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pSecTag2 A&B&C說(shuō)明書
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pSecTag2A&B&C型號(hào)載體名稱出品公司載體用途VPI0036pSecTag2AInvitrogen哺乳動(dòng)物表達(dá)載體產(chǎn)品參數(shù):MammalianSelection:ZeocinSequencingPrimer:T7FwdSequencingPrimerSequence:5'd[TAATACGACTCACTATAGGG]3'Tag:6XHis,myc載體抗性:氨芐青霉素(Ampicillin)載體描述:pSecTag2andpSecTag2/Hygroaremammalianexpressionvectorsdesignedforthesecretion,purification,anddetectionoffusionproteins.EachvectorhasalargemultiplecloningsiteinthreereadingframestosimplifycloninginframewiththeN-terminalsecretionsignal.Thevectors(Figure1)offerthefollowingfeatures:SecretionsignalfromtheV-J2-CregionofthemouseIgkappa-chainforefficientsecretionofrecombinantproteins(Figure2)Cytomegalovirus(CMV)promoterforhigh-levelconstitutiveexpressionC-terminalpolyhistidine(6xHis)tagforrapidpurificationwithnickel-chelatingresinanddetectionwithanAnti-His(C-term)AntibodyC-terminalc-mycepitopefordetectionwithanAnti-mycAntibodyBovineGrowthHormone(BGH)polyadenylationsignalandtranscriptionterminationsequencetoenhancemRNAstabilitySV40originforepisomalreplicationandsimplevectorrescueincelllinesexpressingthelargeTantigen(e.g.,COS-1,COS-7)ThepSecTag2vectorscarrytheZeocinresistancegeneforcost-effectiveselectioninmammaliancells.ZeocinselectioncanalsobeusedinE.coli.ThepSecTag2/HygrovectorshavetheHygromycinBresistancegeneforselectionofstablemammaliancelllines.質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人:楊建輝400電話:4006551678辦公電話:021-50724187傳真:021-50724961*8006手機(jī):15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址:上海市浦東新區(qū)繡川路561號(hào)1102室
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pSecTag2 A&B&C說(shuō)明書
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說(shuō)明書
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