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Human AMH ELISA kit
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2015-10-09 00:00 556閱讀次數(shù)
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Human AMH ELISA kit
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www.biokanu.comRatamyloidbetapeptide1-42(Aβ1-42)ELISAKitProd.No.3R285FROM:RBForthequantitativeinvitrodeterminationofAβ1-42concentrationsinRatsupernates,serum,plasmaandtissue.FORRESEARCHUSEONLY.NOTFORUSEINDIAGNOSTICPROCEDURES.TABLEOFCONTENTSContentsPageTABLEOFCONTENTS..2INTENDEDUSE..3PRINCIPLE..3WARNINGSANDPRECAUTIONS..4MATERIALSPROVIDEDWITHTHEKIT.7MATERIALSREQUIREDBUTNOTPROVIDED..7STORAGECONDITIONS..8REAGENTPREPARATION..9SPECIMENCOLLECTIONANDPREPARATION..9ASSAYPROCEDURE..10CALCULATIONOFRESULTS..13REFERENCES..14INTENDEDUSEAnenzymeimmunoassayforthequantitativeinvitrodiagnosticmeasurementofRatAβ1-42incellculturesupernates,serum,plasmaandtissue.PRINCIPLEThekitassayRatAβ1-42levelinthesample,usePurifiedRatAβ1-42antibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddAβ1-42towells,CombinedAβ1-42antibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofAβ1-42inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.WARNINGSANDPRECAUTIONSlThiskitisforinvitrodiagnosticuseonly.Forprofessionaluseonly.lAllreagentsofthistestkitwhichcontainhumanserumorplasmahavebeentestedandconfirmednegativeforHIVI/II,HBsAgandHCVbyFDAapprovedprocedures.Allreagents,however,首ldbetreatedaspotentialbiohazardsinuseandfordisposal.lBeforestartingtheassay,readtheinstructionscompletelyandcarefully.Usethevalidversionofthepackageinsertprovidedwiththekit.Besurethateverythingisunderstood.lThemicroplatecontainssnap-offstrips.Unusedwellsmustbestoredat2°Cto8°Cinthesealedfoilpouchandusedintheframeprovided.lPipettingofsamplesandreagentsmustbedoneasquicklyaspossibleandinthesamesequenceforeachstep.lUsereservoirsonlyforsinglereagents.Thisespeciallyappliestothesubstratereservoirs.Usingareservoirfordispensingasubstratesolutionthathadpreviouslybeenusedfortheconjugatesolutionmayturnsolutioncolored.Donotpourreagentsbackintovialsasreagentcontaminationmayoccur.lMixthecontentsofthemicroplatewellsthoroughlytoensuregoodtestresults.Donotreusemicrowells.lDonotletwellsdryduringassay;addreagentsimmediatelyaftercompletingtherinsingsteps.lAllowthereagentstoreachroomtemperature(21-26°C)beforestartingthetest.Temperaturewillaffecttheabsorbancereadingsoftheassay.However,valuesforthepatientsampleswillnotbeaffected.lNeverpipetbymouthandavoidcontactofreagentsandspecimenswithskinandmucousmembranes.lDonotsmoke,eat,drinkorapplycosmeticsinareaswherespecimensorkitreagentsarehandled.lWeardisposablelatexgloveswhenhand領(lǐng)specimensandreagents.Microbialcontaminationofreagentsorspecimensmaygivefalseresults.lHand領(lǐng)首ldbedoneinaccordancewiththeproceduresdefinedbyanappropriatenationalbiohazardsafetyguidelineorregulation.lDonotusereagentsbeyondexpirydateasshownonthekitlabels.lAllindicatedvolumeshavetobeperformedaccordingtotheprotocol.Optimaltestresultsareonlyobtainedwhenusingcalibratedpipettesandmicrotiterplatereaders.lDonotmixorusecomponentsfromkitswithdifferentlotnumbers.Itisadvisednottoexchangewellsofdifferentplatesevenofthesamelot.Thekitsmayhavebeenshippedorstoredunderdifferentconditionsandthebindingcharacteristicsoftheplatesmayresultslightlydifferent.lAvoidcontactwithStopSolutioncontaining0.5MH2SO4.Itmaycauseskinirritationandburns.lSomereagentscontainProclin,BNDand/orMITaspreservatives.Incaseofcontactwitheyesorskin,flushimmediatelywithwater.lTMBsubstratehasanirritanteffectonskinandmucosa.Incaseofpossiblecontact,washeyeswithanabundantvolumeofwaterandskinwithsoapandabundantwater.Washcontaminatedobjectsbeforereusingthem.Ifinhaled,takethepersontoopenair.lChemicalsandpreparedorusedreagentshavetobetreatedashazardouswasteaccordingtothenationalbiohazardsafetyguidelineorregulation.lForinformationonhazardoussubstancesincludedinthekitpleaserefertoMaterialSafetyDataSheetsMATERIALSPROVIDEDWITHTHEKITMaterialsprovidedwiththekit96determinationsStorageUsermanual1Closureplatemembrane2Sealedbags1Microelisastripplate12-8℃Standard:900pg/ml0.5ml×1bottle2-8℃Standarddiluent1.5ml×1bottle2-8℃HRP-Conjugatereagent6ml×1bottle2-8℃Samplediluent6ml×1bottle2-8℃ChromogenSolutionA6ml×1bottle2-8℃ChromogenSolutionB6ml×1bottle2-8℃StopSolution6ml×1bottle2-8℃washsolution30×20ml×1bottle2-8℃MATERIALSREQUIREDBUTNOTPROVIDEDlMicroplatereadercapableofmeasuringabsorbanceat450nm.lPrecisionpipettestodeliver2mlto1mlvolumes.l100mland1litergraduatedcylinders.lCalibratedadjustableprecisionpipettes,preferablywithdisposableplastictips.(Amanifoldmulti-channelpipetteisdesirableforlargeassays.)lAbsorbentpaper.l37°Cincubator.lDistilledordeionizedwater.lDataanalysisandgraphingsoftware.Graphpaper:linear(Cartesian),log-logorsemi-log,orlog-logitasdesired.lTubestopreparestandardorsampledilutions.STORAGECONDITIONSuWhenstoredat2°Cto8°Cunopenedreagentswillretainreactivityuntilexpirationdate.uDonotusereagentsbeyondthisdate.Openedreagentsmustbestoredat2°Cto8°C.uMicrotiterwellsmustbestoredat2°Cto8°C.Oncethefoilbaghasbeenopened,care首ldbetakentocloseittightlyagain.uOpenedkitsretainactivityfor8weeksifstoredasdescribedabove.REAGENTPREPARATIONBringallreagentstoroomtemperaturebeforeuseSPECIMENCOLLECTIONANDPREPARATIONSerum-Useaserumseparatortube(SST)andallowsamplestoclotfor30minutesbeforecentrifugationfor15minutesatapproximately1000xg.Removeserumandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Plasma-CollectplasmausingEDTAorheparinasananticoagulant.Centrifugesamplesfor15minutesat1000xgat2-8°Cwithin30minutesofcollection.Storesamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcycles.Cellculturefluidandotherbiologicalfluids-Removeparticulatesbycentrifugationandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcyclesASSAYPROCEDUREuGeneralRemarkslAllreagentsandspecimensmustbeallowedtocometoroomtemperaturebeforeuse.Allreagentsmustbemixedwithoutfoaming.lOncethetesthasbeenstarted,allsteps首ldbecompletedwithoutinterruption.lUsenewdisposalplasticpipettetipsforeachstandard,controlorsampleinordertoavoidcrosscontamination.lAbsorbanceisafunctionoftheincubationtimeandtemperature.Beforestartingtheassay,itisrecommendedthatallreagentsareready,capsremoved,allneededwellssecuredinholder,etc.Thiswillensureequalelapsedtimeforeachpipettingstepwithoutinterruption.lAsageneralruletheenzymaticreactionislinearlyproportionaltotimeandtemperature.lDetermineabsorptionwithanELISAreaderat450nmagainst620nmasreference.Ifnoreferencewavelengthisavailable,readonlyat450