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pEF1/myc-His C載體說(shuō)明書(shū)
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型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPI0028pEF1/myc-HisCInvitrogen哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：EF-1αVectorType：pEFCloningMethod：RestrictionEnzyme/MCSProteinTagorFusion：c-MycEpitopeTag，HisTag（6xHis）ConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:pEF1/myc-HisA,B,andCare6.2kbvectorsderivedfrompcDNA3.1/myc-Hisanddesignedforoverproductionofrecombinantproteinsinmammaliancelllines.Featuresofthevectorsallowpurificationanddetectionofexpressedproteins.High-levelstableandtransientexpressioncanbecarriedoutinmostmammaliancells.Thevectorscontainthefollowingelements:Humanelongationfactor1?-subunit（hEF-1α）promoterforhigh-levelexpressionacrossabroadrangeofspeciesandcelltypes（Goldmanetal.,1996;MizushimaandNagata,1990）.Threereadingframestofacilitatein-framecloningwithaC-terminaltagencodingthemyc（c-myc）epitopeandapolyhistidinemetal-bindingpeptide.Neomycinresistancegeneforselectionofstablecelllines.EpisomalreplicationincelllinesthatarelatentlyinfectedwithSV40orthatexpresstheSV40largeTantigen（e.g.,COS7）.Thecontrolplasmid,pEF1/myc-His/lacZ,isincludedforuseasapositivecontrolfortransfection,expression,anddetectioninthecelllineofchoice.質(zhì)粒圖譜:
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pEF1/myc-His C載體說(shuō)明書(shū)- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPI0028pEF1/myc-HisCInvitrogen哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：EF-1αVectorType：pEFCloningMethod：RestrictionEnzyme/MCSProteinTagorFusion：c-MycEpitopeTag，HisTag（6xHis）ConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:pEF1/myc-HisA,B,andCare6.2kbvectorsderivedfrompcDNA3.1/myc-Hisanddesignedforoverproductionofrecombinantproteinsinmammaliancelllines.Featuresofthevectorsallowpurificationanddetectionofexpressedproteins.High-levelstableandtransientexpressioncanbecarriedoutinmostmammaliancells.Thevectorscontainthefollowingelements:Humanelongationfactor1?-subunit（hEF-1α）promoterforhigh-levelexpressionacrossabroadrangeofspeciesandcelltypes（Goldmanetal.,1996;MizushimaandNagata,1990）.Threereadingframestofacilitatein-framecloningwithaC-terminaltagencodingthemyc（c-myc）epitopeandapolyhistidinemetal-bindingpeptide.Neomycinresistancegeneforselectionofstablecelllines.EpisomalreplicationincelllinesthatarelatentlyinfectedwithSV40orthatexpresstheSV40largeTantigen（e.g.,COS7）.Thecontrolplasmid,pEF1/myc-His/lacZ,isincludedforuseasapositivecontrolfortransfection,expression,anddetectioninthecelllineofchoice.質(zhì)粒圖譜:[詳細(xì)]
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2024-10-05 18:26
產(chǎn)品樣冊(cè)
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- pEF1/myc-His A載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPI0026pEF1/myc-HisAInvitrogen哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：EF-1αVectorType：pEFCloningMethod：RestrictionEnzyme/MCSProteinTagorFusion：c-MycEpitopeTag，HisTag（6xHis）ConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:pEF1/myc-HisA,B,andCare6.2kbvectorsderivedfrompcDNA3.1/myc-Hisanddesignedforoverproductionofrecombinantproteinsinmammaliancelllines.Featuresofthevectorsallowpurificationanddetectionofexpressedproteins.High-levelstableandtransientexpressioncanbecarriedoutinmostmammaliancells.Thevectorscontainthefollowingelements:Humanelongationfactor1?