資料庫
Phenol extraction of DNA samples
-
本文由 上海士鋒生物科技有限公司 整理匯編
2016-03-17 00:00 598閱讀次數(shù)
文檔僅可預覽首頁內(nèi)容,請下載后查看全文信息!
-
立即下載
Phenol extraction of DNA samples
登錄或新用戶注冊
請用手機微信掃描下方二維碼
快速登錄或注冊新賬號
微信掃碼,手機電腦聯(lián)動
更多資料
-
Phenol extraction of DNA samples
- Phenol extraction of DNA samples[詳細]
-
2016-03-17 00:00
標準
-
ZyGEM BLOOD DNA EXTRACTION 說明書
- 世界ding級實驗材料供應商ZyGEM正式授權上海起發(fā)為其ZG代理,ZyGEM在一直是行業(yè)的標桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務,上海起發(fā)一直秉承為ZG科研客戶帶來**的產(chǎn)品,**的服務,簽約ZyGEM就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務,您的滿意將是我們Zda的收獲ZyGEMZG代理,ZyGEM上海代理,ZyGEM北京代理,ZyGEM廣東代理,ZyGEM江蘇代理ZyGEM湖北代理,ZyGEM天津,ZyGEM黑龍江代理,ZyGEM內(nèi)蒙古代理,ZyGEM吉林代理,ZyGEM福建代理,ZyGEM江蘇代理,ZyGEM浙江代理,ZyGEM四川代理,美國ZyGEM公司是一家生物技術公司,憑借其在新西蘭研發(fā)ZX所擁有的獨特生物資源,以及從極端環(huán)境收集duyi無二微生物群的核心技術,而處于領xian地位。ZyGEM團隊在國際生物技術產(chǎn)業(yè)的商業(yè)、技術與操作上,擁有幾十年豐富的經(jīng)驗。目前該公司推出一系列以來自極端環(huán)境微生物的新蛋白酶催化反應為基礎的DNA提取試劑盒,包括常規(guī)prepGEM、法醫(yī)forensicGEM及牲畜livestockGEM系列產(chǎn)品。BLOODDNAEXTRACTIONPRODUCTCODES100XBP0100500XBP05001000XBP1000開發(fā)一種從血液中提取DNA的快速方法對于有效的研究,醫(yī)學診斷和法醫(yī)學調(diào)查至關重要。鑒于血液提取物的Z終用途范圍廣泛,取樣方法和下游應用可能差異很大。因此,選擇能夠從廣泛的來源材料產(chǎn)生高質(zhì)量DNA的提取方案是至關重要的。PDQeX血液抽取系統(tǒng)采用從全血中提取PCR-readyDNA的多步驟,耗時的過程,并使用ZyGEMPDQeX2400將其變?yōu)楹唵蔚囊徊椒?。一步式封閉式盒式方法既能裂解細胞,又能純化DNA,Zda限度地提高產(chǎn)量并將污染風險降至Zdi。使用侵略性嗜熱性蛋白酶裂解細胞,并且在流出PDQeX柱的出口處的基質(zhì)時純化溶胞產(chǎn)物。DNA不含細胞碎片和YZ劑。通常,來自PDQeX提取的DNA可用于終點PCR,qPCR和STR分析。然而,由于PDQeX程序的Z終高溫步驟,DNA大部分是單鏈的,因此不能用于下一代測序,除非文庫構(gòu)建具有PCR步驟。典型的結(jié)果液體血液提取物使用制備的GEMBloodPDQeX試劑盒從EDTABDVacutainer中提取5μl全血和從指刺中提取新鮮血液。在20μlqPCR反應中使用5μl提取物(總體積100μl)。兩個樣本的圖如下所示。BRCA1,qPCR圖顯示濃度為10ng/μl,2ng/μl,0.4ng/μl,0.08ng/μl的新鮮血液(黑色)和EDTA(藍色)與人類男性標準DNA(紅色)。我們推薦使用PDQeX血液成套工具為液體血液,存儲在卡片和血跡的血液。血液提取物需要將純化聚合物整合到與血液試劑盒一起提供的優(yōu)質(zhì)管中。但是,如果您正在使用抗血小板PCR或STR試劑進行分析,則有可能使用PDQeX標準小柱用于血液檢測,這可能會提供成本較低的選項-特別是如果您的目標DNA數(shù)量足夠稀釋的粗提物可以制成。下面的qPCR圖將20μlqPCR反應與5μl未稀釋的血液提取物或5μl1:10稀釋液進行比較。這些圖顯示了使用PDQeX標準和PDQeX血液獲得的結(jié)果。藍色=PDQeX血液試劑盒,提取未稀釋(效率:102%)品紅=PDQeX血液試劑盒,提取液1:10稀釋(效率:94%)黑色=PDQeX標準試劑盒,提取未稀釋(效率:<50%)紅色=PDQeX血液試劑盒,提取物1:10稀釋(效率=92%)綠色=標準曲線(2ng/μl,0.25ng/μl,0.031ng/μl,0.0039ng/μl)我們公司Zda優(yōu)勢是強大的采購,1:基本什么都能進口,血清,抗體,耗材,還有部分限制進口的,2:貨品全,現(xiàn)經(jīng)營過700多個品牌,基本所有生物試劑耗材都可以進口,特別是冷偏的產(chǎn)品那就更有優(yōu)勢,3:提供加急服務,一般1-2周到貨,超過時限加急費全免4:價格公道,絕大部分價格有優(yōu)勢,當然不能保證1**%產(chǎn)品都是價格Zdi,因為價格Zdi意味著沒有服務.5:良好的信譽,大部分客戶我們提供貨到付款服務,客戶包括清華,北大交大復旦,中山等100多所大學,ROCHE,阿斯利康,國藥,fisher等500多家公司6:我們du家代理的品牌有:AntibodyResearchCorporation,arcticzymes,Biorelevant,AmberGen,Inc.,clemente-associates,clodronateliposomes,ColumbiaBiosciences,enzymeresearch,GeneBridgesGmbH,Genovis,AmberGen,Inc.BiotechnologyGmbH,HaematologicTechnologiesHTIHaemtech,hookelabs,Immudex,InnovativeResearchofAmerica,inspiralis,ListBiologicalLaboratories,Inc.,lumafluor,Microsurfaces,multiplicom,nanotools,Pel-FreezBiologicals,pentapharm,progen,ProteinArk,QA-Bio,Inc,QA-Bio,IncQuickZymeBiosciences,Teknova,TriLinkBioTechnologies,Inc.,ZyagenLaboratories等7:我們還是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā),歡迎合作。[詳細]
