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Extractables and Leachables Analysis of IV Bag Systems
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2024-09-15 17:50 424閱讀次數(shù)
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Direct Thermal Extraction of the Materials and Stir Bar Sorptive Extraction of Aqueous Solutions Coupled with Thermal Desorption Gas-Chromatography with Unit Mass and High Resolution Mass Spectrometric Detection
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Extractables and Leachables Analysis of IV Bag Systems Direct Thermal Extraction of the Materials and Stir Bar Sorptive Extraction of Aqueous Solutions Coupled with Thermal Desorption Gas-Chromatography with Unit Mass and High Resolution Mass Spectrometric Detection[詳細(xì)]
2024-09-15 17:50
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R&D Systems 2017Zxin價格表 R&DSystems總公司于1976年成立于美國，在美國NASDAQ上市（TECH）。公司一直致力于各種細(xì)胞因子及其相關(guān)分子的生產(chǎn)研發(fā)。R&DSystems的產(chǎn)品覆蓋面廣，種類豐富，質(zhì)量穩(wěn)定可靠，其生產(chǎn)的各種ELISA試劑盒、重組因子及抗體以其zhuo越的品質(zhì)贏得了世界各國科研及臨床診斷機(jī)構(gòu)的青睞。2017年部分產(chǎn)品Zxin報價如下：品牌貨號品名規(guī)格價格R&D1-001-ANormalHumanIgGControl(1MG)1mg1,080R&D1002-VC-050RecombinantMouseVE-CadherinFcChimeraProtein,CF(50UG)50ug4,490R&D1003-RT-050RecombinantHumanTGF-betaRIIIsoform2FcChimera,CF(50UG)50ug4,760R&D1004-MR-050RecombinantMouseST2/IL-1R4FcChimeraProtein,CF(50UG)50ug4,490R&D1005-UN-050RecombinantHumanUNC5H3FcChimeraProtein,CF(50UG)50ug4,490R&D1006-UN-050RecombinantRatUNC5H2/UNC5BFcChimeraProtein,CF(50UG)50ug4,490R&D1007-MR-100RecombinantHumanIL-1RAPL2/IL-1R9FcChimeraProtein,CF(100UG)100ug4,490R&D1008-LR-050RecombinantMouseLymphotoxinbetaR/TNFRSF3FcChimera,CF(50UG)50ug4,490R&D1008-LY-010RecombinantMouseLymphotoxinalpha2/beta1Protein(10UG)10ug4,490R&D1008-LY-010/CFRecombinantMouseLymphotoxinalpha2/beta1Protein,CF(10UG)10ug4,490R&D1009-CL-010RecombinantCottonRatIL-1beta/IL-1F2Protein(10UG)10ug3,540R&D1009-CL-010/CFRecombinantCottonRatIL-1beta/IL-1F2Protein,CF(10UG)10ug3,540R&D100-B-001HumanTGF-beta1Protein(1UG)1ug3,940R&D100-B-001/CFHumanTGF-beta1Protein,CF(1UG)1ug3,940R&D100-B-010HumanTGF-beta1Protein(10UG)10ug23,600R&D100-B-010/CFHumanTGF-beta1Protein,CF(10UG)10ug23,600R&D1010-CR-025RecombinantCottonRatCCL5/RANTESProtein(25UG)25ug3,810R&D1010-CR-025/CFRecombinantCottonRatCCL5/RANTESProtein,CF(25UG)25ug3,810R&D1011-CR-025RecombinantCottonRatTNF-alphaProtein(25UG)25ug2,850R&D1011-CR-025/CFRecombinantCottonRatTNF-alphaProtein,CF(25UG)25ug2,850R&D1012-PS-010RecombinantPro-TNF-alphaFusionProteinProtein,CF(10UG)10ug4,490R&D1013-CY-010RecombinantMouseCathepsinHProtein,CF(10UG)10ug4,490R&D1014-AS-010RecombinantHumanCathepsinDProtein,CF(10UG)10ug4,630R&D1015-P2-020RecombinantPorcineIL-2(Cys146Ala)Protein(20UG)20ug4,490R&D1015-P2-020/CFRecombinantPorcineIL-2(Cys146Ala)Protein,CF(20UG)20ug4,490R&D1017-MP-025RecombinantMouseCCL24/Eotaxin-2/MPIF-2(aa27-119)Protein(25UG)25ug3,130R&D1017-MP-025/CFRecombinantMouseCCL24/Eotaxin-2/MPIF-2(aa27-119),CF(25UG)25ug3,130R&D1019-B7-100RecombinantMouseB7-H1FcChimeraProtein,CF(100UG)100ug4,490R&D101-B1-001PorcineTGF-beta1Protein(1UG)1ug3,540R&D101-B1-001/CFPorcineTGF-beta1Protein,CF(1UG)1ug3,540R&D101-B1-010PorcineTGF-beta1Protein(10UG)10ug21,690R&D101-B1-010/CFPorcineTGF-beta1Protein,CF(10UG)10ug21,690R&D1020-RL-050RecombinantHumanCD25/IL-2RalphaFcChimeraProtein,CF(50UG)50ug4,490R&D1021-PD-100RecombinantMousePD-1FcChimeraProtein,CF(100UG)100ug4,490R&D1022-PL-100RecombinantMousePD-L2/B7-DCFcChimeraProtein,CF(100UG)100ug4,630R&D1023-ML-010RecombinantMouseIL-10(Cys167Tyr)Protein(10UG)10ug3,940R&D1023-ML-010/CFRecombinantMouseIL-10(Cys167Tyr)Protein,CF(10UG)10ug3,940R&D1024-MB-025RecombinantCottonRatCCL4/MIP-1betaProtein(25UG)25ug4,490R&D1024-MB-025/CFRecombinantCottonRatCCL4/MIP-1betaProtein,CF(25UG)25ug4,490R&D1025-CE-025RecombinantMouseBetacellulinProtein(25UG)25ug4,350R&D1025-CE-025/CFRecombinantMouseBetacellulinProtein,CF(25UG)25ug4,350R&D1026-GR-100RecombinantMouseIFN-gammaR1FcChimeraProtein