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  Page1/5MaterialSafetyDataSheetacc.toISO/DIS11014Printingdate08/28/2008Reviewedon08/26/2008DR1Identificationofsubstance:Productname:BDMatrigelBasementMembraneMatrixCatalognumber:354230,354234,354277,356230,356231,356234,356235,356237Manufacturer/Supplier:BDBiosciences-DiscoveryLabware2OakParkBedford,MA01730Tel:877.232.8995Informationdepartment:Forfurtherinformation,pleasecallTechnicalServicesat（877）232-8995Emergencyinformation:Incaseofachemicalemergency,spill,fire,exposure,oraccidentcontactBDDiscoveryLabwareat800.334.5229,orChemTrecat800.424.9300.2Composition/Dataoncomponents:ChemicalcharacterizationDescription:Mixtureofthesubstanceslistedbelowwithnonhazardousadditions.Dangerouscomponents:CASNO.67-66-3Chloroform≤0.02CASNO.Description%chloroformCarc.Cat.3;Xn,Xi;R22-38-40-48/20/22Danger:Flame2.6/2Warning:Healthhazard3.6/2,3.9/2;Exclamationmark3.1.O/4,3.2/23HazardsidentificationHazarddescription:Thisproductcontainsnohazardousconstituents,ortheconcentrationofallchemicalconstituentsarebelowtheregulatorythresholdlimitsdescribedbyOccupationalSafetyHealthAdministrationHazardCommunicationStandard29CFR1910.1200andtheEuropeanDirective91/155/EEC,and93/112/EC.ClassificationsystemTheclassificationwasmadeaccordingtothelatesteditionsofinternationalsubstanceslists,andexpandeduponfromcompanyandliteraturedata.NFPAratings（scale0-4）000Health=0Fire=0Reactivity=04FirstaidmeasuresAfterinhalationSupplyfreshair;consultdoctorincaseofcomplaints.AfterskincontactImmediatelywashwithwaterandsoapandrinsethoroughly.AftereyecontactRinseopenedeyefor15minutesunderrunningwater.Thenconsultadoctor.AfterswallowingIfswallowed,rinsemouthwithwater（onlyifthepersonisconscious）Seekmedicaltreatment.InformationfordoctorShowtheproductlabelorthisMSDS.USA（Contd.onpage2）Page2/5MaterialSafetyDataSheetacc.toISO/DIS11014Printingdate08/28/2008Reviewedon08/26/2008Productname:BDMatrigelBasementMembraneMatrix（Contd.ofpage1）DR5FirefightingmeasuresSuitableextinguishingagentsCO2,ABCmultipurposedrychemicalorwaterspray.Fightlargerfireswithwatersprayoralcoholresistantfoam.Specialhazardscausedbythematerial,itsproductsofcombustionorresultinggases:Formationoftoxicgasesispossibleduringheatingorincaseoffire.Protectiveequipment:Wearself-containedrespiratoryprotectivedevice.6AccidentalreleasemeasuresPerson-relatedsafetyprecautions:Avoidformationofdust.Wearprotectiveclothing.Userespiratoryprotectivedeviceagainsttheeffectsoffumes/dust/aerosol.Measuresforenvironmentalprotection:Wipeupwithdampspongeormop.Measuresforcleaning/collecting:Pickupmechanically.Additionalinformation:SeeSection7forinformationonsafehand領(lǐng).SeeSection8forinformationonpersonalprotectionequipment.SeeSection13fordisposalinformation.7Hand領(lǐng)andstorageHand領(lǐng)Informationforsafehand領(lǐng):Preventformationofdust.Informationaboutprotectionagainstexplosionsandfires:Nospecialmeasuresrequired.StorageRequirementstobemetbystoreroomsandreceptacles:-20CInformationaboutstorageinonecommonstoragefacility:Storeawayfromoxidizingagents.Furtherinformationaboutstorageconditions:Storeincool,dryconditionsinwellsealedcontainers.8ExposurecontrolsandpersonalprotectionComponentswithlimitvaluesthatrequiremonitoringattheworkplace:Theproductdoesnotcontainanyrelevantquantitiesofmaterialswithcriticalvaluesthathavetobemonitoredattheworkplace.Additionalinformation:Theliststhatwerevalidduringthecreationwereusedasbasis.PersonalProtectiveEquipmentGeneralprotectiveandhygienicmeasuresTheusualprecautionarymeasuresforhand領(lǐng)chemicals首ldbefollowed.Washhandsbeforebreaksandattheendofwork.Avoidcontactwiththeeyesandskin.Breathingequipment:Usesuitablerespiratoryprotectivedeviceonlywhenaerosolormistisformed.