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• BioCheck新產(chǎn)品BC-1303 說明書

  世界ding級實(shí)驗(yàn)材料供應(yīng)商BioCheck正式授權(quán)上海起發(fā)為其ZG代理，BioCheck在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來**的產(chǎn)品，**的服務(wù)，簽約BioCheck就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck廣東代理，BioCheck江蘇代理BioCheck湖北代理,BioCheck天津,BioCheck黑龍江代理,BioCheck內(nèi)蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江蘇代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由創(chuàng)始人Dr.JohnChen,Medix創(chuàng)立,是一家抓也提供腫瘤標(biāo)志物，心肌標(biāo)志物，激素類Z高性價(jià)比的抗體的公司。簡要原理利用競爭酶聯(lián)免疫方法，預(yù)先在微孔中包被羊抗兔抗體，實(shí)驗(yàn)時(shí)先后加入氯霉素標(biāo)準(zhǔn)品或待測樣本，氯霉素酶標(biāo)抗原和兔抗氯霉素抗體。經(jīng)過室溫溫育，反應(yīng)液中的兔抗氯霉素抗體與微孔板上的羊抗兔抗體結(jié)合，待測樣品中的抗原與氯霉素酶標(biāo)抗原競爭微孔板上的兔抗氯霉素抗體。洗滌后，沒有與抗體結(jié)合的待測樣品中的抗原或酶標(biāo)抗原被洗去，再加入反應(yīng)底物，結(jié)合的酶標(biāo)抗原的酶將底物轉(zhuǎn)化為藍(lán)色產(chǎn)物，加入終止液后顏色由藍(lán)色變?yōu)辄S色。反應(yīng)完成后，樣品中氯霉素含量越多，反應(yīng)呈色就越淺；反之，樣品中氯霉素含量越少，則呈色越深。利用標(biāo)準(zhǔn)曲線可計(jì)算出樣品中氯霉素含量。技術(shù)參數(shù)檢測蜂蜜、蛋類、牛奶、奶粉、水產(chǎn)品、動(dòng)物組織(肌肉、肝臟等)、飼料、血清、血漿及尿液樣本中存在的氯霉素，定量限可達(dá)0.025ppb。No.樣品檢測下限1蜂蜜0.02ppb(0.02ng/g)2蛋類0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5蝦，魚及肉類0.08ppb(0.08ng/g)6飼料0.08ppb(0.08ng/g)7血清/血漿0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反應(yīng)名稱百分比氯霉素堿0.4%甲基氯霉素<0.04%回收率No.樣品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，蝦，魚及肉類95%~120%4飼料95%~120%5血清/血漿90%~120%6尿液1**%~130%PD-L1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1303EnzymeImmunoassayfortheQuantitativeDeterminationofPD-L1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammeddeathreceptor-ligand1(PD-L1,B7-H1,CD274)isabiomarkerfromtheB7familythatisexpressedonavarietyofcellsandupregulatedinresponsetopro-inflammatorycytokinessuchasinterferongamma1,2,3.PD-L1interactswithPD-1,aco-receptorexpressedbyexhaustedTcells,toencourageanimmunosuppressivetumormicroenvironmentbydecreasingTcellreceptormediatedproliferationandcytokineproduction4,5.ThePD-1/PD-L1interactionfunctionsasanimmunecheckpointinaprocessknownasimmunoeditingwherethehostimmunesystemeliminateshighlyimmunogenictumorswhileallowinglessimmunogenictumortoevadeit2,6.Multiplesolidtumortypesincludingmelanoma,renalcellcarcinoma,non-smallcelllungcancer,ovarian,andcolorectalcancerutilizethisPD-1/PD-L1immunoeditingmechanism2.CurrenttreatmenthasfocusedonblockingthePD-1/PD-L1interactiontoreducetumorevasionbyinhibitingPD-1,withnewfocusonPD-L1aswell1,2.PRINCIPLEOFTHEASSAYThePD-L1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-L1molecule.Onemousemonoclonalanti-PD-L1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-L1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-L1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-L1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-L120ng/mlPD-L1Standard(0.5mL/vial)20ng/mLPD-L1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-L1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute20ng/mLstandardto5ng/mL.Preparetwo-foldserialdilutionsofthe5ng/mLStandardwithStandard/SampleDiluent:5ng/mL:0.15mLof20ng/mL+0.45mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0.078ng/mL:0.25mLof0.156ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-L1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-L1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-L1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-L1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-L1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-L1Absorbance(ng/ml)(450nm)00.0420.0780.1410.1560.2360.3130.4150.6250.7261.251.2832.52.10053.053nm3.53(4502.52Absorbance1.510.50012345PD-L1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-L1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.02ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-L1(ng/mL)1.53.07.4S.D.0.040.050.18C.V.