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• Wilkerson過(guò)濾器，Wilkerson新產(chǎn)品

  Wilkerson過(guò)濾器，Wilkerson新產(chǎn)品Wilkerson威爾克森過(guò)濾器由簡(jiǎn)體、不銹鋼濾網(wǎng)、排污部分、傳動(dòng)裝置及電氣控制部分組成。過(guò)濾器工作時(shí)，待過(guò)濾的水由水口過(guò)濾器流Wilkerson威爾克森過(guò)濾器經(jīng)濾網(wǎng)，通過(guò)出口進(jìn)入用戶所須的管道進(jìn)行工藝循環(huán)，水中的顆粒雜質(zhì)被截留在濾網(wǎng)內(nèi)部。如此不斷的循環(huán)，被截留下來(lái)的顆粒越來(lái)越多，過(guò)濾速度越來(lái)越慢，而進(jìn)口的污水仍源源不斷地進(jìn)入，濾孔會(huì)越來(lái)越小，由此在進(jìn)、出口之間產(chǎn)生壓力差，當(dāng)大度差達(dá)到設(shè)定值時(shí)，差壓變送器將電信號(hào)傳送到控制器，控制系統(tǒng)啟動(dòng)驅(qū)動(dòng)馬達(dá)通過(guò)傳動(dòng)組件帶動(dòng)軸轉(zhuǎn)動(dòng)，同時(shí)排污口打開，由排污口排出，當(dāng)濾網(wǎng)清洗完畢后，壓差降到Z小值，系統(tǒng)返回到初始過(guò)濾狀，系統(tǒng)正常運(yùn)行。Wilkerson威爾克森過(guò)濾器由殼體、多元濾芯、反沖洗機(jī)構(gòu)、和差壓控制器等部分組成。殼體內(nèi)的橫隔板將其內(nèi)腔分為上、下兩腔，上腔內(nèi)配有多個(gè)過(guò)濾芯，這樣充分利用了過(guò)濾空間，顯著縮小了過(guò)濾器的體積，下腔內(nèi)安裝有反沖洗吸盤。工作時(shí)，濁液經(jīng)入口進(jìn)入過(guò)濾器下腔，又經(jīng)隔板孔進(jìn)入濾芯的內(nèi)腔。大于過(guò)濾芯縫隙的雜質(zhì)被截留，凈液穿過(guò)縫隙到達(dá)上腔，Z后從出口送出。新產(chǎn)品查看新的Wilkerson產(chǎn)品！查看Wilkerson的R90系列。90系列允許在不使用管道連接器的情況下將單元連接在一起。這樣可以節(jié)省空間，提供恒定的安裝ZX，并保持現(xiàn)代的美觀外觀。新目錄和產(chǎn)品列表！R90系列產(chǎn)品目錄下載目錄607：R90系列FLR產(chǎn)品其他新產(chǎn)品文獻(xiàn)：公告9EM-B3：R120調(diào)節(jié)器公告9EM-B1：D級(jí)工業(yè)呼吸空氣的空氣準(zhǔn)備目錄604：氣動(dòng)產(chǎn)品-精密調(diào)節(jié)器FRL-SIF-619：精密調(diào)節(jié)器清潔干燥空氣系統(tǒng)的更多信息（PDF）目錄：603：烘干機(jī)產(chǎn)品：液體分離器，排水閥和制冷干燥機(jī)FRL-SIF-618：壓縮空氣干燥指南FRL-TEC-600：聚碳酸酯碗化學(xué)兼容性Wilkerson過(guò)濾器，Wilkerson新產(chǎn)品New!90SeriesFRLProductsSafetyLockOutsCombinedSoftStartandRemoteOperatedDumpValveHighPrecisionRegulatorsWSA/WSOSeriesLiquidSeparatorWDV3AutomaticElectricalDrainValveWDV2SeriesZeroLossDrainWDRDSeriesRefrigerationDryerModularBallValveWilkerson過(guò)濾器，Wilkerson新產(chǎn)品[詳細(xì)]

  2018-09-21 10:00

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• 美國(guó)WILKERSON過(guò)濾器,WILKERSON減壓閥

  美國(guó)WILKERSON過(guò)濾器,WILKERSON減壓閥產(chǎn)品有過(guò)濾器，油霧器，調(diào)壓器等，有F系列，A系列，B系列，D系列，E系列，F(xiàn)系列，G系列，M系列，R系列，L系列，N系列，P系列，X系列，V系列，CB系列等wilkerson（威爾克森）生產(chǎn)和銷售全系列，壓縮空氣處理元件及控制產(chǎn)品。其中包括過(guò)濾器，穩(wěn)壓器和潤(rùn)滑劑，空氣干燥器，分離器，排水渠和其他組件在空氣制備。Wilkerson所提供的優(yōu)質(zhì)產(chǎn)品滿足各種各樣的工業(yè)，加工行業(yè)，致力于消費(fèi)者和健康保健應(yīng)用。[詳細(xì)]

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• wilkerson顆粒過(guò)濾器

  wilkerson顆粒過(guò)濾器威爾克森wilkerson公司主要制造的產(chǎn)品有美國(guó)wilkerson顆粒過(guò)濾器、美國(guó)wilkerson凝聚式過(guò)濾器、美國(guó)wilkerson后過(guò)濾器、代理美國(guó)wilkerson調(diào)節(jié)器、美國(guó)wilkerson高精密穩(wěn)壓器、美國(guó)wilkerson潤(rùn)滑器、美國(guó)wilkerson過(guò)濾器、美國(guó)wilkerson調(diào)節(jié)閥、美國(guó)wilkerson組合電器、美國(guó)wilkerson干燥器、美國(guó)wilkerson不銹鋼鋼制FRLs、美國(guó)wilkerson配件及維修工具包等等。wilkerson是高質(zhì)量，高性能，高價(jià)值的代名詞，專業(yè)生產(chǎn)和銷售全系列壓縮空氣處理元件及控制產(chǎn)品。其中包括過(guò)濾器，穩(wěn)壓器和潤(rùn)滑劑，空氣干燥器，分離器，排水渠和其他組件。R12系列如：R12-02-F0G0R12-03-R0G0R18系列如：R18-02-R0G0R18-03-WOG0R18-04-W000R28系列如：R28-03-D0G0R28-04-R000R28-06-D000R39系列如：R39-06-F000R39-08-F000*刻度盤式調(diào)壓閥R11系列如：R11-C2-000(有大量現(xiàn)貨）R21系列如：R21-00CP/M1R21-04B/M1R31系列如：R31-06-R00R31-C8-RV0R41系列如：R41-CC-R00R41-CB-000R41-CB-R00*精密型調(diào)壓閥P12系列如：P12-02-F000P12-02-A000P15系列如：P15-02-000P15-03-NV0P15-C4-000P16系列如：P16-02-H00P16-03-000P16-C4-V00*高精度調(diào)壓閥P17系列如：P17-02-B00P17-02-F00P17-02-FH0*高流量精密調(diào)壓閥P19系列如：P19-02-A00P19-02-H00P19-03-D00*普通型調(diào)壓閥R09系列如：R09-01-CB00R09-02-RB00R19系列如：R19-02-GA00R19-03-SA00R19-04-WA00wilkerson顆粒過(guò)濾器ParticulateFiltersCoalescingFiltersAfterfiltersRegulatorsHighPrecisionRegulatorsLubricatorsFilter/RegulatorsCombinationUnitsNew!IndustrialBreathingAirStainlessSteelFRLsAccessories&RepairKitsDryersAdditionalProductswilkerson顆粒過(guò)濾器[詳細(xì)]

  2018-09-21 10:00

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• WILKERSON過(guò)濾器技術(shù)資料

  WILKERSON過(guò)濾器選型參數(shù)及技術(shù)資料[詳細(xì)]

  2018-11-01 10:00

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• Wilkerson過(guò)濾器，90系列過(guò)濾器

  Wilkerson過(guò)濾器，90系列過(guò)濾器Wilkerson顆粒過(guò)濾器聚結(jié)過(guò)濾器調(diào)節(jié)器先導(dǎo)式調(diào)壓器過(guò)濾器/調(diào)節(jié)器潤(rùn)滑比例壓力調(diào)節(jié)器組合軟啟動(dòng)/傾卸閥和遠(yuǎn)程操作的傾卸閥軟啟動(dòng)閥模塊化球閥模塊化歧管和可選端口組件套件消除管道連接器，節(jié)省空間90系列允許在不使用管道連接器的情況下將單元連接在一起。這樣可以節(jié)省空間，提供恒定的安裝ZX，并保持現(xiàn)代的美觀外觀。90系列FRL新！90系列過(guò)濾器專門設(shè)計(jì)用于有效地過(guò)濾掉壓縮空氣管路中的銹蝕，污垢和其他雜質(zhì)。操作是全自動(dòng)的，并具有Z小的壓降。90系列調(diào)節(jié)器旨在為Z苛刻的高流量工業(yè)應(yīng)用提供快速響應(yīng)和極ng確的壓力調(diào)節(jié)。滾動(dòng)隔膜設(shè)計(jì)用于長(zhǎng)時(shí)間無(wú)故障運(yùn)行，不會(huì)在高循環(huán)或苛刻應(yīng)用下破裂或撕裂。90系列霧化潤(rùn)滑器設(shè)計(jì)用于為許多通用應(yīng)用提供潤(rùn)滑。Wilkerson過(guò)濾器，90系列過(guò)濾器Wilkerson過(guò)濾器，90系列過(guò)濾器[詳細(xì)]

  2018-09-21 10:00

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• Wilkerson過(guò)濾器L08-02-KK00資料概述

