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• 斑馬魚卵黃蛋白原ELISA KIT

  斑馬魚卵黃蛋白原ELISA KIT[詳細]

  2024-09-28 00:19

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• 魚卵黃蛋白原（VTG）ELISA試劑盒說明書

  FishVTGELISAKitForthequantitativeinvitrodeterminationofFishVitellogeninconcentrationsinbodyfluid-celiacfluid-tissuehomogenates-otherbiologicalfluidsFORLABORATORYRESEARCHUSEONLY.NOTFORUSEINDIAGNOSTICPROCEDURES.Thispackageinsertmustbereadinitsentiretybeforeusingthisproduct.ELISAENZYMELINKEDIMMUNOSORBENTASSAYINTENDEDUSEANDTESTPRINCIPLEThisVTGELISAkitisintendedLaboratoryforResearchuseonlyandisnotforuseindiagnosticortherapeuticprocedures.TheStopSolutionchangesthecolorfrombluetoyellowandtheintensityofthecolorismeasuredat450nmusingaspectrophotometer.InordertomeasuretheconcentrationofVTGinthesample,thisVTGELISAKitincludesasetofcalibrationstandards.ThecalibrationstandardsareassayedatthesametimeasthesamplesandallowtheoperatortoproduceastandardcurveofOpticalDensityversusVTGconcentration.TheconcentrationofVTGinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.SAMPLECOLLECTIONANDSTORAGESSerum-Useaserumseparatortubeandallowsamplestoclotfortwohoursatroomtemperatureorovernightat4℃beforecentrifugationfor20minutesatapproximately1000×g.Assayfreshlypreparedserumimmediatelyorstoresamplesinaliquotat-20℃or-80℃forlateruse.Avoidrepeatedfreeze/thawcycles.Plasma-CollectplasmausingEDTAorheparinasananticoagulant.Centrifugesamplesfor15minutesat1000×gat2-8℃within30minutesofcollection.Removeplasmaandassayimmediatelyorstoresamplesinaliquotat-20℃or-80℃forlateruse.Avoidrepeatedfreeze/thawcycles.Tissuehomogenates-Forgeneralinformation,hemolysisbloodmayaffecttheresult,soyou首ldrinsethetissueswithice-coldPBS(0.01M,pH=7.4)toremoveexcessbloodthoroughly.Tissuepieces首ldbeweighedandthenmincedtosmallpieceswhichwillbehomogenizedinPBS(thevolumedependsontheweightofthetissue.9mLPBSwouldbeappropriateto1gramtissuepieces.SomeproteaseinhibitorisrecommendedtoaddintothePBS.)withaglasshomogenizeronice.Tofurtherbreakthecells,youcansonicatethesuspensionwithanultrasoniccelldisrupterorsubjectittofreeze-thawcycles.Thehomogenatesarethencentrifugatedfor5minutesat5000×gtogetthesupernate.Cellculturesupernatesandotherbiologicalfluids-Centrifugesamplesfor20minutesat1000×g.Removeparticulatesandassayimmediatelyorstoresamplesinaliquotat-20℃or-80℃forlateruse.Avoidrepeatedfreeze/thawcycles.Note:Thesamples首lebecentrifugateddequatelyandnohemolysisorgranulewasallowed.MATERIALSREQUIREDBUTNOTSUPPLIED1.37℃incubator2.Standardmicroplatereadercapableofmeasuringabsorbanceat450nm3.Precisionpipettes,disposablepipettetipsandAbsorbentpaper4.DistilledordeionizedwaterREAGENTSPROVIDEDAllreagentsprovidedarestoredat2-8°C.Refertotheexpirationdateonthelabel.Name96determinations48determinationsMICROTITERPLATE8*12strips8*6stripsSTANDARD（6vial）0.3ml/vial0.3ml/vialSAMPLEDILUENT6.0ml3.0mlENZYMECONJUGATE10.0ml5.0mlWASHSOLUTION25ml15mlSUBSTRATEA6.0ml3.0mlSUBSTRATEB6.0ml3.0mlSTOPSOLUTION6.0ml3.0mlClosureplatemembrane22Usermanual11Sealedbags11Note:1.Standardconcentrationwasfollowedby:480,240,120,60,30,15ng/mL.2.Ifsamplesgeneratevalueshigherthanthehigheststandard,pleasedilutethesampleswithSampleDiluentandrepeattheassay.PRECAUTIONSDonotsubstitutereagentsfromonekitlottoanother.Standard,conjugateandmicrotiterplatesarematchedforoptimalperformance.Useonlythereagentssuppliedbymanufacturer.Allowkitreagentsandmaterialstoreachroomtemperature(20-25°C)beforeuse.Donotusewaterbathstothawsamplesorreagents.Donotusekitcomponentsbeyondtheirexpirationdate.Useonlydeionizedordistilledwatertodilutereagents.Donotremovemicrotiterplatefromthestoragebaguntilneeded.Unusedstrips首ldbestoredat2-8°Cintheirpouchwiththedesiccantprovided.Usefreshdisposablepipettetipsforeachtransfertoavoidcontamination.Donotmixacidandsodiumhypochloritesolutions.Serumandplasma首ldbehandledaspotentiallyhazardousandcapableoftransmittingdisease.Disposableglovesmustbewornduringtheassayprocedure,sincenoknowntestmethodcanoffercompleteassurancethatproductsderivedfromRatbloodwillnottransmitinfectiousagents.Therefore,allbloodderivatives首ldbeconsideredpotentiallyinfectiousandgoodlaboratorypractices首ldbefollowed.Allsamples首ldbedisposedofinamannerthatwillinactivateviruses.LiquidWaste:Addsodiumhypochloritetoafinalconcentrationof1.0%.Thewaste首ldbeallowedtostandforaminimumof30minutestoinactivatethevirusesbeforedisposal.SubstrateSolutioniseasilycontaminated.Ifbluishpriortouse,donotuse.SubstrateBcontain20%acetone,keepthisreagentawayfromsourcesofheatorflame.Removeallkitreagentsfromrefrigeratorandallowthemtoreachroomtemperature(20-25°C).REAGENTPREPARATIONANDSTORAGEWashSolution(1X)-Dilute1volumeofWashsolution(20X)with19volumesofdeionizedordistilledwater.WashSolutionisstablefor1monthat2-8°C.ASSAYPROCEDURE1.Prepareallreagentsbeforestartingassayprocedure.ItisrecommendedthatallStandardsandSamplesbeaddedinduplicatetotheMicrotiterplate.2.Add50μlofStandardorSampletotheappropriatewells.Blankwelldoesn’taddanyting.3.Add100μlofEnzymeconjugatetostandardwellsandsamplewellsexcepttheblankwell,coverwithanadhesivestripandincubatefor60minutesat37°C.4.WashtheMicrotiterPlate4times.ManualWashing-Removeincubationmixturebyaspiratingcontentsoftheplateintoasinkorproperwastecontainer.Usingasquirtbottle,filleachwellcompletelywithWashSolution(1X),thenaspiratecontentsoftheplateintoasinkorproperwastecontainer.Repeatthisprocedureforatotaloffourtimes.Afterfinalwash,invertplate,andblotdrybyhittingplateontoabsorbentpaperorpapertowelsuntilnomoistureappears.Note:Holdthesidesoftheplateframefirmlywhenwashingtheplatetoassurethatallstripsremainsecurelyinframe.AutomatedWashing-Aspirateallwells,thenwashplatesfourtimesusingWashBuffer(1X).Alwaysadjustyourwashertoaspirateasmuchliquidaspossibleandsetfillvolumeat350μL/well/wash.Afterfinalwash,invertplate,andblotdrybyhittingplateontoabsorbentpaperorpapertowelsuntilnomoistureappears.5.AddSubstrateA50μlandSubstrateB50μltoeachwell.Gentlymixandincubatefor15minutesat37°C.Protectfromlight.6.Add50μlStopSolutiontoeachwell.Thecolorinthewells首ldchangefrombluetoyellow.Ifthecolorinthewellsisgreenorthecolorchangedoesnotappearuniform,gentlytaptheplatetoensurethoroughmixing.7.ReadtheOpticalDensity(O.D.)at450nmusingamicrotiterplatereaderwithin15minutes.CALCULATIONOFRESULTSThisstandardcurveisusedtodeterminetheamountinanunknownsample.ThestandardcurveisgeneratedbyplottingtheaverageO.D.(450nm)obtainedforeachofthesixstandardconcentrationsonthevertical(X)axisversusthecorrespondingconcentrationonthehorizontal(Y)axis.First,calculatethemeanO.D.valueforeachstandardandsample.AllO.D.Valuesaresubtractedbythemeanvalueofthebalnkwellbeforeresultinterpretation.Constructthestandardcurveusinggraphpaperorstatisticalsoftware.Todeterminetheamountineachsample,firstlocatetheO.D.valueontheY-axisandextendahorizontallinetothestandardcurve.Atthepointofintersection,drawaverticallinetotheX-axisandreadthecorrespondingconcentration.Anyvariationinoperator,pipettingandwashingtechnique,incubationtimeortemperature,andkitagecancausevariationinresult.Eachuser首ldobtaintheirownstandardcurve.Intra-assayCV(%)islessthan10%andInter-assayCV(%)islessthan15%.Assayrange:15ng/mL480ng/mL.7.Sensitivity:TheminimumdetectabledoseofFishVTGistypicallylessthan1.0ng/mL.8.Cross-reactivity:ThisassayrecognizesrecombinantandnaturalFishVTG.Nosignificantcross-reactivityorinterferencewasobserved.9.Storage:2-8℃(Usefrequently);sixmonths(-20℃)。10.StandardcurveFORRESEARCHUSEONLY;NOTFORTHERAPEUTICORDIAGNOSTICAPPLICATIONS!PLEASEREADTHROUGHENTIREPROCEDUREBEFOREBEGINNING!魚卵黃蛋白原（VTG）試劑盒（ELISA）使用說明書l本試劑盒用于體外定量檢測體液、腔液、組織勻漿及相關液體樣本中魚卵黃蛋白原（VTG）的含量。l有效期：6個月l保存條件：2-8℃實驗原理試劑盒采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗（ELISA）。往預先包被魚卵黃蛋白原（VTG）捕獲抗體的包被微孔中，依次加入標本、標準品、HRP標記的檢測抗體，經(jīng)過溫育并徹底洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成Z終的黃色。顏色的深淺和樣品中的魚卵黃蛋白原（VTG）呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），計算樣品濃度。樣本處理及要求1.血清：將收集于血清分離管的全血標本在室溫放置2小時或4℃過夜，然后1000×g離心20分鐘，取上清即可，或將上清置于-20℃或-80℃保存，但應避免反復凍融。2.血漿：用EDTA或肝素作為抗凝劑采集標本，并將標本在采集后的30分鐘內(nèi)于2-8℃1000×g離心15分鐘，取上清即可檢測，或將上清置于-20℃或-80℃保存，但應避免反復凍融。3.組織勻漿：用預冷的PBS(0.01M,pH=7.4)沖洗組織，去除殘留血液（勻漿中裂解的紅細胞會影響測量結果），稱重后將組織剪碎。將剪碎的組織與對應體積的PBS（一般按1:9的重量體積比，比如1g的組織樣品對應9mL的PBS，具體體積可根據(jù)實驗需要適當調(diào)整，并做好記錄。推薦在PBS中加入蛋白酶YZ劑）加入玻璃勻漿器中，于冰上充分研磨。為了進一步裂解組織細胞，可以對勻漿液進行超聲破碎，或反復凍融。Z后將勻漿液于5000×g離心5~10分鐘，取上清檢測。4.細胞培養(yǎng)物上清或其它生物標本：請1000×g離心20分鐘，取上清即可檢測，或將上清置于-20℃或-80℃保存，但應避免反復凍融。注：標本溶血會影響Z后檢測結果，因此溶血標本不宜進行此項檢測。需要而未提供的試劑和器材酶標儀（450nm）高精度加樣器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL37℃恒溫箱蒸餾水或去離子水試劑盒組成名稱96孔配置48孔配置備注微孔酶標板8孔×12條8孔×6條無標準品0.3mL*6管0.3mL*6管無樣本稀釋液6mL3mL無檢測抗體-HRP10mL5mL無20×洗滌緩沖液25mL15mL按說明書進行稀釋底物A6mL3mL無底物B6mL3mL無終止液6mL3mL無封板膜2張2張無說明書1份1份無自封袋1個1個無備注：1.標準品濃度依次為：480、240、120、60、30、15ng/mL2.經(jīng)過大量正常標本檢驗，標本的正常濃度值均在試劑盒提供的檢測范圍內(nèi)，實驗過程中直接取50μL樣本上樣即可。當有部分樣本值超過Zda標準品濃度時，可用樣本稀釋液將標本進行適當稀釋后再進行實驗。注意事項嚴格按照規(guī)定的時間和溫度進行溫育以保證準確結果。所有試劑都必須在使用前達到室溫20-25℃。使用后立即冷藏保存試劑。洗板不正確可以導致不準確的結果。在加入底物前確保盡量吸干孔內(nèi)液體。溫育過程中不要讓微孔干燥掉。消除板底殘留的液體和手指印，否則影響OD值。底物顯色液應呈無色或很淺的顏色，已經(jīng)變藍的底物液不能使用。避免試劑和標本的交叉污染以免造成錯誤結果。在儲存和溫育時避免強光直接照射。平衡至室溫后再打開密封袋以防水滴凝聚在冷板條上。任何反應試劑不能接觸漂白溶劑或漂白溶劑所散發(fā)的強烈氣體。任何漂白成分都會破壞試劑盒中反應試劑的生物活性。不能使用過期產(chǎn)品。如果可能傳播疾病，所有的樣品都應管理好，按照規(guī)定的程序處理樣品和檢測裝置。試劑準備試劑盒從冷藏環(huán)境中取出應在室溫平衡后方可使用。20×洗滌緩沖液的稀釋：蒸餾水按1：20稀釋，即1份20×洗滌緩沖液加19份蒸餾水。操作步驟從室溫平衡20min后的鋁箔袋中取出所需板條，剩余板條用自封袋密封放回4℃。設置標準品孔和樣本孔，標準品孔各加不同濃度的標準品50μL；樣本孔中加入待測樣本50μL；空白孔不加。除空白孔外，標準品孔和樣本孔中每孔加入辣根過氧化物酶（HRP）標記的檢測抗體100μL，用封板膜封住反應孔，37℃水浴鍋或恒溫箱溫育60min。棄去液體，吸水紙上拍干，每孔加滿洗滌液（350μL），靜置1min，甩去洗滌液，吸水紙上拍干，如此重復洗板5次（也可用洗板機洗板）。每孔加入底物A、B各50μL，37℃避光孵育15min。每孔加入終止液50μL，15min內(nèi)，在450nm波長處測定各孔的OD值。實驗結果計算以所測標準品的OD值為橫坐標，標準品的濃度值為縱坐標，在坐標紙上或用相關軟件繪制標準曲線，并得到直線回歸方程，將樣品的OD值代入方程，計算出樣品的濃度。試劑盒性能檢測范圍：15ng/mL480ng/mL。靈敏度：Zdi檢測濃度小于1.0ng/mL。特異性：不與其它可溶性結構類似物交叉反應。重復性：板內(nèi)變異系數(shù)小于10%，板間變異系數(shù)小于15%。[詳細]