(%)2.4%1.6%2.4%2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-L1(ng/mL)1.63.07.4S.D.0.040.120.26C.V.(%)2.5%4.1%3.5%RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-L1levelsandassayedinduplicate.Themeanrecoverywas90%.SampleEXPECTEDOBSERVED%RECOVERY[PD-L1][PD-L1](ng/mL)(ng/mL)121.890%254.692%3108.989%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas110.3%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:41.61.6N/A1:81.7106.3%1:161.7106.3%1:321.8112.5%Mean=108.4%2.1:43.73.7N/A1:84.0108.1%1:164.0108.1%1:324.1110.8%Mean=109.0%3.1:47.47.4N/A1:88.3112.2%1:168.6116.2%1:328.3112.2%Mean=113.5%REFERENCESHerbst,RoyS.,etal."Predictivecorrelatesofresponsetotheanti-PD-L1antibodyMPDL3280Aincancerpatients."Nature515.7528(2014):563.Patel,SandipPravin,andRazelleKurzrock."PD-L1expressionasapredictivebiomarkerincancerimmunotherapy."Molecularcancertherapeutics14.4(2015):847-856.Topalian,SuzanneL.,CharlesG.Drake,andDrewM.Pardoll."TargetingthePD-1/B7-H1(PD-L1)pathwaytoactivateanti-tumorimmunity."Currentopinioninimmunology24.2(2012):207-212.Blank,Christian,andAndreasMackensen."ContributionofthePD-L1/PD-1pathwaytoT-cellexhaustion:anupdateonimplicationsforchronicinfectionsandtumorevasion."Cancerimmunology,immunotherapy56.5(2007):739-745.Barber,DanielL.,etal."RestoringfunctioninexhaustedCD8Tcellsduringchronicviralinfection."Nature439.7077(2006):682.Teng,MicheleWL,etal."ClassifyingcancersbasedonT-cellinfiltrationandPD-L1."Cancerresearch75.11(2015):2139-2145.部分Zxin產(chǎn)品如下：貨號(hào)品名Tests/Kit規(guī)格品牌70748MouseMonoclonalanti-humanPD-1(Capture)Purified0.1/0.5/1mgBioCheck70749MouseMonoclonalanti-humanPD-1(Capture)Purified0.1/0.5/1mgBioCheck70750MouseMonoclonalanti-humanPD-1(Detection)Purified0.1/0.5/1mgBioCheck70751MouseMonoclonalanti-humanPD-L1(Capture)Purified0.1/0.5/1mgBioCheck70752MouseMonoclonalanti-humanPD-L1(Capture)Purified0.1/0.5/1mgBioCheck70753MouseMonoclonalanti-humanPD-L1(Detection)Purified0.1/0.5/1mgBioCheck70745Mousemonoclonalanti-humanLp-PLA2(Capture)Purified0.5mgBioCheck70746Mousemonoclonalanti-humanLp-PLA2(Detection)Purified0.5mgBioCheck70747Mousemonoclonalanti-humanLp-PLA2(Detection)Purified0.5mgBioCheck我們公司Zda優(yōu)勢是強(qiáng)大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都是價(jià)格Zdi,因?yàn)閮r(jià)格Zdi意味著沒有服務(wù).5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們du家代理的品牌有：AntibodyResearchCorporation，arcticzymes，Biorelevant，AmberGen,Inc.，clemente-associates，clodronateliposomes，ColumbiaBiosciences，enzymeresearch，GeneBridgesGmbH，Genovis，AmberGen,Inc.BiotechnologyGmbH，HaematologicTechnologiesHTIHaemtech，hookelabs，Immudex，InnovativeResearchofAmerica，inspiralis，ListBiologicalLaboratories,Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-FreezBiologicals，pentapharm，progen，ProteinArk，QA-Bio,Inc，QA-Bio,IncQuickZymeBiosciences，Teknova，TriLinkBioTechnologies,Inc.，ZyagenLaboratories等7：我們還是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā)，歡迎合作。[詳細(xì)]