  Wilkerson過(guò)濾器L08-02-KK00資料概述工作原理過(guò)濾器工作時(shí)，待過(guò)濾的水由水口時(shí)入，流經(jīng)濾網(wǎng)，過(guò)濾器(26張)通過(guò)出口進(jìn)入用戶所須的管道進(jìn)行工藝循環(huán)，水中的顆粒雜技被截留在濾網(wǎng)內(nèi)部。如此不斷的循環(huán)，被截留下來(lái)的顆粒越來(lái)越多，過(guò)濾速度越來(lái)越慢，而進(jìn)口的污水仍源源不斷地進(jìn)入，濾孔會(huì)越來(lái)越小，由此在進(jìn)、出口之間產(chǎn)生壓力差，當(dāng)大度差達(dá)到設(shè)定值時(shí)，差壓變送器將電信號(hào)傳送到控制器，控制系統(tǒng)啟動(dòng)驅(qū)動(dòng)馬達(dá)通過(guò)傳動(dòng)組件帶動(dòng)軸轉(zhuǎn)動(dòng)，同時(shí)排污口打開，由排污口排出，當(dāng)濾網(wǎng)清洗完畢后，壓差降到Z小值，系統(tǒng)返回到初始過(guò)濾狀，系統(tǒng)正常運(yùn)行。過(guò)濾器由殼體、多元濾芯、反沖洗機(jī)構(gòu)、和差壓控制器等部分組成。殼體內(nèi)的橫隔板將其內(nèi)腔分為上、下兩腔，上腔內(nèi)配有多個(gè)過(guò)濾芯，這樣充分了過(guò)濾空間，顯著縮小了過(guò)濾器的體積，下腔內(nèi)安裝有反沖洗吸盤。工作時(shí)，濁液經(jīng)入口進(jìn)入過(guò)濾器下腔，又經(jīng)隔板孔進(jìn)入濾芯的內(nèi)腔。大于過(guò)濾芯縫隙的雜質(zhì)被截留，凈液穿過(guò)縫隙到達(dá)上腔，Z后從出口送出。過(guò)濾器采用高強(qiáng)度的楔形濾網(wǎng)，通過(guò)壓差控制、定時(shí)控制自動(dòng)清洗濾芯。當(dāng)過(guò)濾器內(nèi)雜質(zhì)積聚在濾芯表面引起進(jìn)出口壓差增大到設(shè)定值，或定時(shí)器達(dá)到預(yù)置時(shí)間時(shí)，電動(dòng)控制箱發(fā)出信號(hào)，驅(qū)動(dòng)反沖洗機(jī)構(gòu)。當(dāng)反沖洗吸盤與濾芯進(jìn)口正對(duì)時(shí)，排污閥打開，此時(shí)系統(tǒng)泄壓排水，吸盤與濾芯內(nèi)側(cè)出現(xiàn)一個(gè)相對(duì)壓力低于濾芯外側(cè)水壓的負(fù)壓區(qū)，迫使部分凈循環(huán)水從濾芯外側(cè)流入濾芯內(nèi)側(cè)，吸附在濾芯內(nèi)內(nèi)壁上的雜質(zhì)微粒隨水流進(jìn)盤內(nèi)并從排污閥排出。特殊設(shè)計(jì)的濾網(wǎng)使得濾芯內(nèi)部產(chǎn)生噴射效果，任何雜質(zhì)都將被從光滑的內(nèi)壁上沖走。當(dāng)過(guò)濾器進(jìn)出口壓差恢復(fù)正?；蚨〞r(shí)器設(shè)定時(shí)間結(jié)束，整個(gè)過(guò)程中，物料不斷流，反洗耗水量少，實(shí)現(xiàn)了連續(xù)化，自動(dòng)化生產(chǎn)。過(guò)濾器廣泛用于冶金、化工、石油、造紙、醫(yī)藥、食品、采礦、電力、城市給水領(lǐng)域。諸如工業(yè)廢水，循環(huán)水的過(guò)濾，乳化液的再生，廢油過(guò)濾處理，冶金行業(yè)的連鑄水系統(tǒng)、高爐水系統(tǒng)，熱軋用高壓水除鱗系統(tǒng)。是一種先進(jìn)、GX且易操作的全自動(dòng)過(guò)濾裝置。過(guò)濾器待處理的水由入水口進(jìn)入機(jī)體，水中的雜質(zhì)沉積在不銹鋼濾網(wǎng)上，由此產(chǎn)生壓差。通過(guò)壓差開關(guān)監(jiān)測(cè)進(jìn)出水口壓差變化，當(dāng)壓差達(dá)到設(shè)定值時(shí)，電控器給水力控制閥，驅(qū)動(dòng)電機(jī)信號(hào)。設(shè)備安裝后，由技術(shù)人員進(jìn)行調(diào)試，設(shè)定過(guò)濾時(shí)間和清洗轉(zhuǎn)換時(shí)間，待處理的水由入水口進(jìn)入機(jī)體，過(guò)濾器開始正常工作，當(dāng)達(dá)到預(yù)設(shè)清洗時(shí)間時(shí)，電控器給水力控制閥、驅(qū)動(dòng)電機(jī)信號(hào)，引發(fā)下列動(dòng)作：電動(dòng)機(jī)帶動(dòng)刷子旋轉(zhuǎn)，對(duì)濾芯進(jìn)行清洗，同時(shí)控制閥打開進(jìn)行排污，整個(gè)清洗過(guò)程只需持續(xù)數(shù)十秒鐘，當(dāng)清洗結(jié)束時(shí)，關(guān)閉控制閥，電機(jī)停止轉(zhuǎn)動(dòng)，系統(tǒng)恢復(fù)至其初始狀態(tài)，開始進(jìn)入下一個(gè)過(guò)濾工序。過(guò)濾器的殼體內(nèi)部主要由粗濾網(wǎng)、細(xì)濾網(wǎng)、吸污管，不銹鋼刷或不銹鋼吸嘴、密封圈、防腐涂層、轉(zhuǎn)動(dòng)軸等組成。過(guò)濾器(10張)用過(guò)濾介質(zhì)把容器分隔為上、下腔即構(gòu)成簡(jiǎn)單的過(guò)濾器。懸浮液加入上腔，在壓力作用下通過(guò)過(guò)濾介質(zhì)進(jìn)入下腔成為濾液，固體顆粒被截留在過(guò)濾介質(zhì)表面形成濾渣(或稱濾餅)。過(guò)濾過(guò)程中過(guò)濾介質(zhì)表面積存的濾渣層逐漸加厚，液體通過(guò)濾渣層的阻力隨之，過(guò)濾速度減小。當(dāng)濾室充滿濾渣或過(guò)濾速度太小時(shí)，停止過(guò)濾，清除濾渣，使過(guò)濾介質(zhì)再生，以完成一次過(guò)濾循環(huán)。液體通過(guò)濾渣層和過(guò)濾介質(zhì)必須克服阻力，因此在過(guò)濾介質(zhì)的兩側(cè)必須有壓力差，這是實(shí)現(xiàn)過(guò)濾的推動(dòng)力。增大壓力差可以加速過(guò)濾，但受壓后變形的顆粒在大壓力差時(shí)易堵塞過(guò)濾介質(zhì)孔隙，過(guò)濾反而減慢。懸浮液過(guò)濾有濾渣層過(guò)濾、深層過(guò)濾和篩濾3種方式。①濾渣層過(guò)濾：過(guò)濾初期過(guò)濾介質(zhì)只能截留大的固體顆粒，小顆粒隨濾液穿過(guò)過(guò)濾介質(zhì)。在形成初始濾渣層后，濾渣層對(duì)過(guò)濾起主要作用，這時(shí)大、小顆粒均被截留，例如板框壓濾機(jī)的過(guò)濾。②深層過(guò)濾：過(guò)濾介質(zhì)較厚，懸浮液中含固體顆粒較少，且顆粒小于過(guò)濾介質(zhì)的孔道。過(guò)濾時(shí)，顆粒進(jìn)入后被吸附在孔道內(nèi)，例如多孔塑料管過(guò)濾器、砂濾器的過(guò)濾。③篩濾：過(guò)濾截留的固體顆粒都大于過(guò)濾介質(zhì)的孔隙，過(guò)濾介質(zhì)內(nèi)部不吸附固體顆粒，例如轉(zhuǎn)筒式過(guò)濾篩濾去污水中的粗粒雜質(zhì)。在實(shí)際的過(guò)濾過(guò)程中，三種方式常常是同時(shí)或相繼出現(xiàn)。美國(guó)Wilkerson（威爾克森）生產(chǎn)和銷售全系列壓縮空氣處理元件及控制產(chǎn)品。其中包括過(guò)濾器，穩(wěn)壓器，潤(rùn)滑劑，空氣干燥器，分離器，排水渠和其他組件的空氣制備。Wilkerson所提供的優(yōu)質(zhì)產(chǎn)品滿足各種各樣的工業(yè)，加工行業(yè)，致力于消費(fèi)者和健康保健的應(yīng)用。過(guò)濾器廠家-東莞艾科,專業(yè)供應(yīng)過(guò)濾器.過(guò)濾器價(jià)格低廉,品質(zhì)保證.艾科過(guò)濾器安全可靠.咨詢熱線威爾克森微粒過(guò)濾器展示相關(guān)型號(hào)介紹F01系列如：F01-C2-000F03系列如：F03-02-000F16系列如：F16-02-D00F16-03-M00F16-04-FG0F26系列如：F26-02-F00F26-03-DF0F26-04-000F30系列如：F30-06-000F30-C8-DFMF34系列如：F34-04-000F34-06-M00F34-C8-M00F35系列如：F35-0A-000F35-0B-F00F35-0C-H00F36系列如：F36-0C-F00F36-CC-000F42系列如：F42-0A-000F42-0B-FM0F42-0C-FM0F43系列如：F43-0E-000F43-CE-H00F08系列如：F08-01-SK00F08-02-SR00F12系列如：F12-02-SK00F12-03-SR00F18系列如:F18-02-SK00F18-03-SL00F18-04-SGL0F28系列如：F28-03-SK00F28-04-SG00F28-06-SKL0F39系列如：F39-06-SL00F39-08-SH00調(diào)壓過(guò)濾器B08系列如：B08-01-CK00B08-02-CKC0B18系列如：B18-02-CGCGB18-03-FM00B18-04-JKG0B28系列如：B28-03-FL00B28-04-FHG0B28-06-FKP0B39系列如：B39-06-FL00B39-C8-FH00美國(guó)wilkerson威爾克森過(guò)濾器F18-02-SG00M18-02-CG00A18-02-BKM0F18-02-SG00M18-02-CG00A18-02-BKM0F18-02-SG00M18-02-CG00A18-02-BKM0F28-04-SG00M28-04-CG00A28-04-BKM0F28-04-SG00M28-04-CG00A28-04-BKM0F28-04-SG00M28-04-CG00A28-04-BKM0[詳細(xì)]

