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Bone-Targeting Peptide and RNF146 Modified Apoptotic Extracellular Vesicles Alleviate Osteoporosis
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Bone-Targeting Peptide and RNF146 Modified Apoptotic Extracellular Vesicles Alleviate Osteoporosis
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Bone-Targeting Peptide and RNF146 Modified Apoptotic Extracellular Vesicles Alleviate Osteoporosis
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Characterization of stability, aggregation, and equilibrium properties of modified natural products
- Unstablebiopolymersolutionsinevitablyleadtomis-characterizationofmacromolecularpropertiesandirreproducibleresults.Evenunderstableorquasi-stableconditions,persistentaggregatescanhamperreliablecharacterization,especiallyusinglightscatteringmethods.Variouspitfallsincharacterizingbiopolymerswereworkedthrough,includingdeterminationofsolutionstabilityzones,dissolutionkinetics,estimationoffractionofaggregatepopulations,andtherelationshipbetweenbatchandfractionationmethods.Chitosans,polyampholyticbiopolymerswithisoelectricpointaroundpH=6.0,withvaryingdegreesofcarboxymethylationwerestudied.Instabilitywasdeterminedvs.pHandionicstrengthusingahighthroughputscreeningmethod,simultaneousmultiplesamplelightscattering(SMSLS).Withstablesolutionconditionsdetermined,equilibriumbatchandmulti-detectorGPCcharacterizationofmolecularweight,intrinsicviscosity,andpolyelectrolytepropertieswasmade.Finally,afirstattemptatcontinuousonlinemonitoringofthemodificationreactionitselfwasmadeandcomparedtoFTIRanalysisofcarboxymethylationondiscretealiquots.Giventherangeofpossiblecharacterizationproblems,multipleapproacheswithindependentinstrumentsmayberequiredforreliablenaturalproductcharacterization.Onlinemonitoringofmodificationreactionsmayleadtorapidadvancesinunderstandingandpreparationofnaturalproducts.[詳細(xì)]
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Characterization of stability, aggregation, and equilibrium properties of modified natural products;
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大豆多肽(peptide)酶聯(lián)免疫分析試劑盒使用說(shuō)明書(shū)
- 大豆多肽(peptide)酶聯(lián)免疫分析試劑盒使用說(shuō)明書(shū)本試劑盒僅供研究使用。檢測(cè)范圍:96T2ng/L-90ng/L使用目的:本試劑盒用于測(cè)定發(fā)酵豆粕樣本中大豆多肽(peptide)含量。實(shí)驗(yàn)原理本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中大豆多肽(peptide)水平。用純化的大豆多肽(peptide)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入大豆多肽(peptide),再與HRP標(biāo)記的大豆多肽(peptide)抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成Z終的黃色。顏色的深淺和樣品中的大豆多肽(peptide)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中大豆多肽(peptide)濃度。大豆多肽(peptide)酶聯(lián)免疫分析試劑盒組成標(biāo)本要求1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融2.不能檢測(cè)含NaN3的樣品,因NaN3YZ辣根過(guò)氧化物酶的(HRP)活性。大豆多肽(peptide)酶聯(lián)免疫分析試劑盒操作步驟1.標(biāo)準(zhǔn)品的稀釋:本試劑盒提供原倍標(biāo)準(zhǔn)品一支,用戶可按照下列圖表在小試管中進(jìn)行稀釋。2.加樣:分別設(shè)空白孔(空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、標(biāo)準(zhǔn)孔、待測(cè)樣品孔。在酶標(biāo)包被板上標(biāo)準(zhǔn)品準(zhǔn)確加樣50μl,待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl(樣品Z終稀釋度為5倍)。加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻。3.溫育:用封板膜封板后置37℃溫育30分鐘。4.配液:將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用5.洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。6.加酶:每孔加入酶標(biāo)試劑50μl,空白孔除外。7.溫育:操作同3。8.洗滌:操作同5。9.顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.10.