  2018-09-29 10:02

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• BioCheck新產(chǎn)品BC-1301 說明書

  世界ding級實(shí)驗(yàn)材料供應(yīng)商BioCheck正式授權(quán)上海起發(fā)為其ZG代理，BioCheck在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來**的產(chǎn)品，**的服務(wù)，簽約BioCheck就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck廣東代理，BioCheck江蘇代理BioCheck湖北代理,BioCheck天津,BioCheck黑龍江代理,BioCheck內(nèi)蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江蘇代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由創(chuàng)始人Dr.JohnChen,Medix創(chuàng)立,是一家抓也提供腫瘤標(biāo)志物，心肌標(biāo)志物，激素類Z高性價(jià)比的抗體的公司。簡要原理利用競爭酶聯(lián)免疫方法，預(yù)先在微孔中包被羊抗兔抗體，實(shí)驗(yàn)時(shí)先后加入氯霉素標(biāo)準(zhǔn)品或待測樣本，氯霉素酶標(biāo)抗原和兔抗氯霉素抗體。經(jīng)過室溫溫育，反應(yīng)液中的兔抗氯霉素抗體與微孔板上的羊抗兔抗體結(jié)合，待測樣品中的抗原與氯霉素酶標(biāo)抗原競爭微孔板上的兔抗氯霉素抗體。洗滌后，沒有與抗體結(jié)合的待測樣品中的抗原或酶標(biāo)抗原被洗去，再加入反應(yīng)底物，結(jié)合的酶標(biāo)抗原的酶將底物轉(zhuǎn)化為藍(lán)色產(chǎn)物，加入終止液后顏色由藍(lán)色變?yōu)辄S色。反應(yīng)完成后，樣品中氯霉素含量越多，反應(yīng)呈色就越淺；反之，樣品中氯霉素含量越少，則呈色越深。利用標(biāo)準(zhǔn)曲線可計(jì)算出樣品中氯霉素含量。技術(shù)參數(shù)檢測蜂蜜、蛋類、牛奶、奶粉、水產(chǎn)品、動(dòng)物組織(肌肉、肝臟等)、飼料、血清、血漿及尿液樣本中存在的氯霉素，定量限可達(dá)0.025ppb。No.樣品檢測下限1蜂蜜0.02ppb(0.02ng/g)2蛋類0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5蝦，魚及肉類0.08ppb(0.08ng/g)6飼料0.08ppb(0.08ng/g)7血清/血漿0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反應(yīng)名稱百分比氯霉素堿0.4%甲基氯霉素<0.04%回收率No.樣品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，蝦，魚及肉類95%~120%4飼料95%~120%5血清/血漿90%~120%6尿液1**%~130%PD-1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1301EnzymeImmunoassayfortheQuantitativeDeterminationofPD-1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammedcelldeathprotein1,(PD-1,CD279,PDCD1),isacellsurfacereceptorthatiscriticalfortheregulationofTcellinflammatoryactivityandmaintainsperipheraltoleranceintheimmunesystem(1).WithanextracellularIgVdomain,atransmembranedomain,andanintracellulartailwithtwophosphorylationsites(SHP-1andSHP-2),PD-1isabletointeractwithligandsPD-L1andPD-L2todown-regulatetheimmunesystemandsuppressTcellactivity(2).Uponantigenrecognition,anactivatedTcellexpressesPD-1onitssurfaceandproducesinterferons,whichinducesexpressionofPD-L1,therebypromotingapoptosisorcelldeath.(3)Howeverwhenhijackedbytumorcells,aberrantexpressionofPD-1ligandsinhibitsimmune-modulatoryTcellactivation,leadingtodiseaseprogression(4).ElevatedlevelsofPD-1inserumandplasmahavebeenassociatedwithrheumatoidarthritisandskinsclerosis(5,6).Also,PD-1ispredominantlyexpressedbytumorinfiltratingTcells(7).FurtherresearchimpliesthatusingmonoclonalantibodiestotargetthePD-1immunologiccheckpointhascontributedtobreakthroughprogressinunderstandingandtreatingcancerssuchasmelanomaandothervariousnon-smallcelllungcancer(4).PRINCIPLEOFTHEASSAYThePD-1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-1molecule.Onemousemonoclonalanti-PD-1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-150ng/mlPD-1Standard(0.5mL/vial)50ng/mLPD-1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute50ng/mLto10ng/mLwithStandard/Samplediluent.Preparetwo-foldserialdilutionsofthe10ng/mLStandardwithStandard/SampleDiluent:10ng/mL:0.12mLof50ng/mL+0.48mLofStandard/SampleDiluent5ng/mL:0.25mLof10ng/mL+0.25mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-1Absorbance(ng/ml)(450nm)00.0890.1560.1380.3130.1900.6250.2841.250.4812.50.85151.581102.776nm32.5(4502Absorbance1.510.500246810PD-1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.15ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-1(ng/mL)8.413.222.9S.D.0.61.00.9C.V.