  2018-10-06 10:02

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• wilkerson果粒過(guò)濾器中文資料

  wilkerson果粒過(guò)濾器中文資料wilkerson公司主要制造的產(chǎn)品有美國(guó)wilkerson顆粒過(guò)濾器、美國(guó)wilkerson凝聚式過(guò)濾器、美國(guó)wilkerson后過(guò)濾器、美國(guó)wilkerson調(diào)節(jié)器、美國(guó)wilkerson高精密穩(wěn)壓器、美國(guó)wilkerson潤(rùn)滑器、美國(guó)wilkerson過(guò)濾器/調(diào)節(jié)閥、美國(guó)wilkerson組合電器、美國(guó)wilkerson干燥器、美國(guó)wilkerson不銹鋼鋼制FRLs、美國(guó)wilkerson配件及維修工具包等等。我公司專做歐美品牌，所有歐美品牌直接境外歐美國(guó)家采購(gòu)，本公司可以直接報(bào)關(guān)，貨期可以縮短一半，價(jià)格更有優(yōu)勢(shì)可以為您爭(zhēng)取更大的利潤(rùn)空間。如有需要請(qǐng)直接電話聯(lián)系或直接來(lái)本公司視察：以下品牌是我公司優(yōu)勢(shì)品牌：一：德國(guó)HAWE德國(guó)REXROTH德國(guó)FESTO德國(guó)BURKERT德國(guó)E+H德國(guó)西門子德國(guó)STAUFF西德福德國(guó)SCHMERSAL德國(guó)NEGELE耐格德國(guó)EMG（只做SV伺服閥系列）德國(guó)STEIMEL德國(guó)赫斯曼德國(guó)MURR德國(guó)HYADC德國(guó)P+F德國(guó)TURCK圖爾克德國(guó)SICK施克德國(guó)IFM博恩斯坦德國(guó)GSR德國(guó)PILZ德國(guó)MAHLE德國(guó)巴魯夫德國(guó)冬斯德國(guó)勞易測(cè)德國(guó)Linde林德德國(guó)COAX二:意大利ATOS意大利UNIVER意大利杰弗倫英國(guó)海隆諾冠意大利萬(wàn)福樂(lè)法國(guó)高諾斯意大利康茂盛三:美國(guó)ASCOPARKER派克美國(guó)克力帕VICKERS威格士美國(guó)MOOG美國(guó)MAC美國(guó)紐曼帝克美國(guó)德威爾公司還有代理部分日本品牌（日本品牌直接辦事處采購(gòu)）如下：CKD喜開理，黑田精工，SUNX神視，TOYOOKI豐興，NACHI不二越，DAIKIN大金，KOGANEI小金井，TACO,NOK,東京美?！疚夜究梢宰约哼M(jìn)口報(bào)關(guān)】wilkerson果粒過(guò)濾器中文資料WILKERSON是專業(yè)生產(chǎn)氣源處理產(chǎn)品的制造商,高品質(zhì)及全系列的規(guī)格適用于各種不同的工業(yè)應(yīng)用,主要的產(chǎn)品規(guī)格型號(hào)如下:F00-01-000F00-01-B00F00-01-M00F00-02-000F00-02-B00F00-02-M00F00-01-D00F00-01-BD0F00-01-DM0F00-02-D00F00-02-BD0F00-02-DM0F08-01-SK00F08-01-SL00F08-02-SK00F08-02-SL00F08-01-SR00F08-01-SS00F08-02-SR00F08-02-SS00F12-02-SK00F12-02-SL00F12-03-SK00F12-03-SL00F12-02-SR00F12-02-SS00F12-03-SR00F12-03-SS00F16-02-000F16-02-M00F16-02-G00F16-02-D00F16-03-000F16-03-M00F16-03-G00F16-03-D00F16-04-000F16-04-M00F16-04-G00F16-04-D00F16-02-F00F16-02-FM0F16-02-FG0F16-02-DF0F16-03-F00F16-03-FM0F16-03-FG0F16-03-DF0F16-04-F00F16-04-FM0F16-04-FG0F16-04-DF0F18-02-SK00F18-02-SL00F18-02-SKL0F18-03-SK00F18-03-SL00F18-03-SKL0F18-04-SK00F18-04-SL00F18-04-SKL0F18-02-SG00F18-02-SH00F18-02-SGL0F18-03-SG00F18-03-SH00F18-03-SGL0F18-04-SG00F18-04-SH00F18-04-SGL0F26-02-000F26-02-M00F26-02-G00F26-02-D00F26-03-000F26-03-M00F26-03-G00F26-03-D00F26-04-000F26-04-M00F26-04-G00F26-04-D00F26-02-F00F26-02-FM0F26-02-FG0F26-02-DF0F26-03-F00F26-03-FM0F26-03-FG0F26-03-DF0F26-04-F00F26-04-FM0F26-04-FG0F26-04-DF0F28-03-SK00F28-03-SL00F28-03-SKL0F28-04-SK00F28-04-SL00F28-04-SKL0F28-06-SK00F28-06-SL00F28-06-SKL0F28-03-SG00F28-03-SH00F28-03-SGL0F28-04-SG00F28-04-SH00F28-04-SGL0F28-06-SG00F28-06-SH00F28-06-SGL0F30-06-000F30-06-M00F30-06-G00F30-06-D00F30-08-000F30-08-M00F30-08-G00F30-08-D00F30-06-F00F30-06-FM0F30-06-FG0F30-06-DF0F30-08-F00F30-08-FM0F30-08-FG0F30-08-DF0F34-04-000F34-04-M00F34-06-000F34-06-M00F34-08-000F34-08-M00F35-0A-000F35-0C-000F35-0A-F00F35-0B-F00F35-0C-F00F35-0A-000P3NKA00BSAP3NKA00BSMP3NKA00ESGP3NKA00ESEP3NKA00ESAwilkerson果粒過(guò)濾器中文資料[詳細(xì)]

  2018-10-06 10:01

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• WILKERSON過(guò)濾器選型參數(shù)及技術(shù)資料

  WILKERSON過(guò)濾器選型參數(shù)及技術(shù)資料[詳細(xì)]

  2018-11-01 10:00

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• wilkerson比例壓力調(diào)節(jié)器

  wilkerson比例壓力調(diào)節(jié)器wilkerson比例壓力調(diào)節(jié)器460/5000ER90系列比例壓力調(diào)節(jié)器ER90系列-比例調(diào)節(jié)器查看公告查看90系列目錄下載CAD文件（zip）特征該比例排放器提供：積分3/4“或1”端口（BSPP和NPT）極ng確的輸出壓力響應(yīng)時(shí)間非?？靾?jiān)固而輕便的設(shè)計(jì)產(chǎn)品規(guī)格端口大小3/4"1"控制信號(hào)0-10V輸出信號(hào)0-10V出口壓力0-10bar（0至145psig）重量2.7磅（1.2kg）wilkerson比例壓力調(diào)節(jié)器wilkerson威爾克森90系列允許單元連接在一起不使用管道連接器。這節(jié)省了空間，提供恒定的安裝ZX，并維護(hù)a現(xiàn)代美學(xué)外觀美觀。90系列過(guò)濾器是專為有效設(shè)計(jì)的過(guò)濾掉銹，污垢，水分等雜質(zhì)壓縮空氣管路。操作全自動(dòng)Z小壓降。聚合過(guò)濾器和用于高純度空氣的吸附器過(guò)濾器也包括在內(nèi)90系列。90系列調(diào)節(jié)器設(shè)計(jì)提供快速響應(yīng)和極ng確的壓力調(diào)節(jié)Z多要求高流量工業(yè)應(yīng)用。滾動(dòng)隔膜設(shè)計(jì)為無(wú)故障操作不會(huì)在高周期下破裂或撕裂苛刻的應(yīng)用。90系列霧化潤(rùn)滑器旨在為許多通用產(chǎn)品提供潤(rùn)滑目的應(yīng)用wilkerson比例壓力調(diào)節(jié)器[詳細(xì)]

  2018-09-21 10:00

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• WILKERSON調(diào)壓閥型號(hào)下載