  2018-12-11 10:00

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• 魚卵黃蛋白原（VTG）酶聯(lián)免疫分析試劑盒使用說明書

  魚卵黃蛋白原（VTG）酶聯(lián)免疫分析試劑盒使用說明書本試劑盒僅供研究使用。檢測范圍：96T30μg/L-850μg/L使用目的：本試劑盒用于測定魚血清、血漿及相關液體樣本中卵黃蛋白原（VTG）含量。實驗原理本試劑盒應用雙抗體夾心法測定標本中魚卵黃蛋白原（VTG）水平。用純化的魚卵黃蛋白原（VTG）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入卵黃蛋白原（VTG），再與HRP標記的卵黃蛋白原（VTG）抗體結合，形成抗體-抗原-酶標抗體復合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，并在酸的作用下轉化成Z終的黃色。顏色的深淺和樣品中的卵黃蛋白原（VTG）呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中魚卵黃蛋白原（VTG）濃度。魚卵黃蛋白原（VTG）酶聯(lián)免疫分析試劑盒組成標本要求1．標本采集后盡早進行提取，提取按相關文獻進行，提取后應盡快進行實驗。若不能馬上進行試驗，可將標本放于-20℃保存，但應避免反復凍融2．不能檢測含NaN3的樣品，因NaN3YZ辣根過氧化物酶的（HRP）活性。操作步驟1.標準品的稀釋：本試劑盒提供原倍標準品一支，用戶可按照下列圖表在小試管中進行稀釋。2.加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑，其余各步操作相同）、標準孔、待測樣品孔。在酶標包被板上標準品準確加樣50μl，待測樣品孔中先加樣品稀釋液40μl，然后再加待測樣品10μl（樣品Z終稀釋度為5倍）。加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻。3.溫育：用封板膜封板后置37℃溫育30分鐘。4.配液：將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用5.洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復5次，拍干。6.加酶：每孔加入酶標試劑50μl，空白孔除外。7.溫育：操作同3。8.洗滌：操作同5。9.顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘.10.終止：每孔加終止液50μl，終止反應（此時藍色立轉黃色）。11.測定：以空白空調(diào)零，450nm波長依序測量各孔的吸光度（OD值）。測定應在加終止液后15分鐘以內(nèi)進行。魚卵黃蛋白原（VTG）酶聯(lián)免疫分析試劑盒操作程序總結：計算以標準物的濃度為橫坐標，OD值為縱坐標，在坐標紙上繪出標準曲線，根據(jù)樣品的OD值由標準曲線查出相應的濃度；再乘以稀釋倍數(shù)；或用標準物的濃度與OD值計算出標準曲線的直線回歸方程式，將樣品的OD值代入方程式，計算出樣品濃度，再乘以稀釋倍數(shù)，即為樣品的實際濃度。注意事項1．試劑盒從冷藏環(huán)境中取出應在室溫平衡15-30分鐘后方可使用，酶標包被板開封后如未用完，板條應裝入密封袋中保存。2．濃洗滌液可能會有結晶析出，稀釋時可在水浴中加溫助溶，洗滌時不影響結果。3．各步加樣均應使用加樣器，并經(jīng)常校對其準確性，以避免試驗誤差。一次加樣時間**控制在5分鐘內(nèi)，如標本數(shù)量多，推薦使用排槍加樣。4．請每次測定的同時做標準曲線，**做復孔。如標本中待測物質含量過高（樣本OD值大于標準品孔**孔的OD值），請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定，計算時請Z后乘以總稀釋倍數(shù)（×n×5）。5．封板膜只限一次性使用，以避免交叉污染。6．底物請避光保存。7．嚴格按照說明書的操作進行，試驗結果判定必須以酶標儀讀數(shù)為準.8．所有樣品，洗滌液和各種廢棄物都應按傳染物處理。9．本試劑不同批號組分不得混用。魚卵黃蛋白原（VTG）酶聯(lián)免疫分析試劑盒保存條件及有效期1．試劑盒保存：2-8℃。2．有效期：6個月[詳細]

  2018-11-04 10:00

  產(chǎn)品樣冊

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• 鯉魚卵黃蛋白原ELISA檢測試劑盒

  鯉魚卵黃蛋白原ELISA檢測試劑盒[詳細]

  2024-10-05 20:57

  應用文章

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• 豬血纖蛋白原(Fbg)ELISA試劑盒

  豬血纖蛋白原(Fbg)ELISA試劑盒[詳細]

  2024-09-29 04:29

  操作手冊

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• Monkey ELISA Kit

  MonkeyELISAKit[詳細]

  2024-09-28 07:04

  產(chǎn)品樣冊

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• 小鼠血纖蛋白原(Fbg)ELISA試劑盒

  小鼠血纖蛋白原(Fbg)ELISA試劑盒[詳細]

  2013-12-09 00:00

  期刊論文

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• 大鼠血纖蛋白原(Fbg)ELISA試劑盒

  大鼠血纖蛋白原(Fbg)ELISA試劑盒[詳細]

  2024-09-28 00:34

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• 兔血纖蛋白原(Fbg)ELISA試劑盒

  兔血纖蛋白原(Fbg)ELISA試劑盒[詳細]

  2024-09-29 04:17

  課件

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• 人凝血酶ELISA Kit

  人凝血酶ELISA Kit[詳細]

  2014-08-21 00:00

  安裝說明

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• Human IL-22BP ELISA kit

  Human IL-22BP ELISA kit[詳細]

  2015-10-09 00:00

  產(chǎn)品樣冊

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• Human PLA2R1 ELISA kit

  Human PLA2R1 ELISA kit[詳細]

  2015-10-09 00:00

  應用文章

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• Mouse CaSR ELISA kit

  Mouse CaSR ELISA kit[詳細]

  2015-10-09 00:00

  操作手冊

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• Human AMH ELISA kit

  Human AMH ELISA kit[詳細]

