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孔雀石綠試劑盒(水樣)
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孔雀石綠試劑盒(水樣)
- 孔雀石綠試劑盒(水樣)[詳細(xì)]
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2024-10-01 12:16
報價單
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孔雀石綠檢測卡(水樣)
- 孔雀石綠檢測卡(水樣)[詳細(xì)]
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2012-08-24 00:00
課件
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LCMS分析孔雀石綠隱性孔雀石綠
- LCMS分析孔雀石綠隱性孔雀石綠[詳細(xì)]
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2010-06-13 00:00
產(chǎn)品樣冊
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HPLC 法分析孔雀石綠和無色孔雀石綠
- HPLC 法分析孔雀石綠和無色孔雀石綠[詳細(xì)]
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2013-05-13 00:00
安裝說明
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水產(chǎn)品中孔雀石綠和無色孔雀石綠的測定
- 水產(chǎn)品中孔雀石綠和無色孔雀石綠的測定[詳細(xì)]
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2011-07-06 00:00
實驗操作
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孔雀石綠檢測卡(組織)
- 孔雀石綠檢測卡(組織)[詳細(xì)]
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2012-08-24 00:00
實驗操作
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孔雀石綠英文說明書.pdf
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malachitegreen(MG)ELISA1UsepurposeThiskitforFeed,aquaticsamples,watersamplesintheMGremainingquantitativedetection.2ExperimentalprincipleThiskitadoptsmethodsincompetitionELISAInmicroplatescoatedwithantigenconjugatedMG,addMGstandardorsamples,FreeMGandpre-coatedonstripsofMGconjugatedantigencompeteagainstMGantibodyconjugates,WithTMBchromogenicsubstrate,thecolorischangedfrombluetoyellowafteraddingstopsolution,enzymestandardinstrument450nmwavelengthsintesting,absorblightvalueandinthesamplewasMGcontentisinverselyproportionaltothestandardcurve,throughcalculationsampleswasMGconcentrations.3Materialsprovidedwiththekit3.1Microelisastripplate:1block(12well×8strips).3.2MGstandard:sixvialsof(1ml/vial),contentisrespectively:0PPB,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB.3.3Anti-MGantibodyconjugate:1vial(6ml).3.4ChromogenSolutionA:1vial(6ml).3.5ChromogenSolutionB:1vial(6ml).3.6StopSolution:1vial(6ml),2Msulphuricacid.3.7sampledilution:1vial(10x,6ml),usedforsampledilutedwith.3.8washsolution:1vial(20x,20ml),usedforwashingboard.3.9Instruction.4Neednotprovidematerials4.1equipment4.4.1wavelength450nmmicroplate.4.1.2shredder.4.1.3LiangTong.4.1.4oscillators.4.1.5funnel.4.1.6WhatmanNo1orequivalentfilterpaper.4.1.7traceremoveliquiddevice.4.2reagent4.2.1thedeionizedwaterordistilledwater.4.2.2methanol.5Storage5.1kitstoredin2~8℃,Don’tfrozen5.2Don’tuseuptheMicroelisastripplate首ldbesealeddryingpreserve6.Precautions6.1pleasereadtheinstructionscarefully,beforeuseingthekit.6.2don'tuseexpiredkit.6.3beforeusingthekit,pleaseletthereagentrecovertoroomtemperature(25+2℃),theproposalforatleast2hourstotemperature.6.4thestandardcontainMG,payspecialattentionto,theoperation,we首ldbringgloves.6.5stopsolutioniscontainingsulfate,whenusing,preventburnsskinandcorrosionclothing.6.6differentstandardandsamplesuctionheadusedcannotbemixeduse,otherwise,itwillaffecttestresults.6.7differentbatchesofreagentkitnotmix,Differentstandardandsamplesuctionheadshallnotbeusedincombination,otherwise,itwillaffecttheexperimentalresult.6.8dilutedsamplemustusethiskitofsamplediluent,otherwise,itwillaffecttheexperimentalresult6.9mixedreagents首ldavoidblistering.7Workingliquidpreparation7.1Carbendazimstandards:0ppb,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB7.2washsolution:1:20withdistilledwater(1+19)diluted.preparation7.3sampledilutions:1:10withdistilledwater(1+9)diluted.preparation7.3ChromogenSolutionreagent:alreadystandby,avoidlightstraightas7.4stopsolution:alreadyterminationaside8Sampleprocessingprogram(sampleinextractionprocess,muststrictlyaccordingtotheoperationoftheextractionprocess首ldbeaccuratedilution,canappearotherwiseresultsareinaccurate,samples首ldbekeptinacoolplacetoavoidlightandfrozenkeep)8.1Smashthesamplestaken10g,add20ml70%methanolsolution8.2powerfuloscillation3minutes8.3WhatmanNo1filterwith8.4Takethe25μltreatmentofthesamplebyadding25μlsampledilutioninthewells(sampledilutionfactorof2)9Enzyme2lindedanalysissteps9.1experimentalguidelines9.1.1experimentbeginspriortoallreagentinboxesoutsidetheroomtemperature(25fullyrecoveredto+2℃),timeabout2hours.Returntoroomtemperature(25+2℃)againafterremoveStrips,excessporebartosealimmediatelytothe2~8℃dryingpreserveNote:besuretotemperature,otherwisefullyguaranteetheaccuracyandprecisionoftheaffectingdetection