  WILKERSON調(diào)壓閥型號(hào)下載美國(guó)WILKERSON(威克森)空氣調(diào)壓閥產(chǎn)品型號(hào):R11系列R21系列R31系列R41系列產(chǎn)品種類:手動(dòng)刻度盤調(diào)節(jié)型先導(dǎo)遠(yuǎn)程控制調(diào)節(jié)型低壓力高精度調(diào)節(jié)型大流量調(diào)節(jié)型產(chǎn)品特點(diǎn):遠(yuǎn)程先導(dǎo)控制調(diào)節(jié)壓力,解決大流量調(diào)節(jié)精度問(wèn)題.duyi無(wú)二的刻度盤式調(diào)壓手柄,更直觀,更高精度!歡迎咨詢選型安裝/配件更換等問(wèn)題,資料備索!美國(guó)威爾克森的話我們直接美國(guó)原廠家訂貨回來(lái)的，在價(jià)格方面有一定有優(yōu)勢(shì)的。我們公司保證所有的價(jià)格都是一手貨源的，全新原裝貨源。真誠(chéng)的希望您的來(lái)電咨詢！WILKERSON威爾克森調(diào)壓閥的示例型號(hào)如下：R18-02-C000WILKERSONREGULATOR1/4"R18-02-C0G0WILKERSONREGULATOR1/4"R18-02-D000WILKERSONREGULATOR1/4"R18-02-D0CGWILKERSONREGULATOR1/4"R18-02-D0G0WILKERSONREGULATOR1/4"R18-02-D0GPWILKERSONREGULATOR1/4"R18-02-D0P0WILKERSONREGULATOR1/4"R18-02-F000WILKERSONREGULATOR1/4"R18-02-F0CGWILKERSONREGULATOR1/4"R18-02-F0G0WILKERSONREGULATOR1/4"R18-02-F0GPWILKERSONREGULATOR1/4"R18-02-F0GRWILKERSONREGULATOR1/4"R18-02-F0P0WILKERSONREGULATOR1/4"R18-02-F0R0WILKERSONREGULATOR1/4"R18-02-G000WILKERSONREGULATOR1/4"R18-02-G0G0WILKERSONREGULATOR1/4"R18-02-G0P0WILKERSONREGULATOR1/4"R18-02-J0CGWILKERSONREGULATOR1/4"R18-02-J0G0WILKERSONREGULATOR1/4"R18-02-L000WILKERSONREGULATOR1/4"R18-02-L0G0WILKERSONREGULATOR1/4"R18-02-P000WILKERSONREGULATOR1/4"R18-02-R000WILKERSONREGULATOR1/4"R18-02-R0G0WILKERSONREGULATOR1/4"R18-02-R0GPWILKERSONREGULATOR1/4"R18-02-S000WILKERSONREGULATOR1/4"R18-02-S0G0WILKERSONREGULATOR1/4"R18-02-W000WILKERSONREGULATOR1/4"R18-03-C000WILKERSONREGULATOR3/8"R18-03-C0G0WILKERSONREGULATOR3/8"R18-03-D000WILKERSONREGULATOR3/8"R18-03-D0CGWILKERSONREGULATOR3/8"R18-03-D0G0WILKERSONREGULATOR3/8"R18-03-D0P0WILKERSONREGULATOR3/8"R18-03-F000WILKERSONREGULATOR3/8"R18-03-F0C0WILKERSONREGULATOR3/8"R18-03-F0CRWILKERSONREGULATOR3/8"R18-03-F0G0WILKERSONREGULATOR3/8"R18-03-F0GRWILKERSONREGULATOR3/8"R18-03-F0P0WILKERSONREGULATOR3/8"R18-03-F0R0WILKERSONREGULATOR3/8"R18-03-G000WILKERSONREGULATOR3/8"R18-03-G0G0WILKERSONREGULATOR3/8"R18-03-G0GPWILKERSONREGULATOR3/8"R18-03-G0P0WILKERSONREGULATOR3/8"R18-03-J0G0WILKERSONREGULATOR3/8"R18-03-K0G0WILKERSONREGULATOR3/8"R18-03-L0G0WILKERSONREGULATOR3/8"R18-03-R000WILKERSONREGULATOR3/8"R18-03-R0G0WILKERSONREGULATOR3/8"R18-03-S000WILKERSONREGULATOR3/8"R18-03-S0G0WILKERSONREGULATOR3/8"R18-03-W0G0WILKERSON3/8"REGULATORR18-03-Y000WILKERSONREGULATOR3/8"R18-04-C000WILKERSONREGULATOR1/2"R18-04-C0CGWILKERSONREGULATOR1/2"R18-04-C0G0WILKERSONREGULATOR1/2"R18-04-D000WILKERSONREGULATOR1/2"R18-04-D0G0WILKERSONREGULATOR1/2"R18-04-D0GRWILKERSONREGULATOR1/2"R18-04-F000WILKERSONREGULATOR1/2"R18-04-F0C0WILKERSONREGULATOR1/2"R18-04-F0CGWILKERSONREGULATOR1/2"R18-04-F0G0WILKERSONREGULATOR1/2"R18-04-F0GRWILKERSONREGULATOR1/2"R18-04-F0P0WILKERSONREGULATOR1/2"R18-04-F0R0WILKERSONREGULATOR1/2"R18-04-G000WILKERSONREGULATOR1/2"WILKERSON調(diào)壓閥型號(hào)下載[詳細(xì)]

  2018-10-06 10:02

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• 美國(guó)WILKERSON威克森顆粒過(guò)濾器選型資料下載

  美國(guó)WILKERSON威克森顆粒過(guò)濾器F01系列港口規(guī)模1/4;流到17SCFM特性這個(gè)小、鋁管路過(guò)濾器設(shè)計(jì)提供保護(hù)便攜式氣動(dòng)手工具。它的重量只有2盎司信口開河的濾波元件額定5微米。端口可能作為進(jìn)氣口。流是17SCFM(8dm3/s)90PSIG(6.2條)的入口壓力與5PSIG(0.3條)壓降。規(guī)范流量*17.0SCFM(8dm3/秒)Zda的供應(yīng)壓力200PSIG(13.8條)工作溫度32°-150°F(0°到65.5°C)港口規(guī)模BSPT-Rc1/4標(biāo)準(zhǔn)的過(guò)濾5微米重量。13磅(.06kg)*進(jìn)口壓力150PSIG(10.3條)。壓降5PSID(0.3條)?！癋”系列過(guò)濾器,“A”型5微米的元素威爾克森:所有的“A”型5微米元素達(dá)到或超過(guò)ISO3班的Zda粒徑和固體污染物的濃度。美國(guó)WILKERSON威克森顆粒過(guò)濾器型號(hào)我們公司專業(yè)供應(yīng)進(jìn)口電磁閥、氣缸、泵、傳感器、繼電器、開關(guān)、離合器、過(guò)濾器、濾芯、流量計(jì)、液位計(jì)、編碼器、伺服閥等產(chǎn)品。詳請(qǐng)致電[詳細(xì)]

  2018-09-04 10:00

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• wilkerson 90系列調(diào)節(jié)器技術(shù)理

  wilkerson90系列調(diào)節(jié)器技術(shù)理90系列組合軟啟動(dòng)/傾卸閥，提供安全地引入對(duì)機(jī)器或系統(tǒng)的壓力。柔軟的啟動(dòng)/傾卸閥設(shè)置時(shí)，允許壓力逐漸增加在完全開放之前建立到設(shè)定點(diǎn)，以提供完整的流量線壓力?？刂茐毫Φ囊肟赡苁侵匾陌踩禂?shù)，防止空氣壓力下工具損壞在機(jī)器或系統(tǒng)啟動(dòng)時(shí)引入。wilkerson威爾克森產(chǎn)品規(guī)格工作壓力范圍P1min1bar（14.5psig）進(jìn)口壓力1P1Zda16bar（232psig）工作壓力范圍P2min0.2bar（2.9psig）出口壓力P2Zda10bar（145psig）工作溫度0°C至50°C（32°F至122°F）Zda流量Qnl/min20000m3/h1200SCFM706滯后P2max<1％重復(fù)性P2Zda<0.5％靈敏度P2Zda<0.5％線性P2Zda<1％標(biāo)稱電壓UnVDC24V=±10％殘余紋波10％功耗IBmax0.1設(shè)定值輸入U(xiǎn)wV0-10ImA0-20mA4-20輸入電阻RE243KΩ實(shí)際閥輸出UX0-10V輸出電流IAmax10mA防護(hù)等級(jí)IP65符合DIN40050，EN605291）p1>p2+10％p22）在p1-10巴到p2-6.3巴wilkerson90系列調(diào)節(jié)器技術(shù)理wilkerson調(diào)節(jié)器是調(diào)節(jié)電壓的裝置，首先防止電壓過(guò)高損壞電瓶，同時(shí)在發(fā)電機(jī)電壓過(guò)低時(shí)切斷與電瓶之間的線路，上面說(shuō)的是直流發(fā)電機(jī)。假如是交流發(fā)電機(jī)則只有穩(wěn)壓功能。具有3/4“＆1”集成端口（BSPP或NPT）的模塊化設(shè)計(jì)?提供安全的壓力引導(dǎo)?自動(dòng)轉(zhuǎn)儲(chǔ)下游的壓力導(dǎo)頻信號(hào)?慢啟動(dòng)可調(diào)?電磁閥或空氣先導(dǎo)選項(xiàng)?高流量和排氣能力wilkerson90系列調(diào)節(jié)器技術(shù)理[詳細(xì)]

  2018-09-21 10:00

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• C90-0 6-A H G M，wilkerson調(diào)節(jié)器

