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資料庫(kù)>Cytotoxicity of aluminum oxide nanoparticles on Allium cepa root tipeffects of oxidative stress gen
Cytotoxicity of aluminum oxide nanoparticles on Allium cepa root tipeffects of oxidative stress gen
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2018-08-31 10:11 322閱讀次數(shù)
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ThecommercialusageofAl2O3nanoparticles(Al2O3NPs)hasgoneupsignificantlyintherecenttimes,enhancingtheriskofenvironmentalcontaminationwiththeseagentsandtheirconsequentadverseeffectsonlivingsystems.ThecurrentstudyhasbeendesignedtoevaluatethecytogeneticpotentialofAl2O3NPsinAlliumcepa(roottipcells)atarangeofexposureconcentrations(0.01,0.1,1,10,and100μg/mL),theiruptake/internalizationprofile,andtheoxidativestressgenerated.Wenotedadose-dependentdecreaseinthemitoticindex(42to28%)andanincreaseinthenumberofchromosomalaberrations.Variouschromosomalaberrations,e.g.sticky,multipolarandlaggardchromosomes,chromosomalbreaks,andtheformationofbinucleatecells,wereobservedbyoptical,fluorescence,andconfocallaserscanningmicroscopy.FT-IRanalysisdemonstratedthesurfacechemicalinteractionbetweenthenanoparticlesandroottipcells.ThebiouptakeofAl2O3inparticulateformledtoreactiveoxygenspeciesgeneration,whichinturnprobablycontributedtotheinductionofchromosomalaberrations.
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Cytotoxicity of aluminum oxide nanoparticles on Allium cepa root tipeffects of oxidative stress gen- ThecommercialusageofAl2O3nanoparticles(Al2O3NPs)hasgoneupsignificantlyintherecenttimes,enhancingtheriskofenvironmentalcontaminationwiththeseagentsandtheirconsequentadverseeffectsonlivingsystems.ThecurrentstudyhasbeendesignedtoevaluatethecytogeneticpotentialofAl2O3NPsinAlliumcepa(roottipcells)atarangeofexposureconcentrations(0.01,0.1,1,10,and100μg/mL),theiruptake/internalizationprofile,andtheoxidativestressgenerated.Wenotedadose-dependentdecreaseinthemitoticindex(42to28%)andanincreaseinthenumberofchromosomalaberrations.Variouschromosomalaberrations,e.g.sticky,multipolarandlaggardchromosomes,chromosomalbreaks,andtheformationofbinucleatecells,wereobservedbyoptical,fluorescence,andconfocallaserscanningmicroscopy.FT-IRanalysisdemonstratedthesurfacechemicalinteractionbetweenthenanoparticlesandroottipcells.ThebiouptakeofAl2O3inparticulateformledtoreactiveoxygenspeciesgeneration,whichinturnprobablycontributedtotheinductionofchromosomalaberrations.[詳細(xì)]
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2018-08-31 10:11
產(chǎn)品樣冊(cè)
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- Cytotoxicity of aluminum oxide nanoparticles on Allium cepa root tip—effects of oxidative stress generation and biouptake
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