本文由 北京華安麥科生物技術有限公司 整理匯編

2012-08-24 00:00 373閱讀次數(shù)

收藏 評價 舉報

• 分享

立即下載

參與評論

評論

登錄后參與評論

登錄或新用戶注冊

• 微信登錄
• 密碼登錄
• 短信登錄

請用手機微信掃描下方二維碼
快速登錄或注冊新賬號

微信掃碼，手機電腦聯(lián)動

注冊登錄即表示同意《儀器網服務條款》和《隱私協(xié)議》

賬  號：

密  碼：

圖片碼： 看不清？
換一張？

手機和郵箱號注冊新賬號>> 忘記密碼？

手機號：

圖片碼： 看不清？
換一張？

短信碼： 獲取短信碼

手機和郵箱號注冊新賬號>> 忘記密碼？

更多資料

• 孔雀石綠檢測卡（組織）

  孔雀石綠檢測卡（組織）[詳細]

  2012-08-24 00:00

  實驗操作

  |

  • 
  • 
  • 
  • 

• 孔雀石綠檢測卡（水樣）

  孔雀石綠檢測卡（水樣）[詳細]

  2012-08-24 00:00

  課件

  |

  • 
  • 
  • 
  • 

• LCMS分析孔雀石綠隱性孔雀石綠

  LCMS分析孔雀石綠隱性孔雀石綠[詳細]

  2010-06-13 00:00

  產品樣冊

  |

  • 
  • 
  • 
  • 

• HPLC 法分析孔雀石綠和無色孔雀石綠

  HPLC 法分析孔雀石綠和無色孔雀石綠[詳細]

  2013-05-13 00:00

  安裝說明

  |

  • 
  • 
  • 
  • 

• 快速檢測卡

  快速檢測卡[詳細]

  2012-08-23 00:00

  產品樣冊

  |

  • 
  • 
  • 
  • 

• 飼料中孔雀石綠檢測的固相萃取方法

  飼料中孔雀石綠檢測的固相萃取方法[詳細]

  2024-09-29 02:08

  期刊論文

  |

  • 
  • 
  • 
  • 

• 水產品中孔雀石綠檢測的固相萃取方法

  水產品中孔雀石綠檢測的固相萃取方法[詳細]

  2024-09-30 03:49

  標準

  |

  • 
  • 
  • 
  • 

• LCMS串聯(lián)四極質譜檢測食品中的 孔雀石綠和隱性孔雀石綠

  LCMS串聯(lián)四極質譜檢測食品中的 孔雀石綠和隱性孔雀石綠[詳細]

  2024-09-28 11:33

  選購指南

  |

  • 
  • 
  • 
  • 

• 水產品中孔雀石綠和無色孔雀石綠的測定

  水產品中孔雀石綠和無色孔雀石綠的測定[詳細]

  2011-07-06 00:00

  實驗操作

  |

  • 
  • 
  • 
  • 

• 孔雀石綠試劑盒（水樣）

  孔雀石綠試劑盒（水樣）[詳細]

  2024-10-01 12:16

  報價單

  |

  • 
  • 
  • 
  • 

• 水產品中孔雀石綠檢測的固相萃取方法-ALA-PRS

  水產品中孔雀石綠檢測的固相萃取方法-ALA-PRS[詳細]

  2024-09-28 06:57

  產品樣冊

  |

  • 
  • 
  • 
  • 

• 水產品中孔雀石綠檢測的固相萃取方法-ALN

  水產品中孔雀石綠檢測的固相萃取方法-ALN[詳細]

  2024-09-19 12:49

  標準

  |

  • 
  • 
  • 
  • 

• 水產品中孔雀石綠殘留檢測方法的探討

  水產品中孔雀石綠殘留檢測方法的探討[詳細]

  2024-09-29 12:27

  標準

  |

  • 
  • 
  • 
  • 

• 三聚氰胺快速檢測卡

  本產品為三聚氰胺膠體金快速檢測卡，用于定性、半定量檢測樣品中三聚氰胺殘留，整個檢測過程只需要3~5分鐘左右，液態(tài)奶檢測限0.5mg/kg，嬰兒奶粉檢測限1mg/kg，非嬰兒奶粉檢測限2mg/kg，飼料檢測限為1mg/kg。[詳細]

  2018-08-19 10:00

  產品樣冊

  |

  • 
  • 
  • 
  • 

• 組織因子(TF)檢測試劑盒

  組織因子(TF)檢測試劑盒[詳細]

  2015-04-22 00:00

  期刊論文

  |

  • 
  • 
  • 
  • 

• 水產品中MCX孔雀石綠殘留檢測的固相萃取方法

  水產品中MCX孔雀石綠殘留檢測的固相萃取方法[詳細]