（Contd.onpage3）USAPage3/5MaterialSafetyDataSheetacc.toISO/DIS11014Printingdate08/28/2008Reviewedon08/26/2008Productname:BDMatrigelBasementMembraneMatrix（Contd.ofpage2）DRProtectionofhands:Chemicalresistantgloves（i.e.nitrile,orequivalent）.Eyeprotection:Tightlysealedgoggles.Bodyprotection:Protectiveworkclothing（labcoat）.9Physicalandchemicalproperties:GeneralInformationForm:LiquidColor:AccordingtoproductspecificationOdor:CharacteristicChangeinconditionUndeterminedMeltingpoint/Meltingrange:NotdeterminedBoi領(lǐng)point/Boi領(lǐng)range:NotdeterminedFlashpoint:NotapplicableAutoigniting:Productisnotselfigniting.Dangerofexplosion:Productdoesnotpresentanexplosionhazard.Vaporpressure:NotapplicableDensity:NotdeterminedSolubilityin/MiscibilitywithWater:SolublepH-value:NotapplicableSolventcontent:Organicsolvents:≤0.0175%10StabilityandreactivityThermaldecomposition/conditionstobeavoided:Nodecompositionifusedaccordingtospecifications.Materialstobeavoided:Incompatiblematerial:strongoxidizers.DangerousreactionsNodangerousreactionsknownDangerousproductsofdecomposition:Nodangerousdecompositionproductsknown.11ToxicologicalinformationAcutetoxicity:Primaryirritanteffect:ontheskin:Noirritatingeffect.ontheeye:Noirritatingeffect.Sensitization:Nosensitizingeffectsknown.（Contd.onpage4）USAPage4/5MaterialSafetyDataSheetacc.toISO/DIS11014Printingdate08/28/2008Reviewedon08/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  GUIDELINESFORUSEPRODUCT:BDMatrigelhESC-qualifiedMatrix,5mlvialCATALOGNUMBER:354277BACKGROUND:Basementmembranesarecontinuoussheetsofspecializedextracellularmatrixthatarefoundatthedermal-epidermaljunction,atthebaseofalllumen-liningepitheliathroughoutthedigestive,respiratory,reproductiveandurinarytractsandthatunderlieparenchymaofendocrineandexocrineglands.BDMatrigelMatrixisasolublebasementmembraneextractoftheEngelbreth-Holm-Swarm（EHS）tumorthatgelsatroomtemperaturetoformagenuinereconstitutedbasementmembrane.1ThemajorcomponentsofMatrigelMatrixarelaminin,collagenIV,entactinandheparansulfateproteoglycan.2-3Growthfactors,collagenases,plasminogenactivatorsandotherundefinedcomponentshavealsobeenreportedinMatrigelMatrix.4-5STEMCELLS:Historically,hEScellderivationandculturingtechniquesutilizedserumand/ormouseembryonicfibroblast（MEF）feederlayers.6AnidealenvironmentforhEScellresearchconsistsofbothacellculturesurfacespecificallyqualifiedforhEScells,andaserum-free,definedmedium.BDMatrigelhESC-qualifiedMatrixandSTEMCELLTechnologies’mTeSR1（developedunderlicensefromtheWiCellResearchInstitute7）,ahighqualitysurfaceandmediumcombination,createthefirstcompleteenvironmenttosupportfeeder-independentexpansionofhEScells.BDMatrigelhESC-qualifiedMatrixisanoptimizedsurfaceforyourstemcellresearch.IthasbeenqualifiedasmTeSR1-compatiblebySTEMCELLTechnologies,eliminatingtheneedfortime-consumingscreening,whileprovidingthereproducibilityandconsistencyessentialforyourhEScellresearch.Whencoupledwithavarietyofculturemedia,MatrigelMatrixhasbeenwidelyacceptedasanalternativesubstrateforthecultureofhEScells.8-9ThemTeSR1formulationisdefinedandserum-free,andhasbeendesignedtomaintainandexpandhEScellsinanundifferentiatedstatewhenusedwithMatrigelMatrixasasubstrate.Itdoesnotrequireanyfurtheradditionofgrowthfactorsorsupplements.ThemTeSR1formulationandMatrigelMatrixhavebeenshowntobeasuccessfulcombinationforculturingdifferenthEScelllinesforupto20passages.10CellsmaintainedinmTeSR1expresshighlevelsofpluripotencymarkerssuchasOct-3/4andSSEA-3,andpluripotencyofcellsmaintainedinmTeSR1hasalsobeendemonstratedbytheabilityofthesecellstodifferentiateintoallthreegermlayersintheteratomaassay.7,11SOURCE:Engelbreth-Holm-Swarm（EHS）MouseTumorFORMULATION:Dulbecco'sModifiedEagle'sMediumwith50μg/mlgentamycinMatrigelhESC-qualifiedMatrixiscompatiblewithallculturemediaSTABILITY:Stableforaminimumofthreemonthsfromdayofshipmentwhenstoredat-20°CStableforaminimumofsixmonthswhenstoredinaliquotsat-70°CRECONSTITUTIONANDUSE:ColorvariationsmayoccurinfrozenorthawedvialsofBDMatrigelhESC-qualifiedMatrix,rangingfromstrawyellowtodarkredduetotheinteractionofcarbondioxidewiththebicarbonatebufferandphenolred.Thisisnormal,doesnotaffectproductefficacy,andwilldisappearuponequilibrationwith5%CO2.ThawMatrigelonice.Oncethawed,swirlthevialtoensuretheMatrigelisevenlydispersed.Spraytopofvialwith70%ethanolandairdry.Keepproductoniceandhandleusingsteriletechnique.Dispensematerialintoappropriatealiquots,usingpre-cooledtubes,andrefreezeimmediately.Avoidmultiplefreezethaws.DONOTSTOREINFROST-FREEFREEZER.CAUTION:BDMatrigelhESC-qualifiedMatrixwillgelrapidlyat22oCto35oC.Thawovernightat4oConice.GelledMatrigelmaybere-liquifiedifplacedat4°Conicefor24-48hours.[詳細(xì)]