  2015-10-09 00:00

  其它

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• Rat Aβ1-42 ELISA kit

  www.biokanu.comRatamyloidbetapeptide1-42（Aβ1-42）ELISAKitProd.No.3R285FROM:RBForthequantitativeinvitrodeterminationofAβ1-42concentrationsinRatsupernates,serum,plasmaandtissue.FORRESEARCHUSEONLY.NOTFORUSEINDIAGNOSTICPROCEDURES.TABLEOFCONTENTSContentsPageTABLEOFCONTENTS..2INTENDEDUSE..3PRINCIPLE..3WARNINGSANDPRECAUTIONS..4MATERIALSPROVIDEDWITHTHEKIT.7MATERIALSREQUIREDBUTNOTPROVIDED..7STORAGECONDITIONS..8REAGENTPREPARATION..9SPECIMENCOLLECTIONANDPREPARATION..9ASSAYPROCEDURE..10CALCULATIONOFRESULTS..13REFERENCES..14INTENDEDUSEAnenzymeimmunoassayforthequantitativeinvitrodiagnosticmeasurementofRatAβ1-42incellculturesupernates,serum,plasmaandtissue.PRINCIPLEThekitassayRatAβ1-42levelinthesample，usePurifiedRatAβ1-42antibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddAβ1-42towells,CombinedAβ1-42antibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofAβ1-42inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.WARNINGSANDPRECAUTIONSlThiskitisforinvitrodiagnosticuseonly.Forprofessionaluseonly.lAllreagentsofthistestkitwhichcontainhumanserumorplasmahavebeentestedandconfirmednegativeforHIVI/II,HBsAgandHCVbyFDAapprovedprocedures.Allreagents,however,首ldbetreatedaspotentialbiohazardsinuseandfordisposal.lBeforestartingtheassay,readtheinstructionscompletelyandcarefully.Usethevalidversionofthepackageinsertprovidedwiththekit.Besurethateverythingisunderstood.lThemicroplatecontainssnap-offstrips.Unusedwellsmustbestoredat2°Cto8°Cinthesealedfoilpouchandusedintheframeprovided.lPipettingofsamplesandreagentsmustbedoneasquicklyaspossibleandinthesamesequenceforeachstep.lUsereservoirsonlyforsinglereagents.Thisespeciallyappliestothesubstratereservoirs.Usingareservoirfordispensingasubstratesolutionthathadpreviouslybeenusedfortheconjugatesolutionmayturnsolutioncolored.Donotpourreagentsbackintovialsasreagentcontaminationmayoccur.lMixthecontentsofthemicroplatewellsthoroughlytoensuregoodtestresults.Donotreusemicrowells.lDonotletwellsdryduringassay;addreagentsimmediatelyaftercompletingtherinsingsteps.lAllowthereagentstoreachroomtemperature（21-26°C）beforestartingthetest.Temperaturewillaffecttheabsorbancereadingsoftheassay.However,valuesforthepatientsampleswillnotbeaffected.lNeverpipetbymouthandavoidcontactofreagentsandspecimenswithskinandmucousmembranes.lDonotsmoke,eat,drinkorapplycosmeticsinareaswherespecimensorkitreagentsarehandled.lWeardisposablelatexgloveswhenhand領specimensandreagents.Microbialcontaminationofreagentsorspecimensmaygivefalseresults.lHand領首ldbedoneinaccordancewiththeproceduresdefinedbyanappropriatenationalbiohazardsafetyguidelineorregulation.lDonotusereagentsbeyondexpirydateasshownonthekitlabels.lAllindicatedvolumeshavetobeperformedaccordingtotheprotocol.Optimaltestresultsareonlyobtainedwhenusingcalibratedpipettesandmicrotiterplatereaders.lDonotmixorusecomponentsfromkitswithdifferentlotnumbers.Itisadvisednottoexchangewellsofdifferentplatesevenofthesamelot.Thekitsmayhavebeenshippedorstoredunderdifferentconditionsandthebindingcharacteristicsoftheplatesmayresultslightlydifferent.lAvoidcontactwithStopSolutioncontaining0.5MH2SO4.Itmaycauseskinirritationandburns.lSomereagentscontainProclin,BNDand/orMITaspreservatives.Incaseofcontactwitheyesorskin,flushimmediatelywithwater.lTMBsubstratehasanirritanteffectonskinandmucosa.Incaseofpossiblecontact,washeyeswithanabundantvolumeofwaterandskinwithsoapandabundantwater.Washcontaminatedobjectsbeforereusingthem.Ifinhaled,takethepersontoopenair.lChemicalsandpreparedorusedreagentshavetobetreatedashazardouswasteaccordingtothenationalbiohazardsafetyguidelineorregulation.lForinformationonhazardoussubstancesincludedinthekitpleaserefertoMaterialSafetyDataSheetsMATERIALSPROVIDEDWITHTHEKITMaterialsprovidedwiththekit96determinationsStorageUsermanual1Closureplatemembrane2Sealedbags1Microelisastripplate12-8℃Standard：900pg/ml0.5ml×1bottle2-8℃Standarddiluent1.5ml×1bottle2-8℃HRP-Conjugatereagent6ml×1bottle2-8℃Samplediluent6ml×1bottle2-8℃ChromogenSolutionA6ml×1bottle2-8℃ChromogenSolutionB6ml×1bottle2-8℃StopSolution6ml×1bottle2-8℃washsolution30×20ml×1bottle2-8℃MATERIALSREQUIREDBUTNOTPROVIDEDlMicroplatereadercapableofmeasuringabsorbanceat450nm.lPrecisionpipettestodeliver2mlto1mlvolumes.l100mland1litergraduatedcylinders.lCalibratedadjustableprecisionpipettes,preferablywithdisposableplastictips.