摘要 目的 應(yīng)用GX液相色譜法測定龜芪壯骨顆粒中二苯乙烯苷的含量方法 采用DiamonsilTM(鉆石)C18(5m，250mm×4.6mm)，乙腈－0.2%磷酸溶液（23：77）為流動相，流速：1ml/min，檢測波長為320nm結(jié)果 線性范圍為0.0964～0.482g，r=0.9998，平均回收率為98.00％，RDS=0.98％結(jié)論 該方法用于測定龜芪壯骨顆粒中二苯乙烯苷含量，簡便快速準(zhǔn)確 關(guān)鍵詞 龜芪壯骨顆粒；二苯乙烯苷；GX液相色譜法 Abstract Objective To develop a HPLC method to determine the content of 2，3，5，4-tetrahydroxystibene-2-o--D-glucoside in Guiqizhuanggu granule.Methods The HPLC method was carried out on an DiamonsilTMC18 column(5m，250mm×4.6mm)using a mobile phase of acetonitrile-0.2% phosphoric acid water(23：77)，and detection wavelength was 320 nm.Results With a relation efficient of 0.9998. Good linear relationshipe between area and a mount was noted for 0.0964～0.482g. The average recovery of 2，3，5，4-tetrahydroxystibene-2-o--D-glucoside and RSD were 98.00% and 0.98% respectively. Conclusion It was proved that the method was available，simple and accurate. Key words guiqizhuanggu granule； 2，3，5，4-tetrahydroxystibene-2-o--D-glucoside； HPLC 龜芪壯骨顆粒是熟地黃龜甲杜仲等十八味中藥組成主要功能為補氣補血，滋補肝腎，強筋壯骨適用于腰膝酸軟，氣血不足等癥方中主要藥物何首烏為蓼科植物何首烏的干燥塊根其有效成分之一為二苯乙烯苷本實驗采用GX液相色譜法［1，2］，測定龜芪壯骨顆粒中二苯乙烯苷的含量作為該制劑的質(zhì)量控制指標(biāo)，以便更好地控制該產(chǎn)品質(zhì)量 1 儀器與試藥 固相圓盤萃取