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BioCheck新產(chǎn)品BC-1301 說明書

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2018-09-29 10:02 280閱讀次數(shù)

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世界ding級實驗材料供應(yīng)商BioCheck正式授權(quán)上海起發(fā)為其ZG代理,BioCheck在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來**的產(chǎn)品,**的服務(wù),簽約BioCheck就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲BioCheckZG代理,BioCheck上海代理,BioCheck北京代理,BioCheck廣東代理,BioCheck江蘇代理BioCheck湖北代理,BioCheck天津,BioCheck黑龍江代理,BioCheck內(nèi)蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江蘇代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由創(chuàng)始人Dr.JohnChen,Medix創(chuàng)立,是一家抓也提供腫瘤標(biāo)志物,心肌標(biāo)志物,激素類Z高性價比的抗體的公司。簡要原理利用競爭酶聯(lián)免疫方法,預(yù)先在微孔中包被羊抗兔抗體,實驗時先后加入氯霉素標(biāo)準(zhǔn)品或待測樣本,氯霉素酶標(biāo)抗原和兔抗氯霉素抗體。經(jīng)過室溫溫育,反應(yīng)液中的兔抗氯霉素抗體與微孔板上的羊抗兔抗體結(jié)合,待測樣品中的抗原與氯霉素酶標(biāo)抗原競爭微孔板上的兔抗氯霉素抗體。洗滌后,沒有與抗體結(jié)合的待測樣品中的抗原或酶標(biāo)抗原被洗去,再加入反應(yīng)底物,結(jié)合的酶標(biāo)抗原的酶將底物轉(zhuǎn)化為藍(lán)色產(chǎn)物,加入終止液后顏色由藍(lán)色變?yōu)辄S色。反應(yīng)完成后,樣品中氯霉素含量越多,反應(yīng)呈色就越淺;反之,樣品中氯霉素含量越少,則呈色越深。利用標(biāo)準(zhǔn)曲線可計算出樣品中氯霉素含量。技術(shù)參數(shù)檢測蜂蜜、蛋類、牛奶、奶粉、水產(chǎn)品、動物組織(肌肉、肝臟等)、飼料、血清、血漿及尿液樣本中存在的氯霉素,定量限可達(dá)0.025ppb。No.樣品檢測下限1蜂蜜0.02ppb(0.02ng/g)2蛋類0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5蝦,魚及肉類0.08ppb(0.08ng/g)6飼料0.08ppb(0.08ng/g)7血清/血漿0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反應(yīng)名稱百分比氯霉素堿0.4%甲基氯霉素<0.04%回收率No.樣品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋,蝦,魚及肉類95%~120%4飼料95%~120%5血清/血漿90%~120%6尿液1**%~130%PD-1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1301EnzymeImmunoassayfortheQuantitativeDeterminationofPD-1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammedcelldeathprotein1,(PD-1,CD279,PDCD1),isacellsurfacereceptorthatiscriticalfortheregulationofTcellinflammatoryactivityandmaintainsperipheraltoleranceintheimmunesystem(1).WithanextracellularIgVdomain,atransmembranedomain,andanintracellulartailwithtwophosphorylationsites(SHP-1andSHP-2),PD-1isabletointeractwithligandsPD-L1andPD-L2todown-regulatetheimmunesystemandsuppressTcellactivity(2).Uponantigenrecognition,anactivatedTcellexpressesPD-1onitssurfaceandproducesinterferons,whichinducesexpressionofPD-L1,therebypromotingapoptosisorcelldeath.(3)Howeverwhenhijackedbytumorcells,aberrantexpressionofPD-1ligandsinhibitsimmune-modulatoryTcellactivation,leadingtodiseaseprogression(4).ElevatedlevelsofPD-1inserumandplasmahavebeenassociatedwithrheumatoidarthritisandskinsclerosis(5,6).Also,PD-1ispredominantlyexpressedbytumorinfiltratingTcells(7).FurtherresearchimpliesthatusingmonoclonalantibodiestotargetthePD-1immunologiccheckpointhascontributedtobreakthroughprogressinunderstandingandtreatingcancerssuchasmelanomaandothervariousnon-smallcelllungcancer(4).PRINCIPLEOFTHEASSAYThePD-1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-1molecule.Onemousemonoclonalanti-PD-1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-150ng/mlPD-1Standard(0.5mL/vial)50ng/mLPD-1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute50ng/mLto10ng/mLwithStandard/Samplediluent.Preparetwo-foldserialdilutionsofthe10ng/mLStandardwithStandard/SampleDiluent:10ng/mL:0.12mLof50ng/mL+0.48mLofStandard/SampleDiluent5ng/mL:0.25mLof10ng/mL+0.25mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-1Absorbance(ng/ml)(450nm)00.0890.1560.1380.3130.1900.6250.2841.250.4812.50.85151.581102.776nm32.5(4502Absorbance1.510.500246810PD-1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.15ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-1(ng/mL)8.413.222.9S.D.0.61.00.9C.V.