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資料庫(kù)>Effect of Polyelectrolyte Structure on Protein-Polyelectrolyte Coacervates: Coacervates of Bovine Se
Effect of Polyelectrolyte Structure on Protein-Polyelectrolyte Coacervates: Coacervates of Bovine Se
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2018-08-31 10:11 417閱讀次數(shù)
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Electrostaticinteractionsbetweensyntheticpolyelectrolytesandproteinscanleadtotheformationofdense,macroion-richliquidphases,withequilibriummicroheterogeneitiesonlengthscalesuptohundredsofnanometers.TheeffectsofpHandionicstrengthontherheologicalandopticalpropertiesofthesecoacervatesindicatemicrostructuressensitivetoprotein-polyelectrolyteinteractions.Wereporthereonthepropertiesofcoacervatesobtainedforbovineserumalbumin(BSA)withthebiopolyelectrolytechitosanandfindremarkabledifferencesrelativetocoacervatesobtainedforBSAwithpoly(diallyldimethylammoniumchloride)(PDADMAC).CoacervationwithchitosanoccursmorereadilythanwithPDADMAC.Viscositiesofcoacervatesobtainedwithchitosanaremorethananorderofmagnitudelargerand,unlikethosewithPDADMAC,showtemperatureandshearratedependence.Forthecoacervateswithchitosan,afastrelaxationtimeindynamiclightscattering,attributabletorelativelyunrestrictedproteindiffusioninbothsystems,isdiminishedinintensitybyafactorof3-4,andtheconsequentdominancebyslowmodesisaccompaniedbyamoreheterogeneousarrayofslowapparentdiffusivities.Inplaceofasmall-angleneutronscatteringGuinierregioninthevicinityof0.004-1,a10-foldincreaseinscatteringintensityisobservedatlowerq.Takentogether,theseresultsconfirmthepresenceofdensedomainsonlengthscalesofhundredsofnanometerstomicrometers,whichincoacervatespreparedwithchitosanarelesssolidlike,moreinterconnected,andoccupyalargervolumefraction.Thedifferencesinpropertiesarethuscorrelatedwithdifferencesinmesophasestructure.
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Effect of Polyelectrolyte Structure on Protein-Polyelectrolyte Coacervates: Coacervates of Bovine Se- Electrostaticinteractionsbetweensyntheticpolyelectrolytesandproteinscanleadtotheformationofdense,macroion-richliquidphases,withequilibriummicroheterogeneitiesonlengthscalesuptohundredsofnanometers.TheeffectsofpHandionicstrengthontherheologicalandopticalpropertiesofthesecoacervatesindicatemicrostructuressensitivetoprotein-polyelectrolyteinteractions.Wereporthereonthepropertiesofcoacervatesobtainedforbovineserumalbumin(BSA)withthebiopolyelectrolytechitosanandfindremarkabledifferencesrelativetocoacervatesobtainedforBSAwithpoly(diallyldimethylammoniumchloride)(PDADMAC).CoacervationwithchitosanoccursmorereadilythanwithPDADMAC.Viscositiesofcoacervatesobtainedwithchitosanaremorethananorderofmagnitudelargerand,unlikethosewithPDADMAC,showtemperatureandshearratedependence.Forthecoacervateswithchitosan,afastrelaxationtimeindynamiclightscattering,attributabletorelativelyunrestrictedproteindiffusioninbothsystems,isdiminishedinintensitybyafactorof3-4,andtheconsequentdominancebyslowmodesisaccompaniedbyamoreheterogeneousarrayofslowapparentdiffusivities.Inplaceofasmall-angleneutronscatteringGuinierregioninthevicinityof0.004-1,a10-foldincreaseinscatteringintensityisobservedatlowerq.Takentogether,theseresultsconfirmthepresenceofdensedomainsonlengthscalesofhundredsofnanometerstomicrometers,whichincoacervatespreparedwithchitosanarelesssolidlike,moreinterconnected,andoccupyalargervolumefraction.Thedifferencesinpropertiesarethuscorrelatedwithdifferencesinmesophasestructure.[詳細(xì)]
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2018-08-31 10:11
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