.9.1.2afterusepleaseimmediatelyreagentputback2~8℃preservation9.1.3pleasedon'tchangeanalysisprogram9.1.4pleaseuseaccuratetraceremoveliquiddevice9.1.5operationoncestarted,pleasedonotinterruptanyprogram9.1.6ELISAresultsofrepeatabilityofseveredependsonoperatingprocedures,pleasestrictlyaccordingtorequirementsoperation9.1.7toavoidcross-contamination,eachstandardandsamples首ldusedifferentsuctionheadaddsamples9.1.8plussampledomakesuckaheadcontactmicroporousthesolutionorinsidesurface9.2analysissteps9.2.1beforehandnumbered,markB0,standardandthesamplepositionrecommendeddoubleorificedetection9.2.2taketherequiredamountoftheStrips(Stripsdetachable),willspareattribwattleandsealedimmediatelyputbackagain2~8℃preservation9.2.3sampledilutions(10),washsolutionx(20x)dilutionintoworkingliquid(distilledwaterordeionizedwaterdilute)In9.2.4B0welljoining50μl0.0ng/mlstandard9.2.5ineachstandardwelljoining50μlstandard9.2.6ineachsamplewelljoining50μlsamplesolution9.2.7Inallwelljoining50μlAnti-MGantibodyconjugate9.2.8gentlysloshingresponseboardforafewseconds.9.337℃warmbath30min(warmbathprocesssometimespatreactionplate,canreducedoubleorificeerror)9.3.1UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat..9.4reaction9.4.1washingprocedurecompleted,immediatelywithliquidapparatusineverytracemovemicroporousfirstjoin50μlChromogenSolutionA,add50μlChromogenSolutionB,Slightsloshingresponseboardmakethoroughlyincorporated379.4.2℃warmbath10min9.4.3eachwelljoining50μlStopSolution,blending9.4.4in450nmtestingabsorbency,resultin5mininsideread.10Theresultscalculated10.1quantitativeanalysis10.1.1obtainedbyeachconcentrationstandardsolutionandtheaveragevalueofasamplespectrophotometry(B)dividedbythefirststandard(0standardabsorbencyvalue(B0)multiplyingby1**%,namelypercentageabsorbencyvalues.B-standardsolutionorsamplesolutionofaverageabsorbanceofthevaluesB0-0μg/Lstandardsolutionofaverageabsorbanceofthevalues10.1.2withMGconcentrationsofvaluesfortheXaxis,100centabsorbanceofthevalueoftheYaxis,drawstandardcurve.Accordingtothesamplepercentageabsorbencyvalues,whichgetcorrespondingpointsfromcurve,namelytheabscissadenotesthemulti-goalMGconcentrationonthenumericalcurvature.theagainstseveralnamelytodetermineMGconcentrationC(ppb)10.1.3becausethesampleafterdilutedinadvance,soaccordingtothestandardcurvegainsfromdifferentconcentrationsamplesmustagainmultiplythedilutedtimes.10.2halfquantitatively10.1.1visualhalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesamplesandstandardsubstanceabsorbencyvaluethediscretionofthejudgeiscompared,samplechromavalueislessthanorgreaterthanstandardvalues.10.1.2instrumenthalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesampleandstandardcolordepthcomparison,judgesamplechromavalueislessthanorgreaterthanstandardvalues.11SpecificityPhysicalcrossreactionMG1**%CrystalViolet:95%Recessivemalachitegreen:0.1%HiddenCrystalViolet:<0.1%12KitparametersThiskitdetectionlimitis0.05PPBB0absorbanceoftheoptimalvalue首ldbegreaterthan1.0Kitabsorbencyboardinsideerrorislessthan8%,boardbetweenerrorislessthan15%.Withthismanualprovidedatissuesampleextractionmethodrecoveryisgreaterthan80%.13AnalysisrestrictionThiskittestingpositiveforsamples首lduseanothermethodsuchasHPLCorGC/MStobeverified.上海恒遠(yuǎn)生物科技有限公司,專業(yè)銷售“孔雀石綠試劑盒”品質(zhì)保證,值得信賴!如果你想了解該產(chǎn)品的詳細(xì)信息,歡迎來電來函![詳細(xì)]
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2018-11-15 10:03
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LCMS串聯(lián)四極質(zhì)譜檢測食品中的 孔雀石綠和隱性孔雀石綠
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2024-09-28 11:33
選購指南
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4551A型水樣自動進(jìn)樣器
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2008-09-29 00:00
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水樣a放射性濃度計算公式
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2018-10-07 10:02
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孔雀石綠和結(jié)晶紫標(biāo)準(zhǔn)品
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2010-12-08 00:00
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適用于高效液相色譜法測定水產(chǎn)品中孔雀石綠和隱色孔雀石綠殘的留量
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2020-04-26 17:54
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2018-09-24 10:00
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2024-09-30 09:25
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LC5500高效液相色譜儀測定水產(chǎn)品中孔雀石綠
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2006-01-26 00:00
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