（Amanifoldmulti-channelpipetteisdesirableforlargeassays.）lAbsorbentpaper.l37°Cincubator.lDistilledordeionizedwater.lDataanalysisandgraphingsoftware.Graphpaper:linear（Cartesian）,log-logorsemi-log,orlog-logitasdesired.lTubestopreparestandardorsampledilutions.STORAGECONDITIONSuWhenstoredat2°Cto8°Cunopenedreagentswillretainreactivityuntilexpirationdate.uDonotusereagentsbeyondthisdate.Openedreagentsmustbestoredat2°Cto8°C.uMicrotiterwellsmustbestoredat2°Cto8°C.Oncethefoilbaghasbeenopened,care首ldbetakentocloseittightlyagain.uOpenedkitsretainactivityfor8weeksifstoredasdescribedabove.REAGENTPREPARATIONBringallreagentstoroomtemperaturebeforeuseSPECIMENCOLLECTIONANDPREPARATIONSerum-Useaserumseparatortube（SST）andallowsamplestoclotfor30minutesbeforecentrifugationfor15minutesatapproximately1000xg.Removeserumandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Plasma-CollectplasmausingEDTAorheparinasananticoagulant.Centrifugesamplesfor15minutesat1000xgat2-8°Cwithin30minutesofcollection.Storesamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcycles.Cellculturefluidandotherbiologicalfluids-Removeparticulatesbycentrifugationandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcyclesASSAYPROCEDUREuGeneralRemarkslAllreagentsandspecimensmustbeallowedtocometoroomtemperaturebeforeuse.Allreagentsmustbemixedwithoutfoaming.lOncethetesthasbeenstarted,allsteps首ldbecompletedwithoutinterruption.lUsenewdisposalplasticpipettetipsforeachstandard,controlorsampleinordertoavoidcrosscontamination.lAbsorbanceisafunctionoftheincubationtimeandtemperature.Beforestartingtheassay,itisrecommendedthatallreagentsareready,capsremoved,allneededwellssecuredinholder,etc.Thiswillensureequalelapsedtimeforeachpipettingstepwithoutinterruption.lAsageneralruletheenzymaticreactionislinearlyproportionaltotimeandtemperature.lDetermineabsorptionwithanELISAreaderat450nmagainst620nmasreference.Ifnoreferencewavelengthisavailable,readonlyat450nm.Iftheextinctionofthehigheststandardexceedsthemeasurementrangeofthephotometer,absorptionmustbemeasuredimmediatelyat405nmagainst620nmasreference.uAssayProcedure1.DiluteandaddsampletoStandard:set10StandardwellsontheELISAplatescoated,addStandard100μltothefirstandthesecondwell,thenaddStandarddilution50μltothefirstandthesecondwell,mix;takeout100μlformthefirstandthesecondwellthenaddittothethirdandtheforthwellseparately.thenaddStandarddilution50μltothethirdandtheforthwell,mix;thentakeout50μlfromthethirdandtheforthwelldiscard,add50μltothefifthandthesixthwell,thenaddStandarddilution50μltothefifthandthesixthwell,mix;takeout50μlfromthefifthandthesixthwellandaddtotheseventhandtheeighthwell,thenaddStandarddilution50μltotheseventhandtheeighthwell,mix;takeout50μlfromtheseventhandtheeighthwellandaddtotheninthandthetenthwell,addStandarddilution50μltotheninthandthetenthwell,mix,takeout50μlfromtheninthandthetenthwelldiscard（addSample50μltoeachwellafterDiluting,（density:600pg/ml，400pg/ml，200pg/ml,100pg/ml，50pg/ml）.50pg/ml100pg/ml600pg/ml200pg/ml900pg/ml400pg/ml2.addsample：Setblankwellsseparately（blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame）.testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl（samplefinaldilutionis5-fold）,addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-foldwashsolutiondiluted30-foldwithdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction（thebluecolorchangetoyellowcolor）.11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.CALCULATIONOFRESULTSlCalculatetheaverageabsorbancevaluesforeachsetofstandards,controlsandpatientsamples.lConstructastandardcurvebyplottingthemeanabsorbanceobtainedfromeachstandardagainstits.lconcentrationwithabsorbancevalueonthevertical（Y）axisandconcentrationonthehorizontal（X）axis.lUsingthemeanabsorbancevalueforeachsampledeterminethecorrespondingconcentrationfromthestandardcurve.lAutomatedmethod:TheresultsintheIFUhavebeencalculatedautomaticallyusinga4PL.l（4ParameterLogistics）curvefit.4ParameterLogisticsisthepreferredcalculationmethod.Otherdata.lreductionfunctionsmaygiveslightlydifferentresults.lTheconcentrationofthesamplescanbereaddirectlyfromthisstandardcurve.Sampleswith.lconcentrationshigherthanthatofthehigheststandardhavetobefurtherdiluted.Forthecalculationof.ltheconcentrationsthisdilutionfactorhastobetakenintoaccount.REFERENCESREF:Cat.-No.:/Kat.-Nr.:/No.-Cat.:/Cat.-No.:/N.Cat.:/N.CatLOT:Lot-No.:/Chargen-Bez.:/No.Lot:/Lot-No.:/LoteN.:/Lotton.::No.ofTests:/Kitgre:/Nb.deTests:/No.deDeterm.:/N.deTestes:/Quantitàdeitests::Keepawayfromheatordirectsunlight./VorHitzeunddirekterSonneneinstrahlungschützen./Garderàl’abridelachaleuretdetouteexpositionlumineuse./Manténgasealejadodelcalorolaluzsolardirecta./Manterlongedocalorouluzsolardirecta./Nonesporreairaggisolari.:Readinstructionsbeforeuse./Arbeitsanleitunglesen./Lirelafichetechniqueavantemploi./Lealasinstruccionesantesdeusar./Lerasinstruesantesdeusar./Leggereleistruzioniprimadell’uso.:Storeat:/Lagernbei:/Stockerà:/Almacenea:/Armazenara:/Conservarea:[詳細]

