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Invitrogen GeneSwith 哺乳動物誘導(dǎo)系統(tǒng)pSwitch 說明書
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InvitrogenGeneSwith哺乳動物誘導(dǎo)系統(tǒng)InvitrogenVectors:pSwitch產(chǎn)品參數(shù):MammalianSelection:Hygromycin載體抗性:氨芐青霉素(Ampicillin)載體描述:Switch-OnExpressionfromtheLowestBasalLevelsTheGeneSwitch?Systemforinduciblemammalianexpressionisidealforexperimentsthatrequiretheabsolutelowestuninducedexpressionlevels.TheexpressionvectorpGene/V5-Hisprovidesaminimalpromoter,GAL4-E1b,consistingofthebindingsitesfortheyeastGal4DNAbindingproteinfollowedbytheTATAsequencefromtheAdenovirusE1bpromoter.Withoutadditionalfactors,theGAL4-E1bpromoteristranscriptionallysilent.ToactivatetranscriptionfromtheGAL4-E1bpromoter,theGeneSwitch?regulatoryproteinisexpressedfromaminimalTKpromoteronthepSwitchvector.TheGeneSwitch?proteinhasthreefunctionaldomains:Gal4DNABindingDomain(Gal4-DBD)tobindtheregulatoryproteintotheGAL4-E1bpromoterTruncatedhumanProgesteroneReceptorLigandBindingDomain(hPR-LBD)thatundergoesaconformationalchangewhenitbindstheprogesteroneantagonist,mifepristoneTranscriptionactivationdomainfromtheNF?Btranscriptionfactorp65(p6D)toactivatetranscriptionfromthesilentGAL4-E1bpromoterIntheabsenceofmifepristone,theconformationofthehPR-LBDregionpreventstheGeneSwitch?regulatoryproteinfromactivatingtranscriptionfromtheGAL4-E1bpromoter.WhenmifepristoneisaddedandbindsthehPR-LBDregion,theGeneSwitch?regulatoryproteinassumesaconformationthatpermitsittostimulatetranscriptionfromtheGAL4-E1bpromoter.InductionoftheGeneSwitch?SystemleadstoactivationofthegeneofinterestonthevectorpGene/V5-His.Inaddition,fourGal4bindingsitesupstreamoftheminimalHSVTKpromoteronpSwitchcanbindthepSwitchregulatoryprotein.Therefore,addingmifepristoneup-regulatesproductionoftheregulatoryprotein.TheincreasedlevelsoftheGeneSwitch?regulatoryproteinresultininductionofthegeneofinterestfrompGene/V5-Histolevelsthatcanapproachthoseofviralpromoters.TheGeneSwitch?Kitoffersexceptionallylowuninducedandhighinducedexpressionlevelsinmammaliancells.ThesystemprovidestheexpressionvectorpGene/V5-Hiswiththeminimal,syntheticGAL4-E1bpromoterthatistranscriptionallysilentuntilactivated.TheGeneSwitch?regulatoryproteinbindstheGAL4-E1bpromotertoactivatetranscriptionupontheadditionofmifepristone.Inaddition,theGeneSwitch?proteinupregulatesitsownexpression,leadingtoamplifiedexpressionofthegeneofinterestfrompGene/V5-His.pGene/V5-Hisoffersseveralfeaturesthatfacilitateexpressionanalysisandpurificationofrecombinantproteinsinmammaliancells:C-terminalV5epitopetagfordetectionwithAnti-VntibodiesC-terminalpolyhistidine(6xHis)tagforrapidpurificationwithnickel-chelatingresinanddetectionwithAnti-His(C-term)AntibodiesTheZeocin?resistancegeneforeffectiveselectioninbothmammaliancellsandE.coli
