關(guān)于液相色譜（HPLC）回收率，請(qǐng)高手幫忙！
-
1、為什么做液相色譜的時(shí)候要計(jì)算回收率？有什么意義？2、請(qǐng)幫忙舉一個(gè)計(jì)算回收率的例子，或者發(fā)一篇介紹回收率很詳細(xì)的文獻(xiàn)到我的郵箱，請(qǐng)注明你在百度的ID，我會(huì)把懸賞分提高到50，... 1、為什么做液相色譜的時(shí)候要計(jì)算回收率？有什么意義？ 2、請(qǐng)幫忙舉一個(gè)計(jì)算回收率的例子，或者發(fā)一篇介紹回收率很詳細(xì)的文獻(xiàn)到我的郵箱，請(qǐng)注明你在百度的ID，我會(huì)把懸賞分提高到50，多謝！！ 郵箱：putinfans@163.com 展開(kāi)
全部評(píng)論(2條)
-
- 風(fēng)雨魔貓 2010-12-21 00:00:00
- 朋友，計(jì)算回收率可以知道用此方法的準(zhǔn)確性 測(cè)出的量與加入的量有個(gè)比值 建議您可以到行業(yè)內(nèi)專業(yè)的網(wǎng)站進(jìn)行交流學(xué)習(xí)！ 分析測(cè)試百科網(wǎng)樂(lè)意為你解答實(shí)驗(yàn)中碰到的各種問(wèn)題，基本上問(wèn)題都能得到解答，有問(wèn)題可去那提問(wèn)，百度上搜下就有。
-
贊(1)
回復(fù)(0)
-
- 2360818807 2010-12-20 00:00:00
- 回收率是反應(yīng)待測(cè)物在樣品分析過(guò)程中的損失的程度，損失越少，回收率越高，如果作標(biāo)液1ppm，就是1毫克/升，而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升，就是說(shuō)你的回收率是99％，這個(gè)與真實(shí)成分有密切的關(guān)系，說(shuō)明方法的準(zhǔn)確度。 意義就是回收率能反應(yīng)該方法對(duì)我們測(cè)試的項(xiàng)目損失多少，當(dāng)然回收率也有可能在以上 空白加標(biāo)回收：在沒(méi)有被測(cè)物質(zhì)的空白樣品基質(zhì)中加入定量的標(biāo)準(zhǔn)物質(zhì)，按樣品的處理步驟分析，得到的結(jié)果與理論值的比值即為空白加標(biāo)回收率。 樣品加標(biāo)回收：相同的樣品取兩分，其中一份加入定量的待測(cè)成分標(biāo)準(zhǔn)物質(zhì)；兩份同時(shí)按相同的分析步驟分析，加標(biāo)的一份所得的結(jié)果減去未加標(biāo)一份所得的結(jié)果，其差值同加入標(biāo)準(zhǔn)物質(zhì)的理論值之比即為樣品加標(biāo)回收率。 加標(biāo)回收率的測(cè)定， 是實(shí)驗(yàn)室內(nèi)經(jīng)常用以自控的一種質(zhì)量控制技術(shù). 對(duì)于它的計(jì)算方法，文獻(xiàn)中均給定了一個(gè)理論公式： 加標(biāo)回收率= (加標(biāo)試樣測(cè)定值－試樣測(cè)定值)÷加標(biāo)量×
-
贊(2)回復(fù)(0)
登錄或新用戶注冊(cè)
- 微信登錄
- 密碼登錄
- 短信登錄
請(qǐng)用手機(jī)微信掃描下方二維碼
快速登錄或注冊(cè)新賬號(hào)
微信掃碼，手機(jī)電腦聯(lián)動(dòng)
注冊(cè)登錄即表示同意《儀器網(wǎng)服務(wù)條款》和《隱私協(xié)議》
熱門(mén)問(wèn)答
- 關(guān)于液相色譜（HPLC）回收率，請(qǐng)高手幫忙！
- 1、為什么做液相色譜的時(shí)候要計(jì)算回收率？有什么意義？2、請(qǐng)幫忙舉一個(gè)計(jì)算回收率的例子，或者發(fā)一篇介紹回收率很詳細(xì)的文獻(xiàn)到我的郵箱，請(qǐng)注明你在百度的ID，我會(huì)把懸賞分提高到50，... 1、為什么做液相色譜的時(shí)候要計(jì)算回收率？有什么意義？ 2、請(qǐng)幫忙舉一個(gè)計(jì)算回收率的例子，或者發(fā)一篇介紹回收率很詳細(xì)的文獻(xiàn)到我的郵箱，請(qǐng)注明你在百度的ID，我會(huì)把懸賞分提高到50，多謝??！ 郵箱：putinfans@163.com 展開(kāi)
2010-12-19 14:22:55
858
2
- 關(guān)于微生物學(xué)的一道題，請(qǐng)高手幫忙
- “直接挑取在平板上形成的單菌落就可以獲得微生物的純培養(yǎng)”這句話是錯(cuò)的為什么？？？... “直接挑取在平板上形成的單菌落就可以獲得微生物的純培養(yǎng)” 這句話是錯(cuò)的 為什么？？？ 展開(kāi)
2010-03-15 12:16:40
730
5
- 烘箱溫度控制高手請(qǐng)幫忙?。。?！
- 有聽(tīng)說(shuō)過(guò)一種通過(guò)控制加熱功率的百分比輸出來(lái)控制烘箱溫度的方法！請(qǐng)問(wèn)是使用壽命儀表或者元件來(lái)實(shí)現(xiàn)的?。。。∏蟾呤謳兔?！或者有沒(méi)有其他方法通過(guò)控制加熱功率來(lái)實(shí)現(xiàn)烘箱的溫控??！... 有聽(tīng)說(shuō)過(guò)一種通過(guò)控制加熱功率的百分比輸出來(lái)控制烘箱溫度的方法！請(qǐng)問(wèn)是使用壽命儀表或者元件來(lái)實(shí)現(xiàn)的?。。?！求高手幫忙！ 或者有沒(méi)有其他方法通過(guò)控制加熱功率來(lái)實(shí)現(xiàn)烘箱的溫控?。。。。?！請(qǐng)告訴幫忙！ 展開(kāi)