  C90-06-AHGM，wilkerson調(diào)節(jié)器wilkerson調(diào)節(jié)器產(chǎn)品特性集成3/4“或1”端口（NPT和BSPP）?GX顆粒元素作為標(biāo)準(zhǔn)?優(yōu)異的除水效率?堅(jiān)固而輕便的鋁結(jié)構(gòu)?低溫-40°，手動(dòng)/半自動(dòng)組合流失為標(biāo)準(zhǔn)C90-06-AHGM，wilkerson調(diào)節(jié)器wilkerson調(diào)節(jié)器產(chǎn)品規(guī)格流體壓縮空氣Zda入口壓力*17.5bar（254psig）溫度范圍*：自動(dòng)排水組合排水-10°C至60°C（14°F至140°F）-40°C至60°C（-40°F至140°F）顆粒去除5微米空氣質(zhì)量符合ISO8573-1：1991第3類和第5類（顆粒物）ISO8573-1：2001第6類和第7類（顆粒物）典型流量5微米元素6.3bar（91.4psig）入口壓力和0.5巴（7.3psig）壓降1“端口170scfm手動(dòng)/半自動(dòng)排水關(guān)閉0.8bar（11.6psig）G1/8螺紋公汽車排水碗壓力關(guān)閉排水0.8bar（11.6psig）工作范圍手動(dòng)覆蓋設(shè)施0.8巴（11.6磅/平方英寸）至17.5巴（254psig）碗容量130cm3（4.4USoz）材料規(guī)格身體鋁鏡玻璃聚丙烯車身罩ABS元素?zé)Y(jié)密封腈橡膠排水手動(dòng)/半自動(dòng)：縮醛自動(dòng)：PA/?10mm黃銅C90-06-AHGM，wilkerson調(diào)節(jié)器[詳細(xì)]

  2018-09-21 10:00

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• wilkerson威爾克森過(guò)濾減壓閥說(shuō)明書

  wilkerson威爾克森過(guò)濾減壓閥說(shuō)明書wilkerson威爾克森調(diào)壓閥作用定位器，就是信號(hào)壓力增加，輸出壓力亦增加；所謂反作用定位器，就是信號(hào)壓力增加，輸出壓力則減少。一臺(tái)正作用執(zhí)行機(jī)構(gòu)只要裝上反作用定位器，就能實(shí)現(xiàn)反作用執(zhí)行機(jī)構(gòu)的動(dòng)作；相反，一臺(tái)反作用執(zhí)行機(jī)構(gòu)只要裝上反作用定位器，就能實(shí)現(xiàn)正作用執(zhí)行機(jī)構(gòu)的動(dòng)作。wilkerson威爾克森調(diào)壓閥調(diào)節(jié)閥通常由電動(dòng)執(zhí)行機(jī)構(gòu)或氣動(dòng)執(zhí)行機(jī)構(gòu)與閥體兩部分共同組成。直行程主要有直通單座式和直通雙座式兩種，后者具有流通能力大、不平衡辦小和操作穩(wěn)定的特點(diǎn)，所以通常特別適用于大流量、高壓降和泄漏少的場(chǎng)合。角行程主要有：V型電動(dòng)調(diào)節(jié)球閥、電動(dòng)蝶閥、通風(fēng)調(diào)節(jié)閥、偏心蝶閥等。wilkerson威爾克森調(diào)壓閥基本可以說(shuō)是減壓閥，減壓閥的工作原理如下：高壓介質(zhì)通過(guò)一個(gè)小孔充到一個(gè)相對(duì)較大的腔里實(shí)現(xiàn)減壓，實(shí)際上是靠截流減壓，膜片或活塞的兩面一面是出口腔，一面是人為給的壓力，并且控制小孔大小的閥桿和膜片(活塞)相連，這樣只要給一個(gè)固定的壓力，那么出口腔的壓力就會(huì)一直等于這個(gè)壓力，這個(gè)認(rèn)為給定的壓力可以有彈簧或氣源或液壓源來(lái)提供。wilkerson威爾克森調(diào)壓閥不減壓，容易造成膠管脫落，泄漏燃?xì)?，后果不堪設(shè)想。減壓原理：高壓介質(zhì)通過(guò)一個(gè)小孔充到一個(gè)相對(duì)較大的腔里實(shí)現(xiàn)減壓，實(shí)際上是靠截流減壓。膜片或活塞的兩面一面是出口腔，一面是人為給的壓力，并且控制小孔大小的閥桿和膜片（活塞）相連。威爾克森調(diào)壓閥R21-C3-L00|調(diào)壓閥R21-C6-000|調(diào)壓閥R11-C2-000|調(diào)壓閥R41-CC-000|調(diào)壓閥M16-02-F00|調(diào)壓閥M26-03-F00wilkerson威爾克森過(guò)濾減壓閥說(shuō)明書上海威斯特您提供美國(guó)wilkersoncorp顆粒過(guò)濾器、美國(guó)wilkersoncorp凝聚式過(guò)濾器、美國(guó)wilkersoncorp后過(guò)濾器、美國(guó)wilkersoncorp調(diào)節(jié)器、美國(guó)wilkersoncorp高精密穩(wěn)壓器、美國(guó)wilkersoncorp潤(rùn)滑器、美國(guó)wilkersoncorp過(guò)濾器/調(diào)節(jié)閥、美國(guó)wilkersoncorp組合電器、美國(guó)wilkersoncorp干燥器、美國(guó)wilkersoncorp不銹鋼鋼制FRLs、美國(guó)wilkersoncorp配件及維修工具包等等。下面我為大家介紹一下wilkerson威爾克森調(diào)壓閥作用的說(shuō)明書：wilkerson威爾克森調(diào)壓閥根據(jù)使用特點(diǎn)分：緊湊型減壓閥、中等流量減壓閥、常規(guī)高壓減壓閥、二級(jí)減壓閥、低壓大流量減壓閥。wilkerson威爾克森調(diào)壓閥其工作原理是按力平衡原理設(shè)計(jì)和工作的。當(dāng)通入波紋管的信號(hào)壓力增加時(shí)，使杠桿2繞支點(diǎn)轉(zhuǎn)動(dòng)，檔板靠近噴嘴，噴嘴背壓經(jīng)放大器放大后，送入薄膜執(zhí)行機(jī)構(gòu)氣室，使閥桿向下移動(dòng)，并帶動(dòng)反饋桿（擺桿）繞支點(diǎn)轉(zhuǎn)動(dòng)，連接在同一軸上的反饋凸輪（偏心凸輪）也跟著作逆時(shí)針?lè)较蜣D(zhuǎn)動(dòng)，通過(guò)滾輪使杠桿1繞支點(diǎn)轉(zhuǎn)動(dòng)，并將反饋彈簧拉伸、彈簧對(duì)杠桿2的拉力與信號(hào)壓力作用在波紋管上的力達(dá)到力矩平衡時(shí)儀表達(dá)到平衡狀態(tài)。此時(shí)，一定的信號(hào)壓力就與一定的閥門位置相對(duì)應(yīng)。以上作用方式為正作用，若要改變作用方式，只要將凸輪翻轉(zhuǎn)，A向變成B向等，即可。wilkerson威爾克森過(guò)濾減壓閥說(shuō)明書[詳細(xì)]

  2018-09-27 10:00

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• 國(guó)家ZD新產(chǎn)品

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  2003-04-07 00:00

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• 歐司朗LED新產(chǎn)品

  歐司朗2014年新產(chǎn)品。[詳細(xì)]

  2018-10-30 10:00

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• socorex移液器新產(chǎn)品

  瑞士SOCOREX成立于1940年，是從事高精密液體操作產(chǎn)品的研發(fā)，生產(chǎn)及經(jīng)營(yíng)的專業(yè)廠家。配備完善的技術(shù)支持，維修和校準(zhǔn)服務(wù)。產(chǎn)品包括：手動(dòng)，電動(dòng)移液器，多道移液器，瓶口配液器、移液管控制器、連續(xù)分液器、連續(xù)注射器，特殊用途移液器及各產(chǎn)品完備的附件系統(tǒng)。可以實(shí)現(xiàn)液體計(jì)量、定量給料，轉(zhuǎn)移傳輸、等量分配和液體注射等多項(xiàng)液體操作功能。瑞士SOCOREX公司通過(guò)對(duì)精密液體操作產(chǎn)品及優(yōu)質(zhì)服務(wù)的高度關(guān)注，通過(guò)了ISO9001和ISO13485質(zhì)量管理體系認(rèn)證。所有產(chǎn)品通過(guò)ISO8655，GLP，GMP和NCCLS認(rèn)證文件，同時(shí)所有產(chǎn)品出廠前進(jìn)行嚴(yán)格QC認(rèn)證，每個(gè)產(chǎn)品都有單獨(dú)出具的報(bào)告文件。SOCOREX每個(gè)精密儀器具備有其自身的序列號(hào)并通過(guò)了嚴(yán)格的品控測(cè)試，獨(dú)立的第三方認(rèn)證?！耙徊绞浇鉀Q方案”時(shí)SOCOREX服務(wù)ZX的格言。依據(jù)ISO17025標(biāo)準(zhǔn)，SOCOREX服務(wù)ZX提供了性的維修和校準(zhǔn)程序。[詳細(xì)]

  2024-10-10 02:50

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• FRITSCH加強(qiáng)型新產(chǎn)品簡(jiǎn)介

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  2016-05-02 00:00

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• BioCheck新產(chǎn)品BC-1303 說(shuō)明書