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- Invitrogen博萊霉素促銷產(chǎn)品簡介Zeocin博萊霉素產(chǎn)品編號規(guī)格價格Z8020125mg/1.25ml600.00說明:Zeocin是一種屬于爭光霉素家族的糖蛋白抗生素,在體內(nèi)能作用于大多數(shù)細菌(包括E.coli)、真菌(如:酵母菌)、植物細、動物細胞。分子式:C55H83N19O21S2Cu分子量:1137.41外觀:淡-綠色液體儲存條件:-20℃避光FromInvitrogenR250-01Invitrogen博萊霉素促銷特價促銷125mg/1.25ml600元熱線電話【021-54100800】其他產(chǎn)品附錄:CAS號貨號solarbio品名產(chǎn)地規(guī)格S0083槲皮素標準品solarbio20mg146-68-9I8150碘硝基氯化四氮唑藍(INT)進分250mg79-14-1G8840羥基乙酸glycolicacid進分100g134-03-2S9440Sodiumascorbate抗壞血酸鈉solarbio25g13573-16-5R8160Reineckesalt雷氏鹽(利英納克鹽)solarbio25g120-72-9I8190Indole吲哚solarbio10G1465-25-4N8320N-1-萘基乙二胺鹽酸鹽solarbio5G6409-77-4C8850Nuclearfastred核固紅進分5G60-92-4C8860cAMP環(huán)磷酸腺苷solarbio1G259962L8100水解乳蛋白BactoTMTCLactalbuminHydrolysateBD500g259962L8100水解乳蛋白BactoTMTCLactalbuminHydrolysateBD100G620-45-1D87102.6-Dichorophenoindophenol,Na2.6二氯酚靛酚鈉alfa1G148-24-3H82608-Hydroxyquinoline8-羥基喹啉Solarbio25g68157-60-8F8080-1Forchiorfennron氯吡苯脲(KT-30cppu)國產(chǎn)1G51-21-80597F83005-Fluoro-2'-Deoxyuridine5-氟尿嘧啶amresco1G12771-68-9431Ancymidol環(huán)丙嘧啶醇Sigma原裝25MG153-18-4R8170Rutinhydrate蕓香葉苷(蘆?。﹪a(chǎn)25G6151-25-3Q8010Meletin或Sophretin.槲皮素國產(chǎn)10G7761-88-8S8480Silvernitrate硝酸銀國產(chǎn)10G102129-65-7A8760ADPG腺苷二磷酸葡萄糖sigma10MG21931-53-3U81005-UDP(尿苷-5-二磷酸)solarbio100MG28053-08-9U4625U8080UDPG尿苷5-二磷酸葡萄糖二鈉sigma100MG100684-32-0M0630M8090Myoglobin肌球蛋白(肌紅蛋白)sigma100mg9007-83-4G5009G8410V-Globulinsfrombovinebloodv-球蛋白sigma1g9010-66-6Z3625Z8010Zein玉米蛋白sigma25gXAD7Amberlite?XAD7HP吸附樹脂(20-60目)sigma100g優(yōu)質(zhì)試劑質(zhì)量放心價格Zdi為ZG生物產(chǎn)業(yè)做貢獻ShanghaisolarbioBioscience&TechnologyCo.,LTD[詳細]
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2018-09-02 10:00
產(chǎn)品樣冊
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TRIzol Reagent (invitrogen 貨號15596-026)說明書