2016-02-15 21:10:22
426
1
- 大腸桿菌檢測(cè)的問(wèn)題，請(qǐng)高手幫忙
- 如題，在大腸桿菌GB/T 4789.38 2008中，Z終的大腸桿菌數(shù)是根據(jù)LST肉湯陽(yáng)性管數(shù)查MPN表得到的，那么后面的復(fù)發(fā)酵試驗(yàn)和生化試驗(yàn)是不是可以不用做的啊? 后面是不是就是為了驗(yàn)證以上檢測(cè)的準(zhǔn)確性的呢？ 請(qǐng)高手賜教，不勝感激涕零
2010-05-10 10:10:32
607
1
- 請(qǐng)高手幫忙翻譯一下 3
- 2.2.1. Physical and physicochemical characterization The particle size distribution of the Ch-zeolite was determined using a laser diffraction equipment (CILASk 1064) and standard wet sieving (Mesh Tylerk series). Scanning electron mic... 2.2.1. Physical and physicochemical characterization The particle size distribution of the Ch-zeolite was determined using a laser diffraction equipment (CILASk 1064) and standard wet sieving (Mesh Tylerk series). Scanning electron microscopy (SEM-PHILIPSk XL20) was used for photomicrographs as well as to analyse the Ch-zeolite composition (Energy Dispersion X-ray， EDX). The sample was initially placed in a vacuum chamber for coating with a thin layer (few nanometers) of gold (Au). The specific surface area of the material was measured by the methylene blue technique and by nitrogen gas adsorption methods， with the latter also providing information about particle porosity. In the methylene blue adsorption method， aqueous solutions (50 ml) of methylene blue (100 mg l 1) were agitated using an orbital shaker (Marconik) for an hour at room temperature in the presence of different quantities of the Ch-zeolite (0.05–0.3 g). The suspensions were then allowed to settle for 23 h and the resulting supernatants were centrifuged at 5000 rpm before the analysis of the residual methylene blue concentration. Results obtained correspond to averaged values of three different experiments. The specific surface area was evaluated by the Langmuir model， assuming the formation， at high concentrations， of a dye monolayer and 1.08 nm2 molecule 1， for the cross-sectional area (Van den Hul and Lyklema， 1968). The Ch-zeolite specific surface area was evaluated by the nitrogen gas adsorption method， using automated equipment (Autosorb 1-Quantachrome Instrumentsk)， employing multipoint BET isotherm adsorption data fitting. Also from these data， the porosity of the material was evaluated through parameters such as volume of