,CF(100UG)100ug4,760R&D1027-B3-100RecombinantHumanB7-H3FcChimeraProtein,CF(100UG)100ug4,630R&D1028-CL-050RecombinantHumanCD30Ligand/TNFSF8Protein,CF(50UG)50ug3,130R&D1029-AS-010RecombinantMouseCathepsinDProtein,CF(10UG)10ug4,490R&D102-B2-001PorcineTGF-beta2Protein(1UG)1ug5,040R&D102-B2-001/CFPorcineTGF-beta2Protein,CF(1UG)1ug5,040R&D1030-FN-01MBovineFibronectinProtein,CF(1MG)1mg1,900R&D1030-FN-05MBovineFibronectinProtein,CF(5MG)5mg4,490R&D1031-AD-020RecombinantHumanADAM8Protein,CF(20UG)20ug5,040R&D1033-CY-010RecombinantMouseCathepsinX/Z/PProtein,CF(10UG)10ug4,490R&D1034-CY-010RecombinantMouseCathepsinC/DPPIProtein,CF(10UG)10ug4,490R&D1035-IL-025RecombinantHumanIL-19Protein(25UG)25ug4,570R&D1035-IL-025/CFRecombinantHumanIL-19Protein,CF(25UG)25ug4,570R&D1039-2B-050RecombinantHuman2B4/CD244/SLAMF4FcChimeraProtein,CF(50UG)50ug4,630R&D1040-BR-050RecombinantMouseIL-17RBFcChimeraProtein,CF(50UG)50ug4,490R&D1041-TC-050RecombinantMouseTACI/TNFRSF13BFcChimeraProtein,CF(50UG)50ug4,490R&D1042-PR-100RecombinantMousePDGFRbetaFcChimeraProtein,CF(100UG)100ug4,760R&D1048-PI-010RecombinantHumanHAI-1Protein,CF(10UG)10ug4,490R&D1049-SE-010RecombinantHumanCathepsinA/LysosomCarboxypeptidaseA,CF(10UG)10ug4,630R&D1051-CA-050RecombinantChickenCaronteFcChimeraProtein,CF(50UG)50ug4,490R&D1052-ER-050RecombinantHumanEN-RAGEProtein,CF(50UG)50ug4,490R&D1054-OX-010RecombinantHumanOX40Ligand/TNFSF4Protein,CF(10UG)10ug4,490R&D1055-AA-050RecombinantRatPDGF-AAProtein,CF(50UG)50ug3,670R&D1056-TK-050RecombinantRatTrkAFcChimeraProtein,CF(50UG)50ug4,490R&D1057-TK-050RecombinantRatNotch-1FcChimeraProtein,CF(50UG)50ug4,490R&D1060-DE-100RecombinantMouseDecorinProtein,CF(100UG)100ug3,940R&D1061-RM-025RecombinantCottonRatCCL3/MIP-1alphaProtein(25UG)25ug4,490R&D1061-RM-025/CFRecombinantCottonRatCCL3/MIP-1alphaProtein,CF(25UG)25ug4,490R&D1062-PR-050RecombinantMousePDGFRalphaFcChimeraProtein,CF(50UG)50ug4,900R&D1063-SE-010RecombinantHumanCoagulationFactorXaProtein,CF(10UG)10ug4,490R&D1064-IL-010RecombinantHumanIL-10(aa19-178)Protein(10UG)10ug3,940R&D1064-IL-010/CFRecombinantHumanIL-10(aa19-178)Protein,CF(10UG)10ug3,940R&D1065-AP-050RecombinantHumanAdiponectin/Acrp30Protein,CF(50UG)50ug4,350R&D1066-AB-005RecombinantHumanActivinABProtein(5UG)5ug4,760R&D1066-AB-005/CFRecombinantHumanActivinABProtein,CF(5UG)5ug4,760R&D1067-GH-025RecombinantHumanGrowthHormone(GH)Protein(25UG)25ug2,580R&D1067-GH-025/CFRecombinantHumanGrowthHormone(GH)Protein,CF(25UG)25ug2,580R&D1068-EP-050RecombinantMouseEpiregulinProtein(50UG)50ug4,490R&D1068-EP-050/CFRecombinantMouseEpiregulinProtein,CF(50UG)50ug4,490R&D1069-RN-050RecombinantMouseResistinProtein(50UG)50ug3,130R&D1069-RN-050/CFRecombinantMouseResistinProtein,CF(50UG)50ug3,130R&D1070-RM-025RecombinantRhesusMacaqueTNF-alphaProtein(25UG)25ug2,850R&D1070-RM-025/CFRecombinantRhesusMacaqueTNF-alphaProtein,CF(25UG)25ug2,850R&D1071-CY-010RecombinantHumanCathepsinC/DPPIProtein,CF(10UG)10ug4,490R&D1072-SL-050RecombinantHumanSiglec-5FcChimeraProtein,CF(50UG)50ug4,490R&D1073-BP-010RecombinantHumanBMP-8aProtein(10UG)10ug3,940R&D1073-BP-010/CFRecombinantHumanBMP-8aProtein,CF(10UG)10ug3,940R&D1073-BPC-025RecombinantHumanBMP-8a(CHO-expressed)Protein,CF(25UG)25ug4,490R&D1074-VJ-050RecombinantHumanJAM-B/VE-JAMFcChimeraProtein,CF(50UG)50ug4,490R&D1077-JM-050RecombinantMouseJAM-AFcChimeraProtein,CF(50UG)50ug4,490R&D1078-IL-025RecombinantHumanIL-36alpha/IL-1F6Protein(25UG)25ug4,490R&D1078-IL-025/CFRecombinantHumanIL-36alpha/IL-1F6Protein,CF(25UG)25ug4,490R&D1079-NE-050RecombinantMouseNeogeninFcChimeraProtein,CF(50UG)50ug4,490R&D1080-CY-010RecombinantHumanCathepsinVProtein,CF(10UG)10ug4,490R&D1081-IL-020RecombinantHumanIL-2(Cys145Ser)Protein(20UG)20ug2,990R&D1081-IL-020/CFRecombinantHumanIL-2(Cys145Ser)Protein,CF(20UG)20ug2,990R&D1082-CR-025RecombinantCottonRatIFN-alpha(Cys109Tyr)Protein(25UG)25ug3,940R&D1082-CR-025/CFRecombinantCottonRatIFN-alpha(Cys109Tyr)Protein,CF(25UG)25ug3,940R&D1083-AB-050RecombinantMouseIFN-alpha/betaR2FcChimeraProtein,CF(50UG)50ug