- 詳細說明常用規(guī)格ShippingCondition:RoomTemperatureSampleType(General):ViralSamples,Tissue,Blood,Bacteria,Yeast,Cells,PlantSamplesFinalProduct:TotalRNA,microRNA,TranscriptomeRNADownstreamApplication:Real-TimePCR,cDNALibraryConstruction,NorthernBlotting,NucleaseProtectionAssays,RT-PCR(Endpoint),CloningHighThroughputCompatibility:ManualProtocols,NotHighThroughput-CompatibleIsolationTechnology:OrganicExtractionScale:ScaleableSystem:TRIzolQuantity:1ProductSize:100mlYield(upto):8mgPurificationTime:1hrNumberofReactions:100PrepsStartingMaterial(Amount):Upto1gtissue,Upto1x10^7cellsRegulatoryStatement:ForResearchUseOnly.Notforuseindiagnosticprocedures.[詳細]
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2018-10-02 10:03
產(chǎn)品樣冊
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invitrogen 2012價目表
- 上海北諾生物科技有限公司www.bnbiotech.com上海市徐匯區(qū)宜山路520號中華門大廈18樓D座電話:021-61496710傳真:021-57633753聯(lián)系人:周經(jīng)理,劉經(jīng)理上海北諾生物科技有限公司成立于2007年,是由留美生物醫(yī)學(xué)界專家與國內(nèi)生命科學(xué)界知名專家共同創(chuàng)辦,由中科院健康科學(xué)研究所、上海市免疫學(xué)研究所、上海市內(nèi)分泌研究所、上海市血液學(xué)研究所、上海交通大學(xué)、上海交通大學(xué)醫(yī)學(xué)院附屬瑞金醫(yī)院等生命科學(xué)研究專家提供技術(shù)支持和理論指導(dǎo)。北諾生物精選國際yi流品牌和國內(nèi)經(jīng)過認證的、質(zhì)量較為可靠、有質(zhì)量體系管理的產(chǎn)品供應(yīng)商的產(chǎn)品,能夠根據(jù)課題需要,提供基于免疫學(xué)、分子生物學(xué)和細胞生物學(xué),微生物學(xué)產(chǎn)品組合。通過進口產(chǎn)品和國內(nèi)精選、質(zhì)量可靠的產(chǎn)品組合,保證您的課題能順利、甚至高質(zhì)量完成。如果北諾生物沒有您需要采購的產(chǎn)品,我們也會向您推薦優(yōu)質(zhì)供應(yīng)商,讓您自行選擇和參考。北諾生物作為領(lǐng)xian的專業(yè)科研整體試劑服務(wù)提供商。匯聚了具有嚴格質(zhì)控體系的試劑供應(yīng)商及經(jīng)驗豐富的生物專業(yè)學(xué)術(shù)人員,致力于為ZG醫(yī)學(xué)與生物科研提供Z專業(yè)的生物試劑咨詢導(dǎo)購,節(jié)省您的實驗時間和經(jīng)費,為您的論文發(fā)表保駕護航!公司初期的目標是建立國內(nèi)生命科學(xué)研究提供快速供貨系統(tǒng),我們正在建設(shè)全備的生命科學(xué)研究試劑現(xiàn)貨供應(yīng)ZX,為生命科學(xué)研究的快速發(fā)展服務(wù)。公司的Z終目標是在生命科學(xué)和醫(yī)學(xué)科學(xué)研究領(lǐng)域創(chuàng)造具有國際競爭力的自主知識產(chǎn)權(quán)品牌產(chǎn)品,力爭打破歐美生物技術(shù)公司在生命科學(xué)領(lǐng)域一統(tǒng)天下的局面,[詳細]
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2018-10-14 10:00
產(chǎn)品樣冊
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LIBS 激光誘導(dǎo)解析光譜系統(tǒng)
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2024-09-28 02:52
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什么是激光誘導(dǎo)激光光譜系統(tǒng)?激光誘導(dǎo)擊穿光譜技術(shù)特點
- 激光誘導(dǎo)擊穿光譜(LIBS)是一種原子發(fā)射光譜儀。可以對固相、液相和氣相基體中幾乎所有元素進行定性和定量的分析。不同于傳統(tǒng)的檢測方法如ICP-OES或者XRF,LIBS在檢測過程中無需進行復(fù)雜的樣品制備。為了達到這個目的,LIBS采用高能量聚焦脈沖激光光束將樣品激發(fā)至等離子態(tài),對產(chǎn)生的對應(yīng)元素發(fā)射譜進行分析。元素發(fā)射譜的波長與元素的種類直接相關(guān),而元素譜線的強度則和元素的含量相關(guān)。[詳細]
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2024-09-30 23:10
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哺乳動物或攜帶32萬種未知病毒
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2024-09-23 20:44
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LIBS 激光誘導(dǎo)擊穿光譜系統(tǒng)應(yīng)用案例