total pores (d < 206 nm)， surface area and volume of micropores (d < 2 nm; Micropore Analysis Method). Zeta potential measurements for the natural and ammonia loaded zeolite， as a function of medium pH， were determined using a Zeta Plusk equipment (Brookhaven Instruments). Suspensions (0.01% v/v) of the Ch-zeolite， previously sieved below 37 Am (400 Mesh Tylerk)， in a 10 3 mol l 1 solution of KNO3 were used and the medium pH was controlled with the addition of HNO3 (pH< 7) and KOH (pH>7)， separately. For the Ch-zeolite saturated with ammonia， suspensions of the material were prepared by the same procedure， except that the sample was loaded with 100 mg NH3–N l 1 of ammonia. 展開(kāi)
2018-11-22 17:49:22
328
0
- 分貝疊加問(wèn)題，請(qǐng)高手幫忙
- 有一種設(shè)備的噪音為3米處55分貝，如果五臺(tái)這種設(shè)備放在一起，那所產(chǎn)生的噪音是多大？求公式及原理？請(qǐng)?jiān)敿?xì)解答，會(huì)增加懸賞的，拜托了，高手們... 有一種設(shè)備的噪音為3米處55分貝，如果五臺(tái)這種設(shè)備放在一起，那所產(chǎn)生的噪音是多大？求公式及原理？請(qǐng)?jiān)敿?xì)解答，會(huì)增加懸賞的，拜托了，高手們 展開(kāi)
2013-08-31 01:54:22
1088
2
- GX液相色譜回收率怎么做
- 假設(shè)我要做A的GX液相色譜分析，如何來(lái)做A的回收率Z好不要用“加樣”之類的稱呼，希望以A來(lái)代替... 假設(shè)我要做A的GX液相色譜分析，如何來(lái)做A的回收率 Z好不要用“加樣”之類的稱呼，希望以A來(lái)代替 展開(kāi)
2012-12-10 09:40:58
678
4
- 請(qǐng)高手幫忙！我要找關(guān)于環(huán)境污染與保護(hù)的資料！
- 像什么化學(xué)污染、物理污染、核污染之類的都行。 Z好是用PPT做出來(lái)的 不過(guò)光要有材料也行！ 謝謝大家了?。?！
2006-04-13 20:49:02
376
2
- 機(jī)械制圖題目，請(qǐng)高手幫忙！不會(huì)勿擾
- 1、在圖樣中比例是指圖形與實(shí)物相應(yīng)要素的（?。┲??！ ?A)、大小之比 (B)、面積之比 (C)、線性之比 (D)、體積之比2、一張A0圖紙可以裁成（?。圓3圖紙?！ ?A)、三張 ... 1、在圖樣中比例是指圖形與實(shí)物相應(yīng)要素的（ ）之比?！ ?A)、大小之比 (B)、面積之比 (C)、線性之比 (D)、體積之比2、一張A0圖紙可以裁成（ ）張A3圖紙?！ ?A)、三張 (B)、四張 (C)、六張 (D)、八張 3、在圖樣上標(biāo)注垂直方向的尺寸時(shí)，尺寸數(shù)字應(yīng)注寫(xiě)在尺寸線的左方中間位置且字頭朝（ ）。 (A)、上 (B)、下 (C)、左 (D)、右 4、在圖樣上標(biāo)注圓弧尺寸時(shí)起止符號(hào)用（ ）。 (A)、45°斜線 (B)、箭頭 (C)、圓點(diǎn) (D)、× 5、在繪制機(jī)械圖時(shí)，通常采用的是（ ）投影法。 (A)、ZX (B)、正 (C)、斜 (D)、以上都可以 6、物體的側(cè)面投影是從物體的（ ）投影。 (A)、左面向右 (B)、右面向左 (C)、上面向下 (D)、前面向后 7、點(diǎn)的投影規(guī)律中高平齊是指（ ?。┲g 的投影關(guān)系?！ ?A)、V面和H面 (B)、V面和W面 (C)、H面和W面 8、在軸測(cè)投影圖的傾斜線上不能直接量取尺寸，只能在與軸測(cè)軸（ ）的對(duì)應(yīng)線上量取。 (A)、成一定角度的 (B)、相交 (C)、平行 (D)、不平行 9、水平線的（ ）投影反映線段的實(shí)長(zhǎng)?！ ?A)、正面 (B)、水平 (C)、側(cè)面 (D)、以上都可以 10、側(cè)垂線的（ ）投影有聚積性?！ ?A)、正面 (B)、水平 (C)、側(cè)面 (D)、以上都可以 11、平面圖形為水平面時(shí)，它的（ ?。┟嫱队胺从称矫鎴D形的實(shí)形?！ ?A)、V (B)、H (C)、W (D)、以上都可以 12、用正垂面截切圓柱時(shí)，截平面與圓柱的軸線成（ ）度角時(shí)，它的側(cè)面投影是圓?！ ?A)、30 (B)、45 (C)、60 (D)、90 13、用正垂面截切圓錐，截交線為橢圓時(shí)，橢圓短軸的正面投影在（ ）。 (A)、軸線與截交線的交點(diǎn) (B)、截交線的中點(diǎn) (C)、任意位置 (D)、截交線的端點(diǎn) 14、如果在圓球正面投影兩軸線的交點(diǎn)處有一個(gè)點(diǎn)為可見(jiàn)點(diǎn)的正面投影，它的側(cè)面投影在（ ?。??！ ?A)、兩軸線的交點(diǎn) (B)、在垂直軸線的Z高點(diǎn) (C)、在垂直軸線的Z低點(diǎn) (D)、在水平軸線的Z右點(diǎn) 15、在圓球的水平投影上有一段斜線，此線段的正面和側(cè)面投影是（ ?。?。 (A)、一個(gè)點(diǎn) (B)、一段直線 (C)、圓的一部分 (D)、橢圓的一部分 16、每張圖樣都必須畫(huà)出標(biāo)題欄，標(biāo)題欄應(yīng)位于圖紙的（ ?。?。 (A)、左上方 (B)、左下方 (C)、右上方 (D)、右下方 17、不論采用何種比例，圖形中所標(biāo)注的尺寸數(shù)值必須是實(shí)物的（ ?。┏叽纭！ ?A)、實(shí)際 (B)、圖形上量得的 (C)、放大后的 (D)、縮小后的 18、若點(diǎn)A的坐標(biāo)值為（0、20、10）時(shí)，點(diǎn)A在（ ?。┥??！ ?A)、正面投影面 (B)、水平投影面 (C)、側(cè)面投影面 (D)、X軸 --------------------------------------------------------------------------------19、在圓錐上的點(diǎn)B，它的正面和水平投影均在圓錐的垂直軸線上，側(cè)面投影在圓錐的右面輪廓線上，則點(diǎn)B一定在圓錐的（ ）素線上?！ ?A)、Z前 (B)、Z后 (C)、Z左 (D)、Z右 20、如果點(diǎn)E和點(diǎn)F在正面投影面上是重影點(diǎn)，則它們的（ ）坐標(biāo)值相同?！ ?A)、X和Y (B)、X和Z (C)、Y和Z (D)、X、Y和Z 展開(kāi)
2012-12-04 12:27:13
426
4
- 請(qǐng)高手幫忙翻譯化學(xué)名詞，急需~~~~
- 以下的技術(shù)指標(biāo)用語(yǔ)都是關(guān)于PVC顆粒的，請(qǐng)知道的幫忙準(zhǔn)確地翻譯~~~謝謝VICATPOINTHARDNESSTENSILESTRENGTHYIELDPOINTELONGATIONATBREAKFLEXURALMODULUSIMPACTSTRENGTHINFLAMMABILITYA... 以下的技術(shù)指標(biāo)用語(yǔ)都是關(guān)于PVC顆粒的，請(qǐng)知道的幫忙準(zhǔn)確地翻譯~~~謝謝 VICAT POINT HARDNESS TENSILE STRENGTH YIELD POINT ELONGATION AT BREAK FLEXURAL MODULUS IMPACT STRENGTH INFLAMMABILITY AV.A.P. IMPURITY PARTICLE NUMBER VOLATILES（INCL WATER） BULK DENSITY SIEVE RATIO “FISH EYE”NUMBER UNIT/400c㎡ RESIDUAL VCM PPM 展開(kāi)
2007-03-13 04:50:47
505
3
- 請(qǐng)高手高手高高手幫忙翻譯下面的設(shè)備名稱
- 請(qǐng)幫忙翻譯下：謝謝！斑點(diǎn)酶解系統(tǒng)自動(dòng)斑點(diǎn)切取系統(tǒng)蛋白純化系統(tǒng)基因芯片掃描系統(tǒng)二維電泳梯度膠制備系統(tǒng)全自動(dòng)電泳儀多通道懸液芯片系統(tǒng)體內(nèi)可見(jiàn)光成像系統(tǒng)如果您翻譯的十分準(zhǔn)確，我... 請(qǐng)幫忙翻譯下：謝謝！ 斑點(diǎn)酶解系統(tǒng) 自動(dòng)斑點(diǎn)切取系統(tǒng) 蛋白純化系統(tǒng) 基因芯片掃描系統(tǒng) 二維電泳梯度膠制備系統(tǒng) 全自動(dòng)電泳儀 多通道懸液芯片系統(tǒng) 體內(nèi)可見(jiàn)光成像系統(tǒng) 如果您翻譯的十分準(zhǔn)確，我將再加Z高分（好像是100分）。 我要英文，謝謝！ 展開(kāi)
2009-07-21 06:02:17
773
6
- 如何測(cè)定液相色譜的回收率
- 我查資料知道，加標(biāo)回收率=(加標(biāo)A測(cè)定值－A測(cè)定值)÷加標(biāo)量×.，想問(wèn)一下計(jì)算時(shí)用的A測(cè)定值，或者加標(biāo)A測(cè)定值，是利用液相色譜圖峰面積來(lái)計(jì)算，還是通過(guò)標(biāo)準(zhǔn)曲線計(jì)算對(duì)應(yīng)濃度？具... 我查資料知道，加標(biāo)回收率= (加標(biāo)A測(cè)定值－A測(cè)定值)÷加標(biāo)量×.，想問(wèn)一下計(jì)算時(shí)用的A測(cè)定值，或者加標(biāo)A測(cè)定值，是利用液相色譜圖峰面積來(lái)計(jì)算，還是通過(guò)標(biāo)準(zhǔn)曲線計(jì)算對(duì)應(yīng)濃度？具體用什么量計(jì)算請(qǐng)指教 展開(kāi)
2014-05-12 02:33:56
736
2
- GX液相色譜回收率如何計(jì)算
