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2015-10-09 00:00 451閱讀次數(shù)

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• Human PLA2R1 ELISA kit

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• Human AMH ELISA kit

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• Human interleukin-17(IL-17) ELISA kit

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• Human uPAR Quantikine ELISA Kit

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• Human HMW Adiponectin Quantikine ELISA Kit

  HumanHMWAdiponectinQuantikineELISAKit[詳細(xì)]

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• 人（Human）白介素1α（IL-1α）ELISA Kit使用說明書

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• 人白介素-22結(jié)合蛋白（IL-22BP）ELISA試劑盒說明書

  人白介素-22結(jié)合蛋白（IL-22BP）ELISA試劑盒（用于血清、血漿、細(xì)胞培養(yǎng)上清液和其它生物體液內(nèi)）原理本實(shí)驗(yàn)采用雙抗體夾心ABC-ELISA法。用抗人IL-22BP單抗包被于酶標(biāo)板上，標(biāo)準(zhǔn)品和樣品中的IL-22BP與單抗結(jié)合，加入生物素化的抗人IL-22BP，形成免疫復(fù)合物連接在板上，辣根過氧化物酶標(biāo)記的Streptavidin與生物素結(jié)合，加入底物工作液顯藍(lán)色，Z后加終止液，在450nm處測(cè)OD值，IL-22BP濃度與OD值成正比，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中IL-22BP濃度。試劑盒組成（2-8℃保存）酶標(biāo)板（CoatedWells）96孔酶標(biāo)抗體工作液（EnzymeConjugate）12ml10×標(biāo)本稀釋液（SampleBuffer）12ml20×濃縮洗滌液（WashBuffer）50ml標(biāo)準(zhǔn)品（Standards）：20ng/瓶2瓶底物工作液（TMBSolution）12ml**抗體工作液（BiotinylatedAntibody）12ml終止液（StopSolution）12ml準(zhǔn)備試劑與收集血樣1.收集標(biāo)本：血清、血漿（EDTA、檸檬酸鹽、肝素抗凝）、細(xì)胞培養(yǎng)上清液、組織勻漿等盡早檢測(cè)，2-8℃保存48小時(shí)；更長(zhǎng)時(shí)間須冷凍（-20℃或-70℃）保存，避免反復(fù)凍融。血清、血漿、細(xì)胞培養(yǎng)上清至少作1：5稀釋（取50ul，加標(biāo)本稀釋液200ul,稀釋5倍）。2.標(biāo)準(zhǔn)品液配制：使用前加入1ml蒸餾水混勻，配成20ng/ml的溶液。設(shè)標(biāo)準(zhǔn)管8管，**管加標(biāo)本稀釋液900ul，第二至第八管加入標(biāo)本稀釋液500ul。在**管中加入20ng/ml的標(biāo)準(zhǔn)品溶液100ul混勻后用加樣器吸出500ul，移至第二管。如此反復(fù)作對(duì)倍稀釋，從第七管中吸出500ul棄去。第八管為空白對(duì)照。3.10×標(biāo)本稀釋液用蒸餾水作1:10倍稀釋（示例：1ml濃稀釋液+9ml蒸餾水）。4.洗滌液：用重蒸水1:20稀釋（示例：1ml濃縮洗滌液加入19ml的重蒸水）檢測(cè)程序1.加樣：每孔各加入標(biāo)準(zhǔn)品或待測(cè)樣品100ul，將反應(yīng)板充分混勻后置37℃120分鐘。2.洗板：用洗滌液將反應(yīng)板充分洗滌4-6次，向?yàn)V紙上印干。3.每孔中加入**抗體工作液100ul。將反應(yīng)板充分混勻后置37℃60分鐘。4.洗板：同前。5.每孔加酶標(biāo)抗體工作液100ul。將反應(yīng)板置37℃30分鐘。6.洗板：同前。7.每孔加入底物工作液100ul，置37℃暗處反應(yīng)15分鐘。8.每孔加入100ul終止液混勻。9.30分鐘內(nèi)用酶標(biāo)儀在450nm處測(cè)吸光值。結(jié)果計(jì)算與判斷1.所有OD值都應(yīng)減除空白值后再行計(jì)算。2.以標(biāo)準(zhǔn)品2000、1000、500、250、125、62.5、31.2、0pg/ml為橫坐標(biāo)，OD值為縱坐標(biāo)，在坐標(biāo)紙上作圖，畫出標(biāo)準(zhǔn)曲線。3.根據(jù)樣品OD值在該曲線圖上查出相應(yīng)IL-22BP含量，再乘上稀釋倍數(shù)即可。試劑盒性能1.靈敏度：Z小的IL-22BP檢測(cè)濃度小于15pg/ml。2.特異性：可同時(shí)檢測(cè)重組或天然的人IL-22BP。不與人其它細(xì)胞因子有交叉反應(yīng)。3.重復(fù)性：板內(nèi)、板見變異系數(shù)均小于10.2％。注意事項(xiàng)1.以上標(biāo)準(zhǔn)孔及待測(cè)樣品均建議做復(fù)孔，每次測(cè)定應(yīng)同時(shí)做標(biāo)準(zhǔn)曲線。2.洗滌過程很關(guān)鍵。洗滌不充分將導(dǎo)致極ng確度誤差及OD值錯(cuò)誤地升高。3.板條開封后剩余板條要再封好，保持板條干燥。4.本試劑盒宜置4oC冰箱保存。5.本試劑盒僅用于科研，不能用于臨床診斷！[詳細(xì)]