  世界ding級(jí)實(shí)驗(yàn)材料供應(yīng)商BioCheck正式授權(quán)上海起發(fā)為其ZG代理，BioCheck在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來(lái)**的產(chǎn)品，**的服務(wù)，簽約BioCheck就是為了給廣大科研客戶帶來(lái)更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck廣東代理，BioCheck江蘇代理BioCheck湖北代理,BioCheck天津,BioCheck黑龍江代理,BioCheck內(nèi)蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江蘇代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由創(chuàng)始人Dr.JohnChen,Medix創(chuàng)立,是一家抓也提供腫瘤標(biāo)志物，心肌標(biāo)志物，激素類Z高性價(jià)比的抗體的公司。簡(jiǎn)要原理利用競(jìng)爭(zhēng)酶聯(lián)免疫方法，預(yù)先在微孔中包被羊抗兔抗體，實(shí)驗(yàn)時(shí)先后加入氯霉素標(biāo)準(zhǔn)品或待測(cè)樣本，氯霉素酶標(biāo)抗原和兔抗氯霉素抗體。經(jīng)過(guò)室溫溫育，反應(yīng)液中的兔抗氯霉素抗體與微孔板上的羊抗兔抗體結(jié)合，待測(cè)樣品中的抗原與氯霉素酶標(biāo)抗原競(jìng)爭(zhēng)微孔板上的兔抗氯霉素抗體。洗滌后，沒(méi)有與抗體結(jié)合的待測(cè)樣品中的抗原或酶標(biāo)抗原被洗去，再加入反應(yīng)底物，結(jié)合的酶標(biāo)抗原的酶將底物轉(zhuǎn)化為藍(lán)色產(chǎn)物，加入終止液后顏色由藍(lán)色變?yōu)辄S色。反應(yīng)完成后，樣品中氯霉素含量越多，反應(yīng)呈色就越淺；反之，樣品中氯霉素含量越少，則呈色越深。利用標(biāo)準(zhǔn)曲線可計(jì)算出樣品中氯霉素含量。技術(shù)參數(shù)檢測(cè)蜂蜜、蛋類、牛奶、奶粉、水產(chǎn)品、動(dòng)物組織(肌肉、肝臟等)、飼料、血清、血漿及尿液樣本中存在的氯霉素，定量限可達(dá)0.025ppb。No.樣品檢測(cè)下限1蜂蜜0.02ppb(0.02ng/g)2蛋類0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5蝦，魚及肉類0.08ppb(0.08ng/g)6飼料0.08ppb(0.08ng/g)7血清/血漿0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反應(yīng)名稱百分比氯霉素堿0.4%甲基氯霉素<0.04%回收率No.樣品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，蝦，魚及肉類95%~120%4飼料95%~120%5血清/血漿90%~120%6尿液1**%~130%PD-L1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1303EnzymeImmunoassayfortheQuantitativeDeterminationofPD-L1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammeddeathreceptor-ligand1(PD-L1,B7-H1,CD274)isabiomarkerfromtheB7familythatisexpressedonavarietyofcellsandupregulatedinresponsetopro-inflammatorycytokinessuchasinterferongamma1,2,3.PD-L1interactswithPD-1,aco-receptorexpressedbyexhaustedTcells,toencourageanimmunosuppressivetumormicroenvironmentbydecreasingTcellreceptormediatedproliferationandcytokineproduction4,5.ThePD-1/PD-L1interactionfunctionsasanimmunecheckpointinaprocessknownasimmunoeditingwherethehostimmunesystemeliminateshighlyimmunogenictumorswhileallowinglessimmunogenictumortoevadeit2,6.Multiplesolidtumortypesincludingmelanoma,renalcellcarcinoma,non-smallcelllungcancer,ovarian,andcolorectalcancerutilizethisPD-1/PD-L1immunoeditingmechanism2.CurrenttreatmenthasfocusedonblockingthePD-1/PD-L1interactiontoreducetumorevasionbyinhibitingPD-1,withnewfocusonPD-L1aswell1,2.PRINCIPLEOFTHEASSAYThePD-L1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-L1molecule.Onemousemonoclonalanti-PD-L1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-L1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-L1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-L1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-L120ng/mlPD-L1Standard(0.5mL/vial)20ng/mLPD-L1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-L1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute20ng/mLstandardto5ng/mL.Preparetwo-foldserialdilutionsofthe5ng/mLStandardwithStandard/SampleDiluent:5ng/mL:0.15mLof20ng/mL+0.45mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0.078ng/mL:0.25mLof0.156ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-L1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-L1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-L1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-L1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-L1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-L1Absorbance(ng/ml)(450nm)00.0420.0780.1410.1560.2360.3130.4150.6250.7261.251.2832.52.10053.053nm3.53(4502.52Absorbance1.510.50012345PD-L1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-L1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.02ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-L1(ng/mL)1.53.07.4S.D.0.040.050.18C.V.(%)2.4%1.6%2.4%2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-L1(ng/mL)1.63.07.4S.D.0.040.120.26C.V.(%)2.5%4.1%3.5%RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-L1levelsandassayedinduplicate.Themeanrecoverywas90%.SampleEXPECTEDOBSERVED%RECOVERY[PD-L1][PD-L1](ng/mL)(ng/mL)121.890%254.692%3108.989%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas110.3%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:41.61.6N/A1:81.7106.3%1:161.7106.3%1:321.8112.5%Mean=108.4%2.1:43.73.7N/A1:84.0108.1%1:164.0108.1%1:324.1110.8%Mean=109.0%3.1:47.47.4N/A1:88.3112.2%1:168.6116.2%1:328.3112.2%Mean=113.5%REFERENCESHerbst,RoyS.,etal."Predictivecorrelatesofresponsetotheanti-PD-L1antibodyMPDL3280Aincancerpatients."Nature515.7528(2014):563.Patel,SandipPravin,andRazelleKurzrock."PD-L1expressionasapredictivebiomarkerincancerimmunotherapy."Molecularcancertherapeutics14.4(2015):847-856.Topalian,SuzanneL.,CharlesG.Drake,andDrewM.Pardoll."TargetingthePD-1/B7-H1(PD-L1)pathwaytoactivateanti-tumorimmunity."Currentopinioninimmunology24.2(2012):207-212.Blank,Christian,andAndreasMackensen."ContributionofthePD-L1/PD-1pathwaytoT-cellexhaustion:anupdateonimplicationsforchronicinfectionsandtumorevasion."Cancerimmunology,immunotherapy56.5(2007):739-745.Barber,DanielL.,etal."RestoringfunctioninexhaustedCD8Tcellsduringchronicviralinfection."Nature439.7077(2006):682.Teng,MicheleWL,etal."ClassifyingcancersbasedonT-cellinfiltrationandPD-L1."Cancerresearch75.11(2015):2139-2145.部分Zxin產(chǎn)品如下：貨號(hào)品名Tests/Kit規(guī)格品牌70748MouseMonoclonalanti-humanPD-1(Capture)Purified0.1/0.5/1mgBioCheck70749MouseMonoclonalanti-humanPD-1(Capture)Purified0.1/0.5/1mgBioCheck70750MouseMonoclonalanti-humanPD-1(Detection)Purified0.1/0.5/1mgBioCheck70751MouseMonoclonalanti-humanPD-L1(Capture)Purified0.1/0.5/1mgBioCheck70752MouseMonoclonalanti-humanPD-L1(Capture)Purified0.1/0.5/1mgBioCheck70753MouseMonoclonalanti-humanPD-L1(Detection)Purified0.1/0.5/1mgBioCheck70745Mousemonoclonalanti-humanLp-PLA2(Capture)Purified0.5mgBioCheck70746Mousemonoclonalanti-humanLp-PLA2(Detection)Purified0.5mgBioCheck70747Mousemonoclonalanti-humanLp-PLA2(Detection)Purified0.5mgBioCheck我們公司Zda優(yōu)勢(shì)是強(qiáng)大的采購(gòu)，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營(yíng)過(guò)700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢(shì)，3：提供加急服務(wù)，一般1-2周到貨，超過(guò)時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢(shì)，當(dāng)然不能保證1**%產(chǎn)品都是價(jià)格Zdi,因?yàn)閮r(jià)格Zdi意味著沒(méi)有服務(wù).5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國(guó)藥，fisher等500多家公司6：我們du家代理的品牌有：AntibodyResearchCorporation，arcticzymes，Biorelevant，AmberGen,Inc.，clemente-associates，clodronateliposomes，ColumbiaBiosciences，enzymeresearch，GeneBridgesGmbH，Genovis，AmberGen,Inc.BiotechnologyGmbH，HaematologicTechnologiesHTIHaemtech，hookelabs，Immudex，InnovativeResearchofAmerica，inspiralis，ListBiologicalLaboratories,Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-FreezBiologicals，pentapharm，progen，ProteinArk，QA-Bio,Inc，QA-Bio,IncQuickZymeBiosciences，Teknova，TriLinkBioTechnologies,Inc.，ZyagenLaboratories等7：我們還是invitrogen,qiagen,MidlandBioProductsCorporationam,sigma;neb,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā)，歡迎合作。[詳細(xì)]

  2018-09-29 10:02

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• BioCheck新產(chǎn)品BC-1301 說(shuō)明書

