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資料庫(kù)>Titanium Dioxide Nanoparticles Trigger Loss of Hepatic Function and Perturbation of Mitochondrial Dy
Titanium Dioxide Nanoparticles Trigger Loss of Hepatic Function and Perturbation of Mitochondrial Dy
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2018-08-31 10:11 618閱讀次數(shù)
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Titaniumdioxide(TiO2)nanoparticlesareoneofthemosthighlymanufacturednanomaterialsintheworldwithapplicationsincopiousindustrialandconsumerproducts.Theliverisamajoraccumulationsiteformanynanoparticles,includingTiO2,directlythroughintentiona領(lǐng)estionorindirectlythroughincreasedenvironmentalcontaminationandunintentiona領(lǐng)estionviawater,foodoranimals.GrowingconcernsoverthecurrentusageofTiO2coupledwiththelackofmechanisticunderstandingofitspotentialhealthriskisthemotivationforthisstudy.HerewedeterminedthetoxiceffectofthreedifferentTiO2nanoparticles(commerciallyavailablerutile,anataseandP25)onprimaryrathepatocytes.Specifically,weevaluatedeventsrelatedtohepaticfunctionsandmitochondrialdynamics:(1)ureaandalbuminsynthesisusingcolorimetricandELISAassays,respectively;(2)redoxsigna領(lǐng)mechanismsbymeasuringROSproduction;(3)OPA1andMfn-1expressionthatmediatesthemitochondriadynamicsbyPCR;and(4)mitochondrialmorphologybyMitoTrackerGreenFMstaining.AllthreeTiO2nanoparticlesinducedasignificantlossinhepaticfunctionsevenatconcentrationsaslowas20μg/mlwithcommerciallyusedP25causingmaximumdamage.TiO2nanoparticlesinducedastrongoxidativestressinprimaryhepatocytes.TiO2nanoparticlesexposurealsoresultedinmorphologicalchangesinmitochondriaandsignificantlossinthefusionprocess,thusimpairingthemitochondrialdynamics.AlthoughthisstudydemonstratedthatTiO2nanoparticlesexposureresultedinsignificantdamageinprimaryhepatocytes,moreinvitroandinvivostudiesarerequiredtodeterminethecompletetoxicologicalmechanismonprimaryhepatocytesandsubsequentlyliverfunction.
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Titanium Dioxide Nanoparticles Trigger Loss of Hepatic Function and Perturbation of Mitochondrial Dy- Titaniumdioxide(TiO2)nanoparticlesareoneofthemosthighlymanufacturednanomaterialsintheworldwithapplicationsincopiousindustrialandconsumerproducts.Theliverisamajoraccumulationsiteformanynanoparticles,includingTiO2,directlythroughintentiona領(lǐng)estionorindirectlythroughincreasedenvironmentalcontaminationandunintentiona領(lǐng)estionviawater,foodoranimals.GrowingconcernsoverthecurrentusageofTiO2coupledwiththelackofmechanisticunderstandingofitspotentialhealthriskisthemotivationforthisstudy.HerewedeterminedthetoxiceffectofthreedifferentTiO2nanoparticles(commerciallyavailablerutile,anataseandP25)onprimaryrathepatocytes.Specifically,weevaluatedeventsrelatedtohepaticfunctionsandmitochondrialdynamics:(1)ureaandalbuminsynthesisusingcolorimetricandELISAassays,respectively;(2)redoxsigna領(lǐng)mechanismsbymeasuringROSproduction;(3)OPA1andMfn-1expressionthatmediatesthemitochondriadynamicsbyPCR;and(4)mitochondrialmorphologybyMitoTrackerGreenFMstaining.AllthreeTiO2nanoparticlesinducedasignificantlossinhepaticfunctionsevenatconcentrationsaslowas20μg/mlwithcommerciallyusedP25causingmaximumdamage.TiO2nanoparticlesinducedastrongoxidativestressinprimaryhepatocytes.TiO2nanoparticlesexposurealsoresultedinmorphologicalchangesinmitochondriaandsignificantlossinthefusionprocess,thusimpairingthemitochondrialdynamics.AlthoughthisstudydemonstratedthatTiO2nanoparticlesexposureresultedinsignificantdamageinprimaryhepatocytes,moreinvitroandinvivostudiesarerequiredtodeterminethecompletetoxicologicalmechanismonprimaryhepatocytesandsubsequentlyliverfunction.[詳細(xì)]
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2018-08-31 10:11
產(chǎn)品樣冊(cè)
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- Titanium Dioxide Nanoparticles Trigger Loss of Hepatic Function and Perturbation of Mitochondrial Dynamics in Primary Hepatocytes
- Titanium Dioxide Nanoparticles Trigger Loss of Hepatic Function and Perturbation of Mitochondrial Dynamics in Primary Hepatocytes[詳細(xì)]
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2024-09-28 00:49
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