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姜黃素大鼠炎癥實驗灌胃

本文由 上海易佰聚經(jīng)貿(mào)有限公司 整理匯編

2018-09-02 10:00 1392閱讀次數(shù)

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Acrylonitrile(AN)iswidelyusedinthemanufacturingoffibers,plasticsandpharmaceuticals.FreeradicalmediatedlipidperoxidationisimplicatedinthetoxicityofAN.Thepresentstudywasdesignedtoexaminetheabilityofcurcumin,anaturalpolyphenoliccompound,toattenuateacuteAN-inducedlipidperoxidationinthebrainandliverofrats.MaleSpragueDawleyratswereorallyadministeredcurcuminatdosesof0(oliveoilcontrol),50or100mg/kgbodyweightdailyfor7consecutivedays.Twohoursafterthelastdoseofcurcumin,ratsreceivedanintraperitonealinjectionof50mgAN/kgbodyweight.AcuteexposuretoANsignificantlyincreasedthegenerationoflipidperoxidationproducts,reflectedbyhighlevelsofmalondialdehyde(MDA)bothinthebrainandliver.Theseincreaseswereaccompaniedbyasignificantdecreaseinreducedglutathione(GSH)contentandasignificantreductionincatalase(CAT)activityinthesametissues.Noconsistentchangesinsuperoxidedismutase(SOD)activitywereobservedbetweenthecontrolandAN-treatmentgroupsinbothtissues.PretreatmentwithcurcuminreversedtheAN-inducedeffects,reducingthelevelsofMDAandenhancingCATactivityandincreasingreducedGSHcontentbothinthebrainandliver.Furthermore,curcumineffectivelypreventedAN-induceddecreaseincytochromecoxidaseactivityinbothliverandbrain.TheseresultsestablishthatcurcuminpretreatmenthasabeneficialroleinmitigatingAN-inducedoxidativestressbothinthebrainsandliversofexposedratsandtheseeffectsaremediatedindependentlyofcytochromeP4502E1inhibition.Accordingly,curcumin首ldbeconsideredasapotentialsafeandeffectiveapproachinattenuatingtheadverseeffectsproducedbyAN-relatedtoxicants.佰易聚生物商城提供優(yōu)質(zhì)的分子生物學試劑和試劑盒、美國聯(lián)合碳化和美國光譜醫(yī)學透析袋、培養(yǎng)基和培養(yǎng)耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆促銷中,歡迎登錄www.ebioeasy.com或者撥打021-61805605,61805606,61805610洽談選購!

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