(%)7.0%7.2%3.8%.2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-1(ng/mL)9.113.525.3S.D.0.81.10.9C.V.(%)9.2%7.93.7RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-1levelsandassayedinduplicate.Themeanrecoverywas105.3%.PAIREXPECTEDOBSERVED%RECOVERYNUMBER[PD-1][PD-1](ng/mL)(ng/mL)188.8110.0%212.513.1104.8%32525.3101.2%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas99.6%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:210.1114.8%1:48.88.8N/A1:87.888.6%Mean=101.7%2.1:214.5110.6%1:413.113.1N/A1:811.688.5%Mean=99.6%3.1:226.5104.7%1:425.325.3N/A1:823.090.0%Mean=97.4%REFERENCESFife,B.T.,&Pauken,K.E.(2011).TheroleofthePD-1pathwayinautoimmunityandperipheraltolerance.AnnalsoftheNewYorkAcademyofSciences,1217(1),45-59.Riella,L.V.,Paterson,A.M.,Sharpe,A.H.,&Chandraker,A.(2012).RoleofthePD-1PathwayintheImmuneResponse.AmericanJournalofTransplantation:OfficialJournaloftheAmericanSocietyofTransplantationandtheAmericanSocietyofTransplantSurgeons,12(10),25752587.Zak,Krzysztof&Kitel,Radosaw&Przetocka,Sara&Golik,Przemysaw&Guzik,Katarzyna&Musielak,Bogdan&Dm領(lǐng),Alexander&Dubin,Grzegorz&Holak,Tad.(2015).StructureoftheComplexofHumanProgrammedDeath1,PD-1,andItsLigandPD-L1.Structure.23..10.1016/j.str.2015.09.010.ZoranGatalica,CarrieSnyder,ToddManey,AnatoleGhazalpour,DanielHolterman,NianqingXiao,PeggyOverberg,IngaRose,GargiD.Basu,SemirVranic,HenryT.Lynch,DanielD.VonHoffandOmidHamid.ProgrammedCellDeath1(PD-1)andItsLigand(PD-L1)inCommonCancersandTheirCorrelationwithMolecularCancerType.CancerEpidemiolBiomarkersPrev.December12014(23)(12)2965-2970.Greisen,S.,Rasmussen,T.,Stengaard-Pedersen,K.,Hetland,M.,Hrslev-Petersen,K.,Hvid,M.,&Deleuran,B.(2013).Increasedsolubleprogrammeddeath-1(sPD-1)isassociatedwithdiseaseactivityandradiographicprogressioninearlyrheumatoidarthritis.ScandinavianJournalofRheumatology,43(2),101-108.Yanaba,K.,Hayashi,M.,Yoshihara,Y.,&Nakagawa,H.(2016).Serumlevelsofsolubleprogrammeddeath-1andprogrammeddeathligand-1insystemicsclerosis:Associationwithextentofskinsclerosis.TheJournalofDermatology,43(8),954-957.Ahmadzadeh,M.,Johnson,L.A.,Heemskerk,B.,Wunderlich,J.R.,Dudley,M.E.,White,D.E.,&Rosenberg,S.A.(2009).TumorantigenspecificCD8TcellsinfiltratingthetumorexpresshighlevelsofPD-1andarefunctionallyimpaired.Blood,114(8),15371544.部分產(chǎn)品Zxin報(bào)價(jià)如下：貨號(hào)品名規(guī)格價(jià)格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我們公司Zda優(yōu)勢是強(qiáng)大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都有價(jià)格優(yōu)勢，5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們還是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......幾十家國外公司授權(quán)代理7：我們還從事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批發(fā)[詳細(xì)]