  2018-09-13 10:01

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• Monkey ELISA Kit

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• 上海易利為您提供Human GST elisa kit 英文說明書

  本公司為ELISA試劑盒供應(yīng)商，價(jià)格公正，售前，售中，售后全程為您服務(wù)。提供免費(fèi)代測(cè)服務(wù)，一周內(nèi)出結(jié)果，保證質(zhì)量歡迎來電咨詢!許經(jīng)理021-6051540918217407991[詳細(xì)]

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• 人凝血酶ELISA Kit

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  2014-08-21 00:00

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• Mouse CaSR ELISA kit

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  2015-10-09 00:00

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• Rat Aβ1-42 ELISA kit

  www.biokanu.comRatamyloidbetapeptide1-42（Aβ1-42）ELISAKitProd.No.3R285FROM:RBForthequantitativeinvitrodeterminationofAβ1-42concentrationsinRatsupernates,serum,plasmaandtissue.FORRESEARCHUSEONLY.NOTFORUSEINDIAGNOSTICPROCEDURES.TABLEOFCONTENTSContentsPageTABLEOFCONTENTS..2INTENDEDUSE..3PRINCIPLE..3WARNINGSANDPRECAUTIONS..4MATERIALSPROVIDEDWITHTHEKIT.7MATERIALSREQUIREDBUTNOTPROVIDED..7STORAGECONDITIONS..8REAGENTPREPARATION..9SPECIMENCOLLECTIONANDPREPARATION..9ASSAYPROCEDURE..10CALCULATIONOFRESULTS..13REFERENCES..14INTENDEDUSEAnenzymeimmunoassayforthequantitativeinvitrodiagnosticmeasurementofRatAβ1-42incellculturesupernates,serum,plasmaandtissue.PRINCIPLEThekitassayRatAβ1-42levelinthesample，usePurifiedRatAβ1-42antibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddAβ1-42towells,CombinedAβ1-42antibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofAβ1-42inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.WARNINGSANDPRECAUTIONSlThiskitisforinvitrodiagnosticuseonly.Forprofessionaluseonly.lAllreagentsofthistestkitwhichcontainhumanserumorplasmahavebeentestedandconfirmednegativeforHIVI/II,HBsAgandHCVbyFDAapprovedprocedures.Allreagents,however,首ldbetreatedaspotentialbiohazardsinuseandfordisposal.lBeforestartingtheassay,readtheinstructionscompletelyandcarefully.Usethevalidversionofthepackageinsertprovidedwiththekit.Besurethateverythingisunderstood.lThemicroplatecontainssnap-offstrips.Unusedwellsmustbestoredat2°Cto8°Cinthesealedfoilpouchandusedintheframeprovided.lPipettingofsamplesandreagentsmustbedoneasquicklyaspossibleandinthesamesequenceforeachstep.lUsereservoirsonlyforsinglereagents.Thisespeciallyappliestothesubstratereservoirs.Usingareservoirfordispensingasubstratesolutionthathadpreviouslybeenusedfortheconjugatesolutionmayturnsolutioncolored.Donotpourreagentsbackintovialsasreagentcontaminationmayoccur.lMixthecontentsofthemicroplatewellsthoroughlytoensuregoodtestresults.Donotreusemicrowells.lDonotletwellsdryduringassay;addreagentsimmediatelyaftercompletingtherinsingsteps.lAllowthereagentstoreachroomtemperature（21-26°C）beforestartingthetest.Temperaturewillaffecttheabsorbancereadingsoftheassay.However,valuesforthepatientsampleswillnotbeaffected.lNeverpipetbymouthandavoidcontactofreagentsandspecimenswithskinandmucousmembranes.lDonotsmoke,eat,drinkorapplycosmeticsinareaswherespecimensorkitreagentsarehandled.lWeardisposablelatexgloveswhenhand領(lǐng)specimensandreagents.Microbialcontaminationofreagentsorspecimensmaygivefalseresults.lHand領(lǐng)首ldbedoneinaccordancewiththeproceduresdefinedbyanappropriatenationalbiohazardsafetyguidelineorregulation.lDonotusereagentsbeyondexpirydateasshownonthekitlabels.lAllindicatedvolumeshavetobeperformedaccordingtotheprotocol.Optimaltestresultsareonlyobtainedwhenusingcalibratedpipettesandmicrotiterplatereaders.lDonotmixorusecomponentsfromkitswithdifferentlotnumbers.Itisadvisednottoexchangewellsofdifferentplatesevenofthesamelot.Thekitsmayhavebeenshippedorstoredunderdifferentconditionsandthebindingcharacteristicsoftheplatesmayresultslightlydifferent.lAvoidcontactwithStopSolutioncontaining0.5MH2SO4.Itmaycauseskinirritationandburns.lSomereagentscontainProclin,BNDand/orMITaspreservatives.Incaseofcontactwitheyesorskin,flushimmediatelywithwater.lTMBsubstratehasanirritanteffectonskinandmucosa.Incaseofpossiblecontact,washeyeswithanabundantvolumeofwaterandskinwithsoapandabundantwater.Washcontaminatedobjectsbeforereusingthem.Ifinhaled,takethepersontoopenair.lChemicalsandpreparedorusedreagentshavetobetreatedashazardouswasteaccordingtothenationalbiohazardsafetyguidelineorregulation.lForinformationonhazardoussubstancesincludedinthekitpleaserefertoMaterialSafetyDataSheetsMATERIALSPROVIDEDWITHTHEKITMaterialsprovidedwiththekit96determinationsStorageUsermanual1Closureplatemembrane2Sealedbags1Microelisastripplate12-8℃Standard：900pg/ml0.5ml×1bottle2-8℃Standarddiluent1.5ml×1bottle2-8℃HRP-Conjugatereagent6ml×1bottle2-8℃Samplediluent6ml×1bottle2-8℃ChromogenSolutionA6ml×1bottle2-8℃ChromogenSolutionB6ml×1bottle2-8℃StopSolution6ml×1bottle2-8℃washsolution30×20ml×1bottle2-8℃MATERIALSREQUIREDBUTNOTPROVIDEDlMicroplatereadercapableofmeasuringabsorbanceat450nm.lPrecisionpipettestodeliver2mlto1mlvolumes.l100mland1litergraduatedcylinders.lCalibratedadjustableprecisionpipettes,preferablywithdisposableplastictips.（Amanifoldmulti-channelpipetteisdesirableforlargeassays.）lAbsorbentpaper.l37°Cincubator.lDistilledordeionizedwater.lDataanalysisandgraphingsoftware.Graphpaper:linear（Cartesian）,log-logorsemi-log,orlog-logitasdesired.lTubestopreparestandardorsampledilutions.STORAGECONDITIONSuWhenstoredat2°Cto8°Cunopenedreagentswillretainreactivityuntilexpirationdate.uDonotusereagentsbeyondthisdate.Openedreagentsmustbestoredat2°Cto8°C.uMicrotiterwellsmustbestoredat2°Cto8°C.Oncethefoilbaghasbeenopened,care首ldbetakentocloseittightlyagain.uOpenedkitsretainactivityfor8weeksifstoredasdescribedabove.REAGENTPREPARATIONBringallreagentstoroomtemperaturebeforeuseSPECIMENCOLLECTIONANDPREPARATIONSerum-Useaserumseparatortube（SST）andallowsamplestoclotfor30minutesbeforecentrifugationfor15minutesatapproximately1000xg.Removeserumandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Plasma-CollectplasmausingEDTAorheparinasananticoagulant.Centrifugesamplesfor15minutesat1000xgat2-8°Cwithin30minutesofcollection.Storesamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcycles.Cellculturefluidandotherbiologicalfluids-Removeparticulatesbycentrifugationandassayimmediatelyoraliquotandstoresamplesat-20°Cor-80°C.Avoidrepeatedfreeze-thawcyclesASSAYPROCEDUREuGeneralRemarkslAllreagentsandspecimensmustbeallowedtocometoroomtemperaturebeforeuse.Allreagentsmustbemixedwithoutfoaming.lOncethetesthasbeenstarted,allsteps首ldbecompletedwithoutinterruption.lUsenewdisposalplasticpipettetipsforeachstandard,controlorsampleinordertoavoidcrosscontamination.lAbsorbanceisafunctionoftheincubationtimeandtemperature.Beforestartingtheassay,itisrecommendedthatallreagentsareready,capsremoved,allneededwellssecuredinholder,etc.Thiswillensureequalelapsedtimeforeachpipettingstepwithoutinterruption.lAsageneralruletheenzymaticreactionislinearlyproportionaltotimeandtemperature.lDetermineabsorptionwithanELISAreaderat450nmagainst620nmasreference.Ifnoreferencewavelengthisavailable,readonlyat450nm.Iftheextinctionofthehigheststandardexceedsthemeasurementrangeofthephotometer,absorptionmustbemeasuredimmediatelyat405nmagainst620nmasreference.uAssayProcedure1.DiluteandaddsampletoStandard:set10StandardwellsontheELISAplatescoated,addStandard100μltothefirstandthesecondwell,thenaddStandarddilution50μltothefirstandthesecondwell,mix;takeout100μlformthefirstandthesecondwellthenaddittothethirdandtheforthwellseparately.thenaddStandarddilution50μltothethirdandtheforthwell,mix;thentakeout50μlfromthethirdandtheforthwelldiscard,add50μltothefifthandthesixthwell,thenaddStandarddilution50μltothefifthandthesixthwell,mix;takeout50μlfromthefifthandthesixthwellandaddtotheseventhandtheeighthwell,thenaddStandarddilution50μltotheseventhandtheeighthwell,mix;takeout50μlfromtheseventhandtheeighthwellandaddtotheninthandthetenthwell,addStandarddilution50μltotheninthandthetenthwell,mix,takeout50μlfromtheninthandthetenthwelldiscard（addSample50μltoeachwellafterDiluting,（density:600pg/ml，400pg/ml，200pg/ml,100pg/ml，50pg/ml）.50pg/ml100pg/ml600pg/ml200pg/ml900pg/ml400pg/ml2.addsample：Setblankwellsseparately（blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame）.testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl（samplefinaldilutionis5-fold）,addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.3.Incubate:AfterclosingplatewithClosureplatemembrane,incubatefor30minat37℃.4.Configurateliquid:30-foldwashsolutiondiluted30-foldwithdistilledwaterandreserve.5.washing：UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat.6.addenzyme：AddHRP-Conjugatereagent50μltoeachwell,exceptblankwell.7.incubate：Operationwith3.8.washing：Operationwith5.9.color：AddChromogenSolutionA50ulandChromogenSolutionBtoeachwell,evadethelightpreservationfor15minat37℃10.Stopthereaction：AddStopSolution50μltoeachwell,Stopthereaction（thebluecolorchangetoyellowcolor）.11.assay：takeblankwellaszero,Readabsorbanceat450nmafterAddingStopSolutionandwithin15min.CALCULATIONOFRESULTSlCalculatetheaverageabsorbancevaluesforeachsetofstandards,controlsandpatientsamples.lConstructastandardcurvebyplottingthemeanabsorbanceobtainedfromeachstandardagainstits.lconcentrationwithabsorbancevalueonthevertical（Y）axisandconcentrationonthehorizontal（X）axis.lUsingthemeanabsorbancevalueforeachsampledeterminethecorrespondingconcentrationfromthestandardcurve.lAutomatedmethod:TheresultsintheIFUhavebeencalculatedautomaticallyusinga4PL.l（4ParameterLogistics）curvefit.4ParameterLogisticsisthepreferredcalculationmethod.Otherdata.lreductionfunctionsmaygiveslightlydifferentresults.lTheconcentrationofthesamplescanbereaddirectlyfromthisstandardcurve.Sampleswith.lconcentrationshigherthanthatofthehigheststandardhavetobefurtherdiluted.Forthecalculationof.ltheconcentrationsthisdilutionfactorhastobetakenintoaccount.REFERENCESREF:Cat.-No.:/Kat.-Nr.:/No.-Cat.:/Cat.-No.:/N.Cat.:/N.CatLOT:Lot-No.:/Chargen-Bez.:/No.Lot:/Lot-No.:/LoteN.:/Lotton.::No.ofTests:/Kitgre:/Nb.deTests:/No.deDeterm.:/N.deTestes:/Quantitàdeitests::Keepawayfromheatordirectsunlight./VorHitzeunddirekterSonneneinstrahlungschützen./Garderàl’abridelachaleuretdetouteexpositionlumineuse./Manténgasealejadodelcalorolaluzsolardirecta./Manterlongedocalorouluzsolardirecta./Nonesporreairaggisolari.:Readinstructionsbeforeuse./Arbeitsanleitunglesen./Lirelafichetechniqueavantemploi./Lealasinstruccionesantesdeusar./Lerasinstruesantesdeusar./Leggereleistruzioniprimadell’uso.:Storeat:/Lagernbei:/Stockerà:/Almacenea:/Armazenara:/Conservarea:[詳細(xì)]

