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  zky914 2017-05-31 00:00:00

  有，用試劑盒。 獨特的結(jié)合液/蛋白酶K迅速裂解細(xì)胞和滅活細(xì)胞內(nèi)核酸酶，然后基因組DNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜， 再通過一系列快速的漂洗－離心的步驟，YZ物去除液和漂洗液將細(xì)胞代謝物，蛋白等雜質(zhì)去除， Z后低鹽的洗脫緩沖液將純凈基因組DNA從硅基質(zhì)膜上洗脫。 特點： 1.離心吸附柱內(nèi)硅基質(zhì)膜全部采用進(jìn)口世界公司特制吸附膜，柱與柱之間吸附量差異極小，可重復(fù)性好。克服了國產(chǎn)試劑盒膜質(zhì)量不穩(wěn)定的弊端。 2.不需要使用有毒的苯酚等試劑，也不需要乙醇沉淀等步驟。 3.快速，簡捷，單個樣品操作一般可在30分鐘內(nèi)完成。 4.多次柱漂洗確保高純度，OD260/OD280典型的比值達(dá)1.7～1.9，長度可達(dá)30kb -50kb，可直接用于PCR，Southern-blot和各種酶切反應(yīng)。 發(fā)表的部分文章： 1.Vibrio zhanjiangensis sp. nov.， isolated from sea water of shrimp farming pond. Antonie van Leeuwenhoek， 2011， Published online: 29 December 2011 2.Research on resuscitation of viable but non-culturable state E.coli O157:H7. Food Science， 2012， Online Ahead of Print: Dec 11， 2012 3.Characterization of marine Pseudomonas spp. antagonist towards three tuber-rotting fungi from Jerusalem artichoke， a new industrial crop. Industrial Crops and Products， 2013， 43:556–561 4.Azospirillum humicireducens sp. nov.， a nitrogen-fixing bacterium isolated from a microbial fuel cell. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY， 2012， Published online ahead of print December 21， 2012， 5.Vibrio zhuhaiensis sp. nov.， isolated from a Japanese prawn (Marsupenaeus japonicus). Antonie van Leeuwenhoek， 2013， Published online: 22 January 2013 as DOI 10.1007/s10482-013-9878-4 6.Bacillus composti sp. nov. and Bacillus thermophilus 1 sp. nov.， two thermophilic 2 Fe(III)-reducing bacteria isolated from a compost. IJSEM， 2013， Published February 8， 2013 as DOI:10.1099/ijs.0.049106-0 7.Bacillus thermocopriae sp. nov.， 1 isolated from a compost. IJSEM， 2013，Published February 8， 2013 as doi:10.1099/ijs.0.046953-0 8.Resuscitation of Viable but Non-culturable E. coli O157:H7. 218 2013， Vol.34， No.11 9.Induction and PMA-qPCR Detection of Listeria monocytogenes in Viable but Non-Culturable State. Science paper Online 10. DETERMINATION OF DNA-DNA RELATEDNESS IN VIBRIOS BY MEANS OF REAL-TIME PCR. ACTA HYDROBIOLOGICA SINICA. Vol.37，No.4，Jul.2013 11. Kroppenstedtia guangzhouensis sp. nov.， a thermoactinomycete 1 isolated from soil.IJSEM Papers in Press. Published May 31， 2013 as doi:10.1099/ijs.0.051011-0 12. Bacillus sediminis sp. nov.， isolated from an electroactive biofilm. Antonie van Leeuwenhoek， DOI 10.1007/s10482-013-0032-0 13. Bacillus borbori sp. Nov.， Isolated From an Electrochemically Active Biofilm. Curr Microbiol (2013) 67:718–724， DOI 10.1007/s00284-013-0426-2 14. Bacillus thermotolerans sp. nov.， a thermophilic bacterium c 1 apable of reducing humus. IJSEM Papers in Press. Published April 26， 2013 as doi:10.1099/ijs.0.048942-0 15. Pseudomonas guangdongensis sp. nov.， isolated 1 from an electroactive biofilm， and amended description of the genus Pseudomonas Migula 1894. IJSEM Papers in Press. Published August 5， 2013 as doi:10.1099/ijs.0.054676-0 16. Azospirillum humicireducens sp. nov.， a nitrogen-fixing bacterium isolated from a microbial fuel cell. IJSEM Papers in Press. Published December 21， 2012 as doi:10.1099/ijs.0.046813-0 17. Resuscitation of Viable but Non-culturable E. coli O157:H7. Food science， 218 2013， Vol.34， No.11 18. Identification of lactic acid bacteria in commercial yogurt and their antibiotic resistance. Aicrobiologica Sinica 53(8):889-897;4 August 2013 19. Sinibacillus soli gen. nov.， sp. nov.， a moderately thermotolerant member of the family Bacillaceae. IJSEM Papers in Press. Published February 7， 2014 as doi:10.1099/ijs.0.055608-0. 20. Design and Properties Validation of Thiazole Orange Monoazide-A Nucleic Acid Crosslinker Based on the Activity of Esterase in Bacteria. 21. Effects of CagA+ Helicobacter pylori infection on expression of HIF-2α and ABCG2 in human gastric cancer cell line SGC7901 under normoxia and hypoxia conditions. ISSN 1009-3079 (print) ISSN 2219-2859 (online)

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