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pAc5.1/V5-His A&B&C
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2018-09-29 10:02 1711閱讀次數(shù)
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pAc5.1/V5-HisA&B&C型號載體名稱出品公司載體用途VGI0067pAc5.1V5-HisAInvitrogen果蠅細胞表達系統(tǒng)VGI0068pAc5.1V5-HisBInvitrogen果蠅細胞表達系統(tǒng)VGI0069pAc5.1V5-HisCInvitrogen果蠅細胞表達系統(tǒng)載體描述:TheDES-ConstitutiveKitprovidesthevectorpAc5.1/V5-Hisforhigh-levelconstitutiveexpressionofrecombinantproteinsandachoiceofBlasticidin（pCoBlast）orhygromycin（pCoHygro）selection.Thevectoroffersthestrong,constitutivepromoterfromtheDrosophilaactin5Cgene.ThepAc5.1/V5-Hisvectoralsooffersthefollowingfeatures:Smallsize（5.4kb）toimproveDNAyieldsandincreasesubcloningefficiency.C-terminalV5epitopetagforrapiddetectionwithInvitrogensAnti-Vntibody.C-terminal6xHistagforsimplepurificationofrecombinantfusionproteinsusingnickel-chelatingresin.Tofacilitatecloning,asetofthreevectors-A,B,andC-isprovided.EachvectorhasthemultiplecloningsiteinadifferentreadingframerelativetothecodingsequenceoftheC-terminaltag.質(zhì)粒圖譜:
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pAc5.1/V5-His A&B&C- pAc5.1/V5-HisA&B&C型號載體名稱出品公司載體用途VGI0067pAc5.1V5-HisAInvitrogen果蠅細胞表達系統(tǒng)VGI0068pAc5.1V5-HisBInvitrogen果蠅細胞表達系統(tǒng)VGI0069pAc5.1V5-HisCInvitrogen果蠅細胞表達系統(tǒng)載體描述:TheDES-ConstitutiveKitprovidesthevectorpAc5.1/V5-Hisforhigh-levelconstitutiveexpressionofrecombinantproteinsandachoiceofBlasticidin（pCoBlast）orhygromycin（pCoHygro）selection.Thevectoroffersthestrong,constitutivepromoterfromtheDrosophilaactin5Cgene.ThepAc5.1/V5-Hisvectoralsooffersthefollowingfeatures:Smallsize（5.4kb）toimproveDNAyieldsandincreasesubcloningefficiency.C-terminalV5epitopetagforrapiddetectionwithInvitrogensAnti-Vntibody.C-terminal6xHistagforsimplepurificationofrecombinantfusionproteinsusingnickel-chelatingresin.Tofacilitatecloning,asetofthreevectors-A,B,andC-isprovided.EachvectorhasthemultiplecloningsiteinadifferentreadingframerelativetothecodingsequenceoftheC-terminaltag.質(zhì)粒圖譜:[詳細]
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2018-09-29 10:02
產(chǎn)品樣冊
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- Invitrogen pGene v5-His 說明書
- GeneSwith哺乳動物誘導系統(tǒng)InvitrogenpGenev5-His產(chǎn)品參數(shù):MammalianSelection：ZeocinConstitutive/Inducible：InducibleExpressionLevelTightlycontrolled（activatewithmifepristone）SequencingPrimerpGeneFwdSequencingPrimerSequence：5'd[CTGCTATTCTGCTCAACCT]3'ProteinTags：6XHis，V5載體抗性:氨芐青霉素（Ampicillin）載體描述:Switch-OnExpressionfromtheLowestBasalLevelsTheGeneSwitchSystemforinduciblemammalianexpressionisidealforexperimentsthatrequiretheabsolutelowestuninducedexpressionlevels.TheexpressionvectorpGene/V5-Hisprovidesaminimalpromoter,GAL4-E1b,consistingofthebindingsitesfortheyeastGal4DNAbindingproteinfollowedbytheTATAsequencefromtheAdenovirusE1bpromoter.Withoutadditionalfactors,theGAL4-E1bpromoteristranscriptionallysilent.ToactivatetranscriptionfromtheGAL4-E1bpromoter,theGeneSwitchregulatoryproteinisexpressedfromaminimalTKpromoteronthepSwitchvector.TheGeneSwitchproteinhasthreefunctionaldomains:Gal4DNABindingDomain（Gal4-DBD）tobindtheregulatoryproteintotheGAL4-E1bpromoterTruncatedhumanProgesteroneReceptorLigandBindingDomain（hPR-LBD）thatundergoesaconformationalchangewhenitbindstheprogesteroneantagonist,mifepristoneTranscriptionactivationdomainfromtheNFBtranscriptionfactorp65（p6D）toactivatetranscriptionfromthesilentGAL4-E1bpromoterIntheabsenceofmifepristone,theconformationofthehPR-LBDregionpreventstheGeneSwitchregulatoryproteinfromactivatingtranscriptionfromtheGAL4-E1bpromoter.WhenmifepristoneisaddedandbindsthehPR-LBDregion,theGeneSwitchregulatoryproteinassumesaconformationthatpermitsittostimulatetranscriptionfromtheGAL4-E1bpromoter.InductionoftheGeneSwitchSystemleadstoactivationofthegeneofinterestonthevectorpGene/V5-His.Inaddition,fourGal4bindingsitesupstreamoftheminimalHSVTKpromoteronpSwitchcanbindthepSwitchregulatoryprotein.Therefore,addingmifepristoneup-regulatesproductionoftheregulatoryprotein.TheincreasedlevelsoftheGeneSwitchregulatoryproteinresultininductionofthegeneofinterestfrompGene/V5-Histolevelsthatcanapproachthoseofviralpromoters.TheGeneSwitchKitoffersexceptionallylowuninducedandhighinducedexpressionlevelsinmammaliancells.ThesystemprovidestheexpressionvectorpGene/V5-Hiswiththeminimal,syntheticGAL4-E1bpromoterthatistranscriptionallysilentuntilactivated.TheGeneSwitchregulatoryproteinbindstheGAL4-E1bpromotertoactivatetranscriptionupontheadditionofmifepristone.Inaddition,theGeneSwitchproteinupregulatesitsownexpression,leadingtoamplifiedexpressionofthegeneofinterestfrompGene/V5-His.pGene/V5-Hisoffersseveralfeaturesthatfacilitateexpressionanalysisandpurificationofrecombinantproteinsinmammaliancells:C-terminalV5epitopetagfordetectionwithAnti-VntibodiesC-terminalpolyhistidine（6xHis）tagforrapidpurificationwithnickel-chelatingresinanddetectionwithAnti-His（C-term）AntibodiesTheZeocinresistancegeneforeffectiveselectioninbothmammaliancellsandE.coli[詳細]
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2018-09-29 10:03
產(chǎn)品樣冊
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- pSecTag2 A&B&C說明書
- pSecTag2A&B&C型號載體名稱出品公司載體用途VPI0036pSecTag2AInvitrogen哺乳動物表達載體產(chǎn)品參數(shù):MammalianSelection：ZeocinSequencingPrimer：T7FwdSequencingPrimerSequence：5'd[TAATACGACTCACTATAGGG]3'Tag：6XHis，myc載體抗性:氨芐青霉素（Ampicillin）載體描述:pSecTag2andpSecTag2/Hygroaremammalianexpressionvectorsdesignedforthesecretion,purification,anddetectionoffusionproteins.EachvectorhasalargemultiplecloningsiteinthreereadingframestosimplifycloninginframewiththeN-terminalsecretionsignal.Thevectors（Figure1）offerthefollowingfeatures:SecretionsignalfromtheV-J2-CregionofthemouseIgkappa-chainforefficientsecretionofrecombinantproteins（Figure2）Cytomegalovirus（CMV）promoterforhigh-levelconstitutiveexpressionC-terminalpolyhistidine（6xHis）tagforrapidpurificationwithnickel-chelatingresinanddetectionwithanAnti-His（C-term）AntibodyC-terminalc-mycepitopefordetectionwithanAnti-mycAntibodyBovineGrowthHormone（BGH）polyadenylationsignalandtranscriptionterminationsequencetoenhancemRNAstabilitySV40originforepisomalreplicationandsimplevectorrescueincelllinesexpressingthelargeTantigen（e.g.,COS-1,COS-7）ThepSecTag2vectorscarrytheZeocinresistancegeneforcost-effectiveselectioninmammaliancells.ZeocinselectioncanalsobeusedinE.coli.ThepSecTag2/HygrovectorshavetheHygromycinBresistancegeneforselectionofstablemammaliancelllines.質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話：4006551678辦公電話：021-50724187傳真：021-50724961*8006手機：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號1102室[詳細]
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2018-09-29 10:02
產(chǎn)品樣冊
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- pCDNA4/HisMax A&B&C
- pCDNA4/HisMaxA&B&C型號載體名稱出品公司載體用途VPI0013pcDNA4/HisMaxBInvitrogen哺乳動物表達載體產(chǎn)品參數(shù):MammalianSelection：ZeocinSequencingPrimer：T7FwdSequencingPrimerSequence：5'd[TAATACGACTCACTATAGGG]3'Tag：6XHis，Xpress載體抗性:氨芐青霉素（Ampicillin）質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話：4006551678辦公電話：021-50724187傳真：021-50724961*8006手機：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號1102室[詳細]
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2018-09-29 10:02
產(chǎn)品樣冊
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- pcDNA3.1（-）/myc-His A&B&C說明書
- pcDNA3.1（-）/myc-HisA&B&C型號載體名稱出品公司載體用途VPI0017pcDNA3.1（-）/myc-HisAInvitrogen哺乳動物表達載體VPI0018pcDNA3.1（-）/myc-HisBInvitrogen哺乳動物表達載體VPI0019pcDNA3.1（-）/myc-HisCInvitrogen哺乳動物表達載體產(chǎn)品參數(shù):MammalianSelection：G418，neoProteinTagorFusion：c-MycEpitopeTag，HisTag（6xHis）SequencingPrimer：T7FwdSequencingPrimerSequence：5'd[TAATACGACTCACTATAGGG]3'載體抗性:氨芐青霉素（Ampicillin）質(zhì)粒圖譜:[詳細]
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2024-09-29 02:42
產(chǎn)品樣冊
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- Invitrogen四環(huán)素誘導系統(tǒng)（pCDNA4/HisMax A&B&C）說明書
- Invitrogen四環(huán)素誘導系統(tǒng)Vectors：pcDNA4/TO/Myc-HisA，pcDNA4/TO/Myc-HisB，pcDNA4/TO/Myc-HisC，產(chǎn)品參數(shù):MammalianSelection：ZeocinSequencingPrimer：T7FwdSequencingPrimerSequence：5'd[TAATACGACTCACTATAGGG]3'Tag：6XHis，Xpress載體抗性:氨芐青霉素（Ampicillin）質(zhì)粒圖譜:[詳細]
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2018-09-29 10:03
產(chǎn)品樣冊
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