  2018-09-02 10:00

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• BD 356234356235 |價(jià)格|說明書

  BDMatrigelTM基質(zhì)基底膜促銷356234|354234|3562352023/5ml3859/10ml16509/5*10ml基底膜簡介：中文名稱：基底膜英文名稱：basementmembrane;basallamina其他名稱：基板定義：上皮細(xì)胞下面特化的細(xì)胞外基質(zhì)，由Ⅳ型膠原、層粘連蛋白及硫酸乙酰肝素蛋白聚糖等構(gòu)成的網(wǎng)狀結(jié)構(gòu)。對上皮細(xì)胞、內(nèi)皮細(xì)胞等的生命活動具有重要影響。BDMatrigelTM基質(zhì)基底膜促銷356234|354234|356235BDMatrigel基底膜/基質(zhì)膠的應(yīng)用：1）促進(jìn)多種細(xì)胞的分化；2）形成基底膜三維膠質(zhì)結(jié)構(gòu)；3）提高人腫瘤細(xì)胞在裸鼠上的生長速度；4）建立評估腫瘤細(xì)胞侵襲能力的模型BDMatrigel基底膜/基質(zhì)膠的詳細(xì)說明：BDMatrigel是從富含胞外基質(zhì)蛋白的EHS小鼠腫瘤中提取出基底膜基質(zhì)，其主要成分有層粘連蛋白、Ⅳ型膠原、巢蛋白、硫酸肝素糖蛋白，還包含生長因子和基質(zhì)金屬蛋白酶等。在室溫條件下，Matrigel聚合形成具有生物學(xué)活性的三維基質(zhì)，模擬體內(nèi)細(xì)胞基底膜的結(jié)構(gòu)、組成、物理特性和功能，有利于體外細(xì)胞的培養(yǎng)和分化，可用于對細(xì)胞形態(tài)、生化功能、遷移、侵染和基因表達(dá)等的研究。BDMatrigel基底膜/基質(zhì)膠形成的三維培養(yǎng)基質(zhì)，可促進(jìn)上皮細(xì)胞、肝細(xì)胞、Sertoli細(xì)胞、黑色素瘤細(xì)胞、血管內(nèi)皮細(xì)胞、甲狀腺細(xì)胞及毛囊細(xì)胞等的貼壁與分化。同時(shí)，Matrigel還能影響乳腺上皮細(xì)胞的蛋白表達(dá)，支持外周神經(jīng)的新生和牛輸卵管上皮細(xì)胞的分化。不同配方的BDMatrigel可以滿足不同的實(shí)驗(yàn)要求。低生長因子（GFR）的Matrigel基底膜基質(zhì)適用于對基質(zhì)成分要求嚴(yán)格的實(shí)驗(yàn)，如細(xì)胞信號通路和細(xì)胞因子的研究等。不含酚紅的Matrigel適用于熒光檢測或顯色反應(yīng)等分析。高濃度的Matrigel適用于研究血管生成、腫瘤細(xì)胞遷移和體內(nèi)腫瘤模型的建立等。BDMatrigelTM基質(zhì)基底膜促銷356234|354234|356235細(xì)胞與基底膜間的相互作用是調(diào)控細(xì)胞行為的重要因素之一。而使用BDMatrigel系列的細(xì)胞外基質(zhì)培養(yǎng)的細(xì)胞可以有著類似體內(nèi)環(huán)境的良好分化表現(xiàn)。作為**個生產(chǎn)一次性無菌實(shí)驗(yàn)室用品的公司，BD生物科學(xué)在世界范圍內(nèi)都被認(rèn)為是研究者提供優(yōu)質(zhì)細(xì)胞培養(yǎng)產(chǎn)品的引領(lǐng)者。50年來，BD一直致力于提高您實(shí)驗(yàn)的可靠性，一致性和便捷性。每一BDFalcon組織培養(yǎng)產(chǎn)品都經(jīng)由敏感的克隆形成實(shí)驗(yàn)開發(fā)。