nm.Iftheextinctionofthehigheststandardexceedsthemeasurementrangeofthephotometer,absorptionmustbemeasuredimmediatelyat405nmagainst620nmasreference.uAssayProcedure1.DiluteandaddsampletoStandard:set10StandardwellsontheELISAplatescoated,addStandard100μltothefirstandthesecondwell,thenaddStandarddilution50μltothefirstandthesecondwell,mix;takeout100μlformthefirstandthesecondwellthenaddittothethirdandtheforthwellseparately.thenaddStandarddilution50μltothethirdandtheforthwell,mix;thentakeout50μlfromthethirdandtheforthwelldiscard,add50μltothefifthandthesixthwell,thenaddStandarddilution50μltothefifthandthesixthwell,mix;takeout50μlfromthefifthandthesixthwellandaddtotheseventhandtheeighthwell,thenaddStandarddilution50μltotheseventhandtheeighthwell,mix;takeout50μlfromtheseventhandtheeighthwellandaddtotheninthandthetenthwell,addStandarddilution50μltotheninthandthetenthwell,mix,takeout50μlfromtheninthandthetenthwelldiscard(addSample50μltoeachwellafterDiluting,(density:600pg/ml,400pg/ml,200pg/ml,100pg/ml,50pg/ml).50pg/ml100pg/ml600pg/ml200pg/ml900pg/ml400pg/ml2.addsample:Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-foldwashsolutiondiluted30-foldwithdistilledwaterandreserve.5.washing:UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme:AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate:Operationwith3.8.washing:Operationwith5.9.color:AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction:AddStopSolution50μltoeachwell,Stopthereaction(thebluecolorchangetoyellowcolor).11.assay:takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.CALCULATIONOFRESULTSlCalculatetheaverageabsorbancevaluesforeachsetofstandards,controlsandpatientsamples.lConstructastandardcurvebyplottingthemeanabsorbanceobtainedfromeachstandardagainstits.lconcentrationwithabsorbancevalueonthevertical(Y)axisandconcentrationonthehorizontal(X)axis.lUsingthemeanabsorbancevalueforeachsampledeterminethecorrespondingconcentrationfromthestandardcurve.lAutomatedmethod:TheresultsintheIFUhavebeencalculatedautomaticallyusinga4PL.l(4ParameterLogistics)curvefit.4ParameterLogisticsisthepreferredcalculationmethod.Otherdata.lreductionfunctionsmaygiveslightlydifferentresults.lTheconcentrationofthesamplescanbereaddirectlyfromthisstandardcurve.Sampleswith.lconcentrationshigherthanthatofthehigheststandardhavetobefurtherdiluted.Forthecalculationof.ltheconcentrationsthisdilutionfactorhastobetakenintoaccount.REFERENCESREF:Cat.-No.:/Kat.-Nr.:/No.-Cat.:/Cat.-No.:/N.Cat.:/N.CatLOT:Lot-No.:/Chargen-Bez.:/No.Lot:/Lot-No.:/LoteN.:/Lotton.::No.ofTests:/Kitgre:/Nb.deTests:/No.deDeterm.:/N.deTestes:/Quantitàdeitests::Keepawayfromheatordirectsunlight./VorHitzeunddirekterSonneneinstrahlungschützen./Garderàl’abridelachaleuretdetouteexpositionlumineuse./Manténgasealejadodelcalorolaluzsolardirecta./Manterlongedocalorouluzsolardirecta./Nonesporreairaggisolari.:Readinstructionsbeforeuse./Arbeitsanleitunglesen./Lirelafichetechniqueavantemploi./Lealasinstruccionesantesdeusar./Lerasinstruesantesdeusar./Leggereleistruzioniprimadell’uso.:Storeat:/Lagernbei:/Stockerà:/Almacenea:/Armazenara:/Conservarea:[詳細(xì)]
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Mouse (VLDL)ELISA Kit