4,490R&D1084-M2-025RecombinantCottonRatMIP-2Protein(25UG)25ug4,490R&D1084-M2-025/CFRecombinantCottonRatMIP-2Protein,CF(25UG)25ug4,490R&D1085-AR-025RecombinantMouseArteminProtein(25UG)25ug4,490R&D1085-AR-025/CFRecombinantMouseArteminProtein,CF(25UG)25ug4,490R&D1086-PD-050RecombinantHumanPD-1FcChimeraProtein,CF(50UG)50ug4,630R&D1087-BP-025RecombinantHumanIL-22BPFcChimeraProtein,CF(25UG)25ug4,490R&D1088-B2-200RecombinantZebrafishEphrin-B2FcChimeraProtein,CF(200UG)200ug4,490R&D1089-R2-025RecombinantHumanTNFRII/TNFRSF1B(aa24-206)Protein(25UG)25ug4,350R&D1089-R2-025/CFRecombinantHumanTNFRII/TNFRSF1B(aa24-206)Protein,CF(25UG)25ug4,350R&D1090-TW-025RecombinantHumanTWEAK/TNFSF12Protein(25UG)25ug4,490R&D1090-TW-025/CFRecombinantHumanTWEAK/TNFSF12Protein,CF(25UG)25ug4,490R&D1091-CK-025RecombinantMouseCXCL7/ThymusChemokine-1(aa48-109)(25UG)25ug4,490R&D1091-CK-025/CFRecombinantMouseCXCL7/ThymusChemokine-1(aa48-109),CF(25UG)25ug4,490R&D1093-XD-050RecombinantHumanEDA2R/TNFRSF27/XEDARFcChimera,CF(50UG)50ug4,490R&D1095-ER-002RecombinantHumanEGFR/ErbB1Protein(2UG)2ug1,490R&D1097-EN-025RecombinantHumanEndoglin/CD105Protein(25UG)25ug4,490R&D1097-EN-025/CFRecombinantHumanEndoglin/CD105Protein,CF(25UG)25ug4,490R&D1098-ES-050RecombinantHumanEndostatinProtein,CF(50UG)50ug4,350R&D1099-IL-025RecombinantHumanIL-36beta/IL-1F8Protein(25UG)25ug4,350R&D1099-IL-025/CFRecombinantHumanIL-36beta/IL-1F8Protein,CF(25UG)25ug4,350R&D109-OP-050BovineOsteopontin(OPN)Protein(50UG)50ug3,260R&D109-OP-050/CFBovineOsteopontin(OPN)Protein,CF(50UG)50ug3,260R&D11002-1RecombinantHumanIFN-alphaProteinSampler(12x100,000U)(1KT)1ea30,750R&D1102-IL-025RecombinantHumanIL-20Protein(25UG)25ug4,570R&D1102-IL-025/CFRecombinantHumanIL-20Protein,CF(25UG)25ug4,570R&D1103-JM-050RecombinantHumanJAM-AFcChimeraProtein,CF(50UG)50ug4,490R&D1106-PI-010RecombinantHumanHAI-2AProtein,CF(10UG)10ug4,490R&D1107-PI-010RecombinantMouseHAI-2BProtein,CF(10UG)10ug4,490R&D1108-SE-010RecombinantHumanKallikrein5Protein,CF(10UG)10ug4,490R&D1109-N1-025RecombinantMouseNetrin-1Protein(25UG)25ug4,490R&D1109-N1-025/CFRecombinantMouseNetrin-1Protein,CF(25UG)25ug4,490R&D110-HG-100RecombinantHumanIgG1Fc,CF(100UG)100ug4,490R&D11100-1RecombinantHumanIFN-alphaA(alpha2a)Protein(5MU)5MU3,560R&D11101-1RecombinantHumanIFN-alphaAProtein,CF(5UG)5ug3,270R&D11101-2RecombinantHumanIFN-alphaAProtein,CF(20UG)20ug4,790R&D11105-1RecombinantHumanIFN-alpha2(alpha2b)Protein(100000UN)100000un3,420R&D11115-1RecombinantHumanIFN-alphaB2(alpha8)Protein(100000UN)100000un4,360R&D11120-1RecombinantHumanIFN-alphaC(alpha10)Protein(100000UN)100000un3,990R&D11125-1RecombinantHumanIFN-alphaD(alpha1(Val114))Protein(100000UN)100000un3,990R&D1112-PR-050RecombinantRatProlactinRFcChimeraProtein,CF(50UG)50ug4,490R&D11130-1RecombinantHumanIFN-alphaF(alpha21)Protein(100000UN)100000un3,990R&D11135-1RecombinantHumanIFN-alphaG(alpha5)Protein(100000UN)100000un3,990R&D11145-1RecombinantHumanIFN-alphaH2(alpha14)Protein(100000UN)100000un3,990R&D1114-GR-025RecombinantCottonRatGROProtein(25UG)25ug4,490R&D1114-GR-025/CFRecombinantCottonRatGROProtein,CF(25UG)25ug4,490R&D11150-1RecombinantHumanIFN-alphaI(alpha17)Protein(100000UN)100000un3,990R&D1115-AB-010RecombinantRatPDGF-ABProtein,CF(10UG)10ug3,540R&D11160-1RecombinantHumanIFN-alphaJ1(alpha7)Protein(100000UN)100000un3,990R&D11165-1RecombinantHumanIFN-alphaK(alpha6)Protein(100000UN)100000un3,990R&D1116-CK-025RecombinantRatCXCL7/ThymusChemokine-1Protein(25UG)25ug4,490R&D1116-CK-025/CFRecombinantRatCXCL7/ThymusChemokine-1Protein,CF(25UG)25ug4,490R&D11175-1RecombinantHumanIFN-alpha1(AlphaD(Ala114))Protein(100000UN)100000un3,990R&D11177-1RecombinantHumanIFN-alpha4a(alphaM1)Protein(100000UN)100000un3,990R&D1117-IP-025RecombinantCottonRatCXCL10/IP-10Protein,CF(25UG)25ug4,490R&D11180-1RecombinantHumanIFN-alpha4b(alpha4)Protein(100000UN)100000un3,990R&D1118-DK-050RecombinantHumanDkk-3Protein,CF(50UG)50ug4,490R&D11190-1RecombinantHumanIFN-alphaWA(alpha16)Protein(100000UN)100000un3,990R&D1119-AC-025RecombinantMousegAdiponectin/gAcrp30Protein,CF(25UG)25ug4,490R&D111-BM-010RecombinantZebrafishBMP-2aProtein(10UG)10ug4,490R&D111-BM-010/CFRecombinantZebrafishBMP-2aProtein,CF(10UG)10ug4,490R&D11200-1UniversalTypeIIFNProtein(100000UN)100000un5,080R&D11200-2UniversalTypeIIFNProtein(1MU)1MU8,790R&D1120-FZ-050RecombinantMouseFrizzled-1FcChimeraProtein,CF(50UG)50ug4,490R&D1121-TL-010RecombinantMouseTRAIL/TNFSF10Protein(10UG)10ug4,350R&D1121-TL-010/CFRecombinantMouseTRAIL/TNFSF10Protein,CF(10UG)10ug4,350R&D1126-ZN-010RecombinantMouseNeprilysinProtein,CF(10UG)10ug4,490R&D1126-ZNC-010RecombinantMouseNeprilysin(CHO-expressed)Protein,CF(10UG)10ug4,490R&D1127-EP-025RecombinantMouseEpigenProtein(25UG)25ug4,490R&D1127-EP-025/CFRecombinantMouseEpigenProtein,CF(25UG)25ug4,490R&D1128-BM-010RecombinantZebrafishBMP-4Protein(10UG)10ug4,490我們公司Zda優(yōu)勢是強大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時限加急費全免4：價格公道，絕大部分價格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都是價格Zdi,因為價格Zdi意味著沒有服務(wù).5：良好的信譽，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們還是Santa,AdvancedBiotechnologiesInc,SpringBioscience,bangs,BBInternational,crystalchem,dianova,FDNeurotechnologies,Inc.FormuMaxScientific,Inc,GePromegaridege,GlycotopeBiotechnologyGmbH;iduron,InnovativeResearchofAmerica,Ludger,neuroprobe,SpringBioscience,Polysciences,prospecbi,QA-BIO,quickzyme,FULLERLABS,INC,sterlitech；sysy,TriLinkBioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。7：我們還是invitrogen,qiagen,SpringBioscienceam,sigma;Promega,roche,merck,rnd,BD,GE,pierce,BioLegend等知名批發(fā)，歡迎合作。[詳細(xì)]
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2021-02-23 10:44
產(chǎn)品樣冊
大鼠IV型膠原（Collagen IV）ELISA試劑盒說明書 電話：021-6533363955229872網(wǎng)址：http://www.westang.com大鼠IV型膠原（CollagenIV）ELISA試劑盒（用于血清、血漿、細(xì)胞培養(yǎng)上清液和其它生物體液內(nèi)）原理本實驗采用雙抗體夾心ABC-ELISA法。用抗大鼠CollagenIV單抗包被于酶標(biāo)板上，標(biāo)準(zhǔn)品和樣品中的CollagenIV與單抗結(jié)合，加入生物素化的抗大鼠CollagenIV，形成免疫復(fù)合物連接在板上，辣根過氧化物酶標(biāo)記的Streptavidin與生物素結(jié)合，加入底物工作液顯藍(lán)色，Z后加終止液硫酸，在450nm處測OD值，CollagenIV濃度與OD值成正比，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中CollagenIV濃度。試劑盒組成（2-8℃保存）酶標(biāo)板（CoatedWells）96孔酶標(biāo)抗體工作液（EnzymeConjugate）12ml10×標(biāo)本稀釋液（SampleBuffer）12ml20×濃縮洗滌液（WashBuffer）50ml標(biāo)準(zhǔn)品（Standards）：2000ng/瓶2瓶底物工作液（TMBSolution）12ml**抗體工作液（BiotinylatedAntibody）12ml終止液（StopSolution）12ml準(zhǔn)備試劑與收集血樣1.收集標(biāo)本：血清、血漿（EDTA、檸檬酸鹽、肝素抗凝）、細(xì)胞培養(yǎng)上清液、組織勻漿等盡早檢測，2-8℃保存48小時；更長時間須冷凍（-20℃或-70℃）保存，避免反復(fù)凍融。2.標(biāo)準(zhǔn)品液配制：使用前加入1ml蒸餾水混勻，配成2000ng/ml的溶液。設(shè)標(biāo)準(zhǔn)管8管，每管加入標(biāo)本稀釋液300ul。在**管中加入2000ng/ml的標(biāo)準(zhǔn)品溶液300ul混勻后用加樣器吸出300ul，移至第二管。如此反復(fù)作對倍稀釋，從第七管中吸出300ul棄去。第八管為空白對照。3.10×標(biāo)本稀釋液用蒸餾水作1:10倍稀釋（示例：1ml濃稀釋液+9ml蒸餾水）。4.洗滌液：用重蒸水1:20稀釋（示例：1ml濃縮洗滌液加入19ml的重蒸水）檢測程序1.加樣：每孔各加入標(biāo)準(zhǔn)品或待測樣品100ul，將反應(yīng)板充分混勻后置37℃120分鐘。2.洗板：用洗滌液將反應(yīng)板充分洗滌4-6次，向濾紙上印干。3.每孔中加入**抗體工作液100ul。將反應(yīng)板充分混勻后置37℃60分鐘。4.洗板：同前。5.每孔加酶標(biāo)抗體工作液100ul。將反應(yīng)板置37℃30分鐘。6.洗板：同前。7.每孔加入底物工作液100ul，置37℃暗處反應(yīng)15分鐘。8.每孔加入100ul終止液混勻。9.30分鐘內(nèi)用酶標(biāo)儀在450nm處測吸光值。結(jié)果計算與判斷1.所有OD值都應(yīng)減除空白值后再行計算。2.以標(biāo)準(zhǔn)品1000、500、250、125、62.5、31.2、15.6、0ng/ml為橫坐標(biāo)，OD值為縱坐標(biāo)，在坐標(biāo)紙上作圖，畫出標(biāo)準(zhǔn)曲線。3.根據(jù)樣品OD值在該曲線圖上查出相應(yīng)CollagenIV含量。試劑盒性能1.靈敏度：Z小的CollagenIV檢測濃度小于10ng/ml。2.特異性：可同時檢測重組或天然的大鼠CollagenIV。不與大鼠其它因子有交叉反應(yīng)。3.重復(fù)性：板內(nèi)、板見變異系數(shù)均小于12％。注意事項1.以上標(biāo)準(zhǔn)孔及待測樣品均建議做復(fù)孔，每次測定應(yīng)同時做標(biāo)準(zhǔn)曲線。2.洗滌過程很關(guān)鍵。洗滌不充分將導(dǎo)致極ng確度誤差及OD值錯誤地升高。3.板條開封后剩余板條要再封好，保持板條干燥。4.本試劑盒宜置4oC冰箱保存。5.本試劑盒僅用于科研，不能用于臨床診斷！[詳細(xì)]
2018-09-13 10:00
產(chǎn)品樣冊
