Goat anti- Rabbit Nitric oxide Collect Sample serum or blood plasma Storage: 2 - 8 °C Package size: 96 determinations PRINCIPLE OF THE METHOD The NO kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known NO concentrations and unknowns are pipetted into these wells. During the first incubation, the NO antigen and a biotinylated monoclonal antibody specific for NO are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NO present in the samples.