  2018-09-29 10:02

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• DLD 新產(chǎn)品EA611/96 說明書

  世界ding級實(shí)驗(yàn)材料供應(yīng)商DLDDiagnostikaGmbH正式授權(quán)上海起發(fā)為其ZG代理，DLDDiagnostikaGmbH在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來**的產(chǎn)品，**的服務(wù)，簽約DLDDiagnostikaGmbH就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲DLDDiagnostikaGmbHZG代理,DLDDiagnostikaGmbH上海代理，DLDDiagnostikaGmbH北京代理，DLDDiagnostikaGmbH廣東代理，DLDDiagnostikaGmbH江蘇代理DLDDiagnostikaGmbH湖北代理,DLDDiagnostikaGmbH天津,DLDDiagnostikaGmbH黑龍江代理,DLDDiagnostikaGmbH內(nèi)蒙古代理,DLDDiagnostikaGmbH吉林代理,DLDDiagnostikaGmbH福建代理,DLDDiagnostikaGmbH江蘇代理,DLDDiagnostikaGmbH浙江代理,DLDDiagnostikaGmbH四川代理,DLD開發(fā)，銷售和提供科學(xué)服務(wù)，用于體外診斷的高質(zhì)量創(chuàng)新測試。產(chǎn)品范圍包括自身免疫性疾病，心血管疾病，癌癥疾病和腎功能不全等所謂的“利基測試”，DLD是研究與常規(guī)實(shí)驗(yàn)室之間的協(xié)調(diào)點(diǎn)。DLDdevelops,marketsandprovidesscientificserviceforhighqualityinnovativetestsforin-vitro-diagnosis.Theproductsrangeincludesso-callednichetests"forautoimmunediseases,cardiovasculardiseases,cancerdiseasesandrenaldysfunction.DLDisthecoordinatingpointbetweenresearchandroutinelab.Calretinin-ELISACatalog-No.EA611/96FormatMicrotiterplate(12x8determinations,singlebreakapartwells;coatedwithpolyclonalcalretininantibodiesProcedureSamplevolume:15碌lpatientserumorplasma1.SamplePreparation:60minshakingatroomtemperature2.ELISA:5hoursincubationatroomtemperatureMeasurementinELISAreaderat450nm(620nmreferencewavelength)6Standardswithrange:0.25ng/ml-4.0ng/ml2Kitcontrols,lyophilizedWashbuffer50xconcentratedAllotherreagentsreadyforuseClinicalFieldsOncology,cancerscreening,occupationaldiseasesDiseasesMesotheliomaSensitivity0.05ng/mlCharacteristicsSandwichELISAwithmicrotiterplateformateHighlyspecificandsensitiverabbitanti-calretininantibodyGoodlinearitybetween0.25-4.0ng/mlEasytohandle,totalassaytimeis6hoursSuitablefortestinglargeseriesofserumorplasmasamplesValidatedinlargercase-controlstudiesInvolvedinprospectivestudies部分產(chǎn)品2017Zxin報(bào)價(jià)如下:貨號(hào)品名規(guī)格目錄價(jià)品牌EA110/96ACHRABAssayELISA12x811975dld-diagnostikaEA611/96CalretininELISA12x89879dld-diagnostikaEA112/9621-HydroxylaseAntibodyELISA12x811975dld-diagnostikaEA300/9625-OHVitaminDELISA12x85888dld-diagnostikaEA601/48TitinAntibodyELISA6x84191dld-diagnostikaEA111/96Aquaporin-4(AQP4)AntibodyELISA12x813871dld-diagnostikaEA201/96ADMAELISA12x89879dld-diagnostikaEA212/96ADMAFastELISA12x89879dld-diagnostikaEA209/96ADMAHighSensitiveELISA12x811975dld-diagnostikaEA207/192ADMAArginineELISA24x813871dld-diagnostikaEA205/96HomoarginineELISA12x813871dld-diagnostikaEA203/96SDMA-ELISA12x89879dld-diagnostikaEA101/96TSHReceptorAntibodyELISA12x84590dld-diagnostikaEA104/96GAD6ntibodyELISA12x86985dld-diagnostikaEA114/96IA2AntibodyELISA12x86985dld-diagnostikaEA102/96ZincTransporter8(ZnT8)AntibodyELISA12x89879dld-diagnostikaEA602/96SerotoninELISA12x83992dld-diagnostikaEA630/96SerotoninHighSensitiveELISA12x84191dld-diagnostikaEA614/192Nor-/MetanephrineinUrineELISA2x964890dld-diagnostikaEA612/192Nor-/MetanephrineinPlasmaELISA2x965089dld-diagnostikaEA604/96AdrenalineELISA12x84590dld-diagnostikaEA610/96NoradrenalineELISA12x84590dld-diagnostikaEA608/96DopamineELISA12x84590dld-diagnostikaEA631/192Noradrenaline/DopamineHighSensitiveELISA2x969879dld-diagnostikaEA632/96AdrenalineHighSensitiveELISA12x87883dld-diagnostikaEA633/96NoradrenalineHighSensitiveELISA12x87883dld-diagnostikaEA634/96DopamineHighSensitiveELISA12x87883dld-diagnostikaEA613/192BI-CATELISA(Adrenaline,Noradrenaline)inUrine&Plasma2x965688dld-diagnostikaEA603/288CATELISA(Adrenaline,Noradrenaline,Dopamine)inUrine&Plasma3x966586dld-diagnostikaEA213/96HistamineELISA12x83892dld-diagnostikaEA208/961-MethylhistamineELISA12x84690dld-diagnostika我們公司Zda優(yōu)勢是強(qiáng)大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都是價(jià)格Zdi,因?yàn)閮r(jià)格Zdi意味著沒有服務(wù).5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們還是Santa,AdvancedBiotechnologiesInc,DLDDiagnostikaGmbH,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.Dako,Inc,GePromegaridege,DLDDiagnostikaGmbHBiotechnologyGmbH;iduron,InnovativeResearchofAmerica,Ludger,neuroprobe,DLDDiagnostikaGmbH,Polysciences,prospecbi,QA-BIO,quickzyme,FULLERLABS,INC,sterlitech；sysy,TriLinkBioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。7：我們還是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā)，歡迎合作。[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊

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• BioCheck新產(chǎn)品貨號(hào)70747說明書

  世界ding級實(shí)驗(yàn)材料供應(yīng)商BioCheck正式授權(quán)上海起發(fā)為其ZG代理，BioCheck在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來**的產(chǎn)品，**的服務(wù)，簽約BioCheck就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck廣東代理，BioCheck江蘇代理BioCheck湖北代理,BioCheck天津,BioCheck黑龍江代理,BioCheck內(nèi)蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江蘇代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由創(chuàng)始人Dr.JohnChen,Medix創(chuàng)立,是一家抓也提供腫瘤標(biāo)志物，心肌標(biāo)志物，激素類Z高性價(jià)比的抗體的公司。簡要原理利用競爭酶聯(lián)免疫方法，預(yù)先在微孔中包被羊抗兔抗體，實(shí)驗(yàn)時(shí)先后加入氯霉素標(biāo)準(zhǔn)品或待測樣本，氯霉素酶標(biāo)抗原和兔抗氯霉素抗體。經(jīng)過室溫溫育，反應(yīng)液中的兔抗氯霉素抗體與微孔板上的羊抗兔抗體結(jié)合，待測樣品中的抗原與氯霉素酶標(biāo)抗原競爭微孔板上的兔抗氯霉素抗體。洗滌后，沒有與抗體結(jié)合的待測樣品中的抗原或酶標(biāo)抗原被洗去，再加入反應(yīng)底物，結(jié)合的酶標(biāo)抗原的酶將底物轉(zhuǎn)化為藍(lán)色產(chǎn)物，加入終止液后顏色由藍(lán)色變?yōu)辄S色。反應(yīng)完成后，樣品中氯霉素含量越多，反應(yīng)呈色就越淺；反之，樣品中氯霉素含量越少，則呈色越深。利用標(biāo)準(zhǔn)曲線可計(jì)算出樣品中氯霉素含量。技術(shù)參數(shù)檢測蜂蜜、蛋類、牛奶、奶粉、水產(chǎn)品、動(dòng)物組織(肌肉、肝臟等)、飼料、血清、血漿及尿液樣本中存在的氯霉素，定量限可達(dá)0.025ppb。No.樣品檢測下限1蜂蜜0.02ppb(0.02ng/g)2蛋類0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5蝦，魚及肉類0.08ppb(0.08ng/g)6飼料0.08ppb(0.08ng/g)7血清/血漿0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反應(yīng)名稱百分比氯霉素堿0.4%甲基氯霉素<0.04%回收率No.樣品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，蝦，魚及肉類95%~120%4飼料95%~120%5血清/血漿90%~120%6尿液1**%~130%ProductName:MouseMonoclonalAnti-HumanLipoproteinAssociatedPhospholipaseA2(Lp-PLA2)CatalogNumber:70747Subclass:IgG1ProductForm:PBS,pH7.4with0.09%sodiumazideStorage:At2-8C.DoNotFreeze.Precipitationmayoccuruponfreezing.SuggestedUse:Forenzymeconjugation(detection)inthehumanLpPLA2immunoassayPairingInformation:ThisantibodypairswithMouseMonoclonalAnti-HumanLp-PLA2PartNo.70745部分產(chǎn)品Zxin報(bào)價(jià)如下：貨號(hào)品名規(guī)格價(jià)格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我們公司Zda優(yōu)勢是強(qiáng)大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都有價(jià)格優(yōu)勢，5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們還是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......幾十家國外公司授權(quán)代理7：我們還從事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批發(fā)[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊

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• BioCheck新產(chǎn)品貨號(hào)70746說明書