  2018-09-22 10:00

  產(chǎn)品樣冊

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• Mouse （VLDL）ELISA Kit

  Mouseverylowdensitylipoprotein(VLDL)ELISAKitFORRESEARCHUSEONLY.NotforclinicaldiagnosisuseCATALOG#:DAG859INTRODUCTION?ThiskitallowsforthedeterminationofVLDLconcentrationsinMouseserum?Detectionofspecies:Mouse?Detectionmedium:serum,cellculturesupernates.?Assayrange：6.0μg/ml-160μg/mlPRINCIPLEOFTESTThekitassayMouseVLDLlevelinthesample，usePurifiedMouseVLDLantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddVLDLtowells,CombinedVLDLantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofMouseVLDLinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG6342COMPOSITIONOFTHEKIT1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（320μg/ml）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1STORAGECONDITIONS?Theunopenedkitshallbestoredat[2-8℃].?Foropenedkitcanbestoredat[2-8℃]forupto1month.Ifnotbeusedrecently,thestandard首ldbekeptin-20℃.WASHINGMETHOD?Manuallywashingmethod:shakeawaytheremainedliquidintheenzymeplates;placesomebibulouspapersonthetest-bed,andflaptheplatesontheupsidedownstrongly.Injectatleast0.35mlafter-dilutionwashingsolutionintothewell,andmarinate1~2minutes.Repeatthisprocessaccordingtoyourrequirements.?Automaticwashingmethod:ifthereisautomaticwashingmachine,it首ldonlybeusedinthetestwhenyouarequitefamiliarwithitsfunctionandperformance.SAMPLEPREPARATION1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG63432.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.ASSAYPROCEDUREStep1:Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:Step2:Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.Step3:Incubate:Coverwiththeadhesivestripprovided,incubatefor30minat37℃.Step4:Configurateliquid:Dilutewashsolution30-fold(or20-fold)withdistilledwater.Step5:Washing:Uncovertheadhesivestrip,discardliquid,Pipettewashingbuffertoeverywell,stillfor30sthendrain,repeat5times.Step6:Addenzyme:PipetteHRP-Conjugatereagent50μltoeachwell,exceptblankwell.Step7:Incubate:Operationwith3.Step8:Washing:Operationwith5.160μg/ml5Standard150μlOriginaldensityStandard+150μlStandarddiluent80μg/ml4Standard150μl5Standard+150μlStandarddiluent40μg/ml3Standard150μl4Standard+150μlStandarddiluent20μg/ml2Standard150μl3Standard+150μlStandarddiluent10μg/ml1Standard150μl2Standard+150μlStandarddiluentPhone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG6344Step9:Color:PipetteChromogenSolutionA50ulandChromogenSolutionBtoeachwell,avoidthelightpreservationfor15minat37℃Step10:Stopthereaction:PipetteStopSolution50μltoeachwell,Stopthereaction(thebluechangetoyellow).Step11:Calculate:takeblankwellaszero,Readabsorbanceat450nmafterPipetteingStopSolutionwithin15min.CALCULATIONOFRESULTTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.EXPIRATIONSixmonths[seelabelontheouterboxforthespecificdate].Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG634TTENTION?Thekittakesoutfromtherefrigeration首ldbebalanced15-30minutesintheroomtemperature,ifthecoatedELISAplateshavenotbeenusedupafteropening,theplate首ldbestoredinsealedbag.?washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.?addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.?ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.?Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.?Thesubstrate首ldevadethelighttobepreserved.?Pleaserefertotheuserinstructionstrictly,thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.?Thepreparationofsamplesandallthereagents首ldrefertoinfectivematerialprocess.?Donotmixreagentswiththosefromotherlots[詳細]