-
2018-09-29 10:02
產(chǎn)品樣冊
-
EXTRACTION OF METALS FOR SW846
- EXTRACTION OF METALS FOR SW846[詳細]
-
2024-09-12 18:27
操作手冊
-
Extraction optimization and analysis of monosacchari
- Extraction optimization and analysis of monosacchari[詳細]
-
2024-09-29 01:23
應用文章
-
Viscosity measurement of LDPE samples
- Viscosity measurement of LDPE samples[詳細]
-
2008-06-13 00:00
其它
-
O determination in copper samples
- O determination in copper samples[詳細]
-
2015-06-24 00:00
操作手冊
-
Electrochemical Chloride Extraction from Concrete Br
- Electrochemical Chloride Extraction from Concrete Br[詳細]
-
2014-08-19 00:00
課件
-
METHOD 354 PRESSURIZED FLUID EXTRACTION (PFE)
- METHOD 354 PRESSURIZED FLUID EXTRACTION (PFE)[詳細]
-
2003-12-19 00:00
期刊論文
-
DNA指紋圖譜分析[DNA Fingerprinting ]
- DNA指紋圖譜分析[DNA Fingerprinting ][詳細]
-
2015-09-16 00:00
操作手冊
-
ISOLATION OF THYROXINE FROM AQUEOUS SAMPLES
- ISOLATION OF THYROXINE FROM AQUEOUS SAMPLES[詳細]
-
2006-07-20 00:00
專利
-
ISOLATION OF PHENOLS FROM AQUEOUS SAMPLES
- ISOLATION OF PHENOLS FROM AQUEOUS SAMPLES[詳細]
-
2006-07-20 00:00
期刊論文
-
C, S determination in ore samples
- C, S determination in ore samples[詳細]
-
2015-06-24 00:00
期刊論文
-
O, N determination in steel samples
- O, N determination in steel samples[詳細]
-
2015-06-24 00:00
操作手冊
-
O, N determination in steel samples
- O, N determination in steel samples[詳細]
-
2015-06-24 00:00
期刊論文
-
O, N determination in titanium samples
- SuitableanalyzersnON900nONH2000UsedaccessoriesnInnergraphitecrucibles(90180)nOutergraphitecrucibles(90185)nNickelbasket(90250)orAR2344nSuitablecalibrationmaterial(NISTorother)SettingsnComparatorlevel:20mVnMinimumtime:15secnMaximumtime:3:00minnPostwaiting:30secnBaselinedeviation:200mV;Step:16mV;Time:10secnMode:continuousnOutgasTime:45secPower:6kWnTimePurge:20secStability:45secnIntegrationdelayIRcell:2secTCcell:10secnAnalyse(1)Time:75secPower(1):6kWPower(2):6kWSamplepreparationMakesurethatthesurfaceofthetitaniumisfreefromcontaminations;otherwisecleanthesamplewithacetonep.a.andletdryatatmosphere.ProcedurenPrepareELTRAanalyzer(exchangeanhydrone,sodiumhydroxide,copperoxidewhennecessary),cleanfurnace,sampledropmechanism,electrodetipnRunthreeblankswithemptycruciblesnCalibratetheanalyzerwithsuitablecalibrationmaterial(NISTorother)(1)Filloneemptyinnercrucible(90180)inoneoutercrucible(90185)andplacethemontheelectrodetip,closefurnace(F2Button)(2)Weighcalibrationmaterialandgiveitintoanickelbasket(90250orAR2344)(3)Placenickelbasketwithcalibrationmaterialorsampleinthesampledropmechanismandstartanalysis(F5Button)Afteranalysisgivetheinnercrucibleintowasteandfillinanewone.Theoutercruciblescanbeusedapproximately10times.Repeatsteps(1)(3)atleastthreetimes;Marktheresultsandusethecalibrationfunctioninthesoftware.[詳細]