-subunit（hEF-1α）promoterforhigh-levelexpressionacrossabroadrangeofspeciesandcelltypes（Goldmanetal.,1996;MizushimaandNagata,1990）.Threereadingframestofacilitatein-framecloningwithaC-terminaltagencodingthemyc（c-myc）epitopeandapolyhistidinemetal-bindingpeptide.Neomycinresistancegeneforselectionofstablecelllines.EpisomalreplicationincelllinesthatarelatentlyinfectedwithSV40orthatexpresstheSV40largeTantigen（e.g.,COS7）.Thecontrolplasmid,pEF1/myc-His/lacZ,isincludedforuseasapositivecontrolfortransfection,expression,anddetectioninthecelllineofchoice.質(zhì)粒圖譜:[詳細(xì)]
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2024-10-03 09:21
產(chǎn)品樣冊(cè)
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- pEF1/myc-His B載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPI0027pEF1/myc-HisBInvitrogen哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：EF-1αVectorType：pEFCloningMethod：RestrictionEnzyme/MCSProteinTagorFusion：c-MycEpitopeTag，HisTag（6xHis）ConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:pEF1/myc-HisA,B,andCare6.2kbvectorsderivedfrompcDNA3.1/myc-Hisanddesignedforoverproductionofrecombinantproteinsinmammaliancelllines.Featuresofthevectorsallowpurificationanddetectionofexpressedproteins.High-levelstableandtransientexpressioncanbecarriedoutinmostmammaliancells.Thevectorscontainthefollowingelements:Humanelongationfactor1?-subunit（hEF-1α）promoterforhigh-levelexpressionacrossabroadrangeofspeciesandcelltypes（Goldmanetal.,1996;MizushimaandNagata,1990）.Threereadingframestofacilitatein-framecloningwithaC-terminaltagencodingthemyc（c-myc）epitopeandapolyhistidinemetal-bindingpeptide.Neomycinresistancegeneforselectionofstablecelllines.EpisomalreplicationincelllinesthatarelatentlyinfectedwithSV40orthatexpresstheSV40largeTantigen（e.g.,COS7）.Thecontrolplasmid,pEF1/myc-His/lacZ,isincludedforuseasapositivecontrolfortransfection,expression,anddetectioninthecelllineofchoice.質(zhì)粒圖譜:[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pPICZα C載體說(shuō)明書(shū)
- pPICZαCOX1promoterregion:bases1-941OX1primingsite:bases855-875α-factorsignalsequence:bases941-1207Multiplecloningsite:bases1208-1276c-mycepitope:bases1275-1304Polyhistidine（6xHis）tag:bases1320-13373AOX1primingsite:bases1423-1443AOX1transcriptionterminationregion:bases1341-1682TEF1promoter:bases1683-2093EM7promoter:bases2095-2162ShbleORF:bases2163-2537CYC1transcriptionterminationregion:bases2538-2855pUCorigin:bases2866-3539（complementarystrand）質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pGAPZα C載體說(shuō)明書(shū)
- pGAPZαC型號(hào)載體名稱(chēng)出品公司載體用途VJI0295pGAPZαCInvitrogen酵母表達(dá)載體Theglyceraldehyde-3-phosphatedehydrogenase（GAPDH）enzymeisconstitutivelyexpressedathighlevelsinmanyorganisms,includingPichiapastoris.Thepromoterofthegene（GAP）encodingtheGAPDHproteinhasrecentlybeencharacterizedandshowntoexpressrecombinantproteinstohighlevelsinPichiapastoris,dependingonthecarbonsourceused（Waterhametal.,1997）.ThelevelofexpressionseenwiththeGAPpromoter（PGAP）canbeslightlyhigherthanthatobtainedwiththeAOX1promoter.ThepGAPZA,B,andCvectors（2.9kb）andpGAPZαA,B,andC（3.1kb）vectorsusetheGAPpromotertoconstitutivelyexpressrecombinantproteinsinPichiapastoris.ProteinscanbeexpressedasfusionstoaC-terminalpeptidecontainingthemycepitopefordetectionandapolyhistidinetagforpurificationonmetal-chelatingresin（i.e.ProBond）.Inaddition,pGAPZαproducesproteinsfusedtoanN-terminalpeptideencodingtheSaccharomycescerevisiaeα-factorsecretionsignal.BothvectorsaresuppliedinthreereadingframestofacilitateinframecloningwiththeC-terminaltagand/ortheN-terminalsecretionsignal.Selectionofthesevectorsisbasedonthedominantselectablemarker,Zeocin,whichisbifunctionalinbothPichiaandE.coli.質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pcDNA3.1（-）/myc-His A&B&C說(shuō)明書(shū)