小鼠IV型膠原（Collagen IV）ELISA試劑盒說明書 小鼠IV型膠原（CollagenIV）ELISA試劑盒（用于血清、血漿、細(xì)胞培養(yǎng)上清液和其它生物體液內(nèi)）原理本實驗采用雙抗體夾心ABC-ELISA法。用抗小鼠CollagenIV單抗包被于酶標(biāo)板上，標(biāo)準(zhǔn)品和樣品中的CollagenIV與單抗結(jié)合，加入生物素化的抗小鼠CollagenIV，形成免疫復(fù)合物連接在板上，辣根過氧化物酶標(biāo)記的Streptavidin與生物素結(jié)合，加入底物工作液顯藍(lán)色，Z后加終止液硫，在450nm處測OD值，CollagenIV濃度與OD值成正比，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中CollagenIV濃度。試劑盒組成（2-8℃保存）酶標(biāo)板（CoatedWells）96孔酶標(biāo)抗體工作液（EnzymeConjugate）12ml10×標(biāo)本稀釋液（SampleBuffer）12ml20×濃縮洗滌液（WashBuffer）50ml標(biāo)準(zhǔn)品（Standards）：2000ng/瓶2瓶底物工作液（TMBSolution）12ml**抗體工作液（BiotinylatedAntibody）12ml終止液（StopSolution）12ml準(zhǔn)備試劑與收集血樣1.收集標(biāo)本：血清、血漿（EDTA、檸檬酸鹽、肝素抗凝）、細(xì)胞培養(yǎng)上清液、組織勻漿等盡早檢測，2-8℃保存48小時；更長時間須冷凍（-20℃或-70℃）保存，避免反復(fù)凍融。2.標(biāo)準(zhǔn)品液配制：使用前加入1ml蒸餾水混勻，配成2000ng/ml的溶液。設(shè)標(biāo)準(zhǔn)管8管，每管加入標(biāo)本稀釋液300ul。在**管中加入2000ng/ml的標(biāo)準(zhǔn)品溶液300ul混勻后用加樣器吸出300ul，移至第二管。如此反復(fù)作對倍稀釋，從第七管中吸出300ul棄去。第八管為空白對照。3.10×標(biāo)本稀釋液用蒸餾水作1:10倍稀釋（示例：1ml濃稀釋液+9ml蒸餾水）。4.洗滌液：用重蒸水1:20稀釋（示例：1ml濃縮洗滌液加入19ml的重蒸水）檢測程序1.加樣：每孔各加入標(biāo)準(zhǔn)品或待測樣品100ul，將反應(yīng)板充分混勻后置37℃120分鐘。2.洗板：用洗滌液將反應(yīng)板充分洗滌4-6次，向濾紙上印干。3.每孔中加入**抗體工作液100ul。將反應(yīng)板充分混勻后置37℃60分鐘。4.洗板：同前。5.每孔加酶標(biāo)抗體工作液100ul。將反應(yīng)板置37℃30分鐘。6.洗板：同前。7.每孔加入底物工作液100ul，置37℃暗處反應(yīng)15分鐘。8.每孔加入100ul終止液混勻。9.30分鐘內(nèi)用酶標(biāo)儀在450nm處測吸光值。結(jié)果計算與判斷1.所有OD值都應(yīng)減除空白值后再行計算。2.以標(biāo)準(zhǔn)品1000、500、250、125、62.5、31.2、15.6、0ng/ml為橫坐標(biāo)，OD值為縱坐標(biāo)，在坐標(biāo)紙上作圖，畫出標(biāo)準(zhǔn)曲線。3.根據(jù)樣品OD值在該曲線圖上查出相應(yīng)CollagenIV含量。試劑盒性能1.靈敏度：Z小的CollagenIV檢測濃度小于10ng/ml。2.特異性：可同時檢測重組或天然的小鼠CollagenIV。不與小鼠其它因子有交叉反應(yīng)。3.重復(fù)性：板內(nèi)、板見變異系數(shù)均小于12％。注意事項1.以上標(biāo)準(zhǔn)孔及待測樣品均建議做復(fù)孔，每次測定應(yīng)同時做標(biāo)準(zhǔn)曲線。2.洗滌過程很關(guān)鍵。洗滌不充分將導(dǎo)致極ng確度誤差及OD值錯誤地升高。3.板條開封后剩余板條要再封好，保持板條干燥。4.本試劑盒宜置4oC冰箱保存。5.本試劑盒僅用于科研，不能用于臨床診斷！[詳細(xì)]
2018-09-13 10:01
產(chǎn)品樣冊
大鼠 IV型膠原 （Co IV） ELISA 檢測試劑盒 試驗原理：CoIV試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知CoIV濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將CoIV和生物素標(biāo)記的抗體同時溫育。洗滌后，加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌，去除未結(jié)合的酶結(jié)合物，然后加入底物A、B，和酶結(jié)合物同時作用。產(chǎn)生顏色。顏色的深淺和樣品中CoIV的濃度呈比例關(guān)系。試劑盒內(nèi)容及其配制試劑盒成份（2-8℃保存）96孔配置48孔配置配制96/48人份酶標(biāo)板1塊板（96T）半塊板（48T）即用型塑料膜板蓋1塊半塊即用型標(biāo)準(zhǔn)品：800ng/ml1瓶（0.6ml）1瓶（0.3ml）按說明書進(jìn)行稀稀空白對照1瓶（1.0ml）1瓶（0.5ml）即用型標(biāo)準(zhǔn)品稀釋緩沖液1瓶（5ml）1瓶（2.5ml）即用型生物素標(biāo)記的抗CoIV抗體1瓶（6ml）1瓶（3.0ml）即用型親和鏈酶素-HRP1瓶（10ml）1瓶（5.0ml）即用型洗滌緩沖液1瓶（20ml）1瓶（10ml）按說明書進(jìn)行稀釋底物A1瓶（6.0ml）1瓶（3.0ml）即用型底物B1瓶（6.0ml）1瓶（3.0ml）即用型終止液1瓶（6.0ml）1瓶（3.0ml）即用型標(biāo)本稀釋液1瓶（12ml）1瓶（6.0ml）即用型自備材料蒸餾水。加樣器：5ul、10ul、50ul、100ul、200ul、500ul、1000ul。振蕩器及磁力攪拌器等。安全性避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。實驗中不要吃喝、抽煙或使用化妝品。不要用嘴吸取試劑盒里的任何成份。[詳細(xì)]
2018-09-19 10:01
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專利
Cell Guidance Systems Zxin價格表 熱烈祝賀上海起發(fā)實驗試劑有限公司與AthensResearch&Technology公司達(dá)成代理合作協(xié)議，起發(fā)實驗試劑也會繼續(xù)秉承客戶至上的原則，為您的實驗研究提供更多優(yōu)質(zhì)產(chǎn)品與服務(wù)。AthensResearch&Technology公司位于美國喬治亞州雅典市，從1986年以來一直致力于純化高純度，高活性的人類蛋白質(zhì)和開發(fā)這些蛋白的多克隆抗血清。我們的常規(guī)產(chǎn)品包括絲氨酸蛋白酶、蛋白酶YZ劑、中性粒細(xì)胞酶、載脂蛋白、脂蛋白，血小板蛋白、轉(zhuǎn)鐵蛋白、免疫球蛋白等。二十年多年來，世界各地的研究人員都在使用我們公司的產(chǎn)品。在炎癥、冠狀動脈疾病、自身免疫性疾病、癌癥、老年癡呆癥等等研究的主要科學(xué)出版物中都能看到引用我們公司的產(chǎn)品。我們提供優(yōu)質(zhì)，可靠的產(chǎn)品和出色的客戶服務(wù)。我們期待著您的業(yè)務(wù)。如果需要定制研究服務(wù)，AthensResearch&Technology公司能夠根據(jù)您的項目要求提供無與倫比的試劑。產(chǎn)品特色:多種天然提取的人類蛋白質(zhì)產(chǎn)品；多種兔抗人類蛋白質(zhì)抗血清。AthensResearch&Technology是成立于2010年的新興生物高科技制品公司，該公司擁有BSL-2實驗室及超過11000平方英尺的cGMP生產(chǎn)廠房，并且通過ISO9001：2008認(rèn)證。Athens公司致力于純化分離高純度，高活性的人類蛋白質(zhì)及研發(fā)多克隆抗血清產(chǎn)品。提供包括高純度及活性的絲氨酸蛋白酶，蛋白酶YZ劑，中性粒細(xì)胞酶，載脂蛋白，脂蛋白，血小板蛋白，轉(zhuǎn)鐵蛋白，免疫球蛋白等等。公司產(chǎn)品適用于炎癥，冠狀動脈疾病，自身免疫性疾病，癌癥，阿爾茨海默氏病等眾多研究領(lǐng)域，已被世界ding級制藥公司及診斷試劑公司用于體外診斷試劑盒/免疫檢測試劑盒、藥物篩選、細(xì)胞培養(yǎng)液（包括干細(xì)胞）等產(chǎn)品研發(fā)。除了人類蛋白質(zhì)，我們還從動物血清和組織中分離多種蛋白質(zhì)及酶類。此外，AthensResearchandTechnology還提供特殊試劑/蛋白定制服務(wù)，以滿足研究人員的不同需求。