  2018-10-31 10:00

  產(chǎn)品樣冊

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• 人ELISA試劑盒,TPA ELISA Kit

  96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原（TPA）ELISA試劑盒相關產(chǎn)品：96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原（TPA）ELISA試劑盒IL-10,小鼠白介素-10Elisa試劑盒磺胺二甲基惡唑,標準品JLJL200712人Elisa試劑盒,人ProteinCElisa試劑盒，HumanProteinCELISA試劑盒CAS號:108-69-0,3,5-二,98%人Elisa試劑盒,人CD30Elisa試劑盒，HumanClusterofdifferentiation30,CD30ELISA試劑盒CAS:893-36-7,鹽酸-L-白氨酰-2-萘胺/L-亮氨酰-2-萘胺鹽酸鹽/L-白氨酰-β-萘胺鹽酸鹽/鹽酸-L-亮氨酰-2-萘胺/L（+）-亮氨酰-2-萘基鹽酸氨/L-Leucyl-2-naphthylamidehydrochloride,BR,98%,1克,避光,-20℃96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原（TPA）ELISA試劑盒Humanbacterialvaginosis,BVELISA試劑盒人（BV）kit說明書,細菌性陰道病Elisa試劑盒CXCR3ELISAKit,大鼠CXC趨化因子受體3Elisa檢測試劑盒蒙花苷,標準品,含量測定,20mg,常溫,避光PorcineapoproteinA1,apo-A1ELISA試劑盒豬（apo-A1）kit說明書,載脂蛋白A1Elisa試劑盒大鼠淋巴細胞因子ELISA試劑盒HumanhepatitisBvirusXinteractingprotein,HBXIPELISAKit人異常凝血酶原（APT）ELISA試劑盒HumanAbnormalprothrombin,APTELISA試劑盒草烏甲素,標準品,含量測定,50mg,常溫,避光96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原（TPA）ELISA試劑盒GRP1957,營養(yǎng)肉湯,供一般細菌培養(yǎng)、轉種和增菌用,250g小鼠生長激素釋放多肽（GHRP）ELISA試劑盒HumanMotilin,MTLELISAKitCAS號:4767-3-7,2,2-雙（羥甲基）丙酸,98%[詳細]

  2018-10-23 10:31

  產(chǎn)品樣冊

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• [ELISA]CD8分子（CD8）ELISA Kit