  世界ding級(jí)實(shí)驗(yàn)材料供應(yīng)商BioCheck正式授權(quán)上海起發(fā)為其ZG代理，BioCheck在一直是行業(yè)的標(biāo)桿,一直為廣大科研客戶提供Z為優(yōu)質(zhì)的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為ZG科研客戶帶來(lái)**的產(chǎn)品，**的服務(wù)，簽約BioCheck就是為了給廣大科研客戶帶來(lái)更加完善的產(chǎn)品和服務(wù),您的滿意將是我們Zda的收獲BioCheckZG代理,BioCheck上海代理，BioCheck北京代理，BioCheck廣東代理，BioCheck江蘇代理BioCheck湖北代理,BioCheck天津,BioCheck黑龍江代理,BioCheck內(nèi)蒙古代理,BioCheck吉林代理,BioCheck福建代理,BioCheck江蘇代理,BioCheck浙江代理,BioCheck四川代理BIOCHECK公司由創(chuàng)始人Dr.JohnChen,Medix創(chuàng)立,是一家抓也提供腫瘤標(biāo)志物，心肌標(biāo)志物，激素類Z高性價(jià)比的抗體的公司。簡(jiǎn)要原理利用競(jìng)爭(zhēng)酶聯(lián)免疫方法，預(yù)先在微孔中包被羊抗兔抗體，實(shí)驗(yàn)時(shí)先后加入氯霉素標(biāo)準(zhǔn)品或待測(cè)樣本，氯霉素酶標(biāo)抗原和兔抗氯霉素抗體。經(jīng)過(guò)室溫溫育，反應(yīng)液中的兔抗氯霉素抗體與微孔板上的羊抗兔抗體結(jié)合，待測(cè)樣品中的抗原與氯霉素酶標(biāo)抗原競(jìng)爭(zhēng)微孔板上的兔抗氯霉素抗體。洗滌后，沒(méi)有與抗體結(jié)合的待測(cè)樣品中的抗原或酶標(biāo)抗原被洗去，再加入反應(yīng)底物，結(jié)合的酶標(biāo)抗原的酶將底物轉(zhuǎn)化為藍(lán)色產(chǎn)物，加入終止液后顏色由藍(lán)色變?yōu)辄S色。反應(yīng)完成后，樣品中氯霉素含量越多，反應(yīng)呈色就越淺；反之，樣品中氯霉素含量越少，則呈色越深。利用標(biāo)準(zhǔn)曲線可計(jì)算出樣品中氯霉素含量。技術(shù)參數(shù)檢測(cè)蜂蜜、蛋類、牛奶、奶粉、水產(chǎn)品、動(dòng)物組織(肌肉、肝臟等)、飼料、血清、血漿及尿液樣本中存在的氯霉素，定量限可達(dá)0.025ppb。No.樣品檢測(cè)下限1蜂蜜0.02ppb(0.02ng/g)2蛋類0.02ppb(0.02ng/g)3牛奶0.002ppb(0.002ng/ml)4奶粉0.012ppb(0.012ng/g)5蝦，魚及肉類0.08ppb(0.08ng/g)6飼料0.08ppb(0.08ng/g)7血清/血漿0.02ppb(0.02ng/ml)8尿液0.04ppb(0.04ng/ml)交叉反應(yīng)名稱百分比氯霉素堿0.4%甲基氯霉素<0.04%回收率No.樣品回收率1蜂蜜70%~110%2牛奶90%~130%3蛋，蝦，魚及肉類95%~120%4飼料95%~120%5血清/血漿90%~120%6尿液1**%~130%PD-1ENZYMEIMMUNOASSAYTESTKITCatalogNumber:BC-1301EnzymeImmunoassayfortheQuantitativeDeterminationofPD-1ConcentrationinHumanSerumandPlasmaFORRESEARCHUSEONLYNotforuseindiagnosticproceduresINTRODUCTIONProgrammedcelldeathprotein1,(PD-1,CD279,PDCD1),isacellsurfacereceptorthatiscriticalfortheregulationofTcellinflammatoryactivityandmaintainsperipheraltoleranceintheimmunesystem(1).WithanextracellularIgVdomain,atransmembranedomain,andanintracellulartailwithtwophosphorylationsites(SHP-1andSHP-2),PD-1isabletointeractwithligandsPD-L1andPD-L2todown-regulatetheimmunesystemandsuppressTcellactivity(2).Uponantigenrecognition,anactivatedTcellexpressesPD-1onitssurfaceandproducesinterferons,whichinducesexpressionofPD-L1,therebypromotingapoptosisorcelldeath.(3)Howeverwhenhijackedbytumorcells,aberrantexpressionofPD-1ligandsinhibitsimmune-modulatoryTcellactivation,leadingtodiseaseprogression(4).ElevatedlevelsofPD-1inserumandplasmahavebeenassociatedwithrheumatoidarthritisandskinsclerosis(5,6).Also,PD-1ispredominantlyexpressedbytumorinfiltratingTcells(7).FurtherresearchimpliesthatusingmonoclonalantibodiestotargetthePD-1immunologiccheckpointhascontributedtobreakthroughprogressinunderstandingandtreatingcancerssuchasmelanomaandothervariousnon-smallcelllungcancer(4).PRINCIPLEOFTHEASSAYThePD-1ELISAisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.TheassaysystemutilizesauniquemonoclonalantibodypairdirectedagainstadistinctantigenicdeterminantonthePD-1molecule.Onemousemonoclonalanti-PD-1antibodyisusedforsolidphaseimmobilization(onthemicrotiterwells).Anothermousemonoclonalanti-PD-1antibodyconjugatedtohorseradishperoxidase(HRP)isintheenzymeconjugatesolution.Thetestsamplesareallowedtoreactsequentiallywiththetwoantibodies,resultinginthePD-1moleculestobesandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Aftertwoseparate60-minuteincubationstepsatroomtemperature,thewellsarerinsedwithWashBuffertoremoveunboundlabeledantibodies.TMBReagentisaddedandincubatedfor30minutesunderdarkconditions,resultinginthedevelopmentofabluecolor.ThecolordevelopmentisstoppedwiththeadditionofStopSolution,changingthecolortoyellow.TheconcentrationofPD-1isdirectlyproportionaltothecolorintensityofthetestsamples.Absorbanceismeasuredspectrophotometricallyat450nm.REAGENTSANDMATERIALSPROVIDEDAntibody-CoatedWells(1plate,96wells)Microtiterwellscoatedwithmousemonoclonalanti-PD-150ng/mlPD-1Standard(0.5mL/vial)50ng/mLPD-1inphosphatebuffer-BSAsolutionwithpreservatives3.StandardandSampleDiluent(30mL/bottle,1bottle)Containsphosphatebuffer-BSAsolutionwithpreservativesEnzymeConjugateReagent(12mL/vial,1vial)Containsmousemonoclonalanti-PD-1conjugatedtohorseradishperoxidase20XWashBuffer(50mL/bottle,1bottle)PhosphatebufferwithdetergentsTMBReagent(11mL/bottle,1bottle)Containsone-stepTMBsolutionStopSolution(11mL/bottle,1bottle)Containsdilutedhydrochloricacid(1NHCl)STORAGECONDITIONSStoretheunopenedkitat2-8°Cuponreceiptandwhenitisnotinuse,untiltheexpirationshownonthekitlabel.Refertothepackagelabelfortheexpirationdate.Keepmicrotiterplateinasealedbagwithdesiccanttominimizeexposuretodampair.REAGENTPREPARATIONAllreagents首ldbeallowedtoreachroomtemperature(18-25°C)beforeuse.Foreachtestrun,prepareafreshstandardset.Dilute50ng/mLto10ng/mLwithStandard/Samplediluent.Preparetwo-foldserialdilutionsofthe10ng/mLStandardwithStandard/SampleDiluent:10ng/mL:0.12mLof50ng/mL+0.48mLofStandard/SampleDiluent5ng/mL:0.25mLof10ng/mL+0.25mLofStandard/SampleDiluent2.5ng/mL:0.25mLof5ng/mL+0.25mLofStandard/SampleDiluent1.25ng/mL:0.25mLof2.5ng/ml+0.25mLofStandard/SampleDiluent0.625ng/mL:0.25mLof1.25ng/mL+0.25mLofStandard/SampleDiluent0.313ng/mL:0.25mLof0.625ng/mL+0.25mLofStandard/SampleDiluent0.156ng/mL:0.25mLof0.313ng/mL+0.25mLofStandardDiluent0ng/mL:0.25mLofStandardDiluentPatientsamplesneedtobediluted4-foldpriortouse.Prepareaseriesofsmalltubes(i.e.,1.5mLmicrocentrifugetubes)andmix60Lofserumwith180LStandard/SampleDiluent.WorkingWashBuffer:Preparationof1XWashBufferfrom20XStock.Add50mLof20XWashBufferStockto950mLofDIH2O.TheWorkingWashBufferisstableat2-8°Cfor30days.NOTE:Anycrystalsthatmaybepresentduetohighsaltconcentrationmustberedissolvedatroomtemperaturebeforemakingthedilution.ASSAYPROCEDUREPrepareStandards.SeeReagentPreparation.Dilutesamples1:4dilution.SeeReagentPreparation.Securethedesirednumberofcoatedwellsintheholder.Dispense100mLofPD-1standards,andDILUTEDspecimensintotheappropriatewells.