- pcDNA3.1（-）/myc-HisA&B&C型號(hào)載體名稱(chēng)出品公司載體用途VPI0017pcDNA3.1（-）/myc-HisAInvitrogen哺乳動(dòng)物表達(dá)載體VPI0018pcDNA3.1（-）/myc-HisBInvitrogen哺乳動(dòng)物表達(dá)載體VPI0019pcDNA3.1（-）/myc-HisCInvitrogen哺乳動(dòng)物表達(dá)載體產(chǎn)品參數(shù):MammalianSelection：G418，neoProteinTagorFusion：c-MycEpitopeTag，HisTag（6xHis）SequencingPrimer：T7FwdSequencingPrimerSequence：5'd[TAATACGACTCACTATAGGG]3'載體抗性:氨芐青霉素（Ampicillin）質(zhì)粒圖譜:[詳細(xì)]
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2024-09-29 02:42
產(chǎn)品樣冊(cè)
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- pIRES-neo載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPC0046pIRES-neoClontech哺乳動(dòng)物表達(dá)載體800VPC0047pIRES-neo2Clontech哺乳動(dòng)物表達(dá)載體800VPC0048pIRES-neo3Clontech哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：CMVCloningMethod：RestrictionEnzyme/MCSConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:pIRES-neo、pIRES-neo2、pIRES-neo3三者在主體結(jié)構(gòu)上差別不大，主要差別在于MCS。具體情況請(qǐng)參見(jiàn)質(zhì)粒圖譜。質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pIRES-neo3載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPC0046pIRES-neoClontech哺乳動(dòng)物表達(dá)載體800VPC0047pIRES-neo2Clontech哺乳動(dòng)物表達(dá)載體800VPC0048pIRES-neo3Clontech哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：CMVCloningMethod：RestrictionEnzyme/MCSConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:pIRES-neo、pIRES-neo2、pIRES-neo3三者在主體結(jié)構(gòu)上差別不大，主要差別在于MCS。具體情況請(qǐng)參見(jiàn)質(zhì)粒圖譜。質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pIRES-neo2 載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPC0046pIRES-neoClontech哺乳動(dòng)物表達(dá)載體800VPC0047pIRES-neo2Clontech哺乳動(dòng)物表達(dá)載體800VPC0048pIRES-neo3Clontech哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：CMVCloningMethod：RestrictionEnzyme/MCSConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:pIRES-neo、pIRES-neo2、pIRES-neo3三者在主體結(jié)構(gòu)上差別不大，主要差別在于MCS。具體情況請(qǐng)參見(jiàn)質(zhì)粒圖譜。質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pIRES載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPC0045pIRESClontech哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionMammalianSelection：Neo，G418Promoter：CMVCloningMethod：RestrictionEnzyme/MCSConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:由于IRES元件的存在，pIRES可以同時(shí)表達(dá)兩個(gè)基因。兩個(gè)基因在轉(zhuǎn)錄水平上基本一致，但是在翻譯水平上差異較大。有數(shù)據(jù)稱(chēng)，IRES后面的基因，表達(dá)量約只有IRES前面的基因的二分之一甚至三分之一。很多研究者在將pIRES2-EGFP轉(zhuǎn)染至細(xì)胞后，會(huì)發(fā)現(xiàn)綠色熒光很少很弱，其中一個(gè)原因就是IRES元件的特性所致。因此，如果您要同時(shí)表達(dá)兩個(gè)地位相等的基因，那么還是建議您采用雙表達(dá)框載體，例如Invitrogen的pBudCE4.1、Invivogen的pVitro2-neo-mcs、Yrbio的pYr-adshuttle-3等。這些載體上都有兩個(gè)外源基因表達(dá)框，分別由兩個(gè)不同的強(qiáng)啟動(dòng)子驅(qū)動(dòng)，可以實(shí)現(xiàn)兩個(gè)基因的同時(shí)表達(dá)，且表達(dá)強(qiáng)度也非?？捎^。