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Optimization of β-carotene loaded solid lipid nanoparticles preparation using a high shear homogeniz
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Optimizationofβ-caroteneloadedsolidlipidnanoparticlespreparationusingahighshearhomogenizationtechnique
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Optimization of β-carotene loaded solid lipid nanoparticles preparation using a high shear homogeniz Optimizationofβ-caroteneloadedsolidlipidnanoparticlespreparationusingahighshearhomogenizationtechnique[詳細(xì)]
2018-08-31 10:11
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Preparation, in vitro and in vivo evaluation of polymeric nanoparticles bas hyaluronic acid-poly(but Herein,wedescribethepreparationofatargetedcellulardeliverysystemformorinhydrate(MH),-molecular-weighthyaluronicacid-poly(butylcyanoacrylate)(HA-PBCA)blockcopolymer.InordthetherapeuticeffectofMH,D-alpha-tocopherylpolyethyleneglycol1000succinate(TPGS)wasHA-PBCAduringthepreparationprocess.TheMH-loadedHA-PBCA“plain”nanoparticle(MH-PPBCA/TPGS“mixed”nanoparticles(MH-MNs)wereconcomitantlycharacterizedintermsofloadparticlesize,zetapotential,criticalaggregationconcentration,andmorphology.TheobtainedMHMNsexhibitedasphericalmorphologywithanegativezetapotentialandaparticlesizelessthan2favorablefordrugtargeting.Remarkably,theadditionofTPGSresultedinabout1.6-foldincreaseloading.TheinvitrocellviabilityexperimentrevealedthatMH-MNsenhancedthecytotoxicityofcellscomparedwithMHsolutionandMH-PNs.Furthermore,blankMNscontainingTPGSexhibitcytotoxiceffectsagainstcancercellswithoutdiminishingtheviabilityofnormalcells.Inaddition,uptakestudyindicatedthatMNsresultedin2.28-foldhighercellularuptakethanthatofPNs,inA5CD44receptorcompetitiveinhibitionandtheinternalizationpathwaystudiessuggestedthattheintmechanismofthenanoparticleswasmediatedmainlybytheCD44receptorsthroughaclathrin-dependocyticpathway.Moreimportantly,MH-MNsexhibitedahigherinvivoantitumorpotencyandtumorcellapoptosisthandidMH-PNs,followingintravenousadministrationtoS180tumor-bearinOverall,theresultsimplythatthedevelopednanoparticlesarepromisingvehiclesforthetargeteddlipophilicanticancerdrugs.Keywords:anti-tumoreffect,hyaluronicacid,TPGS,morinhydrate,nanoparticles[詳細(xì)]
2018-08-31 10:11
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2018-08-31 10:11
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α-胡蘿卜素（α-Carotene）酶聯(lián)免疫分析 試劑盒 α-胡蘿卜素（α-Carotene）酶聯(lián)免疫分析試劑盒檢測范圍：96T35pg/ml-1400pg/ml使用目的：用于測定玉米樣本中α-胡蘿卜素（α-Carotene）含量。α-胡蘿卜素（α-Carotene）酶聯(lián)免疫分析試劑盒實驗原理本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中α-胡蘿卜素（α-Carotene）水平。用純化的α-胡蘿卜素（α-Carotene）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入α-胡蘿卜素（α-Carotene），再與HRP標(biāo)記的α-胡蘿卜素（α-Carotene）抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成Z終的黃色。顏色的深淺和樣品中的α-胡蘿卜素（α-Carotene）呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中α-胡蘿卜素（α-Carotene）濃度。α-胡蘿卜素（α-Carotene）酶聯(lián)免疫分析試劑盒組成標(biāo)本要求1．標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實驗。若不能馬上進(jìn)行試驗，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融2．不能檢測含NaN3的樣品，因NaN3YZ辣根過氧化物酶的（HRP）活性。操作步驟1.標(biāo)準(zhǔn)品的稀釋：本試劑盒提供原倍標(biāo)準(zhǔn)品一支，用戶可按照下列圖表在小試管中進(jìn)行稀釋。2.加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑，其余各步操作相同）、標(biāo)準(zhǔn)孔、待測樣品孔。在酶標(biāo)包被板上標(biāo)準(zhǔn)品準(zhǔn)確加樣50μl，待測樣品孔中先加樣品稀釋液40μl，然后再加待測樣品10μl（樣品Z終稀釋度為5倍）。加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動混勻。3.溫育：用封板膜封板后置37℃溫育30分鐘。4.配液：將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用5.洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復(fù)5次，拍干。6.加酶：每孔加入酶標(biāo)試劑50μl，空白孔除外。7.溫育：操作同3。8.洗滌：操作同5。9.顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色10分鐘.10.終止：每孔加終止液50μl，終止反應(yīng)（此時藍(lán)色立轉(zhuǎn)黃色）。11.測定：以空白空調(diào)零，450nm波長依序測量各孔的吸光度（OD值）。測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。α-胡蘿卜素（α-Carotene）酶聯(lián)免疫分析試劑盒操作程序總結(jié)：計算以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo)，OD值為縱坐標(biāo)，在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線，根據(jù)樣品的OD值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計算出標(biāo)準(zhǔn)曲線的直線回歸方程式，將樣品的OD值代入方程式，計算出樣品濃度，再乘以稀釋倍數(shù)，即為樣品的實際濃度。注意事項1．試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開封后如未用完，板條應(yīng)裝入密封袋中保存。2．濃洗滌液可能會有結(jié)晶析出，稀釋時可在水浴中加溫助溶，洗滌時不影響結(jié)果。3．各步加樣均應(yīng)使用加樣器，并經(jīng)常校對其準(zhǔn)確性，以避免試驗誤差。一次加樣時間**控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。4．請每次測定的同時做標(biāo)準(zhǔn)曲線，**做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔**孔的OD值），請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定，計算時請Z后乘以總稀釋倍數(shù)（×n×5）。5．封板膜只限一次性使用，以避免交叉污染。6．底物請避光保存。7．嚴(yán)格按照說明書的操作進(jìn)行，試驗結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).8．所有樣品，洗滌液和各種廢棄物都應(yīng)按傳染物處理。9．本試劑不同批號組分不得混用。保存條件及有效期1．試劑盒保存：；2-8℃。2．有效期：6個月[詳細(xì)]
2018-11-04 10:00
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Optimization of β-carotene loaded solid lipid nanoparticles preparation using a high shear homogeniz
Shear Gel耦合劑
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