑（質(zhì)量標(biāo)準(zhǔn)）Z近更新時間：2012-08-08提供商：上海凱博生化試劑有限公司資料大?。?9KB文件類型：WORD文檔下載次數(shù)：0次資料類型：瀏覽次數(shù)：1次相關(guān)產(chǎn)品：[詳細(xì)]

  2018-09-04 10:00

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Bovine Fibrinogen, Plasminogen Depleted （Factor I） 說明書

  EnzymeResearch，EnzymeResearch介紹,EnzymeResearch代理,EnzymeResearch總代,EnzymeResearch**代理,EnzymeResearchZG代理EnzymeResearch是一家擁有超過25年的酶研究的實驗室，生產(chǎn)和開發(fā)多種用于基本凝血研究的多種酶和輔因子。在過去的十年EnzymeResearchLaboratories為科學(xué)家參與止血和血栓形成的研究提供了寶貴支持。EnzymeResearch通過不斷追求產(chǎn)品的Z高品質(zhì)，贏得了世界各地凝血研究實驗室的信任。EnzymeResearchLaboratories為生物技術(shù)公司,制藥行業(yè)、醫(yī)院和大學(xué)的研究實驗室提供用于全方位的純化抗凝因子。除了其制造能力，EnzymeResearchLaboratories也創(chuàng)新研發(fā)領(lǐng)域中的血液凝固技術(shù)。BovineFibrinogen,PlasminogenDepleted（FactorI）BFIB1BovineFibrinogen,PlasminogenDepleted（FactorI）Purifiedfromfreshlycollectedbovineplasma.TheproteinpurityisdeterminedbySDS-PAGEandisgreaterthan95%clottable.Conversionofsolublefibrinogentotheinsolubleclot-formingfibrinistheterminalstageofcoagulation.Buffercomposition=20mMSodiumCitrate-HCL/pH7.4*ExtinctionCoefficient（1%）=15.1MolecularWeight=330,000daltons上海起福生物科技有限公司聯(lián)系人：楊建輝400電話：4006551678辦公電話：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號1102室[詳細(xì)]

  2018-09-29 10:03

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Bovine Factor XIa（牛凝血酶因子XIa）

  EnzymeResearch，EnzymeResearch介紹,EnzymeResearch代理,EnzymeResearch總代,EnzymeResearch**代理,EnzymeResearchZG代理EnzymeResearch是一家擁有超過25年的酶研究的實驗室，生產(chǎn)和開發(fā)多種用于基本凝血研究的多種酶和輔因子。在過去的十年EnzymeResearchLaboratories為科學(xué)家參與止血和血栓形成的研究提供了寶貴支持。EnzymeResearch通過不斷追求產(chǎn)品的Z高品質(zhì)，贏得了世界各地凝血研究實驗室的信任。EnzymeResearchLaboratories為生物技術(shù)公司,制藥行業(yè)、醫(yī)院和大學(xué)的研究實驗室提供用于全方位的純化抗凝因子。除了其制造能力，EnzymeResearchLaboratories也創(chuàng)新研發(fā)領(lǐng)域中的血液凝固技術(shù)。BovineFactorXIaBFXIa11aBovineFactorXIaBovineFactorXlaispurifiedfromfreshlycollectedBovinePlasmausingacombinationofsaltprecipitationsandactivationonanegativesurface.ThisFactorXlaisapotentactivatorofbothHumanandBovineFactorIX.BovineFactorXIapurityisdeterminedbySDS-PAGEandshowscompletereductionuponincubationwith2-mercaptoethanol.Buffercomposition=50mMTris-HCl/0.1MNaCl/pH8.0ProteinconcentrationisdeterminedbyBCA上海起福生物科技有限公司聯(lián)系人：楊建輝400電話：4006551678辦公電話：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號1102室[詳細(xì)]

  2018-09-29 10:03

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• Platelet Factor 4（血小板因子4）說明書