- Mouseverylowdensitylipoprotein(VLDL)ELISAKitFORRESEARCHUSEONLY.NotforclinicaldiagnosisuseCATALOG#:DAG859INTRODUCTION?ThiskitallowsforthedeterminationofVLDLconcentrationsinMouseserum?Detectionofspecies:Mouse?Detectionmedium:serum,cellculturesupernates.?Assayrange:6.0μg/ml-160μg/mlPRINCIPLEOFTESTThekitassayMouseVLDLlevelinthesample,usePurifiedMouseVLDLantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddVLDLtowells,CombinedVLDLantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofMouseVLDLinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG6342COMPOSITIONOFTHEKIT1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard(320μg/ml)0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1STORAGECONDITIONS?Theunopenedkitshallbestoredat[2-8℃].?Foropenedkitcanbestoredat[2-8℃]forupto1month.Ifnotbeusedrecently,thestandard首ldbekeptin-20℃.WASHINGMETHOD?Manuallywashingmethod:shakeawaytheremainedliquidintheenzymeplates;placesomebibulouspapersonthetest-bed,andflaptheplatesontheupsidedownstrongly.Injectatleast0.35mlafter-dilutionwashingsolutionintothewell,andmarinate1~2minutes.Repeatthisprocessaccordingtoyourrequirements.?Automaticwashingmethod:ifthereisautomaticwashingmachine,it首ldonlybeusedinthetestwhenyouarequitefamiliarwithitsfunctionandperformance.SAMPLEPREPARATION1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG63432.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.ASSAYPROCEDUREStep1:Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:Step2:Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.Step3:Incubate:Coverwiththeadhesivestripprovided,incubatefor30minat37℃.Step4:Configurateliquid:Dilutewashsolution30-fold(or20-fold)withdistilledwater.Step5:Washing:Uncovertheadhesivestrip,discardliquid,Pipettewashingbuffertoeverywell,stillfor30sthendrain,repeat5times.Step6:Addenzyme:PipetteHRP-Conjugatereagent50μltoeachwell,exceptblankwell.Step7:Incubate:Operationwith3.Step8:Washing:Operationwith5.160μg/ml5Standard150μlOriginaldensityStandard+150μlStandarddiluent80μg/ml4Standard150μl5Standard+150μlStandarddiluent40μg/ml3Standard150μl4Standard+150μlStandarddiluent20μg/ml2Standard150μl3Standard+150μlStandarddiluent10μg/ml1Standard150μl2Standard+150μlStandarddiluentPhone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG6344Step9:Color:PipetteChromogenSolutionA50ulandChromogenSolutionBtoeachwell,avoidthelightpreservationfor15minat37℃Step10:Stopthereaction:PipetteStopSolution50μltoeachwell,Stopthereaction(thebluechangetoyellow).Step11:Calculate:takeblankwellaszero,Readabsorbanceat450nmafterPipetteingStopSolutionwithin15min.CALCULATIONOFRESULTTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.EXPIRATIONSixmonths[seelabelontheouterboxforthespecificdate].Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG634TTENTION?Thekittakesoutfromtherefrigeration首ldbebalanced15-30minutesintheroomtemperature,ifthecoatedELISAplateshavenotbeenusedupafteropening,theplate首ldbestoredinsealedbag.?washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.?addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.?ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.(×n×5).?Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.?Thesubstrate首ldevadethelighttobepreserved.?Pleaserefertotheuserinstructionstrictly,thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.?Thepreparationofsamplesandallthereagents首ldrefertoinfectivematerialprocess.?Donotmixreagentswiththosefromotherlots[詳細(xì)]