終止:每孔加終止液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。11.測(cè)定:以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度(OD值)。測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。大豆多肽(peptide)酶聯(lián)免疫分析試劑盒操作程序總結(jié):計(jì)算以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)準(zhǔn)曲線的直線回歸方程式,將樣品的OD值代入方程式,計(jì)算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實(shí)際濃度。注意事項(xiàng)1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存。2.濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間**控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。4.請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,**做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高(樣本OD值大于標(biāo)準(zhǔn)品孔**孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)Z后乘以總稀釋倍數(shù)(×n×5)。5.封板膜只限一次性使用,以避免交叉污染。6.底物請(qǐng)避光保存。7.嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。9.本試劑不同批號(hào)組分不得混用。10.如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn)。保存條件及有效期1.試劑盒保存:2-8℃。2.有效期:6個(gè)月[詳細(xì)]
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2018-09-27 10:00
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半乳糖凝集素9蛋白galectin 9 peptide說(shuō)明書(shū)
- DESCRIPTIONSourceE.coliderivedAla2Thr323Accession#BAA31542NterminalSequenceAnalysisAla2Structure/FormMonomerPredictedMolecularMass35.8kDaSPECIFICATIONSSDSPAGE34kDa,reducingconditionsActivityMeasuredbyitsabilitytoinduceapoptosisofJurkathumanacuteTcellleukemiacells.Lu,L.H.etal.(2007)J.Biochem.141:157.Theforthiseffectistypically15ug/mL.Measuredbyitsabilitytoagglutinatehumanredbloodcells.Hadari,Y.R.etal.(2000)J.CellSci.113:2385.Theforthiseffectistypically2.5-12.5μg/mL.EndotoxinLevel<1.0EUper1μgoftheproteinbytheLALmethod.Purity>95%,bySDSPAGEunderreducingconditionsandvisualizedbysilverstain.FormulationLyophilizedfroma0.2μmfilteredsolutioninMOPS,NaCl,EDTA,DTTandTrehalose.SeeCertificateofAnalysisfordetails.PREPARATIONANDSTORAGEReconstitutionReconstituteat100μg/mLinwater.ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreezethawcycles.l12monthsfromdateofreceipt,20to70°Cassupplied.l1month,2to8°Cundersterileconditionsafterreconstitution.l3months,20to70°Cundersterileconditionsafterreconstitution.BACKGROUNDGalectinscompriseafamilyofmultifunctionalcarbohydratebindingproteinswithspecificityforN-acetyllactosaminecontainingglycoproteins.Atleast14mammalianGalectinssharestructuralsimilaritiesintheircarbohydraterecognitiondomains(CRD),formingthreegroups:prototype(oneCRD),tandemrepeat(twoCRDs),andchimeric(oneCRD,uniqueN-terminus)(1,2).FulllengthGalectin9isawidelyexpressed39kDatandemrepeatGalectinthatcontainstwoCRDsconnectedbyalinkerregion(3).Progressivedeletionwithinthelinkerregiongeneratesa36kDaisoform,alsoknownasEcalectinorUAT,aswellasa35kDaisoform(4).ThisrecombinantproteincorrespondstotheEcalectinisoformofhumanGalectin9andshares70%and73%aasequenceidentitywiththecorrespondingregionsofmouseandratGalectin9,respectively.Galectin9exhibitsawiderangeofactivities.Allthreeisoformsfunctionaseosinophilchemoattractants(5,6).Thisactivityisdestroyedbythrombinmediatedcleavagewithinthelinkerregionofthelongisoform,althoughtheEcalectinisoformisresistanttothrombin(7).Galectin9bindstocarbohydratemoietiesofIgE,therebypreventingimmunecomplexformation,mastcelldegranulation,andasthmaticandcutaneousanaphylaxisreactions(8).Independentofitslectinproperties,Galectin9inducesthematurationofdendriticcellswhichpromoteTh1polarization(9).Galectin9inducescellularapoptosisinpartbydirectbindingtoTIM3(10,11).ItsinteractionwithTIM3inhibitsTh1cellandCD8+cytotoxicTcellresponsesandalsopromotesregulatoryTcelldifferentiationandactivity(11,12).Galectin9suppressestumorcellmetastasisbyinterferingwiththeassociationsbetweenhyaluronicacidandCD44andbetweenVCAM1andIntegrinα4β1(13).TheEcalectinisoform(UATuratetransporter)canalsobeexpressedasanintegralmembraneproteinandmediatethecellulareffluxofurate(14).References:1.Yang,RY.etal.