  世界ding級實(shí)驗(yàn)材料供應(yīng)商BioCheck正式授權(quán)上海起發(fā)為其ZG代理，BioCheck在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來**的產(chǎn)品，**的服務(wù)，簽約BioCheck就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck廣東代理，BioCheck江蘇代理BioCheck湖北代理,BioCheck天津,BioCheck黑龍江代理,BioCheck內(nèi)蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江蘇代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由創(chuàng)始人Dr.JohnChen,Medix創(chuàng)立,是一家抓也提供腫瘤標(biāo)志物，心肌標(biāo)志物，激素類Z高性價(jià)比的抗體的公司。簡要原理利用競爭酶聯(lián)免疫方法，預(yù)先在微孔中包被羊抗兔抗體，實(shí)驗(yàn)時(shí)先后加入氯霉素標(biāo)準(zhǔn)品或待測樣本，氯霉素酶標(biāo)抗原和兔抗氯霉素抗體。經(jīng)過室溫溫育，反應(yīng)液中的兔抗氯霉素抗體與微孔板上的羊抗兔抗體結(jié)合，待測樣品中的抗原與氯霉素酶標(biāo)抗原競爭微孔板上的兔抗氯霉素抗體。洗滌后，沒有與抗體結(jié)合的待測樣品中的抗原或酶標(biāo)抗原被洗去，再加入反應(yīng)底物，結(jié)合的酶標(biāo)抗原的酶將底物轉(zhuǎn)化為藍(lán)色產(chǎn)物，加入終止液后顏色由藍(lán)色變?yōu)辄S色。反應(yīng)完成后，樣品中氯霉素含量越多，反應(yīng)呈色就越淺；反之，樣品中氯霉素含量越少，則呈色越深。利用標(biāo)準(zhǔn)曲線可計(jì)算出樣品中氯霉素含量。技術(shù)參數(shù)檢測蜂蜜、蛋類、牛奶、奶粉、水產(chǎn)品、動(dòng)物組織(肌肉、肝臟等)、飼料、血清、血漿及尿液樣本中存在的氯霉素，定量限可達(dá)0.025ppb。No.樣品檢測下限1蜂蜜0.02ppb(0.02ng/g)2蛋類0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5蝦，魚及肉類0.08ppb(0.08ng/g)6飼料0.08ppb(0.08ng/g)7血清/血漿0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反應(yīng)名稱百分比氯霉素堿0.4%甲基氯霉素<0.04%回收率No.樣品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，蝦，魚及肉類95%~120%4飼料95%~120%5血清/血漿90%~120%6尿液1**%~130%ProductName:MouseMonoclonalAnti-HumanLipoproteinAssociatedPhospholipaseA2(Lp-PLA2)CatalogNumber:70746Subclass:IgG1ProductForm:PBS,pH7.4with0.09%sodiumazideStorage:At2-8C.DoNotFreeze.Precipitationmayoccuruponfreezing.SuggestedUse:Forenzymeconjugation(detection)inthehumanLpPLA2immunoassay.PairingInformation:ThisantibodypairswithMouseMonoclonalAnti-HumanLp-PLA2PartNo.70745部分產(chǎn)品Zxin報(bào)價(jià)如下：貨號(hào)品名規(guī)格價(jià)格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我們公司Zda優(yōu)勢是強(qiáng)大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都有價(jià)格優(yōu)勢，5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們還是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......幾十家國外公司授權(quán)代理7：我們還從事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批發(fā)[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊

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• BioCheck新產(chǎn)品貨號(hào)70745說明書