ImmunoglobulinM(IgM),HumanMyelomaPlasmaPRODUCTNUMBER:16-16-090713-MMW:950,000ExtinctionCoefficient:1.18Frozenin50mMTris-HCl,pH8.0,with200mMNaCland0.05%NaN3.Storage:-20°CBuyPurifiedNativeHumanImmunoglobulinM(IgM),HumanMyelomaPlasma.Price$35.00/mg.BulkQtyAvailable.IgMisfoundinnormalplasmaataconcentrationof1.2to4.0mg/ml.Itisthefirstimmunoglobulinproducedduringtheimmuneresponse,thefirstantibodyinneonates,andisinvolvedinpathogenesisofautoimmunediseases.AdiminishedlevelofIgMisassociatedwithWiskott-Aldrichsyndrome.Purity:>=95%bySDS-PAGEPreparedfrommyelomaplasmashowntobenonreactiveforHBsAg,anti-HCV,anti-HBc,andnegativeforanti-HIV1&2byFDAapprovedtests.AthensResearch&Technologyproductsarelaboratoryreagentsandarenottobeadministeredtohumansorusedforanydrugpurpose.Forresearchorfurthermanufacturinguseonly.AthensResearch16-16-090713-M大量現(xiàn)貨我們公司Zda優(yōu)勢是強大的采購，1：基本什么都能進(jìn)口，血清，抗體，耗材，還有部分限制進(jìn)口的，2：貨品全，現(xiàn)經(jīng)營過700多個品牌，基本所有生物試劑耗材都可以進(jìn)口，特別是冷偏的產(chǎn)品那就更有優(yōu)勢，3：提供加急服務(wù)，一般1-2周到貨，超過時限加急費全免4：價格公道，絕大部分價格有優(yōu)勢，當(dāng)然不能保證1**%產(chǎn)品都是價格Zdi,因為價格Zdi意味著沒有服務(wù).5：良好的信譽，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司6：我們還是santa,AdvancedBiotechnologiesInc;AthensResearch&Technology,bangs,BBInternational,crystalchem,dianova,AthensResearch&Technology,Inc.Genebridege;AthensResearch&TechnologyBiotechnologyGmbH;iduron;AthensResearch&Technology；AthensResearch&Technology；neuroprobe;omicronbio;Polysciences;prospecbi;QA-BIO；quickzyme；RESEARCHDIETS,INC；sterlitech；sysy；TriLinkBioTechnologies,Inc；worthington-biochem;zyagen;......幾十家國外公司授權(quán)代理7：我們還從事invitrogen，qiagen;abcam;sigma;neb;roche;merck;rnd;BD;GE;pierce;BioLegend....等批發(fā)[詳細(xì)]
2018-09-29 10:02
產(chǎn)品樣冊
Mamac Systems壓力傳感器資料大全 MamacSystems壓力傳感器資料大全MamacSystems傳感器美國原裝我司優(yōu)勢：1）在美國和德國有公司，直接從廠家采購，一手價格，保證所有產(chǎn)品均為原裝。2）價格合理，繞過層層代理，Zda限度的讓利給客戶。3）渠道廣泛，國內(nèi)有代理，或者有客戶保護(hù)廠家不賣的產(chǎn)品，只要您能提供型號，我們同樣可以從各國的分銷商來采購。4）倉庫每周四統(tǒng)一拼箱發(fā)貨，極大節(jié)約了物流成本，公司在為客戶提供良好服務(wù)的同時，也取得了飛速的發(fā)展。主要分類按用途壓力敏和力敏傳感器、位置傳感器、液位傳感器、能耗傳感器、速度傳感器、加速度傳感器、射線輻射傳感器、熱敏傳感器。按原理振動傳感器、濕敏傳感器、磁敏傳感器、氣敏傳感器、真空度傳感器、生物傳感器等。按輸出信號模擬傳感器：將被測量的非電學(xué)量轉(zhuǎn)換成模擬電信號。數(shù)字傳感器：將被測量的非電學(xué)量轉(zhuǎn)換成數(shù)字輸出信號（包括直接和間接轉(zhuǎn)換）。膺數(shù)字傳感器：將被測量的信號量轉(zhuǎn)換成頻率信號或短周期信號的輸出（包括直接或間接轉(zhuǎn)換）。開關(guān)傳感器：當(dāng)一個被測量的信號達(dá)到某個特定的閾值時，傳感器相應(yīng)地輸出一個設(shè)定的低電平或高電平信號。按其制造工藝集成傳感器是用標(biāo)準(zhǔn)的生產(chǎn)硅基半導(dǎo)體集成電路的工藝技術(shù)制造的。通常還將用于初步處理被測信號的部分電路也集成在同一芯片上。薄膜傳感器則是通過沉積在介質(zhì)襯底（基板）上的，相應(yīng)敏感材料的薄膜形成的。使用混合工藝時，同樣可將部分電路制造在此基板上。厚膜傳感器是利用相應(yīng)材料的漿料，涂覆在陶瓷基片上制成的，基片通常是Al2O3制成的，然后進(jìn)行熱處理，使厚膜成形。陶瓷傳感器采用標(biāo)準(zhǔn)的陶瓷工藝或其某種變種工藝（溶膠、凝膠等）生產(chǎn)。完成適當(dāng)?shù)念A(yù)備性操作之后，已成形的元件在高溫中進(jìn)行燒結(jié)。厚膜和陶瓷傳感器這二種工藝之間有許多共同特性，在某些方面，可以認(rèn)為厚膜工藝是陶瓷工藝的一種變型。每種工藝技術(shù)都有自己的優(yōu)點和不足。由于研究、開發(fā)和生產(chǎn)所需的資本投入較低，以及傳感器參數(shù)的高穩(wěn)定性等原因，采用陶瓷和厚膜傳感器比較合理。產(chǎn)品范圍：美國馬麥克MAMACSystems溫度傳感器TE-701美國馬麥克MAMACSystems傳感器、馬麥克MAMACSystems溫度傳感器、馬麥克MAMACSystems壓力傳感器、馬麥克MAMACSystems、馬麥克MAMACSystems濕度傳感器、馬麥克MAMACSystems熱電偶套管、馬麥克MAMACSystems恒溫器、馬麥克MAMACSystems電流開關(guān)主要型號：TE-701/702、TE-701BX、TE-705、TE-703/704、TE-211Z-213、TE-707、TE-205-F、TE-706-B、TE-205-EX、TE-205-EU、TE-205、A-505、A-500、PR-243、PR-274/275、PR-264、PR-276、PR-265、PR-282、A-520、A-523、HU-224/225、HU-226、HU-227、HU-921、HU-225、HU-225-EU、IP-PC-101-44-VDC、IP-PC-101-80-VDC、IP-MT-101、IP-MT-201、IP-MT-150、IP-SM-101、IP-PLE-85MAMACSystems成立于1981年，是傳感器，控制外設(shè)和網(wǎng)絡(luò)瀏覽器基于IP設(shè)備的領(lǐng)xian制造商。MAMAC產(chǎn)品可用于HVAC和環(huán)境控制，遠(yuǎn)程監(jiān)控報**，電能計量和工業(yè)自動化。MAMACSystems率先推出了HVAC環(huán)境控制、能源管理和輕型工業(yè)自動化市場。其率先推出低成本，溫度，濕度，壓力，流量傳感器和電流傳感器固態(tài)精密線。東莞市艾科有限公司供應(yīng)MAMACSYSTEMS/馬麥克；美國MAMACSYSTEMS；MAMACSYSTEMS溫度傳感器；MAMACSYSTEMS壓力傳感器；MAMACSYSTEMS濕度傳感器。還銷售以下產(chǎn)品：DOLANJENNER,SS53011006,SS748英寸升級含。SSEZ系統(tǒng)，120CM（48英寸標(biāo)稱值）和電源/繼電器組件（微）DOLANJENNER,SS20001066,SS9鏡，前表面窄66“DOLANJENNER,66001103005,SS7B系統(tǒng)24VDC48“布拉德斯和焊接SHIELDSDOLANJENNER,66000103201,SS7BSTD僅接收，24VDC15英寸DOLANJENNER,10061120000,SS-MTSSAFESCAN單個安裝組件（2-1/4IN隔離器及緊固件）DOLANJENNER,BXY6372,（部件編號：002114323072）單個柔性光纖（W/HYPO油管），長度=72。有源光纖直徑.063IN。不銹鋼護(hù)套170，1DOLANJENNER,BMY2772,單線圈DOLANJENNER,QVMABL72,（部件編號SK83560072）MINI面積BACKLIGHT，1.63“×1.86”活動區(qū)，72“電纜長度，PVC單線圈護(hù)套DOLANJENNER,MA2572,MINI環(huán)形燈DOLANJENNER,EL536T,單線圈DOLANJENNER,BUV436,（部件編號：002012014036）單光品位的石英光纖，長度=36。有源光纖直徑0.250。PVC單線圈護(hù)套170，180和3100DOLANJENNER,66000100204,SS7BSTD僅接收，115VAC35INDOLANJENNER,172F2,系統(tǒng)DUROUNITED,25-420-FG02L-0/100,表-范圍0-100PSI/0-7BAR100×KPADURO制造,MP-4,安裝板，四REEL（1400，1600，1800常規(guī)系列）DURO制造,3102,3100系列雙軟管敞開式（LESS軟管），1/4“或3/8”×75英尺到100英尺。DURO制造,2602,2500系列電線REELS30安培，REEL50FT。線的12/320安培DURO制造,1907年,1900系列雙支持基礎(chǔ)-單軟管卷盤（完整的軟管），1/4“X50英尺5000PSIWPDURO制造,1524,1500系列雙開喉轆（完整的軟管），1/2“X25”O(jiān)IL＆1/2“X25'油[詳細(xì)]
