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AutoMet? Grinder-Polisher System
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The AutoMet grinder polishers are high performance machines designed for reliability, flexibility, and ease of use.
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AutoMet? Grinder-Polisher System
The AutoMet grinder polishers are high performance machines designed for reliability, flexibility, and ease of use.[詳細(xì)]
2021-02-23 10:56
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EcoMet. 30 Grinder-Polisher Systems
The EcoMet 30 family provides simple operation for routine grinding and polishing.[詳細(xì)]
2021-02-23 10:44
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MiniMet? 1000 Semi-Automatic Grinder-Polisher
The MiniMet 1000 Grinder/Polisher is designed for low volume laboratories preparing single specimens.[詳細(xì)]
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Burst Dispensing System
With an already flourishing grinder-polisher family, Buehler has expanded and simplified capabilities by introducing the BurstDispensing System.[詳細(xì)]
2021-02-22 21:34
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Apex. S System
The Apex S Quick Change System improves productivity and usability of Grinding-Polishing machines through simple application and effortless removal of grinding surfaces to the working platen.[詳細(xì)]
2021-02-23 10:51
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The Solartron CellTest? System
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BDRevTetSystem使用說(shuō)明pRevTet-On,pRevTet-Off,pRevTet-Off-IN,pRevTREIntroductionTheBDRevTet-OnTM&BDRevTet-OffTMSystemscombinetheadvantagesofretrovirus-mediatedgenetransferwiththeproventetracycline-regulatedcontrolofBDTet-OnTMandBDTet-OffTMGeneExpressionSystems.BDRevTetTMallowsthefastandefficientestablishmentofregulatedgeneexpressionsystemsinawidevarietyofcelltypes.EachRevTetSystemisacompleteretroviralgeneexpressionsystemcontainingaretroviralregulator(r)tTAvector,aretroviralresponsevector,acontrolvector,andapackagingcellline.LikeourBDRetro-XTMSystem(#631508),theRevTetSystemscontaintheBDRetroPackTMPT67PackagingCellLine(#631510)forthesafe,efficientproductionofinfectious,replication-incompetentretrovirus.TheRevTetSystemsprovideapowerfulandconvenientapproachtosettingupthetetracycline(Tc)-inducible,high-levelgeneexpressionsystemsfirstdescribedbyGossenandBujard(1992).Retroviral-mediatedtet-regulationTheTetSystemsarebasedontwoelementsfromthetetoperonoftheE.coliTn10transposontheTetrepressorprotein(TetR)andthetetoperatorDNAsequence(tetO).ThegenetobeexpressedisclonedintothepRevTREresponsevectordownstreamofthetetracycline-responsiveelement(TRE),whichcontainssevendirectrepeatsofthe42-bptetOsequenceandtheminimalimmediateearlypromoterofcytomegalovirus(PminCMV).Thetwosystemsdifferintheirregulatoryvector(Figure1).TheRevTet-OffSystemusesthepRevTet-OffVector,whichcontainsthetTAregulatoryelement,afusionofTetRandtheVP16activationdomain.TheBDRevTet-OnTMSystemusespRevTet-On,basedonthereversetTA(rtTA).BothsystemsusethepRevTREresponsevector.AlltheRevTetVectorsarederivedfrompLNCX,aBDRetro-XTMvectorcapableofproducinghigh-titervirusintheappropriatepackagingcelllines.IntheRevTet-OffSystem,tTAbindstheTREandactivatestranscriptionintheabsenceofTcortheTcderivativedoxycycline(Dox).Thus,asTcorDoxisaddedtotheculturemedium,transcriptionisturnedoffinadose-dependentmanner(Figure1).Conversely,theRevTet-OnSystemallowsgeneexpressiontobeactivatedbytheadditionofDox.Thesetwocomplementarysystemsallowyoutochoosetheonethatbestsuitsyourneeds.BenefitsandapplicationsofTc-mediatedinductionInmostinduciblemammaliangeneexpressionsystems(e.g.,inductionbyheavymetals,steroidhormones,orheatshock),inductionisnonspecificandexpressionlevelscannotbepreciselyregulated.Inaddition,thesesystemsaregenerallyleakyinthe“off”state,andtheinducingagentitselfmaybecytotoxicorhavepleiotropiceffectsonresults.Incontrast,regulationofgeneexpressionbytheheterologousbacterialcontrolelementsintheRevTetSystemsisveryspecific.Furthermore,thelevelsofTcorDoxrequiredforthefullrangeofgeneexpressionarenotcytotoxicandhavenosignificanteffectoncellproliferationoranimalgrowth,evenwithcontinuoustreatment(Bohletal.,1997;Mayfordetal.,1996).TheuseofseparateregulatoryandresponsevectorsintheRevTetSystems質(zhì)粒圖譜:[詳細(xì)]
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