  2018-09-22 10:00

  產(chǎn)品樣冊(cè)

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• Mouse （VLDL）ELISA Kit

  Mouseverylowdensitylipoprotein(VLDL)ELISAKitFORRESEARCHUSEONLY.NotforclinicaldiagnosisuseCATALOG#:DAG859INTRODUCTION?ThiskitallowsforthedeterminationofVLDLconcentrationsinMouseserum?Detectionofspecies:Mouse?Detectionmedium:serum,cellculturesupernates.?Assayrange：6.0μg/ml-160μg/mlPRINCIPLEOFTESTThekitassayMouseVLDLlevelinthesample，usePurifiedMouseVLDLantibodytocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddVLDLtowells,CombinedVLDLantibodywhichWithHRPlabeled,becomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofMouseVLDLinthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG6342COMPOSITIONOFTHEKIT1washsolution20ml×1bottle7StopSolution6ml×1bottle2HRP-Conjugatereagent6ml×1bottle8Standard（320μg/ml）0.5ml×1bottle3Microelisastripplate12well×8strips9Standarddiluent1.5ml×1bottle4Samplediluent6ml×1bottle10Instruction15ChromogenSolutionA6ml×1bottle11Closureplatemembrane26ChromogenSolutionB6ml×1bottle12Sealedbags1STORAGECONDITIONS?Theunopenedkitshallbestoredat[2-8℃].?Foropenedkitcanbestoredat[2-8℃]forupto1month.Ifnotbeusedrecently,thestandard首ldbekeptin-20℃.WASHINGMETHOD?Manuallywashingmethod:shakeawaytheremainedliquidintheenzymeplates;placesomebibulouspapersonthetest-bed,andflaptheplatesontheupsidedownstrongly.Injectatleast0.35mlafter-dilutionwashingsolutionintothewell,andmarinate1~2minutes.Repeatthisprocessaccordingtoyourrequirements.?Automaticwashingmethod:ifthereisautomaticwashingmachine,it首ldonlybeusedinthetestwhenyouarequitefamiliarwithitsfunctionandperformance.SAMPLEPREPARATION1.extractassoonaspossibleafterSpecimencollection,andaccordingtotherelevantliterature,and首ldbeexperimentassoonaspossibleaftertheextraction.Ifitcan’t,specimencanbekeptin-20℃topreserve,Avoidrepeatedfreeze-thawcycles.Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG63432.Can’tdetectthesamplewhichcontainNaN3,becauseNaN3inhibitsHRPactive.ASSAYPROCEDUREStep1:Diluteandaddsample:DiluteOriginaldensityStandardasfollowtable:Step2:Setblankwellsseparately(blankcomparisonwellsdon’taddsampleandHRP-Conjugatereagent,othereachstepoperationissame).testingsamplewell.addSampledilution40μltotestingsamplewell,thenaddtestingsample10μl(samplefinaldilutionis5-fold),addsampletowells,don’ttouchthewellwallasfaraspossible,andGentlymix.Step3:Incubate:Coverwiththeadhesivestripprovided,incubatefor30minat37℃.Step4:Configurateliquid:Dilutewashsolution30-fold(or20-fold)withdistilledwater.Step5:Washing:Uncovertheadhesivestrip,discardliquid,Pipettewashingbuffertoeverywell,stillfor30sthendrain,repeat5times.Step6:Addenzyme:PipetteHRP-Conjugatereagent50μltoeachwell,exceptblankwell.Step7:Incubate:Operationwith3.Step8:Washing:Operationwith5.160μg/ml5Standard150μlOriginaldensityStandard+150μlStandarddiluent80μg/ml4Standard150μl5Standard+150μlStandarddiluent40μg/ml3Standard150μl4Standard+150μlStandarddiluent20μg/ml2Standard150μl3Standard+150μlStandarddiluent10μg/ml1Standard150μl2Standard+150μlStandarddiluentPhone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG6344Step9:Color:PipetteChromogenSolutionA50ulandChromogenSolutionBtoeachwell,avoidthelightpreservationfor15minat37℃Step10:Stopthereaction:PipetteStopSolution50μltoeachwell,Stopthereaction(thebluechangetoyellow).Step11:Calculate:takeblankwellaszero,Readabsorbanceat450nmafterPipetteingStopSolutionwithin15min.CALCULATIONOFRESULTTakethestandarddensityasthehorizontal,theODvalueforthevertical,drawthestandardcurveongraphpaper,FindoutthecorrespondingdensityaccordingtothesampleODvaluebytheSamplecurve,multipliedbythedilutionmultiple,orcalculatethestraightlineregressionequationofthestandardcurvewiththestandarddensityandtheODvalue,withthesampleODvalueintheequation,calculatethesampledensity,multipliedbythedilutionfactor,theresultisthesampleactualdensity.EXPIRATIONSixmonths[seelabelontheouterboxforthespecificdate].Phone:(612)379-2956Phone:(800)343-7475Fax:(612)656-4400Catalog#:DAG634TTENTION?Thekittakesoutfromtherefrigeration首ldbebalanced15-30minutesintheroomtemperature,ifthecoatedELISAplateshavenotbeenusedupafteropening,theplate首ldbestoredinsealedbag.?washingbufferwillCrystallizationseparation,itcanbeheatedthewaterhelpsdissolvewhendilute.Washingdoesnotaffecttheresult.?addSamplewithsamplerEachstep,Andproofreaditsaccuracyfrequently,avoidstheexperimentalerror.addsamplewithin5min,ifthenumberofsampleismuch,recommendtouseVolley.?ifthetestingmaterialcontentisexcessivelyhigher(ThesampleODisbiggerthanthefirststandardwell),pleasediluteSample(n-fold),Pleasediluenteandmultipliedbythedilutionfactor.（×n×5）.?Closureplatemembraneonlylimitsthedisposableuse,toavoidcross-contamination.?Thesubstrate首ldevadethelighttobepreserved.?Pleaserefertotheuserinstructionstrictly,thetestresultdeterminationmusttakethemicrotiterplatereaderasastandard.?Thepreparationofsamplesandallthereagents首ldrefertoinfectivematerialprocess.?Donotmixreagentswiththosefromotherlots[詳細(xì)]