-
2018-08-16 10:00
產(chǎn)品樣冊
-
C, S determination in wood samples
- SuitableAnalyzersnCS-580seriesnCS-2000(resistancefurnace)UsedAccessoriesn90160DisposableporcelainboatsnSuitablecalibrationmaterialfromNISTorotherSettingsnFurnaceTemperature:1350°CnComperatorlevel:20nminimumanalysistime:60secondsnmaximumanalysistime:180secondsSamplepreparationForbestresultspreparethesampledowntoaparticlesizeabout200m.Drythesampletillmassconstancyat105°C(inminimumfor1Hour).ProcedurenPrepareandcleantheELTRAanalyzer(f.e.exchangeAnhydron,Sodiumhydroxide)andsetthefurnacetemperatureto1350°CnRuninminimumthreewarmupsamples(f.e.ELTRA92511-3020)withamediumsampleweightof200mguntiltheresultsarestablenCalibratethesystemwithasuitablecalibrationmaterial(fromNISTorother):(1)Weighinasampleweightof150mginthe90160porcelainboots(2)Startanalysis(F5Button)(3)LoadthesampleintothefurnaceandwaituntilthePCcalculatesresultsRepeatStep(1)(3)inminimumforthreetimes;marktheresultsandusethecalibrationfunctioninthesoftware[詳細]
-
2018-08-16 10:00
產(chǎn)品樣冊
-
C, S determination in coal samples
- SuitableAnalyzersCS-580seriesCS-2000(resistancefurnace)UsedAccessories90160DisposableporcelainboatsSuitablecalibrationmaterialfromNISTorotherSettingsFurnaceTemperature:1350°CComperatorlevel:20minimumanalysistime:60secondsmaximumanalysistime:180secondsSamplepreparationForbestresultspreparethesampledowntoaparticlesizeabout200μm.Drythesampletillmassconstancyat105°C(inminimumfor1Hour).ProcedurenPrepareandcleantheELTRAanalyzer(f.e.exchangeAnhydron,Sodiumhydroxide)andsetthefurnacetemperatureto1350°CnRuninminimumthreewarmupsamples(f.e.ELTRA92511-3020)withamediumsampleweightof200mguntiltheresultsarestablenCalibratethesystemwithasuitablecalibrationmaterial(fromNISTorother):(1)Weighinasampleweightof200mginthe90160porcelainboots(2)Startanalysis(F5Button)(3)LoadthesampleintothefurnaceandwaituntilthePCcalculatesresultsRepeatStep(1)(3)inminimumforthreetimes;marktheresultsandusethecalibrationfunctioninthesoftware->Analyzetheunknownsamples.[詳細]
-
2018-08-16 10:00
產(chǎn)品樣冊
-
Determination of agmatine in biological samples by c
- Determination of agmatine in biological samples by c[詳細]
-
2024-09-18 18:08
課件
-
Determination of agmatine in biological samples by c
- Determination of agmatine in biological samples by c[詳細]
-
2024-09-28 00:08
專利
-
ISOLATION OF THIAMINE FROM AQUEOUS SAMPLES
- ISOLATION OF THIAMINE FROM AQUEOUS SAMPLES[詳細]
-
2024-09-24 01:13
報價單
Copyright 2004-2026 yiqi.com All Rights Reserved , 未經(jīng)書面授權 , 頁面內(nèi)容不得以任何形式進行復制
參與評論
登錄后參與評論