BD更將含氮的正電荷功能集團(tuán)引入具有含氧負(fù)電荷功能集團(tuán)的傳統(tǒng)TC表面，生產(chǎn)出可改善多種類型細(xì)胞貼壁和分化的BDPrimaria細(xì)胞培養(yǎng)用品。每一批次BDPrimaria產(chǎn)品樣本都經(jīng)過化學(xué)分析電子掃描分析。MatrigelTM基本操作薄膠成膠方法凍融后，用預(yù)冷的移液槍頭混勻MatrigelTM基質(zhì)成勻漿狀將需要使用的培養(yǎng)板置于冰上，加入濃度為50μL/cm生長面積的Matrigel基質(zhì)在37℃放置30分鐘，即可使用厚膠成膠方法凍融后，用預(yù)冷的移液槍頭混勻MatrigelTM基質(zhì)成勻漿狀。將需要使用的培養(yǎng)板置于冰浴，將培養(yǎng)的細(xì)胞與MatrigelTM基質(zhì)混合，用移液槍頭使其懸浮于基質(zhì)中。加入濃度為150－200μL/cm生長面積的Matrigel基質(zhì)在37℃放置30分鐘，可成膠?？梢约尤爰?xì)胞培養(yǎng)的基質(zhì)，也可使細(xì)胞直接生長在膠表面薄層包被方法凍融后，用預(yù)冷的移液槍頭混勻Matrigel基質(zhì)成勻漿狀。根據(jù)使用需要，采用無血清培養(yǎng)基稀釋Matrigel基質(zhì)。根據(jù)實(shí)驗(yàn)需要確定**包被濃度。將稀釋的Matrigel基質(zhì)包被于所需的培養(yǎng)器皿中，包被量至少覆蓋整個器皿的生長表面。室溫下孵育1小時(shí)。去除未結(jié)合的Matrigel，用無血清培養(yǎng)基輕輕地沖洗現(xiàn)貨供應(yīng)，保證質(zhì)量。我們將提供Z合理的價(jià)格。BDMatrigelTM基質(zhì)基底膜促銷356234|354234|356235【021-54100800】上海索來寶生物科技有限公司公司網(wǎng)址：http://www.shsolarbio.comhttp://bioing.biogo.net/聯(lián)系人：馬先生（先生）部門（職位）：銷售（經(jīng)理）手機(jī)號碼：15021200052電話：86-021-64855665傳真：86-021-54261506所在地：上海公司地址：徐匯區(qū)欽江路15號1405郵政編碼：200233郵件：shsolarbio@126.com相關(guān)產(chǎn)品附錄產(chǎn)品描述規(guī)格特點(diǎn)貨號報(bào)價(jià)BDMatrigelTM基質(zhì)基底膜5ml標(biāo)準(zhǔn)型356234202310ml　35423438595×10ml　35623516509BDMatrigelTM基底膜基質(zhì)，無酚紅10ml標(biāo)準(zhǔn)型3562373034BDMatrigelTM基底膜基質(zhì)，GFR，低生長因子5ml標(biāo)準(zhǔn)型356230232510ml標(biāo)準(zhǔn)型3542304533BDMatrigelTM基底膜基質(zhì)，GFR，無酚紅10ml標(biāo)準(zhǔn)型3562314883MATRIGELMATRIXHESC-QUALIFIED5ml　3542772279COLLAGENIRATTAILNATURAL100MG　3542361093CHAMBERMATRIGELINVASION24WELLDI　　3544804301運(yùn)輸和儲存-20℃儲存，干冰運(yùn)輸Matrigel基質(zhì)會有色差變化，是由于酚紅和碳酸氫鹽與CO2作用引起的，但是與5％CO2平衡后色差即會減少?。?！。[詳細(xì)]

