Extraction of proteins from extensively calcified osseous tissue, such as cortical bone has been particularly challenging for traditional methods of sample preparation. However, a comprehensive proteomic analysis of bone is only possible when the total protein constituency is effectively isolated. The efficiency of sample preparation is therefore a critical component of the analytical process. Historically, extraction of protein from bone required prolonged acid demineralization over several days to enable complete penetration of histochemical reagents to cellular components. Here we describe a method for the extraction of protein from ostrich tibia, which was used as a model sample to develop an extraction process that uses pressure cyc領(lǐng) technology (PCT) and also which obviates the need for acid demineralization prior to extraction. The ability to extract proteins from bone without prior demineralization offers important advantages in efficient representative extraction of protein and significant time savings during sample preparation. 利用壓力循環(huán)技術(shù)（PCT）對皮質(zhì)骨中分離出來的蛋白和多肽進(jìn)行電泳分析 從皮質(zhì)骨等充分鈣化的骨質(zhì)組織中提取蛋白的方法極大地挑戰(zhàn)了傳統(tǒng)的樣品制備方法然而這種方法只有在總的目標(biāo)蛋白有效分離的時(shí)候，廣泛的蛋白質(zhì)組學(xué)分析才能成為可能因此，樣品制備的有效性成為分析過程中的關(guān)鍵因素歷史上，從骨頭中提取蛋白需要幾天的去除礦物質(zhì)的過程使得組織化學(xué)試劑完全滲透到細(xì)胞組分中本文描述了一種從鴕鳥脛骨中提取蛋白的方法，該方法利用了壓力循環(huán)技術(shù)（PCT），而且省去了以往的在提取蛋白之前先對樣品進(jìn)行去除礦物質(zhì)的過程這種方法使得提取蛋白非常有效而且節(jié)約了大量的時(shí)間成本 SPP超高壓循環(huán)樣品制備系統(tǒng)