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2018-10-31 10:00
產(chǎn)品樣冊
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Human 8-OHDG elisa試劑盒
- Human 8-OHDG elisa試劑盒[詳細(xì)]
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2024-09-16 18:47
期刊論文
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人ELISA試劑盒,TPA ELISA Kit
- 96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原(TPA)ELISA試劑盒相關(guān)產(chǎn)品:96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原(TPA)ELISA試劑盒IL-10,小鼠白介素-10Elisa試劑盒磺胺二甲基惡唑,標(biāo)準(zhǔn)品JLJL200712人Elisa試劑盒,人ProteinCElisa試劑盒,HumanProteinCELISA試劑盒CAS號:108-69-0,3,5-二,98%人Elisa試劑盒,人CD30Elisa試劑盒,HumanClusterofdifferentiation30,CD30ELISA試劑盒CAS:893-36-7,鹽酸-L-白氨酰-2-萘胺/L-亮氨酰-2-萘胺鹽酸鹽/L-白氨酰-β-萘胺鹽酸鹽/鹽酸-L-亮氨酰-2-萘胺/L(+)-亮氨酰-2-萘基鹽酸氨/L-Leucyl-2-naphthylamidehydrochloride,BR,98%,1克,避光,-20℃96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原(TPA)ELISA試劑盒Humanbacterialvaginosis,BVELISA試劑盒人(BV)kit說明書,細(xì)菌性陰道病Elisa試劑盒CXCR3ELISAKit,大鼠CXC趨化因子受體3Elisa檢測試劑盒蒙花苷,標(biāo)準(zhǔn)品,含量測定,20mg,常溫,避光PorcineapoproteinA1,apo-A1ELISA試劑盒豬(apo-A1)kit說明書,載脂蛋白A1Elisa試劑盒大鼠淋巴細(xì)胞因子ELISA試劑盒HumanhepatitisBvirusXinteractingprotein,HBXIPELISAKit人異常凝血酶原(APT)ELISA試劑盒HumanAbnormalprothrombin,APTELISA試劑盒草烏甲素,標(biāo)準(zhǔn)品,含量測定,50mg,常溫,避光96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原(TPA)ELISA試劑盒GRP1957,營養(yǎng)肉湯,供一般細(xì)菌培養(yǎng)、轉(zhuǎn)種和增菌用,250g小鼠生長激素釋放多肽(GHRP)ELISA試劑盒HumanMotilin,MTLELISAKitCAS號:4767-3-7,2,2-雙(羥甲基)丙酸,98%[詳細(xì)]
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2018-10-23 10:31
產(chǎn)品樣冊
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[ELISA]CD8分子(CD8)ELISA Kit
- 羊CD8分子(CD8)試劑盒使用說明書本試劑盒僅供研究使用。檢測范圍:96T7IU/ml-240IU/ml試劑盒組成130倍濃縮洗滌液20ml×1瓶7終止液6ml×1瓶2酶標(biāo)試劑6ml×1瓶8標(biāo)準(zhǔn)品(400IU/ml)0.5ml×1瓶3酶標(biāo)包被板12孔×8條9標(biāo)準(zhǔn)品稀釋液1.5ml×1瓶4樣品稀釋液6ml×1瓶10說明書1份5顯色劑A液6ml×1瓶11封板膜2張6顯色劑B液6ml×1/瓶12密封袋1個標(biāo)本要求1.標(biāo)本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融2.不能檢測含NaN3的樣品,因NaN3YZ辣根過氧化物酶的(HRP)活性。羊CD8分子ELISA試劑盒用于測定羊血清、血漿及相關(guān)液體樣本中CD8分子(CD8)含量。操作步驟1.標(biāo)準(zhǔn)品的稀釋:本試劑盒提供原倍標(biāo)準(zhǔn)品一支,用戶可按照下列圖表在小試管中進行稀釋。200IU/ml5號標(biāo)準(zhǔn)品150μl的原倍標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液100IU/ml4號標(biāo)準(zhǔn)品150μl的5號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液50IU/ml3號標(biāo)準(zhǔn)品150μl的4號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液25IU/ml2號標(biāo)準(zhǔn)品150μl的3號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液12.5IU/ml1號標(biāo)準(zhǔn)品150μl的2號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液2.加樣:分別設(shè)空白孔(空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、標(biāo)準(zhǔn)孔、待測樣品孔。在酶標(biāo)包被板上標(biāo)準(zhǔn)品準(zhǔn)確加樣50μl,待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl(樣品Z終稀釋度為5倍)。加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻。3.溫育:用封板膜封板后置37℃溫育30分鐘。4.配液:將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用5.洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。6.加酶:每孔加入酶標(biāo)試劑50μl,空白孔除外。7.溫育:操作同3。8.洗滌:操作同5。9.顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.10.終止:每孔加終止液50μl,終止反應(yīng)(此時藍色立轉(zhuǎn)黃色)。11.測定:以空白空調(diào)零,450nm波長依序測量各孔的吸光度(OD值)。