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2018-12-07 07:21發(fā)布了問答
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成纖維細(xì)胞dna提取用什么試劑盒比較好
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2018-11-22 12:49發(fā)布了問答
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腸道微生物基因組測序需要多少錢
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2018-11-20 22:06發(fā)布了問答
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無創(chuàng)解惑之無創(chuàng)DNA檢測和無創(chuàng)親子鑒定的區(qū)別
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2018-01-08 07:12發(fā)布了問答
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pcr測序結(jié)果怎么看DNA
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2017-11-22 00:25發(fā)布了問答
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atp水解產(chǎn)物能作為合成dna的原料么
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2017-10-20 02:43發(fā)布了問答
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fish以后挑出來的細(xì)胞會(huì)不會(huì)影響rna抽提
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2017-10-03 05:51發(fā)布了問答
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高通亮測序 樣本準(zhǔn)備
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2017-06-15 04:09發(fā)布了問答
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做無創(chuàng)DNA大概多長時(shí)間出結(jié)果
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2017-05-18 18:31發(fā)布了問答
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紅外氣體分析儀和紫外線分析儀哪個(gè)好
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2017-05-07 00:53發(fā)布了問答
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瑪吉斯324和340差別大嗎
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2017-01-12 21:38發(fā)布了問答
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如何評(píng)價(jià)蛋白質(zhì)純化方法的優(yōu)劣
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2016-04-27 20:19發(fā)布了問答
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基因控制蛋白質(zhì)合成的過程,基因的空間結(jié)構(gòu)為什么會(huì)改變
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2016-03-01 04:44發(fā)布了問答
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轉(zhuǎn)錄組測序中的比對率是什么意思
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2012-12-13 02:58發(fā)布了問答
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論文摘要 英譯漢 ~~給很多分很多分噢!!
- Abstract: To isolate anti-salt stress genes from mangrove plants, a cDNA library of R. stylosa roots was constructed and screened for stress-related genes by polymerase chain reaction (PCR)- based suppressive subtractive hybridization (SSH... Abstract:
To isolate anti-salt stress genes from mangrove plants, a cDNA library of R. stylosa roots was constructed and screened for stress-related genes by polymerase chain reaction (PCR)- based suppressive subtractive hybridization (SSH). The mangrove seedlings were grown for 8 months under two conditions: exposure to water with 3% salt and exposure to fresh water. cDNA of seedlings exposed to water with 3% salt was used as a tester and cDNA for freshwater germination was used as a driver. We isolated and sequenced 240 up-regulated expressed sequence tags (ESTs) from the SSH library. Among these up-regulated ESTs, 48 unique clones were putatively identified and classified into ten functional categories, such as cell rescue and defense, secondary metabolism, protein synthesis, and metabolism. Fifteen genes from different categories were selected and their expression was studied by real-time RT-PCR. Significantly increased expression levels were confirmed for 13 of these 15 transcripts, which suggest that these genes contribute to the salt tolerance of this plant. Among them, two transcription factors and several genes involved in isoprenoid biosynthesis were identified from mangrove trees as salt tolerance genes for the first time. The physiological significance of the increased expression of these genes in the long-term adaptation of mangrove trees to salt stress is discussed. 展開
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2011-03-22 12:11發(fā)布了問答
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第四代基因測序會(huì)是什么?
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2008-12-03 12:36發(fā)布了問答
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大腸桿菌是怎樣生產(chǎn)胰島素的
- 眾所周知,大腸桿菌是原核生物,細(xì)胞器只有核糖體而沒有內(nèi)質(zhì)網(wǎng)和高爾基體;胰島素是分泌蛋白,在核糖體上合成的只是沒有活性的多肽鏈,必須經(jīng)過內(nèi)質(zhì)網(wǎng)和高爾基體再加工才有活性;基因工程將人的胰島素基因?qū)氪竽c桿菌中,實(shí)現(xiàn)了胰島素的工廠化生產(chǎn)。這怎么解... 眾所周知,大腸桿菌是原核生物,細(xì)胞器只有核糖體而沒有內(nèi)質(zhì)網(wǎng)和高爾基體;胰島素是分泌蛋白,在核糖體上合成的只是沒有活性的多肽鏈,必須經(jīng)過內(nèi)質(zhì)網(wǎng)和高爾基體再加工才有活性;基因工程將人的胰島素基因?qū)氪竽c桿菌中,實(shí)現(xiàn)了胰島素的工廠化生產(chǎn)。這怎么解釋?
請用比較簡單而詳細(xì)的語言回答。謝謝。 展開