  2024-09-14 21:11

  標準

  |

  • 
  • 
  • 
  • 

• 孔雀石綠英文說明書.pdf

  malachitegreen（MG）ELISA1UsepurposeThiskitforFeed,aquaticsamples,watersamplesintheMGremainingquantitativedetection.2ExperimentalprincipleThiskitadoptsmethodsincompetitionELISAInmicroplatescoatedwithantigenconjugatedMG,addMGstandardorsamples,FreeMGandpre-coatedonstripsofMGconjugatedantigencompeteagainstMGantibodyconjugates,WithTMBchromogenicsubstrate,thecolorischangedfrombluetoyellowafteraddingstopsolution,enzymestandardinstrument450nmwavelengthsintesting,absorblightvalueandinthesamplewasMGcontentisinverselyproportionaltothestandardcurve,throughcalculationsampleswasMGconcentrations.3Materialsprovidedwiththekit3.1Microelisastripplate:1block（12well×8strips）.3.2MGstandard:sixvialsof（1ml/vial）,contentisrespectively:0PPB,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB.3.3Anti-MGantibodyconjugate:1vial（6ml）.3.4ChromogenSolutionA:1vial（6ml）.3.5ChromogenSolutionB:1vial（6ml）.3.6StopSolution:1vial（6ml）,2Msulphuricacid.3.7sampledilution:1vial（10x,6ml）,usedforsampledilutedwith.3.8washsolution:1vial（20x,20ml）,usedforwashingboard.3.9Instruction.4Neednotprovidematerials4.1equipment4.4.1wavelength450nmmicroplate.4.1.2shredder.4.1.3LiangTong.4.1.4oscillators.4.1.5funnel.4.1.6WhatmanNo1orequivalentfilterpaper.4.1.7traceremoveliquiddevice.4.2reagent4.2.1thedeionizedwaterordistilledwater.4.2.2methanol.5Storage5.1kitstoredin2~8℃,Don’tfrozen5.2Don’tuseuptheMicroelisastripplate首ldbesealeddryingpreserve6.Precautions6.1pleasereadtheinstructionscarefully,beforeuseingthekit.6.2don'tuseexpiredkit.6.3beforeusingthekit,pleaseletthereagentrecovertoroomtemperature（25+2℃）,theproposalforatleast2hourstotemperature.6.4thestandardcontainMG,payspecialattentionto,theoperation,we首ldbringgloves.6.5stopsolutioniscontainingsulfate,whenusing,preventburnsskinandcorrosionclothing.6.6differentstandardandsamplesuctionheadusedcannotbemixeduse,otherwise,itwillaffecttestresults.6.7differentbatchesofreagentkitnotmix,Differentstandardandsamplesuctionheadshallnotbeusedincombination,otherwise,itwillaffecttheexperimentalresult.6.8dilutedsamplemustusethiskitofsamplediluent,otherwise,itwillaffecttheexperimentalresult6.9mixedreagents首ldavoidblistering.7Workingliquidpreparation7.1Carbendazimstandards:0ppb,0.1PPB,0.3PPBand0.9PPB,2.7PPB,8.1PPB7.2washsolution:1:20withdistilledwater（1+19）diluted.preparation7.3sampledilutions:1:10withdistilledwater（1+9）diluted.preparation7.3ChromogenSolutionreagent:alreadystandby,avoidlightstraightas7.4stopsolution:alreadyterminationaside8Sampleprocessingprogram（sampleinextractionprocess,muststrictlyaccordingtotheoperationoftheextractionprocess首ldbeaccuratedilution,canappearotherwiseresultsareinaccurate,samples首ldbekeptinacoolplacetoavoidlightandfrozenkeep）8.1Smashthesamplestaken10g,add20ml70%methanolsolution8.2powerfuloscillation3minutes8.3WhatmanNo1filterwith8.4Takethe25μltreatmentofthesamplebyadding25μlsampledilutioninthewells（sampledilutionfactorof2）9Enzyme2lindedanalysissteps9.1experimentalguidelines9.1.1experimentbeginspriortoallreagentinboxesoutsidetheroomtemperature（25fullyrecoveredto+2℃）,timeabout2hours.Returntoroomtemperature（25+2℃）againafterremoveStrips,excessporebartosealimmediatelytothe2~8℃dryingpreserveNote:besuretotemperature,otherwisefullyguaranteetheaccuracyandprecisionoftheaffectingdetection.9.1.2afterusepleaseimmediatelyreagentputback2~8℃preservation9.1.3pleasedon'tchangeanalysisprogram9.1.4pleaseuseaccuratetraceremoveliquiddevice9.1.5operationoncestarted,pleasedonotinterruptanyprogram9.1.