- 土壤&農(nóng)作物污染檢測;
古玩鑒定;
煤炭&金屬測量;
等離子體發(fā)光測量[詳細]
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2024-09-10 22:57
應(yīng)用文章
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原裝invitrogen進口Zeocin 博來霉素|價格
- 現(xiàn)貨供應(yīng)Zeocin博來霉素|原裝進口|價格產(chǎn)品簡介:Zeocin可選擇Shble基因表達的細胞。與現(xiàn)在使用的其他動物細胞標記物無交叉抗性。因此這種抗生素可用來分離對其他篩選劑(如:慶大霉素,潮霉素)有抗性的克隆。Zeocin是一種屬于爭光霉素家族的糖蛋白抗生素,在體內(nèi)能作用于大多數(shù)細菌(包括E。coli)、真菌(如:酵母菌)、植物細胞、動物細胞、細胞培養(yǎng)級?,F(xiàn)貨供應(yīng)Zeocin博來霉素3015元特價【021-54100800】Zeocin博來霉素上海索寶特價【021-54100800】產(chǎn)品明細:Zeocin試劑是博來霉素抗生素家族的一個成員。其抗性由編碼一種13,665道爾頓蛋白質(zhì)的Shble基因賦予。在表達這種蛋白質(zhì)的細胞中,Zeocin無法結(jié)合和切割細胞DNA。Zeocin對大多數(shù)好氧細胞有效,可用于選擇哺乳動物和昆蟲細胞系、酵母以及細菌。選擇細胞時,Zeocin的用量為50-2000ug/ml(通常300ug/ml),具體取決于細胞類型?;瘜W(xué)式:C55H83N19O21S2Cu形式:液體代理:zeocin產(chǎn)品規(guī)格:8×1.25毫升試劑類型:抗生素(選擇性)濃度:100毫克/毫升驗證應(yīng)用程序:的真核生物選擇/穩(wěn)定細胞系生成,細菌的選擇監(jiān)管聲明:僅用于科研。不為任何動物或人類ZL或診斷用?,F(xiàn)貨供應(yīng)Zeocin博來霉素上海索寶特價【021-54100800】Zeocin博來霉素上海索寶特價【021-54100800】ShanghaisolarbioBioscience&TechnologyCo.,LTD上海索萊寶生物科技有限公司是一家集銷售生化試劑、實驗耗材、自產(chǎn)分子生物學(xué)產(chǎn)品為一體,并能提供技術(shù)支持的專業(yè)性生物科技公司。公司秉承“以客戶為ZX,以產(chǎn)品為保障、以誠信為基礎(chǔ)、以創(chuàng)新為宗旨”的發(fā)展理念,在依靠客戶的大力支持和員工的不懈努力下獲得了快速的成長。公司組織結(jié)構(gòu)清晰,內(nèi)部管理科學(xué),擁有一支既分工細致又高度合作的年輕化隊伍,保持了各個部門之間的GX合作運轉(zhuǎn),充分保障了公司在充滿競爭的市場中處于領(lǐng)xian地位。公司注重與業(yè)界精英合作,目前公司已經(jīng)和Amersham、Amresco、Axygen、BD、Corning、Dragon、Eppendorf、等企業(yè)結(jié)成緊密的合作伙伴關(guān)系,代理其領(lǐng)xian世界的產(chǎn)品,并在全國各地設(shè)有分銷機構(gòu)。我們將以高度的責任感和出色的產(chǎn)品質(zhì)量為客戶提供高品質(zhì)的產(chǎn)品和實驗技術(shù)解決方案。豐富的經(jīng)驗、優(yōu)良的品質(zhì)、充足的庫存、準確的交貨時間、極具競爭力的價格,所有這些都保證了我們向您提供的是Z專業(yè)Zyou質(zhì)的服務(wù)!立足北京,上海,輻射全國,隨著產(chǎn)品營銷戰(zhàn)略的不斷延伸,我們將逐步發(fā)展成為yi流的專業(yè)性生物科技公司。我們希望能夠和尊敬的客戶一起,利用各自的資源優(yōu)勢和勇于進取的敬業(yè)精神,為ZG生命科學(xué)研究的發(fā)展作出更多的貢獻。為了更好、更全面的發(fā)展,現(xiàn)公司正在誠招各地dai理商和招聘若干銷售、研發(fā)人員,歡迎垂詢并期待您的加入!現(xiàn)貨供應(yīng)Zeocin博來霉素上海索寶特價【021-54100800】聯(lián)系方式上海索萊寶生物科技有限公司電話:021-6485566518018609966傳真:021-54261506郵編:200233地址:上海市欽江路15號14層郵箱:solarbio@126.com網(wǎng)址:www.shsolarbio.com優(yōu)質(zhì)試劑質(zhì)量放心價格Zdi為ZG生物產(chǎn)業(yè)做貢獻現(xiàn)貨供應(yīng)Zeocin博來霉素上海索寶特價【021-54100800】手機18018609966[詳細]
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2018-09-02 10:00
產(chǎn)品樣冊
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invitrogen原裝產(chǎn)品 促銷目錄|價格
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2018-09-02 10:00
產(chǎn)品樣冊
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