(2008)ExpertRev.Mol.Med.10:e17.2.Elola,M.T.etal.(2007)Cell.Mol.LifeSci.64:1679.3.Tureci,O.etal.(1997)J.Biol.Chem.272:6416.4.Chabot,S.etal.(2002)Glycobiology12:111.5.Matsumoto,R.etal.(2002)J.Immunol.168:1961.6.Sato,M.etal.(2002)Glycobiology12:191.7.Nishi,N.etal.(2006)Glycobiology16:15C.8.Niki,T.etal.(2009)J.Biol.Chem.284:32344.9.Dai,S.Y.etal.(2005)J.Immunol.175:2974.10.Seki,M.etal.(2007)ArthritisRheum.56:3968.11.Zhu,C.etal.(2005)Nat.Immunol.6:1245.12.Sehrawat,S.etal.(2010)PloSPathogens6:e1000882.13.Nobumoto,A.etal.(2008)Glycobiology18:735.14.LealPinto,E.etal.(2002)Am.J.Physiol.RenalPhysiol.283:F150.[詳細(xì)]
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Matrine derivate MASM uncovers a novel function for ribosomal protein S5 in osteoclastogenesis and postmenopausal osteoporosis
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Characterization of lgG Glycosylation Using Intact Protein Analysis and Peptide Mapping
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豬多肽YY(peptide YY)酶聯(lián)免疫分析試劑盒使用說(shuō)明書(shū)
- 豬多肽YY(peptideYY)酶聯(lián)免疫分析試劑盒使用說(shuō)明書(shū)本試劑盒僅供研究使用。檢測(cè)范圍:96T20pg/ml-480pg/ml使用目的:本試劑盒用于測(cè)定豬血清、血漿及相關(guān)液體樣本中多肽YY(peptideYY)含量。實(shí)驗(yàn)原理本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中豬多肽YY(peptideYY)水平。用純化的豬多肽YY(peptideYY)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入多肽YY(peptideYY),再與HRP標(biāo)記的多肽YY(peptideYY)抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成Z終的黃色。顏色的深淺和樣品中的多肽YY(peptideYY)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中豬多肽YY(peptideYY)濃度。試劑盒組成130倍濃縮洗滌液20ml×1瓶7終止液6ml×1瓶2酶標(biāo)試劑6ml×1瓶8標(biāo)準(zhǔn)品(960pg/ml)0.5ml×1瓶3酶標(biāo)包被板12孔×8條9標(biāo)準(zhǔn)品稀釋液1.5ml×1瓶4樣品稀釋液6ml×1瓶10說(shuō)明書(shū)1份5顯色劑A液6ml×1瓶11封板膜2張6顯色劑B液6ml×1/瓶12密封袋1個(gè)標(biāo)本要求1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融2.不能檢測(cè)含NaN3的樣品,因NaN3YZ辣根過(guò)氧化物酶的(HRP)活性。操作步驟標(biāo)準(zhǔn)品的稀釋:本試劑盒提供原倍標(biāo)準(zhǔn)品一支,用戶可按照下列圖表在小試管中進(jìn)行稀釋。480pg/ml5號(hào)標(biāo)準(zhǔn)品150μl的原倍標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液240pg/ml4號(hào)標(biāo)準(zhǔn)品150μl的5號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液120pg/ml3號(hào)標(biāo)準(zhǔn)品150μl的4號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液60pg/ml2號(hào)標(biāo)準(zhǔn)品150μl的3號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液30pg/ml1號(hào)標(biāo)準(zhǔn)品150μl的2號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液加樣:分別設(shè)空白孔(空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、標(biāo)準(zhǔn)孔、待測(cè)樣品孔。在酶標(biāo)包被板上標(biāo)準(zhǔn)品準(zhǔn)確加樣50μl,待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl(樣品Z終稀釋度為5倍)。加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻。溫育:用封板膜封板后置37℃溫育30分鐘。配液:將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。加酶:每孔加入酶標(biāo)試劑50μl,空白孔除外。溫育:操作同3。洗滌:操作同5。顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.終止:每孔加終止液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。測(cè)定:以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度(OD值)。測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。