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Study of Protein Hydrodynamics with Light Scatter-ing: Size and Charge of Lysozyme
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2018-08-31 10:11 314閱讀次數(shù)
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Effectivedeterminationofthesizeandchargestatusofbiologicalmacromoleculeslikeproteinsplaysacrucialroleinpharmaceutical,clinicalandotherbiotechnologicalapplications.Inthepresentstudy,dynamiclightscattering(DLS)andelectrophoreticlightscattering(ELS)areemployedtocharacterizethesizeandchargeofLysozyme,acommonprotein.Itisshownthatthesensitivityandprecisionofthesetechniquesarewellsuitabletodiscernthesubtleeffectsofbuffersolutionconditionsontheproteinsizeandcharge.
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Study of Protein Hydrodynamics with Light Scatter-ing: Size and Charge of Lysozyme
- Effectivedeterminationofthesizeandchargestatusofbiologicalmacromoleculeslikeproteinsplaysacrucialroleinpharmaceutical,clinicalandotherbiotechnologicalapplications.Inthepresentstudy,dynamiclightscattering(DLS)andelectrophoreticlightscattering(ELS)areemployedtocharacterizethesizeandchargeofLysozyme,acommonprotein.Itisshownthatthesensitivityandprecisionofthesetechniquesarewellsuitabletodiscernthesubtleeffectsofbuffersolutionconditionsontheproteinsizeandcharge.[詳細(xì)]
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