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Reads在生物基因里是何物?可否形象解釋一下?或者給個(gè)專業(yè)的翻譯……

朕200055 2012-02-19 19:43:07 577  瀏覽
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  • af05522 2012-02-20 00:00:00
    就是一段測(cè)序的讀數(shù),和測(cè)斜儀的讀長(zhǎng)相關(guān)。 如:將基因組打斷成200 bp的片段,reads為10 bp,讀出的序列為ATCTGTCCTA,那么該序列就是read。很多read就是reads咯

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  • 小啦啦gg 2017-11-25 00:07:33
    reads不是基因基因組中的組成,實(shí)際是一小段短的測(cè)序片段,是高通量測(cè)序儀產(chǎn)生的測(cè)序數(shù)據(jù),對(duì)整個(gè)基因組進(jìn)行測(cè)序,就會(huì)產(chǎn)生成百上千萬的reads,然后將這些reads拼接起來就能獲得基因組的全序列了~發(fā)展到現(xiàn)在,高通量測(cè)序技術(shù)已經(jīng)可以應(yīng)用到轉(zhuǎn)錄組的研究,不同片段reads量(就是這些小的測(cè)序片段)不同可以代表不同的表達(dá)水平。更具體可以關(guān)注一下NAR(核算研究)上面的NGS(Next Generation Sequencing)子期刊。

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