測定應(yīng)在加終止液后15分鐘以內(nèi)進行。羊CD8分子ELISA試劑盒注意事項1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。2.濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗誤差。一次加樣時間**控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。4.請每次測定的同時做標(biāo)準(zhǔn)曲線,**做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔**孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請Z后乘以總稀釋倍數(shù)(×n×5)。5.封板膜只限一次性使用,以避免交叉污染。6.底物請避光保存。7.嚴(yán)格按照說明書的操作進行,試驗結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。9.本試劑不同批號組分不得混用。10.如與英文說明書有異,以英文說明書為準(zhǔn)。保存條件及有效期1.試劑盒保存:;2-8℃。2.有效期:6個月羊CD8分子ELISA試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中羊CD8分子(CD8)水平。用純化的羊CD8分子(CD8)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入CD8分子(CD8),再與HRP標(biāo)記的CD8分子(CD8)抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成Z終的黃色。顏色的深淺和樣品中的CD8分子(CD8)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計算樣品中羊CD8分子(CD8)濃度。[詳細(xì)]
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2018-11-16 10:02
產(chǎn)品樣冊
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人黑色素ELISA Kit說明書
- 人黑色素ELISA Kit說明書[詳細(xì)]
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2014-08-21 00:00
安裝說明
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Kainos FGF-23 ELISA Kit說明書
- KainosLaboratories是日本的生物公司,其生產(chǎn)的FGF-23ELISAKit和ADAMTS13-cleaved廣泛應(yīng)用于各大醫(yī)藥企業(yè),上海起發(fā)實驗試劑有限公司正式為您帶來KainosLaboratories高品質(zhì)的產(chǎn)品,歡迎來電查詢http://www.kainos.co.jpKainosLaboratories代理KainosLaboratoriesZG代理,KainosLaboratories上海代理,KainosLaboratories總代,KainosLaboratories北京代理ThiskitcapturesADAMTS13-cleavedproductsusingasandwichmethodwiththeanti-GSTmousemonoclonalantibodyandtheperoxidase-conjugatedmousemonoclonalanti-N10antibody.First,theanti-GSTmousemonoclonalantibodyimmobilizedontothemicroplatereactswiththeVWF73substrate(GST-VWF73-His).Thesampleisthenappliedtothemicroplate,onwhichADAMTS13cleavestheVWF73substrate.Byapplyingthemousemonoclonalanti-N10antibodyconjugatedwithhorseradishperoxidase(HRPconjugatedantibody),thecleavageproductissandwichedbetweenthetwoantibodies.BecausetheamountofthecleavageproductsdependsontheADAMTS13activity,theamountoftheenzyme-labeledantibodiesthatbindtothecleavageproductsalsoreflectthelevelofADAMTS13activity.Therefore,theplasmaADAMTS13activitycanbedeterminedbycolorimetricallymeasuringtheamountofdetachedoxidized(colored)TMBZusingureahydrogenperoxide(H2O2)and3,3’,5,5’-Tetramethyl-benzidine(TMBZ)asthesubstrates.上海起發(fā)實驗試劑有限公司實驗試劑一站式采購服務(wù)商1:強大的進口輻射能力,血清、抗體、耗材、大部分限制進口品等。2:產(chǎn)品種類齊全,經(jīng)營超過700多個品牌,基本涵蓋所有生物實驗試劑耗材。3:提供加急服務(wù),貨品一般1-2周到貨。4:富有競爭力的價格優(yōu)勢,絕大部分價格有優(yōu)勢。5:多年積累良好的信譽,大部分客戶提供貨到付款服務(wù)??蛻舭ㄇ迦A、北大、交大、復(fù)旦、中山等100多所高校,ROCHE,阿斯利康、國藥、fisher等知名藥企。6:我們還是Santa,AdvancedBiotechnologiesInc,AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc,Genebridege,GlycotopeBiotechnologyGmbH;iduron,InnovativeResearchofAmerica,Ludger,neuroprobe,omicronbio,Polysciences,prospecbi,QA-BIO,quickzyme,RESEARCHDIETS,INC,sterlitech;sysy,TriLinkBioTechnologies,Inc;worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。7:我們還是invitrogen,qiagen,Tempshield,Inc.am,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā),歡迎合作。[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊
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