  2018-10-31 10:00

  產(chǎn)品樣冊(cè)

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• Human 8-OHDG elisa試劑盒

  Human 8-OHDG elisa試劑盒[詳細(xì)]

  2024-09-16 18:47

  期刊論文

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• 人ELISA試劑盒,TPA ELISA Kit

  96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原（TPA）ELISA試劑盒相關(guān)產(chǎn)品：96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原（TPA）ELISA試劑盒IL-10,小鼠白介素-10Elisa試劑盒磺胺二甲基惡唑,標(biāo)準(zhǔn)品JLJL200712人Elisa試劑盒,人ProteinCElisa試劑盒，HumanProteinCELISA試劑盒CAS號(hào):108-69-0,3,5-二,98%人Elisa試劑盒,人CD30Elisa試劑盒，HumanClusterofdifferentiation30,CD30ELISA試劑盒CAS:893-36-7,鹽酸-L-白氨酰-2-萘胺/L-亮氨酰-2-萘胺鹽酸鹽/L-白氨酰-β-萘胺鹽酸鹽/鹽酸-L-亮氨酰-2-萘胺/L（+）-亮氨酰-2-萘基鹽酸氨/L-Leucyl-2-naphthylamidehydrochloride,BR,98%,1克,避光,-20℃96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原（TPA）ELISA試劑盒Humanbacterialvaginosis,BVELISA試劑盒人（BV）kit說明書,細(xì)菌性陰道病Elisa試劑盒CXCR3ELISAKit,大鼠CXC趨化因子受體3Elisa檢測(cè)試劑盒蒙花苷,標(biāo)準(zhǔn)品,含量測(cè)定,20mg,常溫,避光PorcineapoproteinA1,apo-A1ELISA試劑盒豬（apo-A1）kit說明書,載脂蛋白A1Elisa試劑盒大鼠淋巴細(xì)胞因子ELISA試劑盒HumanhepatitisBvirusXinteractingprotein,HBXIPELISAKit人異常凝血酶原（APT）ELISA試劑盒HumanAbnormalprothrombin,APTELISA試劑盒草烏甲素,標(biāo)準(zhǔn)品,含量測(cè)定,50mg,常溫,避光96孔elisa,48孔elisa,HumanTissuePolypeptideAntigen,TPAELISAKit,人組織多肽抗原（TPA）ELISA試劑盒GRP1957,營(yíng)養(yǎng)肉湯,供一般細(xì)菌培養(yǎng)、轉(zhuǎn)種和增菌用,250g小鼠生長(zhǎng)激素釋放多肽（GHRP）ELISA試劑盒HumanMotilin,MTLELISAKitCAS號(hào):4767-3-7,2,2-雙（羥甲基）丙酸,98%[詳細(xì)]