  2018-09-02 10:00

  產(chǎn)品樣冊

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• BD 354230 相關(guān)說明

  1Identificationofsubstance:Productname:BDMatrigelBasementMembraneMatrixCatalognumber:354230,354234,354277,356230,356231,356234,356235,356237Manufacturer/Supplier:BDBiosciences-DiscoveryLabware2OakParkBedford,MA01730Tel:877.232.8995Informationdepartment:Forfurtherinformation,pleasecallTechnicalServicesat（877）232-8995Emergencyinformation:Incaseofachemicalemergency,spill,fire,exposure,oraccidentcontactBDDiscoveryLabwareat800.334.5229,orChemTrecat800.424.9300.2Composition/Dataoncomponents:ChemicalcharacterizationDescription:Mixtureofthesubstanceslistedbelowwithnonhazardousadditions.Dangerouscomponents:CASNO.67-66-3Chloroform≤0.02CASNO.Description%chloroformCarc.Cat.3;Xn,Xi;R22-38-40-48/20/22Danger:Flame2.6/2Warning:Healthhazard3.6/2,3.9/2;Exclamationmark3.1.O/4,3.2/23HazardsidentificationHazarddescription:Thisproductcontainsnohazardousconstituents,ortheconcentrationofallchemicalconstituentsarebelowtheregulatorythresholdlimitsdescribedbyOccupationalSafetyHealthAdministrationHazardCommunicationStandard29CFR1910.1200andtheEuropeanDirective91/155/EEC,and93/112/EC.ClassificationsystemTheclassificationwasmadeaccordingtothelatesteditionsofinternationalsubstanceslists,andexpandeduponfromcompanyandliteraturedata.NFPAratings（scale0-4）000Health=0Fire=0Reactivity=04FirstaidmeasuresAfterinhalationSupplyfreshair;consultdoctorincaseofcomplaints.AfterskincontactImmediatelywashwithwaterandsoapandrinsethoroughly.AftereyecontactRinseopenedeyefor15minutesunderrunningwater.Thenconsultadoctor.AfterswallowingIfswallowed,rinsemouthwithwater（onlyifthepersonisconscious）Seekmedicaltreatment.InformationfordoctorShowtheproductlabelorthisMSDS.354230-MSDS-USAUSA現(xiàn)貨促銷021-54100800[詳細(xì)]

