Abstract: Following an initial cleaning-up step on the D101 macroporous resin,a preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexaneethyl acetatemethanolwater (1:0.9:0.9:1, v/v) was used to isolate and separate schizandrin and gomisin A from Schisandra chinensis. A total of 107 mg schizandrin and 36 mg gomisin A with purities of 99.5% and 99.1% were obtained from 400 mg crude extract in one-step elution and less than 3h,and the structure identification was performed by UV, IR, MS,H-NMR and C-NMR. TBE-300A高速逆流色譜儀/制備色譜儀 TBE-1000A制備色譜儀/萃取儀/快速分離制備色譜儀 TBE300B+AKTA高速逆流色譜儀/離心分配色譜/萃取儀/制備色譜儀 TBE-200V 高速逆流色譜儀