2018-10-06 10:02
產(chǎn)品樣冊
Managed systems dont lose their cool Managed systems dont lose their cool[詳細(xì)]
2024-09-18 18:06
專利
大鼠（Rat）IV型膠原（Co IV）ELISA試劑盒英文說明書 Normal07.8磅02falsefalsefalseMicrosoftInternetExplorer4Goatanti-RatCollagenTypeIVCollectSampleserumorbloodplasmaStorage:2-8°CPackagesize:96determinationsPRINCIPLEOFTHEMETHODTheCoIVkitisasolidphasephasesandwichenzymelinkedimmunosorbentassay（ELISA）.Samples,includingstandardsofknownCoIVconcentrationsandunknownsarepipettedintothesewells.Duringthefirstincubation,theCoIVantigenandabiotinylatedmonoclonalantibodyspecificforCoIVaresimultaneouslyincubated.Afterwashing,theenzyme（streptavidin-peroxydase）isadded.Afterincubationandwashingtoremovealltheunboundenzyme,asubstratesolutionwhichisactingontheboundenzymeisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofCoIVpresentinthesamples.REAGENTSPROVIDEDANDRECONSTTTUTIONREAGENTS（Storeat2-8℃）1×96WELLS0.5×96WELLSRECONSTTTUTION96/48-wellsmicrotiterplates10.5Ready-to-usePlastivcover21Ready-to-useStandard:800ng/ml1Vials（0.6ml）0.5Vials（0.3ml）Seereagentspreparationonpage3Blankcontrol1Vials（1.0ml）1Vials（0.5ml）Ready-to-useStandardDiluent1Vials（5ml）1Vials（2.5ml）Ready-to-useBiotinylatedanti-CoIV1Vials（6ml）1Vials（3.0ml）Ready-to-useStreptavidin-HRP1Vials（10ml）1Vials（5.0ml）Ready-to-useWashingBuffer1Vials（20ml）1Vials（10ml）50×concentrateSubstrateA1Vials（6.0ml）1Vials（3.0ml）Ready-to-useSubstrateB1Vials（6.0ml）1Vials（3.0ml）Ready-to-useStoppingSolution1Vials（6.0ml）1Vials（3.0ml）Ready-to-useSampleDiluent1Vials（12ml）1Vials（6.0ml）Ready-to-useMATERIALREQUIREDBUTNOTPROVIDEDlDistilledwaterlPipettes:10ul、50ul、100ul、200ul、1000ul。lVortexmixerandmagneticstirrer.SAFETYlForresearchuseonlylAvoidanyskincontactwithH2SO4andTMB.Incaseofcontact,washthoroughlywater.lDonoteat,drink,smokeorapplycosmeticswherekitreagentsareused.lDonotpipettebymouth.PROCEDURALNOTES/LAB.QUALITYCONTROLlWhennotinuse,kitcomponents首ldbestoredrefrigeratedorfrozenasindicatedonvialsorbottles.Allreagents首ldbewarmedtoroomtemperaturebeforeuse.Lyophilizedstandards首ldbediscardedafteruse.lOncethedesirednumberofstripshasbeenremoved,immediatelyresealthebagtoprotecttheremainingstripsfromedterioration.lCoverorcapallreagentswhennotinuse.lDonotmisorinterchangereagentsbetweendifferentlots.lDonotusereagentsbeyondtheexpirationdateofthekit.lUseacleandisposableplasticpipettetipforeachreagent,standard,orspecimenadditioninordertoavoidcross-contamination,forthedispensingofH2SO4andsubstratesolution,avoidpipetteswithmetalparts.lUseacleanplasticcontainertopreparethewashingsolution.lThoroughlymixthereagentsandsamplesbeforeusebyagitationorswir領(lǐng).lAllresidualwashingliquidmustbedrainedfromthewellsbyefficientaspirationorbydecantationfollowedbytappingtheplateforcefullyonabsorbentpaper.Neverinsertabsorbentpaperdirectlyintothewells.lTheTMBsolutionislightsensitive.Avoidprolongedexposuretolight,also,avoidcontactoftheTMBsolutionwithmetaltopreventcolourdevelopment.WarningTMBistoxicavoiddirectcontactwithhands.Disposeoffproperly.Ifadarkbluecolourdevelopswithinafewminutesafterpreparation,thisindicatesthattheTMBsolutionhasbeencontaminatedandmustbediscarede.Readabsorbanceswithin1houraftercompletionoftheassay.lWhenpipettingreagents,maintainaconsistentorderofadditionfromwell-to-well.Thiswillensureequalincubationtimesforallwells.lRespectincubationtimesdescribedintheassayprocedure.SPECIMENCOLLECTION\PROCESSINGANDSTORAGElSerum---Avoidanyinintentionalstimulationofthecellsbytheprocedure.Usepyrogen\endotoxinfreecollectingtubes.Serum首ldberemovedrapidlyandcarefullyfromtheredcellsafterclothing.Forthat,afterclothing,centrifugeatapproximately1000×gfor10minandremoveserum.lPlasma---EDTA\citrateandheparinplasmacanbeassayed.Spinsamplesat1000×gfor30minremoveparticulates.Harvestplasma.lCellculturesupernatants---Removeparticulatesandaggregatesbyspinningatapproximately1000×gfor10min.lStorage---Ifnotanalyzedshortlyaftercollection,samples首ldbealiquoted（250-500ul）toavoidfreeze-thawcyclesandstoredfrozenat-70℃.Avoidmultiplefreeze-thawcyclesoffrozenspecimens.Whenpossible,avoiduseofbadlyhemolyzedorlipemicsera.Iflargeamountsofparticlesarepresent,this首ldberemovedpriortoassaybycentrifugationorfiltration.lRecommendation---Donotthawbyheatingat37℃or56℃.Thawatroomtemperatureandmakesurethatsampleiscompletelythawedandhomogenousbeforeassaying.PREPARATIONOFREAGENTSlStandards:Standardhavetobereconstituledwiththevolumeofstandardbufferdiluentindicatedonthevial.Thisreconstitutionproducesastocksolutionof800ng/mlCoIV.Allowstandardtostandfor5lminuteswithgentleswir領(lǐng)priortomakingdilutions.Serialdilutionsofstandardmustbemadebeforeeachassysandcannotbestored.800ng/ml（6Standard）Originaldensity50ul。400ng/ml（5Standard）100ul6Standard+100uldiludent200ng/ml（4Standard）100ul5Standard+100uldiludent100ng/ml（3Standard）100ul4Standard+100uldiludent50ng/ml（2Standard）100ul3Standard+100uldiludent25ng/ml（1Standard）100ul2Standard+100uldiludent0ng/mlBlankControl50ul。lWashingbuffer50×concentrate:Dilute50timesindistilledwater.ASSAYMETHODlBeforeuse,mixallreagentsthoroughlywithoutmakingfoam.lDeterminethenumberofmicrowellstripsrequiredtotestthedesirednumberofsamples,plusappropriatenumberofwellsneededforrunningblanksstandards.Eachsample,standardandblank首ldbeassayedinduplicate.Removesufficientmicrowellstripsfromthepouch.lAdd50ulofstandarddiluenttostandardwellsB1,B2,C1,C2,D1,D2,E1,E2,F1,F2.Reconstitutestandardvialwiththeappropriatevolumeasdescribedinthechapterreagentspreparation.Preparation.Pipet100ulofstandardintowellsA1andA2（seeplateschemebelow）.Transfer50ulfromA1andA2toB1andB2wells.Mixthecontentsbyrepeatedaspirationsandejections.Takecarenottoscratchtheinnersurfaceofmicrowells.RepatthisprocedurefromthewellsB1,B2towellsC1,C2andfromwellsC1,C2toD1,D2andsooncreatingtwoparallelrowsofCoIVstandarddilutionsranging，Add50ulofstandarddiluenttotheblandwells.lDilutesamples1:1distribing50ulofsampleinto50ulofdilluent,Add50ulofdilutedsampletowells..lAdd50ulofdilutedbiotinylatedanti-CoIVtoallwells.lCoverwithaplatevoverandincubatefor1hourat37℃.lRemovethecoverandwashtheplateasfollows:⑴ aspiratetheliquidfromeachwell,⑵ dispensse0.3mlofwashingsolutionintoeachwell.⑶ Aspirateagainthecontetofeachwellafter0.5minute.⑷ Repeatsteps⑵and⑶threetimes.lDistribute80ulofstreptavidin-HRPsolutiontoallwells,includingblankwells.lCoverandincubate30minat37℃.lRemovethecoverandemptywells,Washmicrowellstripsaccordingtostep,Proceedimmediatelytothenextstep.lAdd50ulSubstrateAandSubstrateBtoeachwell。Incubatefor10minat37℃。lTheenzyme-substratereactionisstoppedbyquicklypipetting50ulofH2SO4.stopreagentintoeachwell,includingtheblankwells,tocompletelyanduniformlyinactivatetheenzyme.ResultsmustberedimmediatelyaftertheadditionofH2SO4.lReadabsorbanceofeachwellonaspectrophotometerusing450nmastheprimarywavelengthandoptionally620nm（610nmto650nmisacceptable）asthereferencewavelength.SUGGESTEDPLATESCHEMEStandardconcentrations（ng/ml）A800800samplesamplesamplesamplesamplesamplesamplesamplesamplesampleB400400samplesamplesamplesamplesamplesamplesamplesamplesamplesampleC200200samplesamplesamplesamplesamplesamplesamplesamplesamplesampleD100100samplesamplesamplesamplesamplesamplesamplesamplesamplesampleE5050samplesamplesamplesamplesamplesamplesamplesamplesamplesampleF2525samplesamplesamplesamplesamplesamplesamplesamplesamplesampleG00samplesamplesamplesamplesamplesamplesamplesamplesamplesampleHsamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesamplesampleLIMITATIONSOFTHEPROCEDUREDonotextrapolatethestandardcurvebeyondthemaxstandardcurvepoint.Thedose-responseisnon-linearinthisregionandgoodaccruacyisdifficulttoobtain.CALCULATIONOFRESULTSTheminimumdetectableconcentrationinthisassayisestimatedtobe1.0ng/ml[詳細(xì)]
2018-11-16 10:02
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大鼠IV型膠原（Collagen IV）ELISA試劑盒說明書
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