  2018-10-23 10:31

  產(chǎn)品樣冊(cè)

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• [ELISA]CD8分子（CD8）ELISA Kit

  羊CD8分子（CD8）試劑盒使用說明書本試劑盒僅供研究使用。檢測(cè)范圍：96T7IU/ml-240IU/ml試劑盒組成130倍濃縮洗滌液20ml×1瓶7終止液6ml×1瓶2酶標(biāo)試劑6ml×1瓶8標(biāo)準(zhǔn)品（400IU/ml）0.5ml×1瓶3酶標(biāo)包被板12孔×8條9標(biāo)準(zhǔn)品稀釋液1.5ml×1瓶4樣品稀釋液6ml×1瓶10說明書1份5顯色劑A液6ml×1瓶11封板膜2張6顯色劑B液6ml×1/瓶12密封袋1個(gè)標(biāo)本要求1．標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn)，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融2．不能檢測(cè)含NaN3的樣品，因NaN3YZ辣根過氧化物酶的（HRP）活性。羊CD8分子ELISA試劑盒用于測(cè)定羊血清、血漿及相關(guān)液體樣本中CD8分子（CD8）含量。操作步驟1.標(biāo)準(zhǔn)品的稀釋：本試劑盒提供原倍標(biāo)準(zhǔn)品一支，用戶可按照下列圖表在小試管中進(jìn)行稀釋。200IU/ml5號(hào)標(biāo)準(zhǔn)品150μl的原倍標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液100IU/ml4號(hào)標(biāo)準(zhǔn)品150μl的5號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液50IU/ml3號(hào)標(biāo)準(zhǔn)品150μl的4號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液25IU/ml2號(hào)標(biāo)準(zhǔn)品150μl的3號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液12.5IU/ml1號(hào)標(biāo)準(zhǔn)品150μl的2號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液2.加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑，其余各步操作相同）、標(biāo)準(zhǔn)孔、待測(cè)樣品孔。在酶標(biāo)包被板上標(biāo)準(zhǔn)品準(zhǔn)確加樣50μl，待測(cè)樣品孔中先加樣品稀釋液40μl，然后再加待測(cè)樣品10μl（樣品Z終稀釋度為5倍）。加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻。3.溫育：用封板膜封板后置37℃溫育30分鐘。4.配液：將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用5.洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復(fù)5次，拍干。6.加酶：每孔加入酶標(biāo)試劑50μl，空白孔除外。7.溫育：操作同3。8.洗滌：操作同5。9.顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘.10.終止：每孔加終止液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。11.測(cè)定：以空白空調(diào)零，450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）。測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。羊CD8分子ELISA試劑盒注意事項(xiàng)1．試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開封后如未用完，板條應(yīng)裝入密封袋中保存。2．濃洗滌液可能會(huì)有結(jié)晶析出，稀釋時(shí)可在水浴中加溫助溶，洗滌時(shí)不影響結(jié)果。3．各步加樣均應(yīng)使用加樣器，并經(jīng)常校對(duì)其準(zhǔn)確性，以避免試驗(yàn)誤差。一次加樣時(shí)間**控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。4．請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，**做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔**孔的OD值），請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定，計(jì)算時(shí)請(qǐng)Z后乘以總稀釋倍數(shù)（×n×5）。5．封板膜只限一次性使用，以避免交叉污染。6．底物請(qǐng)避光保存。7．嚴(yán)格按照說明書的操作進(jìn)行，試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).8．所有樣品，洗滌液和各種廢棄物都應(yīng)按傳染物處理。9．本試劑不同批號(hào)組分不得混用。10.如與英文說明書有異，以英文說明書為準(zhǔn)。保存條件及有效期1．試劑盒保存：；2-8℃。2．有效期：6個(gè)月羊CD8分子ELISA試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中羊CD8分子（CD8）水平。用純化的羊CD8分子（CD8）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入CD8分子（CD8），再與HRP標(biāo)記的CD8分子（CD8）抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成Z終的黃色。顏色的深淺和樣品中的CD8分子（CD8）呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中羊CD8分子（CD8）濃度。[詳細(xì)]

  2018-11-16 10:02

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• 人黑色素ELISA Kit說明書

  人黑色素ELISA Kit說明書[詳細(xì)]