  羊CD8分子（CD8）試劑盒使用說明書本試劑盒僅供研究使用。檢測范圍：96T7IU/ml-240IU/ml試劑盒組成130倍濃縮洗滌液20ml×1瓶7終止液6ml×1瓶2酶標試劑6ml×1瓶8標準品（400IU/ml）0.5ml×1瓶3酶標包被板12孔×8條9標準品稀釋液1.5ml×1瓶4樣品稀釋液6ml×1瓶10說明書1份5顯色劑A液6ml×1瓶11封板膜2張6顯色劑B液6ml×1/瓶12密封袋1個標本要求1．標本采集后盡早進行提取，提取按相關文獻進行，提取后應盡快進行實驗。若不能馬上進行試驗，可將標本放于-20℃保存，但應避免反復凍融2．不能檢測含NaN3的樣品，因NaN3YZ辣根過氧化物酶的（HRP）活性。羊CD8分子ELISA試劑盒用于測定羊血清、血漿及相關液體樣本中CD8分子（CD8）含量。操作步驟1.標準品的稀釋：本試劑盒提供原倍標準品一支，用戶可按照下列圖表在小試管中進行稀釋。200IU/ml5號標準品150μl的原倍標準品加入150μl標準品稀釋液100IU/ml4號標準品150μl的5號標準品加入150μl標準品稀釋液50IU/ml3號標準品150μl的4號標準品加入150μl標準品稀釋液25IU/ml2號標準品150μl的3號標準品加入150μl標準品稀釋液12.5IU/ml1號標準品150μl的2號標準品加入150μl標準品稀釋液2.加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑，其余各步操作相同）、標準孔、待測樣品孔。在酶標包被板上標準品準確加樣50μl，待測樣品孔中先加樣品稀釋液40μl，然后再加待測樣品10μl（樣品Z終稀釋度為5倍）。加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻。3.溫育：用封板膜封板后置37℃溫育30分鐘。4.配液：將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用5.洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復5次，拍干。6.加酶：每孔加入酶標試劑50μl，空白孔除外。7.溫育：操作同3。8.洗滌：操作同5。9.顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘.10.終止：每孔加終止液50μl，終止反應（此時藍色立轉黃色）。11.測定：以空白空調(diào)零，450nm波長依序測量各孔的吸光度（OD值）。測定應在加終止液后15分鐘以內(nèi)進行。羊CD8分子ELISA試劑盒注意事項1．試劑盒從冷藏環(huán)境中取出應在室溫平衡15-30分鐘后方可使用，酶標包被板開封后如未用完，板條應裝入密封袋中保存。2．濃洗滌液可能會有結晶析出，稀釋時可在水浴中加溫助溶，洗滌時不影響結果。3．各步加樣均應使用加樣器，并經(jīng)常校對其準確性，以避免試驗誤差。一次加樣時間**控制在5分鐘內(nèi)，如標本數(shù)量多，推薦使用排槍加樣。4．請每次測定的同時做標準曲線，**做復孔。如標本中待測物質含量過高（樣本OD值大于標準品孔**孔的OD值），請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定，計算時請Z后乘以總稀釋倍數(shù)（×n×5）。5．封板膜只限一次性使用，以避免交叉污染。6．底物請避光保存。7．嚴格按照說明書的操作進行，試驗結果判定必須以酶標儀讀數(shù)為準.8．所有樣品，洗滌液和各種廢棄物都應按傳染物處理。9．本試劑不同批號組分不得混用。10.如與英文說明書有異，以英文說明書為準。保存條件及有效期1．試劑盒保存：；2-8℃。2．有效期：6個月羊CD8分子ELISA試劑盒應用雙抗體夾心法測定標本中羊CD8分子（CD8）水平。用純化的羊CD8分子（CD8）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入CD8分子（CD8），再與HRP標記的CD8分子（CD8）抗體結合，形成抗體-抗原-酶標抗體復合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，并在酸的作用下轉化成Z終的黃色。顏色的深淺和樣品中的CD8分子（CD8）呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中羊CD8分子（CD8）濃度。[詳細]

  2018-11-16 10:02

  產(chǎn)品樣冊

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• 豬血纖蛋白原降解產(chǎn)物(FDP)ELISA試劑盒

  豬血纖蛋白原降解產(chǎn)物(FDP)ELISA試劑盒[詳細]

  2013-12-12 00:00

  課件

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• 鯉魚卵黃蛋白原（VTG）Elisa試劑盒使用說明書

  本試劑盒用于測定鯉魚血清、血漿及相關液體樣本中卵黃蛋白原（VTG）含量。實驗原理本試劑盒應用雙抗體夾心法測定標本中鯉魚卵黃蛋白原（VTG）水平。用純化的鯉魚卵黃蛋白原（VTG）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入卵黃蛋白（VTG），再與HRP標記的卵黃蛋白原（VTG）抗體結合，形成抗體-抗原-酶標抗體復合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉化成藍色，并在酸的作用下轉化成Z終的黃色。顏色的深淺和樣品中的卵黃蛋白原（VTG）呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中鯉魚卵黃蛋白原（VTG）濃度。試劑盒組成130倍濃縮洗滌液20ml×1瓶7終止液6ml×1瓶2酶標試劑6ml×1瓶8標準品（1600μg/L）0.5ml×1瓶3酶標包被板12孔×8條9標準品稀釋液1.5ml×1瓶4樣品稀釋液6ml×1瓶10說明書1份5顯色劑A液6ml×1瓶11封板膜2張6顯色劑B液6ml×1/瓶12密封袋1個標本要求1．標本采集后盡早進行提取，提取按相關文獻進行，提取后應盡快進行實驗。若不能馬上進行試驗，可將標本放于-20℃保存，但應避免反復凍融2．不能檢測含NaN3的樣品，因NaN3YZ辣根過氧化物酶的（HRP）活性。操作步驟1.標準品的稀釋：本試劑盒提供原倍標準品一支，用戶可按照下列圖表在小試管中進行稀釋。800μg/L5號標準品150μl的原倍標準品加入150μl標準品稀釋液400μg/L4號標準品150μl的5號標準品加入150μl標準品稀釋液200μg/L3號標準品150μl的4號標準品加入150μl標準品稀釋液100μg/L2號標準品150μl的3號標準品加入150μl標準品稀釋液50μg/L1號標準品150μl的2號標準品加入150μl標準品稀釋液2.加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑，其余各步操作相同）、標準孔、待測樣品孔。在酶標包被板上標準品準確加樣50μl，待測樣品孔中先加樣品稀釋液40μl，然后再加待測樣品10μl（樣品Z終稀釋度為5倍）。加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻。3.溫育：用封板膜封板后置37℃溫育30分鐘。4.配液：將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用5.洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復5次，拍干。6.加酶：每孔加入酶標試劑50μl，空白孔除外。7.溫育：操作同3。8.洗滌：操作同5。9.顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘.10.終止：每孔加終止液50μl，終止反應（此時藍色立轉黃色）。11.測定：以空白空調(diào)零，450nm波長依序測量各孔的吸光度（OD值）。測定應在加終止液后15分鐘以內(nèi)進行。計算以標準物的濃度為橫坐標，OD值為縱坐標，在坐標紙上繪出標準曲線，根據(jù)樣品的OD值由標準曲線查出相應的濃度；再乘以稀釋倍數(shù)；或用標準物的濃度與OD值計算出標準曲線的直線回歸方程式，將樣品的OD值代入方程式，計算出樣品濃度，再乘以稀釋倍數(shù)，即為樣品的實際濃度。[詳細]

  2018-09-19 10:00

  產(chǎn)品樣冊

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