操作程序總結(jié):計(jì)算以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)準(zhǔn)曲線的直線回歸方程式,將樣品的OD值代入方程式,計(jì)算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實(shí)際濃度。注意事項(xiàng)1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存。2.濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間**控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,**做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高(樣本OD值大于標(biāo)準(zhǔn)品孔**孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)Z后乘以總稀釋倍數(shù)(×n×5)。封板膜只限一次性使用,以避免交叉污染。6.底物請(qǐng)避光保存。7.嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。9.本試劑不同批號(hào)組分不得混用。10.如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn)。保存條件及有效期1.試劑盒保存:;2-8℃。2.有效期:6個(gè)月[詳細(xì)]
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2018-09-14 10:00
產(chǎn)品樣冊(cè)
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使用 Agilent AdvanceBio Peptide Plus色譜柱分析合成多肽雜質(zhì)
- 通常,使用 UV 檢測(cè)的多肽色譜分離是通過(guò) C18 柱以及三氟乙酸 (TFA) 改性的流動(dòng) 相完成,這種方法可以改善分離度,但同時(shí)會(huì)YZ質(zhì)譜 (MS) 信號(hào)。甲酸 (FA) 是用 于 MS 檢測(cè)的流動(dòng)相改性劑,但它可能會(huì)導(dǎo)致傳統(tǒng) C18 柱無(wú)法達(dá)到**分離效 果。本應(yīng)用簡(jiǎn)報(bào)介紹了通過(guò)單一液相色譜方法,采用 Agilent AdvanceBio Peptide Plus 色譜柱分離合成多肽雜質(zhì),并使用 UV 或 MS 進(jìn)行檢測(cè)。本方法使用 FA 作為流 動(dòng)相改性劑,可以提供足夠的分離度以鑒定并定量多肽雜質(zhì)。[詳細(xì)]
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2020-05-08 14:54
應(yīng)用文章
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Injectable hydrogels with in situ-forming hydrophobic domains: oligo(D,L-lactide) modified poly(olig
- Injectable,insitu-gel領(lǐng)nanostructuredhydrogelshavebeenpreparedfromhydrazideandaldehydefunctionalizedpolymerprecursorsbasedonacopolymerofoligo(ethyleneglycol)methacrylate(OEGMA)andanoligo(lacticacid)macromonomer(OLA)withvaryinglacticacidchainlengths.Theresultinghydrogelscontainamixofchemical(hydrazonebondformationbetweenhydrazideandaldehydegroups)andphysical(hydrophobicinteractionsbetweenOLAchains)cross-linkswhichformcompetitivelyasafunctionoftheOLAchainlengthanddensity.AnincreaseintheOLAchainlengthanddensityresultsintheformationofmorephysicalcross-linksandfewerchemicalcross-links.Tuningtherelativeprevalenceofphysicalandchemicalcross-linkformationfacilitatedlargelyindependenttuningofgelmechanicsrelativetogelswel領(lǐng)anddegradation.Small-angleneutronscatteringoftheseOLA-containinghydrogelsrevealsamicrostructureconsistingofassociativehydrophobicdomains,basedonanincreasedscatteringintensityanddecreasedblobsizerelativetothatobservedforPOEGMAhydrogelspreparedwithouttheOLAco-monomer.ThepresenceofhydrophobicOLAdomainsincreasestheuptakeandslowsthereleaseofbovineserumalbumin,aproteinwell-knowntoassociatewithhydrophobicdomains.Coupledwiththeobservedcytocompatibilityofthereactiveprecursorpolymersusedtopreparethehydrogels,weanticipatesignificantpotentialapplicationsofthesehydrogelsfortheprolongedreleaseofhydrophobiccargoes.[詳細(xì)]
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2018-08-31 10:11
產(chǎn)品樣冊(cè)
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Thermodynamic Characterization of the Folding Coupled DNA Binding by the Monomeric Thrascription Activator GCN4 Peptide
- Thermodynamic Characterization of the Folding Coupled DNA Binding by the Monomeric Thrascription Activator GCN4 Peptide[詳細(xì)]
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2024-09-29 03:35
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