  2014-08-21 00:00

  安裝說明

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• Kainos FGF-23 ELISA Kit說明書

  KainosLaboratories是日本的生物公司，其生產(chǎn)的FGF-23ELISAKit和ADAMTS13-cleaved廣泛應(yīng)用于各大醫(yī)藥企業(yè)，上海起發(fā)實(shí)驗(yàn)試劑有限公司正式為您帶來KainosLaboratories高品質(zhì)的產(chǎn)品，歡迎來電查詢http://www.kainos.co.jpKainosLaboratories代理KainosLaboratoriesZG代理，KainosLaboratories上海代理，KainosLaboratories總代，KainosLaboratories北京代理ThiskitcapturesADAMTS13-cleavedproductsusingasandwichmethodwiththeanti-GSTmousemonoclonalantibodyandtheperoxidase-conjugatedmousemonoclonalanti-N10antibody.First,theanti-GSTmousemonoclonalantibodyimmobilizedontothemicroplatereactswiththeVWF73substrate（GST-VWF73-His）.Thesampleisthenappliedtothemicroplate,onwhichADAMTS13cleavestheVWF73substrate.Byapplyingthemousemonoclonalanti-N10antibodyconjugatedwithhorseradishperoxidase（HRPconjugatedantibody）,thecleavageproductissandwichedbetweenthetwoantibodies.BecausetheamountofthecleavageproductsdependsontheADAMTS13activity,theamountoftheenzyme-labeledantibodiesthatbindtothecleavageproductsalsoreflectthelevelofADAMTS13activity.Therefore,theplasmaADAMTS13activitycanbedeterminedbycolorimetricallymeasuringtheamountofdetachedoxidized（colored）TMBZusingureahydrogenperoxide（H2O2）and3,3’,5,5’-Tetramethyl-benzidine（TMBZ）asthesubstrates.上海起發(fā)實(shí)驗(yàn)試劑有限公司實(shí)驗(yàn)試劑一站式采購(gòu)服務(wù)商1：強(qiáng)大的進(jìn)口輻射能力，血清、抗體、耗材、大部分限制進(jìn)口品等。2：產(chǎn)品種類齊全，經(jīng)營(yíng)超過700多個(gè)品牌，基本涵蓋所有生物實(shí)驗(yàn)試劑耗材。3：提供加急服務(wù)，貨品一般1-2周到貨。4：富有競(jìng)爭(zhēng)力的價(jià)格優(yōu)勢(shì)，絕大部分價(jià)格有優(yōu)勢(shì)。5：多年積累良好的信譽(yù)，大部分客戶提供貨到付款服務(wù)。客戶包括清華、北大、交大、復(fù)旦、中山等100多所高校，ROCHE，阿斯利康、國(guó)藥、fisher等知名藥企。6：我們還是Santa,AdvancedBiotechnologiesInc,AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc,Genebridege,GlycotopeBiotechnologyGmbH;iduron,InnovativeResearchofAmerica,Ludger,neuroprobe,omicronbio,Polysciences,prospecbi,QA-BIO,quickzyme,RESEARCHDIETS,INC,sterlitech；sysy,TriLinkBioTechnologies,Inc；worthington-biochem,zyagen等幾十家國(guó)外公司授權(quán)代理。7：我們還是invitrogen,qiagen,Tempshield,Inc.am,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā)，歡迎合作。[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊(cè)

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• Protein A ELISA Kit 9333-1說明書

  P/N9333-1RepligenA蛋白ELISA試劑盒ProteinAELISAKitP/N9333-1上海起發(fā)實(shí)驗(yàn)試劑有限公司ProteinAELISAKitP/N9333-1專業(yè)代理，具體產(chǎn)品信息歡迎電詢：4006551678。ProteinAELISAKitHighlightsAlargepercentageoftheworld'scommercialsupplyofantibodyreliesonaRepligenmanufacturedproteinAligandforpurificationandRepligen’sELISAkitforproductrelease.2kitsforthemostaccuratedetectionandquantitationofleachedproteinARecombinantProteinAKitMabSelectSuReKitSuperiorinterandintrakitconsistencyforunparalleledreproducibilityPolyclonalchickencaptureantibodyforgreaterProteinAspecificityBiotinylatedrabbitanti-ProteinAdetectionantibodyforgreatersensitivityMatchedstandardsforthegreatestquantitationaccuracyThreesampleprepprotocolsforsuperiormethodoptimization上海起福生物科技有限公司聯(lián)系人：楊建輝400電話：4006551678辦公電話：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊(cè)

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