  2018-09-02 10:00

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• BD RevTet System使用說明

  BDRevTetSystem使用說明pRevTet-On，pRevTet-Off，pRevTet-Off-IN，pRevTREIntroductionTheBDRevTet-OnTM&BDRevTet-OffTMSystemscombinetheadvantagesofretrovirus-mediatedgenetransferwiththeproventetracycline-regulatedcontrolofBDTet-OnTMandBDTet-OffTMGeneExpressionSystems.BDRevTetTMallowsthefastandefficientestablishmentofregulatedgeneexpressionsystemsinawidevarietyofcelltypes.EachRevTetSystemisacompleteretroviralgeneexpressionsystemcontainingaretroviralregulator（r）tTAvector,aretroviralresponsevector,acontrolvector,andapackagingcellline.LikeourBDRetro-XTMSystem（#631508）,theRevTetSystemscontaintheBDRetroPackTMPT67PackagingCellLine（#631510）forthesafe,efficientproductionofinfectious,replication-incompetentretrovirus.TheRevTetSystemsprovideapowerfulandconvenientapproachtosettingupthetetracycline（Tc）-inducible,high-levelgeneexpressionsystemsfirstdescribedbyGossenandBujard（1992）.Retroviral-mediatedtet-regulationTheTetSystemsarebasedontwoelementsfromthetetoperonoftheE.coliTn10transposontheTetrepressorprotein（TetR）andthetetoperatorDNAsequence（tetO）.ThegenetobeexpressedisclonedintothepRevTREresponsevectordownstreamofthetetracycline-responsiveelement（TRE）,whichcontainssevendirectrepeatsofthe42-bptetOsequenceandtheminimalimmediateearlypromoterofcytomegalovirus（PminCMV）.Thetwosystemsdifferintheirregulatoryvector（Figure1）.TheRevTet-OffSystemusesthepRevTet-OffVector,whichcontainsthetTAregulatoryelement,afusionofTetRandtheVP16activationdomain.TheBDRevTet-OnTMSystemusespRevTet-On,basedonthereversetTA（rtTA）.BothsystemsusethepRevTREresponsevector.AlltheRevTetVectorsarederivedfrompLNCX,aBDRetro-XTMvectorcapableofproducinghigh-titervirusintheappropriatepackagingcelllines.IntheRevTet-OffSystem,tTAbindstheTREandactivatestranscriptionintheabsenceofTcortheTcderivativedoxycycline（Dox）.Thus,asTcorDoxisaddedtotheculturemedium,transcriptionisturnedoffinadose-dependentmanner（Figure1）.Conversely,theRevTet-OnSystemallowsgeneexpressiontobeactivatedbytheadditionofDox.Thesetwocomplementarysystemsallowyoutochoosetheonethatbestsuitsyourneeds.BenefitsandapplicationsofTc-mediatedinductionInmostinduciblemammaliangeneexpressionsystems（e.g.,inductionbyheavymetals,steroidhormones,orheatshock）,inductionisnonspecificandexpressionlevelscannotbepreciselyregulated.Inaddition,thesesystemsaregenerallyleakyinthe“off”state,andtheinducingagentitselfmaybecytotoxicorhavepleiotropiceffectsonresults.Incontrast,regulationofgeneexpressionbytheheterologousbacterialcontrolelementsintheRevTetSystemsisveryspecific.Furthermore,thelevelsofTcorDoxrequiredforthefullrangeofgeneexpressionarenotcytotoxicandhavenosignificanteffectoncellproliferationoranimalgrowth,evenwithcontinuoustreatment（Bohletal.,1997;Mayfordetal.,1996）.TheuseofseparateregulatoryandresponsevectorsintheRevTetSystems質(zhì)粒圖譜:[詳細(xì)]

  2018-09-29 10:03

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• BD培養(yǎng)基2012價(jià)目表

  上海北諾生物科技有限公司www.bnbiotech.com上海市徐匯區(qū)宜山路520號中華門大廈18樓D座電話：021-61496710傳真：021-57633753聯(lián)系人：周經(jīng)理，劉經(jīng)理[詳細(xì)]

  2018-10-14 10:00

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• Application: BD BioCoat? 產(chǎn)品應(yīng)用

  Application: BD BioCoat? 產(chǎn)品應(yīng)用[詳細(xì)]

  2024-09-14 21:08

  課件

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• BD FACS VERSE 流式細(xì)胞儀

  BD FACS VERSE 流式細(xì)胞儀[詳細(xì)]

  2025-07-29 14:07

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