2011-11-29 07:54:54
382
5
- 高分請(qǐng)高手幫忙翻譯英文文獻(xiàn)資料（4）
- Whenliquidisheateditevaporates.Theevaporationprocessisdependentonpressure，temperatureandcompositionoftheliquidandgas.Gascanalsocondense.Inadditionthereisaconvectiveheattr... When liquid is heated it evaporates. The evaporation process is dependent on pressure， temperature and composition of the liquid and gas. Gas can also condense. In addition there is a convective heat transfer between the liquid and gas zone that must be considered. The surfaces in the gas zone also radiates from the shell to the liquid. During the blowdown process mass is usually evacuated from the gas zone， but also liquid might be released. The rate of release is dependent on density and pressure as well as the release area. As pressure and temperature change， the properties of all materials change. This has to be considered in a prediction of a blowdown process. The main purpose of a blowdown process is as earlier stated to maintain integrity of the equipment. The strength properties of the shell are the key factor on that matter. The strength is dependent on the inside pressure as well as the support forces. If the exposing forces produce stress that exceeds the ultimate tensile stress (UTS) in some regions， the integrity of the equipment is no longer maintained. In the design phase of a process plant， these aspects are crucial and must be included as a dimensional factor. For that reason prediction of the blowdown process is essential. Lately some new standards has been introduced to the industry on this matter [3] and [4]. VessFire [1] and [2] is a multi physics system designed for calculation of this kind of problems. It has been applied for some time in the oil and process industry on many projects. The system satisfies the requirements for predictions outlined in [3] and [4]. It includes all aspects described above including integrity of the shell. As part of the verification process some experiments where performed. Some of the experiments are presented here. EXPERIMENTAL STUDY The purpose of the experiments was to investigate the evaporation process and the heat transfer to the liquid and vapour. In a complex system it is important to reduce unknown parameters as far as possible. Exposure from a flame is difficult to control. Flux measurements are point values and not necessarily representative for the average