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儀器網(wǎng)> 資料庫>Clontech pTK-hyg (VSC0467)說明書

Clontech pTK-hyg (VSC0467)說明書

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2018-09-29 10:03 1517閱讀次數(shù)

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pTK-hyg型號(hào)載體名稱出品公司載體用途VSC0467pTK-hygClontech四環(huán)素調(diào)控系統(tǒng)DescriptionpTK-Hygisaselectionvectorthatconfershygromycinresistanceinmammaliancellsfortheselectionofstablytransformedcells.pTK-Hygisespeciallyusefulforselectionofdouble-stablecelllinesusingtheTet-OnorTet-OffGeneExpressionSystemsbecausetheabsenceofanenhancerelementonpTK-HygpreventstheunwantedactivationofpTRE-andpBI-derivedplasmidsuponcointegrationintothehostcell'sgenome.UsepTK-Hygcanbecotransfectedintothehostcelllinewithanexpressionplasmidthatcontainsageneofinterestusinganystandardtransfectiontechnique.Mostmammaliancelllinesrequire200g/mlofHygromycintoselectforstablytransformedcells.LocationofFeaturesHSVTKpolyAsignals:14731478&14861491(complementary)Hygr(hygromycinresistancegene):15372574(complementary)PHSVTK(HSVTKpromoter):25882835(complementary)pUCoriginofreplication:3152379mpr(ampicillinresistancegene;beta-lactamase):39434803PropagationinE.coliSuitablehoststrains:DH5aandothergeneralpurposestrains.Selectablemarker:plasmidconfersresistancetoampicillin(50g/ml)onE.colihosts.E.colireplicationorigin:pUC質(zhì)粒圖譜:

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