For decades, cryopreservation has been used for plant cells, unfertilized (oocytes)/fertilized (zygotes) human and animal eggs, and male sperm cells. The cryopreservation process typically involves using liquid nitrogen to quickly deep freeze the cells to -196°C allowing all metabolic processes to stop, preserving them for storage, shipping, or future use.When thawed, the cells resume their normal metabolic processes. 數(shù)十年來，冷凍法一直被人們用來保存細(xì)胞、卵子、受精卵、精子等。冷凍過程是用液氮將細(xì)胞快速冷卻到-196度以便使細(xì)胞停止代謝，這樣就可以方便地進行儲存，運輸以備將來使用。一旦解凍，細(xì)胞恢復(fù)代謝。