(%)7.0%7.2%3.8%.2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-1(ng/mL)9.113.525.3S.D.0.81.10.9C.V.(%)9.2%7.93.7RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-1levelsandassayedinduplicate.Themeanrecoverywas105.3%.PAIREXPECTEDOBSERVED%RECOVERYNUMBER[PD-1][PD-1](ng/mL)(ng/mL)188.8110.0%212.513.1104.8%32525.3101.2%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas99.6%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:210.1114.8%1:48.88.8N/A1:87.888.6%Mean=101.7%2.1:214.5110.6%1:413.113.1N/A1:811.688.5%Mean=99.6%3.1:226.5104.7%1:425.325.3N/A1:823.090.0%Mean=97.4%REFERENCESFife,B.T.,&Pauken,K.E.(2011).TheroleofthePD-1pathwayinautoimmunityandperipheraltolerance.AnnalsoftheNewYorkAcademyofSciences,1217(1),45-59.Riella,L.V.,Paterson,A.M.,Sharpe,A.H.,&Chandraker,A.(2012).RoleofthePD-1PathwayintheImmuneResponse.AmericanJournalofTransplantation:OfficialJournaloftheAmericanSocietyofTransplantationandtheAmericanSocietyofTransplantSurgeons,12(10),25752587.Zak,Krzysztof&Kitel,Radosaw&Przetocka,Sara&Golik,Przemysaw&Guzik,Katarzyna&Musielak,Bogdan&Dm領(lǐng),Alexander&Dubin,Grzegorz&Holak,Tad.(2015).StructureoftheComplexofHumanProgrammedDeath1,PD-1,andItsLigandPD-L1.Structure.23..10.1016/j.str.2015.09.010.ZoranGatalica,CarrieSnyder,ToddManey,AnatoleGhazalpour,DanielHolterman,NianqingXiao,PeggyOverberg,IngaRose,GargiD.Basu,SemirVranic,HenryT.Lynch,DanielD.VonHoffandOmidHamid.ProgrammedCellDeath1(PD-1)andItsLigand(PD-L1)inCommonCancersandTheirCorrelationwithMolecularCancerType.CancerEpidemiolBiomarkersPrev.December12014(23)(12)2965-2970.Greisen,S.,Rasmussen,T.,Stengaard-Pedersen,K.,Hetland,M.,Hrslev-Petersen,K.,Hvid,M.,&Deleuran,B.(2013).Increasedsolubleprogrammeddeath-1(sPD-1)isassociatedwithdiseaseactivityandradiographicprogressioninearlyrheumatoidarthritis.ScandinavianJournalofRheumatology,43(2),101-108.Yanaba,K.,Hayashi,M.,Yoshihara,Y.,&Nakagawa,H.(2016).Serumlevelsofsolubleprogrammeddeath-1andprogrammeddeathligand-1insystemicsclerosis:Associationwithextentofskinsclerosis.TheJournalofDermatology,43(8),954-957.Ahmadzadeh,M.,Johnson,L.A.,Heemskerk,B.,Wunderlich,J.R.,Dudley,M.E.,White,D.E.,&Rosenberg,S.A.(2009).TumorantigenspecificCD8TcellsinfiltratingthetumorexpresshighlevelsofPD-1andarefunctionallyimpaired.Blood,114(8),15371544.部分產(chǎn)品Zxin報價如下:貨號品名規(guī)格價格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我們公司Zda優(yōu)勢是強(qiáng)大的采購,1:基本什么都能進(jìn)口,血清,抗體,耗材,還有部分限制進(jìn)口的,2:貨品全,現(xiàn)經(jīng)營過700多個品牌,基本所有生物試劑耗材都可以進(jìn)口,特別是冷偏的產(chǎn)品那就更有優(yōu)勢,3:提供加急服務(wù),一般1-2周到貨,超過時限加急費全免4:價格公道,絕大部分價格有優(yōu)勢,當(dāng)然不能保證1**%產(chǎn)品都有價格優(yōu)勢,5:良好的信譽(yù),大部分客戶我們提供貨到付款服務(wù),客戶包括清華,北大交大復(fù)旦,中山等100多所大學(xué),ROCHE,阿斯利康,國藥,fisher等500多家公司6:我們還是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica;Ludger;neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO;quickzyme;RESEARCHDIETS,INC;sterlitech;sysy;TriLinkBioTechnologies,Inc;BIOCHECKINC-biochem;zyagen;......幾十家國外公司授權(quán)代理7:我們還從事invitrogen,qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批發(fā)

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