質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pCMV-Myc載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPC0050pCMV-MycClontech哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionPromoter：CMVCloningMethod：RestrictionEnzyme/MCSConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）載體描述:ThepCMV-MycMammalianExpressionVectorexpressesproteinscontainingtheN-terminalc-Mycepitopetag.Thec-Mycepitopetagiswell-characterizedandhighlyimmunoreactive.High-levelexpressioninmammaliancellsisdrivenfromthehumancytomegalovirusimmediateearlypromoter/enhancer（P/CMVIE）.Thevectorcontainsanintron（splicedonor/spliceacceptor）;theepitopetag;anCMVIEMCS;andapolyadenylationsignalfromSV40.ThisvectoralsopossessestheampicillinresistancegeneforselectioninE.coli.質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pSI載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPP0044pSIPromega哺乳動(dòng)物表達(dá)載體800載體描述:ThepSIMammalianExpressionVectorpromotesconstitutiveexpressionofclonedDNAinsertsinmammaliancells.ThemajordifferencebetweenthepCIandpSIMammalianExpressionVectorsistheenhancer/promoterregioncontrol領(lǐng)theexpressionoftheinsertedgene.ThepSIExpressionVectorcontainsthesimianvirus40（SV40）enhancerandearlypromoterregion.Thisvectorcanbeusedforbothtransientandstableexpressionofgenes.Forstableexpression,thepSIVectormustbeco-transfectedwithanexpressionvectorcontainingaselectablegeneformammaliancells.質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pCI載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPP0043pCIPromega哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):Promotor:CMVSize:40065'Sequencing1PrimerSequence:T7載體抗性:氨芐青霉素（Ampicillin）載體描述:ThepCIMammalianExpressionVectorpromotesconstitutiveexpressionofclonedDNAinsertsinmammaliancells.ThemajordifferencebetweenthepCIandpSIMammalianExpressionVectorsistheenhancer/promoterregioncontrol領(lǐng)theexpressionoftheinsertedgene.ThepCIExpressionVectorcontainsthehumancytomegalovirus（CMV）majorimmediate-earlygeneenhancer/promoterregion.Thisvectorcanbeusedforbothtransientandstableexpressionofgenes.Forstableexpression,thepCIVectormustbeco-transfectedwithanexpressionvectorcontainingaselectablegeneformammaliancells.質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pCMV-HA載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VPC0051pCMV-HAClontech哺乳動(dòng)物表達(dá)載體800產(chǎn)品參數(shù):KeyFunction：ConstitutiveExpressionPromoter：CMVCloningMethod：RestrictionEnzyme/MCSConstitutiveorInducibleSystem：Constitutive載體抗性:氨芐青霉素（Ampicillin）質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pIRES-hrGFP-1a載體說(shuō)明書(shū)
- 型號(hào)載體名稱(chēng)出品公司載體用途價(jià)格VYC0107pIRES-hrGFP-1aClontech熒光蛋白報(bào)告載體800VitalityhrGFPMammalianExpressionVectorspIRES-hrGFP-1a,pIRES-hrGFP-2a,phrGFP-C,phrGFP-N1,phrGFP-1,andphrGFPMATERIALSPROVIDEDMaterialprovidedConcentrationQuantitypIRES-hrGFP-1a（Catalog#240031）1.0g/l20gpIRES-hrGFP-2a（Catalog#240032）1.0g/l20gphrGFP-C（Catalog#240035）1.0g/l20gphrGFP-N1（Catalog#240036）1.0g/l20gphrGFP-1（Catalog#240059）1.0g/l20gphrGFP（Catalog#240062）1.0g/l20g質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
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- pBudCE4.1載體說(shuō)明書(shū)
- pBudCE4.1型號(hào)載體名稱(chēng)出品公司載體用途VPI0014pBudCE4.1Invitrogen哺乳動(dòng)物表達(dá)載體產(chǎn)品參數(shù):MammalianSelection：ZeocinSequencingPrimer：CMV-f、hEF1α-fTag：6XHis，myc載體抗性:博萊霉素（Zeocin）載體描述:質(zhì)粒特點(diǎn)：雙表達(dá)框，兩個(gè)強(qiáng)啟動(dòng)子（CMV啟動(dòng)子和hEF1α啟動(dòng)子）各負(fù)責(zé)一個(gè)，不會(huì)互相干擾，從而可以實(shí)現(xiàn)同時(shí)表達(dá)兩個(gè)基因，且兩者表達(dá)不互相影響的目的。如果在hEF1α啟動(dòng)子后面插入EGFP或者其它熒光基因，CMV啟動(dòng)子后插入外源基因，則可以做到既有熒光蛋白來(lái)做轉(zhuǎn)染指示，且熒光基因表達(dá)和外源基因表達(dá)又不會(huì)互相干擾。Yrbio已經(jīng)構(gòu)建了pBudCE4.1-EGFP、pBudCE4.1-RFP、pBudCE4.1-YFP等質(zhì)粒，客戶(hù)可直接購(gòu)買(mǎi)。質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pVAX1載體說(shuō)明書(shū)