exposure. In order to control the heat exposure it was decided to apply an electric heating system. The system and the verification of the system is described in [5]， [6] and [8]. The furnace was built inside a supporting tube. Figure 2 shows a general arrangement of the experimental outfit. A 0.05 mm stainless steel foil formed as a tube， 300 mm in diameter， generated the heat. The power supply was based on a 3-phase alternating current system giving 48 Volt output as maximum. The top exposure had a limit of 300 kW. The foil had a surface of about 1 m2， giving a heat flux up to 300 kW/m2. The power input could be continuously regulated from zero to maximum load. Each experiment was started from zero and brought up to the required load within a few seconds. After that the surface temperature of the heating foil was kept constant during the exposure period. Experiments with both dry objects as well as water filled object were performed. In this paper only water filled experiments are presented Figure 2 General arrangements drawing of the experimental furnace including the specimen and its support Figure 3 Illustration of the heating unit. The black part is copper conductors for the foil. The grey part is the heating foil exposing the specimen. The foil is equipped with thermo-elements all marked H， except H5 which is the temperature in a copper ring and H6 which is the temperature between the insulation and the supporting tube. 展開(kāi)
2009-03-12 04:13:30
537
6
- 請(qǐng)高手幫忙：如何查ISTP檢索號(hào)
- Exploring Sustained Competitive Advantage: The Effect of Organizational Culture on Firm Performance
2012-10-29 12:59:28
278
1
- 關(guān)于本科畢業(yè)論文選題~~~請(qǐng)大家?guī)兔~~
- 我是文化產(chǎn)業(yè)管理專業(yè)的學(xué)生，要寫(xiě)畢業(yè)論文了，老師的意見(jiàn)是要我寫(xiě)“文學(xué)網(wǎng)站或網(wǎng)絡(luò)出版運(yùn)營(yíng)方面的問(wèn)題”，因?yàn)槲覍?duì)這方面熟悉。但是這方面的文章已經(jīng)很多了，從頭到尾都說(shuō)爛了，我實(shí)... 我是文化產(chǎn)業(yè)管理專業(yè)的學(xué)生，要寫(xiě)畢業(yè)論文了，老師的意見(jiàn)是要我寫(xiě)“文學(xué)網(wǎng)站或網(wǎng)絡(luò)出版運(yùn)營(yíng)方面的問(wèn)題”，因?yàn)槲覍?duì)這方面熟悉。但是這方面的文章已經(jīng)很多了，從頭到尾都說(shuō)爛了，我實(shí)在想不出還能從哪個(gè)角度來(lái)說(shuō)它。大家能幫忙想個(gè)切入的角度嗎？ 謝謝各位了！ 展開(kāi)
2018-11-12 00:27:23
454
0
- 請(qǐng)高手回答~關(guān)于濾色片的問(wèn)題！
- 如果5600K+16分之一等于減少四檔光圈 我想問(wèn)一下5600K+4分之一等于減少幾檔光圈，或者說(shuō)控制多少暴光量？ 希望高手解答 謝謝
2006-06-15 02:39:11
671
3
4月突出貢獻(xiàn)榜
推薦主頁(yè)
最新話題
滬公網(wǎng)安備 31011502008050號(hào)
參與評(píng)論
登錄后參與評(píng)論