  EnzymeResearch，EnzymeResearch介紹,EnzymeResearch代理,EnzymeResearch總代,EnzymeResearch**代理,EnzymeResearchZG代理EnzymeResearch是一家擁有超過25年的酶研究的實驗室，生產(chǎn)和開發(fā)多種用于基本凝血研究的多種酶和輔因子。在過去的十年EnzymeResearchLaboratories為科學(xué)家參與止血和血栓形成的研究提供了寶貴支持。EnzymeResearch通過不斷追求產(chǎn)品的Z高品質(zhì)，贏得了世界各地凝血研究實驗室的信任。EnzymeResearchLaboratories為生物技術(shù)公司,制藥行業(yè)、醫(yī)院和大學(xué)的研究實驗室提供用于全方位的純化抗凝因子。除了其制造能力，EnzymeResearchLaboratories也創(chuàng)新研發(fā)領(lǐng)域中的血液凝固技術(shù)。PlateletFactor4（PF4）PF4PlateletFactor4PlateletFactor4ispurifiedfromHumanplateletsbyaffinitychromatographyandgelfiltration.Ithasamolecularweightof29,000daltonsandismadeupof4identicalsubunitsof7,800daltonseach.Buffercomposition=50mMTris-HCl/1.5MNaCl/0.02%NaN3/pH7.3ExtinctionCoefficient（1%）=2.6MolecularWeight=29,000daltons上海起福生物科技有限公司聯(lián)系人：楊建輝400電話：4006551678辦公電話：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號1102室[詳細(xì)]

  2018-09-29 10:03

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• 人類凝血因子（Human Factor VIIa） HFVIIa說明書

  EnzymeResearch是一家擁有超過25年的酶研究的實驗室，生產(chǎn)和開發(fā)多種用于基本凝血研究的多種酶和輔因子。在過去的十年EnzymeResearchLaboratories為科學(xué)家參與止血和血栓形成的研究提供了寶貴支持。EnzymeResearch通過不斷追求產(chǎn)品的Z高品質(zhì)，贏得了世界各地凝血研究實驗室的信任。HumanFactorVIIaHFVIIaHumanFactorVIIaPreparedfrompurifiedHumanFactorVIIusingHumanFactorXIIa.TheFactorXllaisremovedusingaffinitychromatography.PurityisdeterminedbySDS-PAGE.HumanFactorVIIareducesto29,500and23,500withtheadditionof2-mercaptoethanol.Activityisdeterminedviaclottingassay.FactorVlla,inthepresenceofcalciumionsandTissuefactor,activatesFactorsIXandXtotheirenzymaticallyactiveforms,FactorIXaandXa.Buffercomposition=20mMTris-HCl/0.1MNaCl/pH7.4*ExtinctionCoefficient（1%）=13.9MolecularWeight=50,000daltonsAlsoavailable:des-Gla-HumanFactorVIIaandFactorVIIainactivated上海起福生物科技有限公司聯(lián)系人：楊建輝400電話：4006551678辦公電話：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號1102室[詳細(xì)]

  2018-09-29 10:03

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  • 

• 人纖維蛋白原Human Fibrinogen 3（Factor I）說明書

  EnzymeResearch，EnzymeResearch介紹,EnzymeResearch代理,EnzymeResearch總代,EnzymeResearch**代理,EnzymeResearchZG代理EnzymeResearch是一家擁有超過25年的酶研究的實驗室，生產(chǎn)和開發(fā)多種用于基本凝血研究的多種酶和輔因子。在過去的十年EnzymeResearchLaboratories為科學(xué)家參與止血和血栓形成的研究提供了寶貴支持。HumanFibrinogen（FactorI）HumanFibrinogenFIB3Plasminogen,vonWillebrandFactorandFibronectinDepletedConversionofsolublefibrinogentotheinsolublefibrinisthefinalstepinthebloodcoagulationcascade.ERLoffersthreetypesofpurifiedfibrinogenpreparedbyacombinationofsaltprecipitation,affinitychromatographyandimmunoaffinitychromatographymethodstosuittheindividualresearcher'sneeds.Allthreeformsare>95%clottable（byfunctionalassays）andpurityis>95%bySDS-PAGE.FIB1PlasminogenDepletedFIB2PlasminogenandvonWillebrandFactorDepletedFIB3Plasminogen,vonWillebrandFactorandFibronectinDepletedBuffercomposition=20mMSodiumCitrate-HCl/pH7.4*ExtinctionCoefficient（1%）=15.1MolecularWeight=330,000daltons上海起福生物科技有限公司聯(lián)系人：楊建輝400電話：4006551678辦公電話：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號1102室[詳細(xì)]

  2018-09-29 10:03

  產(chǎn)品樣冊

  |

  • 
  • 
  • 
  •