- pVAX1型號(hào)載體名稱(chēng)出品公司載體用途VPI0038pVAX1Invitrogen哺乳動(dòng)物表達(dá)載體產(chǎn)品參數(shù):MammalianSelection：-SequencingPrimer：T7FwdSequencingPrimerSequence：5'd[TAATACGACTCACTATAGGG]3'載體抗性:卡那霉素（Kanamycin）載體描述:pVAX1是在載體pcDNA3.1的基礎(chǔ)上改建而成的一種新型真核表達(dá)載體，大小為3.0kb。該質(zhì)粒用卡那霉素抗性篩選基因替代pcDNA3.1中的氨卞霉素抗性篩選基因，Z*程度地減少抗性篩選基因和人類(lèi)基因組發(fā)生重組的可能性。pVAX1含有多個(gè)克隆酶切位點(diǎn)允許同時(shí)克隆多個(gè)大片段的目的基因，強(qiáng)啟動(dòng)子pCMV和BGHpolyA信號(hào)可以在哺乳動(dòng)物細(xì)胞中高水平表達(dá)重組蛋白。pVAX1又是一種非融合載體，要求插入序列中時(shí)必須包含一段KozaK的翻譯起始序列和一個(gè)起始密碼子（ATG）以引導(dǎo)正確的翻譯，典型的KozaK共有序列為“ANNATGG”，其-3位的A和+4位的G對(duì)正確插入甚為關(guān)鍵，在插入序列中還需包括一個(gè)終止密碼子以正確終止基因表達(dá)。pVAX1是由美國(guó)食品和藥品管理委員會(huì)（FDA）推薦的**可以應(yīng)用于人體實(shí)驗(yàn)的載體質(zhì)粒。迄今為止，關(guān)于pVAX1的研究已涉及動(dòng)物和臨床試驗(yàn)。質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
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- pBC1載體說(shuō)明書(shū)
- pBC1型號(hào)載體名稱(chēng)出品公司載體用途VCI0066pBC1Invitrogen重組蛋白乳汁表達(dá)系統(tǒng)GeneralDescriptionDNApBC1Entiremoleculelength:21628bp質(zhì)粒圖譜:[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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- pDisplay載體說(shuō)明書(shū)
- pDisplay型號(hào)載體名稱(chēng)出品公司載體用途VBI0471pDisplayInvitrogen哺乳動(dòng)物表面展示系統(tǒng)產(chǎn)品參數(shù):MammalianSelection：Neomycin，G418Host：mammalianN/CTerm：CterminusMCS：SfiI-BglII-XmaI-SmaI-SacII-PstI-SalI-AccI載體抗性:氨芐青霉素（Ampicillin）載體描述:pDisplayisamammalianexpressionvectordesignedtotargetrecombinantproteinstothesurfaceofmammaliancells.Displayedproteinscanbeanalyzedfortheirabilitytointeractwithknownorputativeligands.ProteinsofinterestaretargetedandanchoredtothecellsurfacebycloningthegeneofinterestinframewiththevectorsuniqueN-terminalsecretionsignalandtheC-terminaltransmembraneanchoringdomainofplatelet-derivedgrowthfactorreceptor（PDGFR）.Incontrasttophagedisplayvectorswhichoperateexclusivelyinprokaryoticcells,thepDisplayVectorofferstheadvantageofhavingthedisplayedproteinofinterestprocessedinmammaliancells.Therefore,recombinantproteinsofeukaryoticoriginthatareexpressedfrompDisplaymorecloselyresembletheirnativeform.Thismayfavormoreaccurateligandbindinginteractions.InadditiontotheN-terminalcellsurfacetargetingsignalandC-terminaltransmembraneanchoringdomain,thepDisplayVectorcontainsthefollowingkeyfeatures:T7promoter/primingsiteforinvitrotranscriptionofsenseRNAandforsequencingofinsertsNeomycinresistancemarkerforstableselectioninmammaliancellsusingGeneticinSV40originforreplicationandsimplevectorrescueincelllinesexpressingthelargeTantigen（e.g.,COS-1andCOS-7）AmpicillinresistancegeneforselectioninE.coli質(zhì)粒圖譜:上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
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- EBY100載體說(shuō)明書(shū)
- EBY100型號(hào)載體名稱(chēng)出品公司載體用途VNI0473EBY100Invitrogen配套釀酒酵母Contents20gofpYD1,lyophilizedinTE,pH8EBY100glycerolstock,0.5ml2gpYD1Forwardsequencingprimer,lyophilized2gpYD1Reversesequencingprimer,lyophilizedShipping/StorageThepYD1VectorKitisshippedondryice.Uponreceipt:Storetheglycerolstockat-80°CStorethelyophilizedplasmidandprimersat-20°C上海起福生物科技有限公司聯(lián)系人：楊建輝400電話(huà)：4006551678辦公電話(huà)：021-50724187傳真：021-50724961*8006手機(jī)：15921799099企業(yè)QQ:4006551678網(wǎng)址:http://www.qfbio.com/地址：上海市浦東新區(qū)繡川路561號(hào)1102室[詳細(xì)]
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2018-09-29 10:02
產(chǎn)品樣冊(cè)
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