  世界ding級實(shí)驗(yàn)材料供應(yīng)商BioCheck正式授權(quán)上海起發(fā)為其ZG代理，BioCheck在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來**的產(chǎn)品，**的服務(wù)，簽約BioCheck就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck廣東代理，BioCheck江蘇代理BioCheck湖北代理,BioCheck天津,BioCheck黑龍江代理,BioCheck內(nèi)蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江蘇代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由創(chuàng)始人Dr.JohnChen,Medix創(chuàng)立,是一家抓也提供腫瘤標(biāo)志物，心肌標(biāo)志物，激素類Z高性價(jià)比的抗體的公司。簡要原理利用競爭酶聯(lián)免疫方法，預(yù)先在微孔中包被羊抗兔抗體，實(shí)驗(yàn)時(shí)先后加入氯霉素標(biāo)準(zhǔn)品或待測樣本，氯霉素酶標(biāo)抗原和兔抗氯霉素抗體。經(jīng)過室溫溫育，反應(yīng)液中的兔抗氯霉素抗體與微孔板上的羊抗兔抗體結(jié)合，待測樣品中的抗原與氯霉素酶標(biāo)抗原競爭微孔板上的兔抗氯霉素抗體。洗滌后，沒有與抗體結(jié)合的待測樣品中的抗原或酶標(biāo)抗原被洗去，再加入反應(yīng)底物，結(jié)合的酶標(biāo)抗原的酶將底物轉(zhuǎn)化為藍(lán)色產(chǎn)物，加入終止液后顏色由藍(lán)色變?yōu)辄S色。反應(yīng)完成后，樣品中氯霉素含量越多，反應(yīng)呈色就越淺；反之，樣品中氯霉素含量越少，則呈色越深。利用標(biāo)準(zhǔn)曲線可計(jì)算出樣品中氯霉素含量。技術(shù)參數(shù)檢測蜂蜜、蛋類、牛奶、奶粉、水產(chǎn)品、動(dòng)物組織(肌肉、肝臟等)、飼料、血清、血漿及尿液樣本中存在的氯霉素，定量限可達(dá)0.025ppb。No.樣品檢測下限1蜂蜜0.02ppb(0.02ng/g)2蛋類0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5蝦，魚及肉類0.08ppb(0.08ng/g)6飼料0.08ppb(0.08ng/g)7血清/血漿0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反應(yīng)名稱百分比氯霉素堿0.4%甲基氯霉素<0.04%回收率No.樣品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，蝦，魚及肉類95%~120%4飼料95%~120%5血清/血漿90%~120%6尿液1**%~130%ProductName:MouseMonoclonalAnti-HumanLipoproteinAssociatedPhospholipaseA2(Lp-PLA2)CatalogNumber:70745Subclass:IgG1ProductForm:PBS,pH7.4with0.09%sodiumazideStorage:At2-8C.DoNotFreeze.Precipitationmayoccuruponfreezing.SuggestedUse:Forsolidphasecoating(capture)inthehumanLp-PLA2immunoassay.PairingInformation:ThisantibodypairswithMouseMonoclonalAnti-HumanLp-PLA2PartNo.70746orMouseMonoclonalAnti-HumanLp-PLA2PartNo.70747部分產(chǎn)品Zxin報(bào)價(jià)如下：貨號(hào)品名規(guī)格價(jià)格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我們公司Zda優(yōu)勢是強(qiáng)大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都有價(jià)格優(yōu)勢，5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們還是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......幾十家國外公司授權(quán)代理7：我們還從事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批發(fā)[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊

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• 國家ZD新產(chǎn)品

  國家ZD新產(chǎn)品[詳細(xì)]

  2003-04-07 00:00

  報(bào)價(jià)單

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• 歐司朗LED新產(chǎn)品

  歐司朗2014年新產(chǎn)品。[詳細(xì)]

  2018-10-30 10:00

  產(chǎn)品樣冊

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• socorex移液器新產(chǎn)品

  瑞士SOCOREX成立于1940年，是從事高精密液體操作產(chǎn)品的研發(fā)，生產(chǎn)及經(jīng)營的專業(yè)廠家。配備完善的技術(shù)支持，維修和校準(zhǔn)服務(wù)。產(chǎn)品包括：手動(dòng)，電動(dòng)移液器，多道移液器，瓶口配液器、移液管控制器、連續(xù)分液器、連續(xù)注射器，特殊用途移液器及各產(chǎn)品完備的附件系統(tǒng)?？梢詫?shí)現(xiàn)液體計(jì)量、定量給料，轉(zhuǎn)移傳輸、等量分配和液體注射等多項(xiàng)液體操作功能。瑞士SOCOREX公司通過對精密液體操作產(chǎn)品及優(yōu)質(zhì)服務(wù)的高度關(guān)注，通過了ISO9001和ISO13485質(zhì)量管理體系認(rèn)證。所有產(chǎn)品通過ISO8655，GLP，GMP和NCCLS認(rèn)證文件，同時(shí)所有產(chǎn)品出廠前進(jìn)行嚴(yán)格QC認(rèn)證，每個(gè)產(chǎn)品都有單獨(dú)出具的報(bào)告文件。SOCOREX每個(gè)精密儀器具備有其自身的序列號(hào)并通過了嚴(yán)格的品控測試，獨(dú)立的第三方認(rèn)證?！耙徊绞浇鉀Q方案”時(shí)SOCOREX服務(wù)ZX的格言。依據(jù)ISO17025標(biāo)準(zhǔn)，SOCOREX服務(wù)ZX提供了性的維修和校準(zhǔn)程序。[詳細(xì)]

  2024-10-10 02:50

  產(chǎn)品樣冊

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• FRITSCH加強(qiáng)型新產(chǎn)品簡介

  FRITSCH加強(qiáng)型新產(chǎn)品簡介[詳細(xì)]

  2016-05-02 00:00

  安裝說明

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• Wilkerson過濾器，Wilkerson新產(chǎn)品

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