Abstract: High-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to preparative separation and purification of verticine and verticinone from crude extracts of Bulbus Fritillariae Thunbergii by a one-step separation,using chloroformethanol0.2 mol L−1 hydrochloric acid (3:2:2, v/v/v) as a solvent system. HPLC analysis of the fractions collected on the preparative HSCCC of 200 mg of crude extracts showed that the purity of verticine (25.6 mg) was 96.8% and that of verticinone (10.3 mg) was 95.4%. The chemical identities of these components were confirmed by H-NMR and EI-MS. TBE-1000A制備色譜儀/萃取儀/快速分離制備色譜儀 TBE300B+AKTA高速逆流色譜儀/離心分配色譜/萃取儀/制備色譜儀 TBE-200V 高速逆流色譜儀