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300mLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLofPD-1WorkingEnzymeConjugateReagentintoeachwell.Incubatefor60minutesatroomtemperature(18-25°C).Removeincubationmixturebyflickingplatecontentsintoawastecontainer.Rinseandflickthemicrotiterwells5timeswith300uLWorkingWashBuffer.Strikethewellsontoabsorbentpaperorpapertowelstoremoveallresidualwaterdroplets.Dispense100mLTMBsolutionintoeachwell.Incubatefor30minutesatroomtemperature(18-25°C).Stopthereactionbyadding100mLofStopSolutionintoeachwell.Gentlymixfor30seconds.Itisimportanttomakesurethatallthebluecolorchangestoyellowcolorcompletely.Readabsorbanceat450nmwithamicrotiterwellreaderwithin15minutes.CALCULATIONOFRESULTSCalculatethemeanabsorbancevalue(OD450)foreachsetofreferencestandards,controlsandsamples.Constructastandardcurvebyplottingthemeanabsorbanceobtainedforeachreferencestandardagainstitsconcentrationinng/mlongraphpaper,withabsorbanceonthevertical(y)axisandconcentrationonthehorizontal(x)axis.ThecorrespondingconcentrationofPD-1(ng/mL)canbedeterminedfromthestandardcurveusingthemeanabsorbancevalueforeachsample.Dependingonexperienceand/ortheavailabilityofcomputercapability,othermethodsofdatareductionmaybeemployed.Theobtainedvaluesofthesamples首ldbemultipliedbythedilutionfactorof4toobtainPD-1resultsinng/ml.EXAMPLEOFSTANDARDCURVEResultsofatypicalstandardrunwithabsorbencyreadingsat450nmshownontheYaxisagainstPD-1concentrationsshownontheXaxis.NOTE:Thisstandardcurveisforthepurposeofillustrationonly,and首ldnotbeusedtocalculateunknowns.Eachlaboratorymustgenerateitsowndataandstandardcurveineachexperiment.PD-1Absorbance(ng/ml)(450nm)00.0890.1560.1380.3130.1900.6250.2841.250.4812.50.85151.581102.776nm32.5(4502Absorbance1.510.500246810PD-1Conc.(ng/ml)PERFORMANCECHARACTERISTICSSensitivityTheminimumdetectableconcentrationofthePD-1ELISAassayasmeasuredby2SDfromthemeanofazerostandardisestimatedtobe0.15ng/ml.PrecisionIntra-AssayPrecisionWithin-runprecisionwasdeterminedbyreplicatedeterminationsofthreedifferentsamplesinoneassay.Within-assayvariabilityisshownbelow:Sample123#Replicates242424MeanPD-1(ng/mL)8.413.222.9S.D.0.61.00.9C.V.(%)7.0%7.2%3.8%.2Inter-AssayPrecisionBetween-runprecisionwasdeterminedbyreplicatemeasurementsofthreedifferentsamplesoveraseriesofindividuallycalibratedassays.Between-assayvariabilityisshownbelow:Sample123#Replicates202020MeanPD-1(ng/mL)9.113.525.3S.D.0.81.10.9C.V.(%)9.2%7.93.7RecoveryandLinearityStudiesRecoverySampleswerespikedwithknownPD-1levelsandassayedinduplicate.Themeanrecoverywas105.3%.PAIREXPECTEDOBSERVED%RECOVERYNUMBER[PD-1][PD-1](ng/mL)(ng/mL)188.8110.0%212.513.1104.8%32525.3101.2%LinearityThreesampleswereseriallydilutedtodeterminelinearity.Themeanrecoverywas99.6%.#DilutionExpectedObservedConc.(ng/ml)Conc.(ng/ml)%Expected1.1:210.1114.8%1:48.88.8N/A1:87.888.6%Mean=101.7%2.1:214.5110.6%1:413.113.1N/A1:811.688.5%Mean=99.6%3.1:226.5104.7%1:425.325.3N/A1:823.090.0%Mean=97.4%REFERENCESFife,B.T.,&Pauken,K.E.(2011).TheroleofthePD-1pathwayinautoimmunityandperipheraltolerance.AnnalsoftheNewYorkAcademyofSciences,1217(1),45-59.Riella,L.V.,Paterson,A.M.,Sharpe,A.H.,&Chandraker,A.(2012).RoleofthePD-1PathwayintheImmuneResponse.AmericanJournalofTransplantation:OfficialJournaloftheAmericanSocietyofTransplantationandtheAmericanSocietyofTransplantSurgeons,12(10),25752587.Zak,Krzysztof&Kitel,Radosaw&Przetocka,Sara&Golik,Przemysaw&Guzik,Katarzyna&Musielak,Bogdan&Dm領(lǐng),Alexander&Dubin,Grzegorz&Holak,Tad.(2015).StructureoftheComplexofHumanProgrammedDeath1,PD-1,andItsLigandPD-L1.Structure.23..10.1016/j.str.2015.09.010.ZoranGatalica,CarrieSnyder,ToddManey,AnatoleGhazalpour,DanielHolterman,NianqingXiao,PeggyOverberg,IngaRose,GargiD.Basu,SemirVranic,HenryT.Lynch,DanielD.VonHoffandOmidHamid.ProgrammedCellDeath1(PD-1)andItsLigand(PD-L1)inCommonCancersandTheirCorrelationwithMolecularCancerType.CancerEpidemiolBiomarkersPrev.December12014(23)(12)2965-2970.Greisen,S.,Rasmussen,T.,Stengaard-Pedersen,K.,Hetland,M.,Hrslev-Petersen,K.,Hvid,M.,&Deleuran,B.(2013).Increasedsolubleprogrammeddeath-1(sPD-1)isassociatedwithdiseaseactivityandradiographicprogressioninearlyrheumatoidarthritis.ScandinavianJournalofRheumatology,43(2),101-108.Yanaba,K.,Hayashi,M.,Yoshihara,Y.,&Nakagawa,H.(2016).Serumlevelsofsolubleprogrammeddeath-1andprogrammeddeathligand-1insystemicsclerosis:Associationwithextentofskinsclerosis.TheJournalofDermatology,43(8),954-957.Ahmadzadeh,M.,Johnson,L.A.,Heemskerk,B.,Wunderlich,J.R.,Dudley,M.E.,White,D.E.,&Rosenberg,S.A.(2009).TumorantigenspecificCD8TcellsinfiltratingthetumorexpresshighlevelsofPD-1andarefunctionallyimpaired.Blood,114(8),15371544.部分產(chǎn)品Zxin報(bào)價(jià)如下：貨號(hào)品名規(guī)格價(jià)格品牌BC-1009AFPEIAKitkit3980BioCheckBC-1011CEAEIAKitkit4016BioCheckBC-1013CA125EIAKitkit4970BioCheckBC-1015CA15-3EIAKitkit4970BioCheckBC-1017CA19-9EIAKitkit4970BioCheckBC-1019PSAEIAKit*kit3962BioCheckBC-1021FreePSAEIAKit*kit4196BioCheckBC-1023Freeb-hCGEIAKitkit4196BioCheckBC-1061b-2MicroglobulinEIAKit§kit4250BioCheckBC-1001TSHEIAKit§kit3800BioCheckBC-1003U-TSH(S-TSH)EIAKit§kit3800BioCheckBC-1005T3EIAKit§kit3656BioCheckBC-1006FreeT3EIAKit§kit3890BioCheckBC-1007T4EIAKit§kit3656BioCheckBC-1008FreeT4EIAKitkit3890BioCheckBC-1081RubellaIgGEIAKit*kit4106BioCheckBC-1083RubellaIgMEIAKit*kit4196BioCheckBC-1085ToxoIgGEIAKit*kit3980BioCheckBC-1087ToxoIgMEIAKit*kit4070BioCheckBC-1089CMVIgGEIAKit*kit3980BioCheckBC-1091CMVIgMEIAKit*kit4070BioCheckBC-1093HSVIIgGEIAKitkit3980BioCheckBC-1095HSVIIgMEIAKitkit4196BioCheckBC-1097HSVIIIgGEIAKitkit3980BioCheckBC-1099HSVIIIgMEIAKitkit4070BioCheckBC-1051H.pyloriIgG(Qualitative)EIAKitkit4016BioCheckBC-1052H.pyloriIgG(Quantitative)EIAKitkit4106BioCheckBC-1053H.pyloriIgM(Qualitative)EIAKitkit4286BioCheckBC-1027hCGEIAKit§kit3800BioCheckBC-1029FSHEIAKit§kit3800BioCheckBC-1031LHEIAKit§kit3800BioCheckBC-1037ProlactinEIAKit§kit3800BioCheckBC-1025HumanFerritinEIAKit§kit3800BioCheckBC-1033HGHEIAKit§kit3800BioCheckBC-1035IgEEIAKit§kit3800BioCheckBC-1203AdiponectinEIAKit*kit7580BioCheckBC-1105TroponinIEIAKit§kit5330BioCheckBC-1117MyoglobinEIAKit§kit4250BioCheckBC-1119HS-CRPEIAKit§kit4250BioCheckBC-1121CK-MBEIAKit*kit6680BioCheckBC-1129MPOEIAKit*kit8480BioCheckBC-1111EstradiolEIAKit§kit4250BioCheckBC-1113ProgesteroneEIAKit§kit4250BioCheckBC-1115TestosteroneEIAKit§kit4250BioCheck我們公司Zda優(yōu)勢(shì)是強(qiáng)大的采購(gòu)，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營(yíng)過(guò)700多個(gè)品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢(shì)，3：提供加急服務(wù)，一般1-2周到貨，超過(guò)時(shí)限加急費(fèi)全免4：價(jià)格公道，絕大部分價(jià)格有優(yōu)勢(shì)，當(dāng)然不能保證1**%產(chǎn)品都有價(jià)格優(yōu)勢(shì)，5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國(guó)藥，fisher等500多家公司6：我們還是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc;Genebridege;GlycotopeBiotechnologyGmbH;iduron;InnovativeResearchofAmerica；Ludger；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；BIOCHECKINC-biochem;zyagen;......幾十家國(guó)外公司授權(quán)代理7：我們還從事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批發(fā)[詳細(xì)]

  2018-09-29 10:02

  產(chǎn)品樣冊(cè)

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