6ELISAresultsofrepeatabilityofseveredependsonoperatingprocedures,pleasestrictlyaccordingtorequirementsoperation9.1.7toavoidcross-contamination,eachstandardandsamples首ldusedifferentsuctionheadaddsamples9.1.8plussampledomakesuckaheadcontactmicroporousthesolutionorinsidesurface9.2analysissteps9.2.1beforehandnumbered,markB0,standardandthesamplepositionrecommendeddoubleorificedetection9.2.2taketherequiredamountoftheStrips（Stripsdetachable）,willspareattribwattleandsealedimmediatelyputbackagain2~8℃preservation9.2.3sampledilutions（10）,washsolutionx（20x）dilutionintoworkingliquid（distilledwaterordeionizedwaterdilute）In9.2.4B0welljoining50μl0.0ng/mlstandard9.2.5ineachstandardwelljoining50μlstandard9.2.6ineachsamplewelljoining50μlsamplesolution9.2.7Inallwelljoining50μlAnti-MGantibodyconjugate9.2.8gentlysloshingresponseboardforafewseconds.9.337℃warmbath30min（warmbathprocesssometimespatreactionplate,canreducedoubleorificeerror）9.3.1UncoverClosureplatemembrane,discardLiquid,drybyswing,addwashingbuffertoeverywell,stillfor30sthendrain,repeat5times,drybypat..9.4reaction9.4.1washingprocedurecompleted,immediatelywithliquidapparatusineverytracemovemicroporousfirstjoin50μlChromogenSolutionA,add50μlChromogenSolutionB,Slightsloshingresponseboardmakethoroughlyincorporated379.4.2℃warmbath10min9.4.3eachwelljoining50μlStopSolution,blending9.4.4in450nmtestingabsorbency,resultin5mininsideread.10Theresultscalculated10.1quantitativeanalysis10.1.1obtainedbyeachconcentrationstandardsolutionandtheaveragevalueofasamplespectrophotometry（B）dividedbythefirststandard（0standardabsorbencyvalue（B0）multiplyingby1**%,namelypercentageabsorbencyvalues.B-standardsolutionorsamplesolutionofaverageabsorbanceofthevaluesB0-0μg/Lstandardsolutionofaverageabsorbanceofthevalues10.1.2withMGconcentrationsofvaluesfortheXaxis,100centabsorbanceofthevalueoftheYaxis,drawstandardcurve.Accordingtothesamplepercentageabsorbencyvalues,whichgetcorrespondingpointsfromcurve,namelytheabscissadenotesthemulti-goalMGconcentrationonthenumericalcurvature.theagainstseveralnamelytodetermineMGconcentrationC（ppb）10.1.3becausethesampleafterdilutedinadvance,soaccordingtothestandardcurvegainsfromdifferentconcentrationsamplesmustagainmultiplythedilutedtimes.10.2halfquantitatively10.1.1visualhalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesamplesandstandardsubstanceabsorbencyvaluethediscretionofthejudgeiscompared,samplechromavalueislessthanorgreaterthanstandardvalues.10.1.2instrumenthalfquantitativedetermination:first,chooseanappropriatestandardfluidandsampleswithoperation,accordingtothesampleandstandardcolordepthcomparison,judgesamplechromavalueislessthanorgreaterthanstandardvalues.11SpecificityPhysicalcrossreactionMG1**%CrystalViolet:95%Recessivemalachitegreen:0.1%HiddenCrystalViolet:<0.1%12KitparametersThiskitdetectionlimitis0.05PPBB0absorbanceoftheoptimalvalue首ldbegreaterthan1.0Kitabsorbencyboardinsideerrorislessthan8%,boardbetweenerrorislessthan15%.Withthismanualprovidedatissuesampleextractionmethodrecoveryisgreaterthan80%.13AnalysisrestrictionThiskittestingpositiveforsamples首lduseanothermethodsuchasHPLCorGC/MStobeverified.上海恒遠生物科技有限公司，專業(yè)銷售“孔雀石綠試劑盒”品質保證，值得信賴！如果你想了解該產品的詳細信息，歡迎來電來函！[詳細]

  2018-11-15 10:03

  產品樣冊

  |

  • 
  • 
  • 
  • 

• 農藥殘留檢測卡|農藥殘留速測卡產品樣冊

  農藥殘留檢測卡|農藥殘留速測卡產品樣冊[詳細]

  2016-10-19 17:46

  應用文章

  |

  • 
  • 
  • 
  • 

• 農藥殘留檢測卡|農藥殘留速測卡產品樣冊

  農藥殘留檢測卡|農藥殘留速測卡產品樣冊[詳細]

  2016-10-19 17:46

  課件

  |

  • 
  • 
  • 
  • 

• 黃曲霉毒素M1檢測卡

  黃曲霉毒素M1檢測卡[詳細]

  2012-08-24 00:00

  標準

  |

  • 
  • 
  • 
  •